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PR01449

Identifier
BKCHANNELA  [View Relations]  [View Alignment]  
Accession
PR01449
No. of Motifs
8
Creation Date
05-DEC-2000
Title
Calcium-activated BK potassium channel alpha subunit signature
Database References
PRINTS; PR00169 KCHANNEL
Literature References
1. MILLER, C.
An overview of the potassium channel family.
GENOME BIOL. 1(4) 1-5 (2000).
 
2. ASHCROFT, F.M.
Ca2+ activated K+ channels.
IN ION CHANNELS AND DISEASE, ACADEMIC PRESS, 2000, PP.125-133.
 
3. VERGARA, C., LATORRE, R., MARRION, N.V. AND ADELMAN, J.P.
Calcium-activated potassium channels.
CURR.OPIN.NEUROBIOL. 8(3) 321-329 (1998).

Documentation
Potassium ion (K+) channels are a structurally diverse group of proteins
that facilitate the flow of K+ ions across cell membranes. They are
ubiquitous, being present in virtually all cell types. Activation of K+
channels tends to hyperpolarise cells, reducing the membrane's electrical
resistance, dampening nervous activity. In eukaryotic cells, K+ channels
are involved in neural signalling and generation of the cardiac rhythm, and
act as effectors in signal transduction pathways involving G protein-
coupled receptors (GPCRs). In prokaryotic cells, they play a role in the
maintenance of ionic homeostasis [1].
 
Structurally, calcium (Ca2+) activated channels belong to the subfamily of
K+ channels whose subunits contain 6 transmembrane (TM) domains: these are
the voltage-gated K+ (Kv) channels, the KCNQ channels, the EAG-like K+ 
channels and 3 kinds of Ca2+-activated K+ channel (BK, IK and SK) [2]. All
K+ channels share a characteristic sequence feature: a TMxTVGYG motif that
resides between the 2 C-terminal membrane-spanning helices, and forms
the K+-selective pore domain [1].
 
Ca2+-activated K+ channels are a diverse group of channels that are
activated by an increase in intracellular Ca2+ concentration. They are
found in the majority of nerve cells, where they modulate cell excitability
and action potential. Three types of Ca2+-activated K+ channel have been
characterised, termed small-conductance (SK), intermediate conductance (IK)
and large conductance (BK) respectively [2-3].
 
BK channels (also referred to as maxi-K channels) are widely expressed 
in the body, being found in glandular tissue, smooth and skeletal muscle, 
as well as in neural tissues. They have been demonstrated to regulate 
arteriolar and airway diameter, and also neurotransmitter release. Each
channel complex is thought to be composed of 2 types of subunit: the pore-
forming (alpha) subunits and smaller accessory (beta) subunits.
 
The alpha subunit of the BK channel was initially thought to share the
characteristic 6TM organisation of the voltage-gated K+ channels. However,
the molecule is now thought to possess an additional TM domain, with an 
extracellular N-terminus and intracellular C-terminus. This C-terminal 
region contains 4 predominantly hydrophobic domains, which are also thought
to lie intracellularly. The extracellular N-terminus and the first TM region
are required for modulation by the beta subunit. The precise location of the
Ca2+-binding site that modulates channel activation remains unknown, but it
is thought to lie within the C-terminal hydrophobic domains.
 
BKCHANNELA is an 8-element fingerprint that provides a signature for the
calcium-activated BK potassium channel alpha subunit. The fingerprint was
derived from an initial alignment of 2 sequences: the motifs were drawn
from conserved regions spanning the full alignment length, focusing on those
sections that characterise the BK channel alpha subunits but distinguish 
them from other members of the K+ channel superfamily. Two iterations on 
SPTR39_14f were required to reach convergence, at which point a true set 
comprising 24 sequences was identified. Several partial matches were also
found, all of which are members of the calcium-dependent BK potassium
channel family that match only 4 or 5 motifs.
Summary Information
  24 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
3 codes involving 5 elements
2 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
82424242424242424
700000000
600000000
533033030
422021010
300000000
200000000
12345678
True Positives
O12942        O18866        O18867        O46371        
O55180 O73728 O73729 O73730
O73731 O93569 Q08460 Q12917
Q12921 Q12960 Q13150 Q28265
Q62976 Q98951 Q9R196 Q9TT88
Q9UBB0 Q9UQK6 Q9W7J2 Q9WUI3
True Positive Partials
Codes involving 5 elements
Q24369 Q9TWA1 Q9VC51
Codes involving 4 elements
O54982 Q9U268
Sequence Titles
O12942      CALCIUM-ACTIVATED POTASSIUM CHANNEL - Gallus gallus (Chicken). 
O18866 CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA SUBUNIT - Sus scrofa (Pig).
O18867 CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA SUBUNIT - Macaca mulatta (Rhesus macaque).
O46371 LARGE CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA SUBUNIT - Oryctolagus cuniculus (Rabbit).
O55180 RAT-SLOWPOKE-ALPHA - Rattus norvegicus (Rat).
O73728 CALCIUM-ACTIVATED POTASSIUM CHANNEL - Trachemys scripta (Red-eared slider turtle).
O73729 CALCIUM-ACTIVATED POTASSIUM CHANNEL - Trachemys scripta (Red-eared slider turtle).
O73730 CALCIUM-ACTIVATED POTASSIUM CHANNEL - Trachemys scripta (Red-eared slider turtle).
O73731 CALCIUM-ACTIVATED POTASSIUM CHANNEL - Trachemys scripta (Red-eared slider turtle).
O93569 CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA SUBUNIT - Gallus gallus (Chicken).
Q08460 SLOWPOKE HOMOLOG, POTASSIUM CHANNEL, CALCIUM-ACTIVATED (CALCIUM-ACTIVATED POTASSIUM CHANNEL) (SLOWPOKE HOMOLOG PROTEIN) - Mus musculus (Mouse).
Q12917 CALCIUM DEPENDENT POTASSIUM CHANNEL ALPHA SUBUNIT - Homo sapiens (Human).
Q12921 CALCIUM ACTIVATED POTASSIUM CHANNEL - Homo sapiens (Human).
Q12960 LARGE-CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNEL - Homo sapiens (Human).
Q13150 CALCIUM-ACTIVATED POTASSIUM CHANNEL - Homo sapiens (Human).
Q28265 CALCIUM ACTIVATED POTASSIUM CHANNEL PROTEIN - Canis familiaris (Dog).
Q62976 CALCIUM ACTIVATED POTASSIUM CHANNEL - Rattus norvegicus (Rat).
Q98951 CALCIUM-ACTIVATED POTASSIUM CHANNEL - Gallus gallus (Chicken).
Q9R196 LARGE CONDUCTANCE CALCIUM ACTIVATED POTASSIUM BK CHANNEL STREX-1 VARIANT - Mus musculus (Mouse).
Q9TT88 MAXI-K CHANNEL ALPHA SUBUNIT - Oryctolagus cuniculus (Rabbit).
Q9UBB0 CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA SUBUNIT - Homo sapiens (Human).
Q9UQK6 BKCA ALPHA SUBUNIT - Homo sapiens (Human).
Q9W7J2 CALCIUM-ACTIVATED POTASSIUM CHANNEL ISOFORM THC7 - Trachemys scripta (Red-eared slider turtle) (Pseudemys scripta).
Q9WUI3 LARGE-CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNEL - Rattus norvegicus (Rat).

Q24369 CALCIUM ACTIVATED POTASSIUM CHANNEL - Drosophila melanogaster (Fruit fly).
Q9TWA1 CALCIUM-ACTIVATED POTASSIUM CHANNEL - Drosophila melanogaster (Fruit fly).
Q9VC51 SLO PROTEIN - Drosophila melanogaster (Fruit fly).

O54982 POTASSIUM LARGE CONDUCTANCE PH-SENSITIVE CHANNEL, SUBFAMILY M, ALPHA MEMBER 3 (PH SENSITIVE MAXI K+ CHANNEL) - Mus musculus (Mouse).
Q9U268 Y51A2D.19 PROTEIN - Caenorhabditis elegans.
Scan History
SPTR39_14f 2  40   NSINGLE    
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
EIVFLHNISPNLELEALFKRH Q12960 439 439 -
EIVFLHNISPNLELEALFKRH Q08460 401 401 -

Motif 2 width=21
Element Seqn Id St Int Rpt
ESADACLILANKYCADPDAED Q12960 482 22 -
ESADACLILANKYCADPDAED Q08460 444 22 -

Motif 3 width=14
Element Seqn Id St Int Rpt
YHPKIRIITQMLQY Q12960 515 12 -
YHPKIRIITQMLQY Q08460 477 12 -

Motif 4 width=16
Element Seqn Id St Int Rpt
QSCLAQGLSTMLANLF Q12960 561 32 -
QSCLAQGLSTMLANLF Q08460 523 32 -

Motif 5 width=18
Element Seqn Id St Int Rpt
FVGLSFPTVCELCFVKLK Q12960 610 33 -
FVGLSFPTVCELCFVKLK Q08460 572 33 -

Motif 6 width=15
Element Seqn Id St Int Rpt
SPKKKQRNGGMRNSP Q12960 707 79 -
SPKKKQRNGGMRNSP Q08460 672 82 -

Motif 7 width=16
Element Seqn Id St Int Rpt
FLDQDDDDDPDTELYL Q12960 955 233 -
FLDQDDDDDPDTELYL Q08460 920 233 -

Motif 8 width=16
Element Seqn Id St Int Rpt
SHSSHSSQSSSKKSSS Q12960 1132 161 -
SHSSHSSQSSSKKSSS Q08460 1097 161 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
EIVFLHNISPNLELEALFKRH O18866 413 413 -
EIVFLHNISPNLELEALFKRH Q9WUI3 440 440 -
EIVFLHNISPNLELEALFKRH Q9UBB0 415 415 -
EIVFLHNISPNLELEALFKRH Q9TT88 374 374 -
EIVFLHNISPNLELEALFKRH Q9R196 374 374 -
EIVFLHNISPNLELEALFKRH Q62976 409 409 -
EIVFLHNISPNLELEALFKRH Q13150 439 439 -
EIVFLHNISPNLELEALFKRH Q12960 439 439 -
EIVFLHNISPNLELEALFKRH Q12921 415 415 -
EIVFLHNISPNLELEALFKRH Q12917 374 374 -
EIVFLHNISPNLELEALFKRH Q08460 401 401 -
EIVFLHNISPNLELEALFKRH O55180 440 440 -
EIVFLHNISPNLELEALFKRH O46371 374 374 -
EIVFLHNISPNLELEALFKRH O18867 412 412 -
EIVFLHNISPDLELEALFKRH Q9UQK6 374 374 -
EIVFLHNISPNLELEALFKRH Q28265 374 374 -
EIVFLHNISPNLELEALFKRH O93569 398 398 -
EIVFLHNISPNLELEALFKRH Q98951 375 375 -
EIVFLHNISPDLELEALFKRH O73728 375 375 -
EIVFLHNISPDLELEALFKRH O73729 375 375 -
EIVFLHNISPDLELEALFKRH O73730 375 375 -
EIVFLHNISPDLELEALFKRH O73731 375 375 -
EIVFLHNISPDLELEALFKRH Q9W7J2 375 375 -
EIVFLHNISPNLELEALFKRH O12942 398 398 -

Motif 2 width=21
Element Seqn Id St Int Rpt
ESADACLILANKYCADPDAED O18866 456 22 -
ESADACLILANKYCADPDAED Q9WUI3 483 22 -
ESADACLILANKYCADPDAED Q9UBB0 458 22 -
ESADACLILANKYCADPDAED Q9TT88 417 22 -
ESADACLILANKYCADPDAED Q9R196 417 22 -
ESADACLILANKYCADPDAED Q62976 452 22 -
ESADACLILANKYCADPDAED Q13150 482 22 -
ESADACLILANKYCADPDAED Q12960 482 22 -
ESADACLILANKYCADPDAED Q12921 458 22 -
ESADACLILANKYCADPDAED Q12917 417 22 -
ESADACLILANKYCADPDAED Q08460 444 22 -
ESADACLILANKYCADPDAED O55180 483 22 -
ESADACLILANKYCADPDAED O46371 417 22 -
ESADACLILANKYCADPDAED O18867 455 22 -
ESADACLILANKYCADPDAED Q9UQK6 417 22 -
ESADACLILANKYCDDPDAED Q28265 417 22 -
ESADACLILANKYCADPDAED O93569 441 22 -
ESADACLILANKYCADPDAED Q98951 418 22 -
ESADACLILANKYCADPDAED O73728 418 22 -
ESADACLILANKYCADPDAED O73729 418 22 -
ESADACLILANKYCADPDAED O73730 418 22 -
ESADACLILANKYCADPDAED O73731 418 22 -
ESADACLILANKYCADPDAED Q9W7J2 418 22 -
ESADACLILANKYCADPDAED O12942 441 22 -

Motif 3 width=14
Element Seqn Id St Int Rpt
YHPKIRIITQMLQY O18866 489 12 -
YHPKIRIITQMLQY Q9WUI3 516 12 -
YHPKIRIITQMLQY Q9UBB0 491 12 -
YHPKIRIITQMLQY Q9TT88 450 12 -
YHPKIRIITQMLQY Q9R196 450 12 -
YHPKIRIITQMLQY Q62976 485 12 -
YHPKIRIITQMLQY Q13150 515 12 -
YHPKIRIITQMLQY Q12960 515 12 -
YHPKIRIITQMLQY Q12921 491 12 -
YHPKIRIITQMLQY Q12917 450 12 -
YHPKIRIITQMLQY Q08460 477 12 -
YHPKIRIITQMLQY O55180 516 12 -
YHPKIRIITQMLQY O46371 450 12 -
YHPKIRIITQMLQY O18867 488 12 -
YHPKIRIITQMLQY Q9UQK6 450 12 -
YHPKIRIITQMLQY Q28265 450 12 -
YHPKIRIITQMLQY O93569 474 12 -
YHPKIRIITQMLQY Q98951 451 12 -
YHPKIRIITQMLQY O73728 451 12 -
YHPKIRIITQMLQY O73729 451 12 -
YHPKIRIITQMLQY O73730 451 12 -
YHPKIRIITQMLQY O73731 451 12 -
YHPKIRIITQMLQY Q9W7J2 451 12 -
YHPKIRIITQMLQY O12942 474 12 -

Motif 4 width=16
Element Seqn Id St Int Rpt
QSCLAQGLSTMLANLF O18866 535 32 -
QSCLAQGLSTMLANLF Q9WUI3 562 32 -
QSCLAQGLSTMLANLF Q9UBB0 537 32 -
QSCLAQGLSTMLANLF Q9TT88 496 32 -
QSCLAQGLSTMLANLF Q9R196 496 32 -
QSCLAQGLSTMLANLF Q62976 531 32 -
QSCLAQGLSTMLANLF Q13150 561 32 -
QSCLAQGLSTMLANLF Q12960 561 32 -
QSCLAQGLSTMLANLF Q12921 537 32 -
QSCLAQGLSTMLANLF Q12917 496 32 -
QSCLAQGLSTMLANLF Q08460 523 32 -
QSCLAQGLSTMLANLF O55180 562 32 -
QSCLAQGLSTMLANLF O46371 496 32 -
QSCLAQGLSTMLANLF O18867 534 32 -
QSCLAQGLSTMLANLF Q9UQK6 496 32 -
QSCLAQGLSTMLANLF Q28265 496 32 -
QSCLAPGLSTMLANLF O93569 520 32 -
QSCLAPGLSTMLANLF Q98951 497 32 -
QSCLAPGLSTMLANLF O73728 497 32 -
QSCLAPGLSTMLANLF O73729 497 32 -
QSCLAPGLSTMLANLF O73730 497 32 -
QSCLAPGLSTMLANLF O73731 497 32 -
QSCLAPGLSTMLANLF Q9W7J2 497 32 -
QSSLAPGLSTMLANLF O12942 520 32 -

Motif 5 width=18
Element Seqn Id St Int Rpt
FVGLSFPTVCELCFVKLK O18866 584 33 -
FVGLSFPTVCELCFVKLK Q9WUI3 611 33 -
FVGLSFPTVCELCFVKLK Q9UBB0 586 33 -
FVGLSFPTVCELCFVKLK Q9TT88 545 33 -
FVGLSFPTVCELCFVKLK Q9R196 545 33 -
FVGLSFPTVCELCFVKLK Q62976 580 33 -
FVGLSFPTVCELCFVKLK Q13150 610 33 -
FVGLSFPTVCELCFVKLK Q12960 610 33 -
FVGLSFPTVCELCFVKLK Q12921 586 33 -
FVGLSFPTVCELCFVKLK Q12917 545 33 -
FVGLSFPTVCELCFVKLK Q08460 572 33 -
FVGLSFPTVCELCFVKLK O55180 611 33 -
FVGLSFPTVCELCFVKLK O46371 545 33 -
FVGLSFPTVCELCFVKLK O18867 583 33 -
FVGLSFPTVCELCFVKLK Q9UQK6 545 33 -
FVGLSFPTVCELCFVKLK Q28265 545 33 -
FVGLSFPAVCELVFAKLK O93569 569 33 -
FVGLSFPAVCELVFAKLK Q98951 546 33 -
FVGLSFPAVCELVFAKLK O73728 546 33 -
FVGLSFPAVCELVFAKLK O73729 546 33 -
FVGLSFPAVCELVFAKLK O73730 546 33 -
FVGLSFPAVCELVFAKLK O73731 546 33 -
FVGLSFPAVCELVFAKLK Q9W7J2 546 33 -
FVGLSFPAVCELVFAKLK O12942 569 33 -

Motif 6 width=15
Element Seqn Id St Int Rpt
SPKKKQRNGGMRNSP O18866 681 79 -
SPKKKQRNGGMRNSP Q9WUI3 712 83 -
SPKKKQRNGGMRNSP Q9UBB0 683 79 -
SPKKKQRNGGMRNSP Q9TT88 700 137 -
SPKKKQRNGGMRNSP Q9R196 700 137 -
SPKKKQRNGGMRNSP Q62976 677 79 -
SPKKKQRNGGMRNSP Q13150 707 79 -
SPKKKQRNGGMRNSP Q12960 707 79 -
SPKKKQRNGGMRNSP Q12921 683 79 -
SPKKKQRNGGMRNSP Q12917 642 79 -
SPKKKQRNGGMRNSP Q08460 672 82 -
SPKKKQRNGGMRNSP O55180 773 144 -
SPKKKQRNGGMRNSP O46371 642 79 -
SPKKKQRNGGMRNSP O18867 680 79 -
SPKKKQRNGGMRNSP Q9UQK6 642 79 -
SPKKKQRNGGMRNSP Q28265 642 79 -
SPKKKQRNGGMRNSP O93569 666 79 -
SPKKKQRNGGMRNSP Q98951 647 83 -
SPKKKQRNGGMRNSP O73728 643 79 -
SPKKKQRNGGMRNSP O73729 650 86 -
SPKKKQRNGGMRNSP O73730 708 144 -
SPKKKQRNGGMRNSP O73731 677 113 -
SPKKKQRNGGMRNSP Q9W7J2 621 57 -
SPKKKQRNGGMRNSP O12942 666 79 -

Motif 7 width=16
Element Seqn Id St Int Rpt
FLDQDDDDDPDTELYL O18866 929 233 -
FLDQDDDDDPDTELYL Q9WUI3 987 260 -
FLDQDDDDDPDTELYL Q9UBB0 931 233 -
FLDQDDDDDPDTELYL Q9TT88 948 233 -
FLDQDDDDDPDTELYL Q9R196 948 233 -
FLDQDDDDDPDTELYL Q62976 925 233 -
FLDQDDDDDPDTELYL Q13150 955 233 -
FLDQDDDDDPDTELYL Q12960 955 233 -
FLDQDDDDDPDTELYL Q12921 931 233 -
FLDQDDDDDPDTELYL Q12917 890 233 -
FLDQDDDDDPDTELYL Q08460 920 233 -
FLDQDDDDDPDTELYL O55180 1021 233 -
FLDQDDDDDPDTELYL O46371 890 233 -
FLDQDDDDDPDTELYL O18867 928 233 -
FLDQDDDDDPDTELYL Q9UQK6 890 233 -
FLDQDDDDDPDTELYL Q28265 890 233 -
FLDQDDDDDPDTELYL O93569 914 233 -
FLDQDDDDDPDTELYL Q98951 895 233 -
FLDQDDDDDPDTELYL O73728 891 233 -
FLDQDDDDDPDTELYL O73729 898 233 -
FLDQDDDDDPDTELYL O73730 956 233 -
FLDQDDDDDPDTELYL O73731 925 233 -
FLDQDDDDDPDTELYL Q9W7J2 869 233 -
FLDQDDDDDPDTELYL O12942 914 233 -

Motif 8 width=16
Element Seqn Id St Int Rpt
SHSSHSSQSSSKKSSS O18866 1106 161 -
SHSSHSSQSSSKKSSS Q9WUI3 1164 161 -
SHSSHSSQSSSKKSSS Q9UBB0 1108 161 -
SHSSHSSQSSSKKSSS Q9TT88 1125 161 -
SHSSHSSQSSSKKSSS Q9R196 1125 161 -
SHSSHSSQSSSKKSSS Q62976 1102 161 -
SHSSHSSQSSSKKSSS Q13150 1132 161 -
SHSSHSSQSSSKKSSS Q12960 1132 161 -
SHSSHSSQSSSKKSSS Q12921 1108 161 -
SHSSHSSQSSSKKSSS Q12917 1067 161 -
SHSSHSSQSSSKKSSS Q08460 1097 161 -
SHSSHSSQSSSKKSSS O55180 1198 161 -
SHSSHSSQSSSKKSSS O46371 1067 161 -
SHSSHSSQSSSKKSSS O18867 1105 161 -
SHSSHSSQSSSKKSSS Q9UQK6 1067 161 -
SHSSHSSQSSSKKSSS Q28265 1067 161 -
SHSSHSSYSSSKKSSS O93569 1091 161 -
SHSSHSSYSSSKKSSS Q98951 1072 161 -
SHSSHSSYSSSKKSSS O73728 1068 161 -
SHSSHSSYSSSKKSSS O73729 1075 161 -
SHSSHSSYSSSKKSSS O73730 1133 161 -
SHSSHSSYSSSKKSSS O73731 1102 161 -
SHSSHSSYSSSKKSSS Q9W7J2 1046 161 -
SHSSHSSYSSSKKSSS O12942 1091 161 -