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PR01597

Identifier
ZONOCCLUDNS  [View Relations]  [View Alignment]  
Accession
PR01597
No. of Motifs
4
Creation Date
23-AUG-2001
Title
Zona occludens protein family signature
Database References
PRINTS; PR01598 ZONOCCLUDNS1; PR01599 ZONOCCLUDNS2; PR01600 ZONOCCLUDNS3

PRODOM; PD021419
Literature References
1. TSUKITA, S. AND FURUSE, M.
Overcoming barriers in the study of tight junction functions: from occludin
to claudin.
GENES CELLS 3 569-573 (1998).
 
2. TSUKITA, S. AND FURUSE, M.
Occludins and claudins in tight-junction strands: leading or supporting
players?
TRENDS CELL BIOL. 9 268-273 (1999).
 
3. ITOH, M., NAHAFUCHI, A., MOROI, S. AND TSUKITA, S.
Involvement of ZO-1 in cadherin-based cell adhesion through its direct 
binding to alpha catenin and actin filaments.
J.CELL BIOL. 138 181-192 (1997).
 
4. ITOH, M., FURUSE, M., MORITA, K., KUBOTA, K., SAITOU, M. AND TSUKITA S.
Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and 
ZO-3, with the COOH termini of claudins.
J.CELL BIOL. 147 1351-1363 (1999).
 
5. FANNING, A., JAMESON, B., JESAITIS, L. AND ANDERSON, J.
The tight junction protein ZO-1 establishes a link between the transmembrane
protein occludin and the actin cytoskeleton.
J.BIOL.CHEM. 273 29745-29753 (1998).

Documentation
Zona occludens (ZO), or tight junctions (TJ), are specialised membrane 
domains found at the most apical region of polarised epithelial and endo-
thelial cells that create a primary barrier, preventing paracellular 
transport of solutes, and restricting the lateral diffusion of membrane 
lipids and proteins, thus maintaining cellular polarity [1]. Under freeze-
fracture electron microscopy, TJs appear as a network of continuous 
anastomosing intramembranous strands. These strands consist mainly of 
claudins and occludin, which are transmembrane proteins that polymerise 
within plasma membranes to form fibrils [2].
 
The zona occludens proteins (ZO-1, ZO-2 and ZO-3) are a family of TJ- 
associated proteins that function as cross-linkers, anchoring the TJ strand 
proteins to the actin-based cytoskeleton [3,4]. Each protein contains three 
PDZ (postsynaptic density, disc-large, ZO-1) domains, a single SH3 (Src 
Homology-3) domain and a GK (guanylate kinase) domain, the presence of which
identifies them as members of the membrane-associated guanylate kinase 
(MAGUK) protein family. They also share an acidic domain at the C-terminal 
region of the molecules not found in other MAGUK proteins. It has been
demonstrated that the first PDZ domain is involved in binding the C-terminal
-Y-V motif of claudins [4]. By contrast, the occludin-binding domain of 
ZO-1 has been shown to lie in the GK and acidic domains [5]. Although the 
precise location of the actin-binding motif has not been elucidated, it 
appears to be within the C-terminal half of the molecules, since 
transfection of this region into fibroblasts induces co-localisation 
of ZO-1 and ZO-2 with actin fibres [5].
 
ZONOCCLUDNS is a 4-element fingerprint that provides a signature for the 
zona occludens protein family. The fingerprint was derived from an initial 
alignment of 10 sequences: the motifs were drawn from conserved regions 
spanning virtually the full alignment length - motif 1 lies between the 
first and second PDZ domain; motif 2 resides between the SH3 and the GK 
domain; and motif 3 lies beyond the GK domain in the C-terminal portion of 
the alignment. A single iteration on SPTR39_22_17.3f was required to reach 
convergence, no further sequences being identified beyond the starting set. 
Summary Information
10 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
410101010
30000
20000
1234
True Positives
O97758        Q9YHV2        ZO1_HUMAN     ZO1_MOUSE     
ZO2_CANFA ZO2_HUMAN ZO2_MOUSE ZO3_CANFA
ZO3_HUMAN ZO3_MOUSE
Sequence Titles
O97758      ZO-1 MDCK - Canis familiaris (Dog).           
Q9YHV2 TIGHT JUNCTION PROTEIN - Gallus gallus (Chicken).
ZO1_HUMAN TIGHT JUNCTION PROTEIN ZO-1 (ZONULA OCCLUDENS 1 PROTEIN) (ZONA OCCLUDENS 1 PROTEIN) (TIGHT JUNCTION PROTEIN 1) - Homo sapiens (Human).
ZO1_MOUSE TIGHT JUNCTION PROTEIN ZO-1 (ZONULA OCCLUDENS 1 PROTEIN) (ZONA OCCLUDENS 1 PROTEIN) (TIGHT JUNCTION PROTEIN 1) - Mus musculus (Mouse).
ZO2_CANFA TIGHT JUNCTION PROTEIN ZO-2 (ZONULA OCCLUDENS 2 PROTEIN) (ZONA OCCLUDENS 2 PROTEIN) (TIGHT JUNCTION PROTEIN 2) - Canis familiaris (Dog).
ZO2_HUMAN TIGHT JUNCTION PROTEIN ZO-2 (ZONULA OCCLUDENS 2 PROTEIN) (ZONA OCCLUDENS 2 PROTEIN) (TIGHT JUNCTION PROTEIN 2) - Homo sapiens (Human).
ZO2_MOUSE TIGHT JUNCTION PROTEIN ZO-2 (ZONULA OCCLUDENS 2 PROTEIN) (ZONA OCCLUDENS 2 PROTEIN) (TIGHT JUNCTION PROTEIN 2) - Mus musculus (Mouse).
ZO3_CANFA TIGHT JUNCTION PROTEIN ZO-3 (ZONULA OCCLUDENS 3 PROTEIN) (ZONA OCCLUDENS 3 PROTEIN) (TIGHT JUNCTION PROTEIN 3) - Canis familiaris (Dog).
ZO3_HUMAN TIGHT JUNCTION PROTEIN ZO-3 (ZONULA OCCLUDENS 3 PROTEIN) (ZONA OCCLUDENS 3 PROTEIN) (TIGHT JUNCTION PROTEIN 3) - Homo sapiens (Human).
ZO3_MOUSE TIGHT JUNCTION PROTEIN ZO-3 (ZONULA OCCLUDENS 3 PROTEIN) (ZONA OCCLUDENS 3 PROTEIN) (TIGHT JUNCTION PROTEIN 3) - Mus musculus (Mouse).
Scan History
SPTR39.22_17.3f 1  300  NSINGLE    
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
EKSRGKLQLVVLRDSKQTLINIP ZO2_CANFA 355 355 -
EKSRGKLQLVVLRDSQQTLINIP ZO2_HUMAN 371 371 -
ERSKGKLKMVVQRDERATLLNVP O97758 249 249 -
ERSKGKLKMVVQRDERATLLNVP ZO1_HUMAN 238 238 -
ERSKGKLKMVVQRDERATLLNVP ZO1_MOUSE 250 250 -
EKSRGKLQLVVLRDRKQTLLNIP Q9YHV2 338 338 -
EKSRGKLQLVVLRDSKQTLINIP ZO2_MOUSE 351 351 -
EKSEGKLSLLVLRDRGQFLVNIP ZO3_HUMAN 258 258 -
EKSEGKLTLLVLRDRGQFLVNIP ZO3_CANFA 250 250 -
EKSEGELTLLVLRDSGQFLVNIP ZO3_MOUSE 250 250 -

Motif 2 width=12
Element Seqn Id St Int Rpt
RADFWRMRGQRS ZO2_CANFA 666 288 -
RADFWRMRGQRS ZO2_HUMAN 682 288 -
RADFWRFRGLRS O97758 597 325 -
RADFWRFRGLRS ZO1_HUMAN 586 325 -
RADFWRFRGLRS ZO1_MOUSE 598 325 -
RADFWRTRGQRS Q9YHV2 651 290 -
RAVFWWMRRQRS ZO2_MOUSE 662 288 -
RAEFWRLRGLRR ZO3_HUMAN 585 304 -
RAEFWRLRGLRR ZO3_CANFA 560 287 -
RAEFWRLRSLRR ZO3_MOUSE 563 290 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SCDSRLISDFE ZO2_CANFA 897 219 -
SCDSRLISDFE ZO2_HUMAN 913 219 -
STDSRHTSDYE O97758 833 224 -
STDSRHTSDYE ZO1_HUMAN 822 224 -
STDSRHTSDYE ZO1_MOUSE 834 224 -
SCDSRLISDLE Q9YHV2 882 219 -
SCDSRLISDFE ZO2_MOUSE 895 221 -
SCDSRVNSDYE ZO3_HUMAN 812 215 -
SCDSRVNSDYE ZO3_CANFA 787 215 -
SCDSRTNSDYE ZO3_MOUSE 790 215 -

Motif 4 width=15
Element Seqn Id St Int Rpt
EEPLVSSITRSSEPV ZO2_CANFA 926 18 -
EEPLVSSITRSSEPV ZO2_HUMAN 942 18 -
GTPPESAITRSSEPV O97758 866 22 -
GTPPESAITRSSEPV ZO1_HUMAN 855 22 -
GTPPESAITRSSEPV ZO1_MOUSE 867 22 -
DEPRISSVGRSSEPV Q9YHV2 911 18 -
EEPLVSSITRSSEPV ZO2_MOUSE 924 18 -
DEPPAPALARSSEPV ZO3_HUMAN 849 26 -
EGPPAPALARSSEPV ZO3_CANFA 814 16 -
EEVAPTALARSSEPV ZO3_MOUSE 820 19 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
EKSRGKLQLVVLRDSKQTLINIP ZO2_CANFA 355 355 -
EKSRGKLQLVVLRDSQQTLINIP ZO2_HUMAN 371 371 -
ERSKGKLKMVVQRDERATLLNVP O97758 249 249 -
ERSKGKLKMVVQRDERATLLNVP ZO1_HUMAN 238 238 -
ERSKGKLKMVVQRDERATLLNVP ZO1_MOUSE 250 250 -
EKSRGKLQLVVLRDRKQTLLNIP Q9YHV2 338 338 -
EKSRGKLQLVVLRDSKQTLINIP ZO2_MOUSE 351 351 -
EKSEGKLSLLVLRDRGQFLVNIP ZO3_HUMAN 258 258 -
EKSEGKLTLLVLRDRGQFLVNIP ZO3_CANFA 250 250 -
EKSEGELTLLVLRDSGQFLVNIP ZO3_MOUSE 250 250 -

Motif 2 width=12
Element Seqn Id St Int Rpt
RADFWRMRGQRS ZO2_CANFA 666 288 -
RADFWRMRGQRS ZO2_HUMAN 682 288 -
RADFWRFRGLRS O97758 597 325 -
RADFWRFRGLRS ZO1_HUMAN 586 325 -
RADFWRFRGLRS ZO1_MOUSE 598 325 -
RADFWRTRGQRS Q9YHV2 651 290 -
RAVFWWMRRQRS ZO2_MOUSE 662 288 -
RAEFWRLRGLRR ZO3_HUMAN 585 304 -
RAEFWRLRGLRR ZO3_CANFA 560 287 -
RAEFWRLRSLRR ZO3_MOUSE 563 290 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SCDSRLISDFE ZO2_CANFA 897 219 -
SCDSRLISDFE ZO2_HUMAN 913 219 -
STDSRHTSDYE O97758 833 224 -
STDSRHTSDYE ZO1_HUMAN 822 224 -
STDSRHTSDYE ZO1_MOUSE 834 224 -
SCDSRLISDLE Q9YHV2 882 219 -
SCDSRLISDFE ZO2_MOUSE 895 221 -
SCDSRVNSDYE ZO3_HUMAN 812 215 -
SCDSRVNSDYE ZO3_CANFA 787 215 -
SCDSRTNSDYE ZO3_MOUSE 790 215 -

Motif 4 width=15
Element Seqn Id St Int Rpt
EEPLVSSITRSSEPV ZO2_CANFA 926 18 -
EEPLVSSITRSSEPV ZO2_HUMAN 942 18 -
GTPPESAITRSSEPV O97758 866 22 -
GTPPESAITRSSEPV ZO1_HUMAN 855 22 -
GTPPESAITRSSEPV ZO1_MOUSE 867 22 -
DEPRISSVGRSSEPV Q9YHV2 911 18 -
EEPLVSSITRSSEPV ZO2_MOUSE 924 18 -
DEPPAPALARSSEPV ZO3_HUMAN 849 26 -
EGPPAPALARSSEPV ZO3_CANFA 814 16 -
EEVAPTALARSSEPV ZO3_MOUSE 820 19 -