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PR01492

Identifier
KV6CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01492
No. of Motifs
5
Creation Date
15-MAR-2001
Title
Kv6 potassium channel alpha subunit signature
Database References
PRINTS; PR00169 KCHANNEL; PR01491 KVCHANNEL
Literature References
1. MILLER, C.
An overview of the potassium channel family.
GENOME BIOL.
2. ASHCROFT, F.M.
Voltage-gated K+ channels.
IN ION CHANNELS AND DISEASE, ACADEMIC PRESS, 2000, PP.97-123.
 
3. SANSOM, M.S.
Putting the parts together.
CURR.BIOL. 9(19) R738-R741 (1999).   
 
4. SALINAS, M., DUPRAT, F., HEURTEAUX, C., HUGNOT, J-P. AND LAZDUNSKI, M.
New modulatory alpha subunits for mammalian shab K+ channels.
J.BIOL.CHEM. 272(39) 24371-24379 (1997).
 
5. KRAMER, J.W., POST, M.A., BROWN, A.M. AND KIRSCH G.E.
Modulation of potassium channel gating by coexpression of Kv2.1 with
regulatory Kv5.1 or Kv6.1 alpha-subunits.
AM.J.PHYSIOL. 274 C1501-C1510 (1998).

Documentation
Potassium ion (K+) channels are a structurally diverse group of proteins
that facilitate the flow of K+ ions across cell membranes. They are
ubiquitous, being present in virtually all cell types. Activation of K+
channels tends to hyperpolarise cells, reducing the membrane's electrical
resistance, dampening nervous activity. In eukaryotic cells, K+ channels
are involved in neural signalling and generation of the cardiac rhythm, and
act as effectors in signal transduction pathways involving G protein-
coupled receptors (GPCRs). In prokaryotic cells, they play a role in the
maintenance of ionic homeostasis [1].
   
Structurally, Kv channels belong to the subfamily of K+ channels whose
subunits contain 6 transmembrane (TM) domains: these are the voltage-
gated K+ (Kv) channels, the KCNQ channels, the EAG-like K+ channels and
3 kinds of Ca2+-activated K+ channels (BK, IK and SK) [2]. All K+
channels share a characteristic sequence feature: a TMxTVGYG motif
that resides between the 2 C-terminal membrane spanning helices, and
forms the K+-selective pore domain [1-2].
 
The Kv family can be divided into 4 subfamilies on the basis of sequence
similarity and function: Shaker (Kv1), Shab (Kv2), Shaw (Kv3) and Shal 
(Kv4). All consist of pore-forming alpha subunits that associate with 
different types of beta subunit. To form a functional K+ channel pore,
4 alpha subunits and 4 beta subunits are required. The alpha subunits
have 6 well-conserved TM domains, a pore loop region and variable length
N- and C-termini. The TM domains 5/6 and the loop region form the K+ channel
pore through which the K+ ions pass [3]. 
 
More recently, 4 new electrically-silent alpha subunits have been cloned:
Kv5 (KCNF), Kv6 (KCNG), Kv8 and Kv9 (KCNS). These subunits do not themselves
possess any functional activity, but appear to form heteromeric channels
with Kv2 subunits, and thus modulate Shab channel activity [4]. When highly
expressed, they inhibit channel activity, but at lower levels show more
specific modulatory actions. Coexpression of Kv6 subunits with Shab subunits
has a slowing effect on Shab channel inactivation at strong depolarising
potentials, but has little effect on channel inactivation at intermediate
potentials [5].
 
KV6CHANNEL is a 5-element fingerprint that provides a signature for the Kv6
potassium channel alpha subunits. The fingerprint was derived from an
initial alignment of 3 sequences: the motifs were drawn from conserved 
regions spanning virtually the full alignment length - motifs 1-4 reside
within the N-terminal cytoplasmic domain; and motif 5 spans the C-terminus
of TM domain 6 leading into the C-terminal cytoplasmic domain. Two
iterations on SPTR39_15f were required to reach convergence, at which
point a true set comprising 5 sequences was identified.
Summary Information
5 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
555555
400000
300000
200000
12345
True Positives
O43528        O73606        Q9QYU3        Q9UIX4        
Q9UJ96
Sequence Titles
O43528      POTASSIUM CHANNEL - Homo sapiens (Human).     
O73606 SHAB-LIKE VOLTAGE-GATED POTASSIUM CHANNEL CKV6.2 - Gallus gallus (Chicken).
Q9QYU3 CARDIAC POTASSIUM CHANNEL SUBUNIT (KV6.2) - Rattus rattus (black rat).
Q9UIX4 DJ955M13.1 (POTASSIUM VOLTAGE-GATED CHANNEL, SUBFAMILY G, MEMBER 1) - Homo sapiens (Human).
Q9UJ96 CARDIAC POTASSIUM CHANNEL SUBUNIT (KV6.2) - Homo sapiens (Human).
Scan History
SPTR39_15f 2  8    NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
DDILNVCDDYDVT Q9UIX4 99 99 -
DELLRVCDDYDVS Q9QYU3 67 67 -
DEIMNICDDYDVS O73606 93 93 -

Motif 2 width=17
Element Seqn Id St Int Rpt
GKLRLLREMCALSFQEE Q9UIX4 134 22 -
GKLRLLRGPCALAFRDE Q9QYU3 102 22 -
GKLRLLREMCALSFQEE O73606 128 22 -

Motif 3 width=16
Element Seqn Id St Int Rpt
YWGIAEDHLDGCCKRR Q9UIX4 153 2 -
YWGIDEARLERCCLRR Q9QYU3 121 2 -
YWGIEEDHLEWCCKKR O73606 147 2 -

Motif 4 width=13
Element Seqn Id St Int Rpt
LGRCMRRLRDMVE Q9UIX4 206 37 -
LERGRRRLRDVVE Q9QYU3 169 32 -
LSLCMRNLRDMVE O73606 196 33 -

Motif 5 width=15
Element Seqn Id St Int Rpt
SRSYLELKQEQERVM Q9UIX4 464 245 -
SRSYSELKEQQQRAA Q9QYU3 422 240 -
SRSYSELKEQQQRAA O73606 457 248 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
DDILNVCDDYDVT Q9UIX4 99 99 -
DDILNVCDDYDVT O43528 99 99 -
DELLRVCDDYDVS Q9QYU3 67 67 -
DDLLRVCDDYDVS Q9UJ96 53 53 -
DEIMNICDDYDVS O73606 93 93 -

Motif 2 width=17
Element Seqn Id St Int Rpt
GKLRLLREMCALSFQEE Q9UIX4 134 22 -
GKLRLLREMCALSFQEE O43528 134 22 -
GKLRLLRGPCALAFRDE Q9QYU3 102 22 -
GKLRLLRGPCALAFRDE Q9UJ96 88 22 -
GKLRLLREMCALSFQEE O73606 128 22 -

Motif 3 width=16
Element Seqn Id St Int Rpt
YWGIAEDHLDGCCKRR Q9UIX4 153 2 -
YWGIAEDHLDGCCKRR O43528 153 2 -
YWGIDEARLERCCLRR Q9QYU3 121 2 -
YWGIDEARLERCCLRR Q9UJ96 107 2 -
YWGIEEDHLEWCCKKR O73606 147 2 -

Motif 4 width=13
Element Seqn Id St Int Rpt
LGRCMRRLRDMVE Q9UIX4 206 37 -
LGRCMRRLRDMVE O43528 206 37 -
LERGRRRLRDVVE Q9QYU3 169 32 -
LQRGRRRLRDVVD Q9UJ96 156 33 -
LSLCMRNLRDMVE O73606 196 33 -

Motif 5 width=15
Element Seqn Id St Int Rpt
SRSYLELKQEQERVM Q9UIX4 464 245 -
SPSYLELKQEQERVM O43528 464 245 -
SRSYSELKEQQQRAA Q9QYU3 422 240 -
SRSYSELKEQQQRAA Q9UJ96 409 240 -
SRSYSELKEQQQRAA O73606 457 248 -