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PR01699

Identifier
ORGNOHGLYASE  [View Relations]  [View Alignment]  
Accession
PR01699
No. of Motifs
5
Creation Date
25-MAR-2002
Title
Organomercurial lyase signature
Database References

UMBBD; e0055
Literature References
1. FOSTER, T.J. 
The genetics and biochemistry of mercury resistance. 
CRIT.REV.MICROBIOL. 15(2) 117-140 (1987).
 
2. BEGLEY, T.P., WALTS, A.E. AND WALSH, C.T. 
Bacterial organomercurial lyase: overproduction, isolation, and 
characterisation. 
BIOCHEMISTRY 25(22) 7186-7192 (1986).
 
3. MISRA, T.K. 
Bacterial resistances to inorganic mercury salts and organomercurials. 
PLASMID 27(1) 4-16 (1992).
 
4. KIYONO, M., OMURA, T., INUZUKA, M., FUJIMORI, H. AND PAN-HOU, H.
Nucleotide sequence and expression of the organomercurial-resistance 
determinants from a Pseudomoas K-62 plasmid pMR26. 
GENE 189 151-157 (1997).
 
5. HUANG, C.C., NARITA, M., YAMAGATA, T. AND ENDO, G.
Identification of three merB genes and characterisation of a broad-spectrum 
mercury resistance module encoded by a class II transpoon of Bacillus 
megaterium strain MB1. 
GENE 239 361-366 (1999).

Documentation
Mercury is a highly toxic metal. Toxicity can result from three different
mercurial forms: elemental, inorganic ion and organomercurial compounds. The
ability of bacteria to detoxify mercurial compounds by reduction and
volatilisation is conferred by the Mer genes, which are usually plasmid
encoded (although chromosome resistance determinants have also occasionally
been identified) [1,3,4,5]. Organomercurial lyase (MerB) mediates the first
of the two steps in the microbial detoxification of organomercurial salts
(the other catalysed by mercuric reductase). 
 
Organomercurial lyase catalyses the protonolysis of the C-Hg bond in a wide
range of organomercurial salts (primary, secondary, tertiary, alkyl, vinyl,
allyl and aryl) to Hg(II) and the respective organic compound [2]:
 
			RHg(+) + H(+) = RH + Hg(2+)
 
Hg(II) is subsequently detoxified by mercuric reductase. 
 
The enzyme has been purified to homogeneity in E.coli and has been found
to be a 22.4kDa monomer with no detectable cofactors or metal ions [2].
 
ORGNOHGLYASE is a 5-element fingerprint that provides a signature for
organomercurial lyases. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved regions
spanning the full alignment length. Two iterations on SPTR40_18f were
required to reach convergence, at which point a true set comprising 13
sequences was identified. A single partial match was also found, Q9S4I3,
an organomercurial lyase from E.coli that fails to make a significant match
with motif 5.
Summary Information
  13 codes involving  5 elements
1 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
51313131313
411110
300000
200000
12345
True Positives
MERB_BACSR    MERB_SERMA    MERB_STAAU    MERB_STRLI    
O07303 P77072 Q9F3W5 Q9F3W6
Q9L7K6 Q9S4I1 Q9S4I2 Q9WWL2
Q9Z3Y8
True Positive Partials
Codes involving 4 elements
Q9S4I3
Sequence Titles
MERB_BACSR  Alkylmercury lyase (EC 4.99.1.2) (Organomercurial lyase) - Bacillus sp. (strain RC607). 
MERB_SERMA Alkylmercury lyase (EC 4.99.1.2) (Organomercurial lyase) - Serratia marcescens.
MERB_STAAU Alkylmercury lyase (EC 4.99.1.2) (Organomercurial lyase) - Staphylococcus aureus.
MERB_STRLI Alkylmercury lyase (EC 4.99.1.2) (Organomercurial lyase) - Streptomyces lividans.
O07303 ORGANOMERCURIC LYASE - Pseudomonas sp.
P77072 ALKYLMERCURY LYASE (EC 4.99.1.2) (ORGANOMERCURIAL LYASE) - Escherichia coli.
Q9F3W5 ORGANOMERCURIATLIASE - Pseudomonas sp. ED23-33.
Q9F3W6 ORGANOMERCURIATLIASE - Pseudomonas sp. ED23-33.
Q9L7K6 MERB - Streptomyces sp. CHR28.
Q9S4I1 ORGANOMERCURIAL LYASE - Escherichia coli.
Q9S4I2 ORGANOMERCURIAL LYASE - Escherichia coli.
Q9WWL2 ORGANIC MERCURIC LYASE MERB2 - Bacillus sp. (strain RC607), Bacillus megaterium, and Clostridium butyricum.
Q9Z3Y8 MERB2 - Pseudomonas sp. K-62.

Q9S4I3 ORGANOMERCURIAL LYASE - Escherichia coli.
Scan History
SPTR40_18f 2  150  NSINGLE    
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
GRPVSRTTLAGILD MERB_SERMA 34 34 -
GRPVSRTTLAGILD P77072 34 34 -
GDPVPVEDIAAETG MERB_STAAU 37 37 -
GESVTIEDMATTTG MERB_BACSR 39 39 -
GRPVTVEQIAQATD MERB_STRLI 39 39 -
GDLYHAKSSPAQLG O07303 37 37 -
GQAVTESSLINQTG Q9WWL2 36 36 -

Motif 2 width=15
Element Seqn Id St Int Rpt
GLTLRETSYVFEIDD MERB_SERMA 75 27 -
GLTLRETSYVFEIDD P77072 75 27 -
GLTLFPTPHRFEVDG MERB_STAAU 78 27 -
GLTLIPTPHHFTVDG MERB_BACSR 80 27 -
GLTQNPTPHHFEVDG MERB_STRLI 80 27 -
GLNLRDTSHVFEVDG O07303 78 27 -
GLSIIPTTNQIHLGG Q9WWL2 81 31 -

Motif 3 width=10
Element Seqn Id St Int Rpt
LYAWCALDTL MERB_SERMA 92 2 -
LYAWCALDTL P77072 92 2 -
LYAWCALDTL MERB_STAAU 95 2 -
LYAWCALDTL MERB_BACSR 97 2 -
LYTWCALDTL MERB_STRLI 97 2 -
LYTWCVLDTL O07303 95 2 -
LFAWCAISTL Q9WWL2 98 2 -

Motif 4 width=12
Element Seqn Id St Int Rpt
CAATGAPVSLTV MERB_SERMA 117 15 -
CAATGAPVSLTV P77072 117 15 -
CHGTGKSVRLTV MERB_STAAU 120 15 -
CHSTGEPIRLNV MERB_BACSR 122 15 -
CHATGTPVRLTV MERB_STRLI 122 15 -
CAATGRPITLTV O07303 119 14 -
CAYTGEPIEVTV Q9WWL2 123 15 -

Motif 5 width=16
Element Seqn Id St Int Rpt
HFFASVPTAEDWASKH MERB_SERMA 163 34 -
HFFASVPTAEDWASKH P77072 163 34 -
HFFSSPSAAQDWLNQH MERB_STAAU 166 34 -
HFFSSPNAAEDWLDQH MERB_BACSR 168 34 -
HFFATPDAGKGWLEEH MERB_STRLI 168 34 -
HFFASPSIANSWASTH O07303 165 34 -
HYFSSVEHANKWKEEH Q9WWL2 169 34 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
GRPVSRTTLAGILD MERB_SERMA 34 34 -
GRPVSRTTLAGILD P77072 34 34 -
GRPVSRTTLAGILD Q9S4I1 34 34 -
GRPVSRTTLAGILD Q9S4I2 34 34 -
GRPVSRTTLAGILD Q9Z3Y8 34 34 -
GDPVPVEDIAAETG MERB_STAAU 37 37 -
GESVTIEDMATTTG MERB_BACSR 39 39 -
GRPVTVEQIAQATD MERB_STRLI 39 39 -
GGPVSREKLAGALG Q9F3W6 37 37 -
GDLYHAKSSPAQLG O07303 37 37 -
GRPISRTTLARTLG Q9F3W5 34 34 -
GRPVTTGGLATATG Q9L7K6 37 37 -
GQAVTESSLINQTG Q9WWL2 36 36 -

Motif 2 width=15
Element Seqn Id St Int Rpt
GLTLRETSYVFEIDD MERB_SERMA 75 27 -
GLTLRETSYVFEIDD P77072 75 27 -
GLTLRETSYVFEIDD Q9S4I1 75 27 -
GLTLRETSYVFEIDD Q9S4I2 75 27 -
GLTLRETSYVFEIDD Q9Z3Y8 75 27 -
GLTLFPTPHRFEVDG MERB_STAAU 78 27 -
GLTLIPTPHHFTVDG MERB_BACSR 80 27 -
GLTQNPTPHHFEVDG MERB_STRLI 80 27 -
GLTLRETSHVFEVDG Q9F3W6 78 27 -
GLNLRDTSHVFEVDG O07303 78 27 -
VLTLRETSHVLEIDN Q9F3W5 75 27 -
GLTLNPTPHRYETGG Q9L7K6 78 27 -
GLSIIPTTNQIHLGG Q9WWL2 81 31 -

Motif 3 width=10
Element Seqn Id St Int Rpt
LYAWCALDTL MERB_SERMA 92 2 -
LYAWCALDTL P77072 92 2 -
LYAWCALDTL Q9S4I1 92 2 -
LYAWCALDTL Q9S4I2 92 2 -
LYAWCALDTL Q9Z3Y8 92 2 -
LYAWCALDTL MERB_STAAU 95 2 -
LYAWCALDTL MERB_BACSR 97 2 -
LYTWCALDTL MERB_STRLI 97 2 -
LYTWCALDTL Q9F3W6 95 2 -
LYTWCVLDTL O07303 95 2 -
LYAWCALDTL Q9F3W5 92 2 -
LYTWCALDTL Q9L7K6 95 2 -
LFAWCAISTL Q9WWL2 98 2 -

Motif 4 width=12
Element Seqn Id St Int Rpt
CAATGAPVSLTV MERB_SERMA 117 15 -
CAATGAPVSLTV P77072 117 15 -
CAATGAPVSLTV Q9S4I1 117 15 -
CAATGAPVSLTV Q9S4I2 117 15 -
CAATGAPVSLTV Q9Z3Y8 117 15 -
CHGTGKSVRLTV MERB_STAAU 120 15 -
CHSTGEPIRLNV MERB_BACSR 122 15 -
CHATGTPVRLTV MERB_STRLI 122 15 -
CAATGRPITLTV Q9F3W6 120 15 -
CAATGRPITLTV O07303 119 14 -
CAATGAPVSLTV Q9F3W5 117 15 -
CHATGEPVRLTV Q9L7K6 120 15 -
CAYTGEPIEVTV Q9WWL2 123 15 -

Motif 5 width=16
Element Seqn Id St Int Rpt
HFFASVPTAEDWASKH MERB_SERMA 163 34 -
HFFASVPTAEDWASKH P77072 163 34 -
HFFASVPTAEDWASKH Q9S4I1 163 34 -
HFFASVPTAEDWASKH Q9S4I2 163 34 -
HFFASVPTAEDWASKH Q9Z3Y8 163 34 -
HFFSSPSAAQDWLNQH MERB_STAAU 166 34 -
HFFSSPNAAEDWLDQH MERB_BACSR 168 34 -
HFFATPDAGKGWLEEH MERB_STRLI 168 34 -
HFFASPSIANSWASTH Q9F3W6 166 34 -
HFFASPSIANSWASTH O07303 165 34 -
HFFASVPTAEDWASKH Q9F3W5 163 34 -
HFFATPDAGKDWLEEH Q9L7K6 166 34 -
HYFSSVEHANKWKEEH Q9WWL2 169 34 -