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PR01389

Identifier
CDTOXINC  [View Relations]  [View Alignment]  
Accession
PR01389
No. of Motifs
5
Creation Date
07-JUN-2000
Title
Cytolethal distending toxin C signature
Database References
Literature References
1. PICKETT, C.L., COTTLE, D.L., PESCI, E.C. AND BIKAH, G.
Cloning, sequencing, and expression of the Escherichia coli cytolethal
distending toxin genes.
INFECT.IMMUN. 62 1046-1051 (1994).
 
2. EYIGOR, I., DAWSON, K.A., LANGLOIS, B.E. AND PICKETT, C.L.
Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter 
coli isolates; detection and analysis by PCR.
J.CLIN.MICROBIOL. 37 1646-1650 (1999).
 
3. DE RYCKE, J., SERT, V., COMAYRAS, C. AND TASCA, C.
Sequence of lethal events in HeLa cells exposed to the G2 blocking 
cytolethal distending toxin.
EUR.J.CELL BIOL. 29 192-201 (2000).

Documentation
Escherichia coli, Haemophilus spp. and Campylobacter spp. all produce 
a toxin that is seen to cause distension in certain cell lines [1,2], 
which eventually disintegrate and die. This novel toxin, termed cytolethal 
distending toxin (cdt), has three subunits: A, B, and C. Their sizes are 
approx. 27.7, 29.5 and 19.9kDa respectively [1], and they appear to be 
entirely novel [2]. 
 
Further research on the complete toxin has revealed that it blocks the cell
cycle at stage G2, through inactivation of the cyclin-dependent kinase Cdk1
[3], and without induction of DNA breaks. This leads to multipolar abortive 
mitosis and micronucleation, associated with centrosomal amplification [3].
The roles of each subunit are unclear, but it is believed that they have
separate roles in pathogenicity.
 
CDTOXINC is a 5-element fingerprint that provides a signature for cytolethal
distending toxin C proteins. The fingerprint was derived from an initial 
alignment of 2 sequences: the motifs were drawn from conserved regions 
spanning the full alignment length (~160 amino acids). Two iterations on
SPTR37_10f were required to reach convergence, at which point a true set
comprising 3 sequences was identified. 
Summary Information
3 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
533333
400000
300000
200000
12345
True Positives
O32587        Q46670        Q47090        
Sequence Titles
O32587      CYTOLETHAL DISTENDING TOXIN-IIIC - ESCHERICHIA COLI. 
Q46670 CDTC - ESCHERICHIA COLI.
Q47090 CDTC - ESCHERICHIA COLI.
Scan History
SPTR37_10f 2  25   NSINGLE    
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
FTMLLIAGCSSSQDSANN Q46670 8 8 -
FFVLLLTGCASEPANQRN Q47090 7 7 -

Motif 2 width=16
Element Seqn Id St Int Rpt
SGLMIHNGLHQHGRET Q46670 45 19 -
TGGIIRNPVGSDFNVN Q47090 52 27 -

Motif 3 width=17
Element Seqn Id St Int Rpt
RTPNTDQCLGTPDGRNL Q46670 86 25 -
KNPDGNECFAILNGQLA Q47090 92 24 -

Motif 4 width=19
Element Seqn Id St Int Rpt
TCNSTAKKTLFSLIPSTTG Q46670 106 3 -
QCSESDRNALFTFITSDTG Q47090 112 3 -

Motif 5 width=18
Element Seqn Id St Int Rpt
CIADFKKPFEVVPQSHLW Q46670 152 27 -
CVDDLGRPFDTVPPGLLW Q47090 157 26 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
FTMLLIAGCSSSQDSANN Q46670 8 8 -
VTMLLIAGCSSSQEAVNN O32587 8 8 -
FFVLLLTGCASEPANQRN Q47090 7 7 -

Motif 2 width=16
Element Seqn Id St Int Rpt
SGLMIHNGLHQHGRET Q46670 45 19 -
SGLMLHNRLDLHGREI O32587 45 19 -
TGGIIRNPVGSDFNVN Q47090 52 27 -

Motif 3 width=17
Element Seqn Id St Int Rpt
RTPNTDQCLGTPDGRNL Q46670 86 25 -
RTPNTDQCLGTPDGKNL O32587 86 25 -
KNPDGNECFAILNGQLA Q47090 92 24 -

Motif 4 width=19
Element Seqn Id St Int Rpt
TCNSTAKKTLFSLIPSTTG Q46670 106 3 -
TCNPTDKKTLFSLIPSTTG O32587 106 3 -
QCSESDRNALFTFITSDTG Q47090 112 3 -

Motif 5 width=18
Element Seqn Id St Int Rpt
CIADFKKPFEVVPQSHLW Q46670 152 27 -
CITDLKQPFKVVPQSHLW O32587 152 27 -
CVDDLGRPFDTVPPGLLW Q47090 157 26 -