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PR01893

Identifier
WNT10PROTEIN  [View Relations]  [View Alignment]  
Accession
PR01893
No. of Motifs
5
Creation Date
13-MAY-2003
Title
Wnt-10 protein signature
Database References
PRINTS; PR01349 WNTPROTEIN
MIM; 601906
Literature References
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
 
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
 
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.REV. 33 1-12 (2000).
 
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
 
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
 
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
 
7. KIRIKOSHI, H., SEKIHARA, H. AND KATOH, M.
WNT10A and WNT6, clustered in human chromosome 2q35 region with head-to-tail
manner, are strongly coexpressed in SW480 cells.
BIOCHEM.BIOPHYS.RES.COMMUN. 283 798-805 (2001).
 
8. WANG, J. AND SHACKLEFORD, G.M.
Murine Wnt10a and Wnt10b: cloning and expression in developing limbs, face 
and skin of embryos and in adults.
ONGOGENE 13 1537-1544 (1996).

Documentation
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidney,
regulation of mammary gland proliferation, and Alzheimer's disease [3,4].
 
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of 
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been 
identified in vertebrates, with multiple subtypes within some classes.
 
Two subtypes of the Wnt-10 protein exist, designated Wnt-10a and Wnt-10b. In
mouse, these proteins share 62% sequence identity, compared to 59% in human 
[7,8]. Their expression pattern indicates that they function in tissues 
responsible for the formation and maturation of red blood cells.
 
WNT10PROTEIN is a 5-element fingerprint that provides a signature for the 
Wnt-10 proteins. The fingerprint was derived from an initial alignment of 5 
sequences: the motifs were drawn from conserved regions spanning the full
alignment length, focusing on those sections that characterise Wnt-10 
proteins but distinguish them from other Wnt subtypes - motifs 1-5 reside in 
the conserved domain between the highly conserved cysteines. Two iterations 
on SPTR40_22f were required to reach convergence, at which point a true set 
comprising 6 sequences was identified. A single partial match was also 
found, P79753, Wnt10b from Fugu rubripes that matches motifs 1, 2 and 5.
Summary Information
   6 codes involving  5 elements
0 codes involving 4 elements
1 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
566666
400000
311001
200000
12345
True Positives
P79856        WN1A_BRARE    WN1A_HUMAN    WN1A_MOUSE    
WN1B_HUMAN WN1B_MOUSE
True Positive Partials
Codes involving 3 elements
P79753
Sequence Titles
P79856      PWnt-10a - Pleurodeles waltlii (Iberian ribbed newt). 
WN1A_BRARE Wnt-10a protein precursor - Brachydanio rerio (Zebrafish) (Danio rerio).
WN1A_HUMAN WNT-10A protein precursor - Homo sapiens (Human).
WN1A_MOUSE WNT-10A protein precursor - Mus musculus (Mouse).
WN1B_HUMAN WNT-10B protein precursor (WNT-12) - Homo sapiens (Human).
WN1B_MOUSE WNT-10B protein precursor (WNT-12) - Mus musculus (Mouse).

P79753 Wnt10b - Fugu rubripes (Japanese pufferfish) (Takifugu rubripes).
Scan History
SPTR40_22f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
NDILGLRLPPEP WN1A_MOUSE 42 42 -
NDILDLRLPPEP WN1A_HUMAN 42 42 -
NEILGLKIPFDP WN1A_BRARE 79 79 -
NEILGLKLPGEP WN1B_HUMAN 29 29 -
NEILGLKLPGEP WN1B_MOUSE 29 29 -

Motif 2 width=11
Element Seqn Id St Int Rpt
LETRNKVPYES WN1A_MOUSE 110 56 -
LETRNKIPYES WN1A_HUMAN 110 56 -
LETRNKIPYES WN1A_BRARE 147 56 -
LEGGGRLPHHS WN1B_HUMAN 97 56 -
LEGGGRLPHHS WN1B_MOUSE 97 56 -

Motif 3 width=11
Element Seqn Id St Int Rpt
RGKGLSHGVPE WN1A_MOUSE 184 63 -
RGKGLSHGVPE WN1A_HUMAN 184 63 -
RGKGMVHGVME WN1A_BRARE 221 63 -
RGKSFPHSLPS WN1B_HUMAN 169 61 -
RGKTFPISQPS WN1B_MOUSE 169 61 -

Motif 4 width=15
Element Seqn Id St Int Rpt
ILPASPGLQDSWEWG WN1A_MOUSE 198 3 -
LPTASPGLQDSWEWG WN1A_HUMAN 198 3 -
FPAEALGPQDSWEWG WN1A_BRARE 233 1 -
GSSPSPGPQDTWEWG WN1B_HUMAN 183 3 -
GSVPSPGPQDTWEWG WN1B_MOUSE 183 3 -

Motif 5 width=13
Element Seqn Id St Int Rpt
GLRRRASHSDLVY WN1A_MOUSE 326 113 -
GPRRRASPADLVY WN1A_HUMAN 326 113 -
THRRRANINDLVY WN1A_BRARE 351 103 -
RLRPRRLSGELVY WN1B_HUMAN 298 100 -
RLRPRRLSGELVY WN1B_MOUSE 298 100 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
NDILGLRLPPEP WN1A_MOUSE 42 42 -
NDILDLRLPPEP WN1A_HUMAN 42 42 -
NEILGLKIPFDP WN1A_BRARE 79 79 -
NEILGLKIPSDP P79856 26 26 -
NEILGLKLPGEP WN1B_HUMAN 29 29 -
NEILGLKLPGEP WN1B_MOUSE 29 29 -

Motif 2 width=11
Element Seqn Id St Int Rpt
LETRNKVPYES WN1A_MOUSE 110 56 -
LETRNKIPYES WN1A_HUMAN 110 56 -
LETRNKIPYES WN1A_BRARE 147 56 -
LETKNKIPYES P79856 94 56 -
LEGGGRLPHHS WN1B_HUMAN 97 56 -
LEGGGRLPHHS WN1B_MOUSE 97 56 -

Motif 3 width=11
Element Seqn Id St Int Rpt
RGKGLSHGVPE WN1A_MOUSE 184 63 -
RGKGLSHGVPE WN1A_HUMAN 184 63 -
RGKGMVHGVME WN1A_BRARE 221 63 -
RGKGMVHGVLE P79856 168 63 -
RGKSFPHSLPS WN1B_HUMAN 169 61 -
RGKTFPISQPS WN1B_MOUSE 169 61 -

Motif 4 width=15
Element Seqn Id St Int Rpt
ILPASPGLQDSWEWG WN1A_MOUSE 198 3 -
LPTASPGLQDSWEWG WN1A_HUMAN 198 3 -
FPAEALGPQDSWEWG WN1A_BRARE 233 1 -
MPSEVPGPQETWEWG P79856 180 1 -
GSSPSPGPQDTWEWG WN1B_HUMAN 183 3 -
GSVPSPGPQDTWEWG WN1B_MOUSE 183 3 -

Motif 5 width=13
Element Seqn Id St Int Rpt
GLRRRASHSDLVY WN1A_MOUSE 326 113 -
GPRRRASPADLVY WN1A_HUMAN 326 113 -
THRRRANINDLVY WN1A_BRARE 351 103 -
PHRRRSSINSLVY P79856 298 103 -
RLRPRRLSGELVY WN1B_HUMAN 298 100 -
RLRPRRLSGELVY WN1B_MOUSE 298 100 -