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PR01365

Identifier
TELOMERASERT  [View Relations]  [View Alignment]  
Accession
PR01365
No. of Motifs
4
Creation Date
17-AUG-2000
Title
Telomere reverse transcriptase signature
Database References
Literature References
1. LINGNER, J., HUGHES, T.R., SCHEVCHENKO, A., MANN, M., LUNDBLAD, V. AND
CECH, T.R.
Reverse transcriptase motifs in the catalytic subunit of telomerase.
SCIENCE 276 561-567 (2000).
 
2. LIU, J-P.
Telomerase: Not just Black and White, but Shades of Gray.
MOL.CELL.BIOL.RES.COMMUN. 3 129-135 (2000).
 
3. WANG, J., HANNON, G.J. AND BEACH, D.H.
Risky immortalization by telomerase.
NATURE 405 755-756 (2000).

Documentation
Telomerase is an enzyme that, in certain cells, maintains the physical ends
of chromosomes (telomeres) during replication. In somatic cells, replication
of the lagging strand requires the continual presence of an RNA primer
approximately 200 nucleotides upstream, which is complementary to the
template strand. Since there is a region of DNA less than 200 base pairs
from the end of the chromosome where this is not possible, the chromosome is
continually shortened. However, there is a surplus of repetitive DNA at the 
ends, the telomeres, that protects against the erosion of gene-encoding DNA.
 
Telomerase is a ribonucleoprotein (RNP) that synthesises the telomeric DNA
repeats. The telomerase RNA subunit provides the template for synthesis of
these repeats. There are 2 protein components of the RNP: the catalytic
subunit is known as telomerase reverse transcriptase (TERT). The reverse
transcriptase domain is located in the C-terminal region of the TERT
polypeptide and single amino acid substitutions in this region lead to
telomere shortening and senescence [1].
 
Telomerase is not normally expressed in somatic cells and it has been
suggested that exogenous TERT may extend the lifespan of, or even
immortalise, the cell. However, recent studies have shown that telomerase
activity can be induced by a number of oncogenes [2]. Conversely, the
oncogene c-myc can be activated in human TERT immortalised cells [3].
 
TELOMERASERT is a 4-element fingerprint that provides a signature for the
telomere reverse transcriptase. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved regions
spanning the C-terminal half of the alignment. Two iterations on SPTR37_10f
were required to reach convergence, at which point a true set comprising 9
sequences was identified.
Summary Information
9 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
49999
30000
20000
1234
True Positives
O00939        O13338        O13339        O14746        
O14783 O70372 O76332 O77448
Q06163
Sequence Titles
O00939      TELOMERASE SUBUNIT P123 - EUPLOTES AEDICULATUS. 
O13338 TELOMERASE REVERSE TRANSCRIPTASE 1 - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
O13339 TELOMERASE REVERSE TRANSCRIPTASE 1 (EC 2.7.7.-) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
O14746 TELOMERASE REVERSE TRANSCRIPTASE - HOMO SAPIENS (HUMAN).
O14783 TELOMERASE CATALYTIC SUBUNIT - HOMO SAPIENS (HUMAN).
O70372 TELOMERASE REVERSE TRANSCRIPTASE - MUS MUSCULUS (MOUSE).
O76332 TELOMERASE REVERSE TRANSCIPTASE - OXYTRICHA TRIFALLAX.
O77448 TELOMERASE REVERSE TRANSCRIPTASE - TETRAHYMENA THERMOPHILA.
Q06163 CHROMOSOME XII COSMID 8543 - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Scan History
SPTR37_10f 2  30   NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
FYVTETTFQKNRLFF O14746 561 561 -
FYITESTFQKNRLFF O70372 551 551 -
FYITESSDLRNRTVY O13339 444 444 -
FYSTEKAKEYQRIFY O76332 560 560 -
FYCTEISSTVTIVYF Q06163 381 381 -

Motif 2 width=17
Element Seqn Id St Int Rpt
PELYFVKVDVTGAYDTI O14746 704 128 -
PRMYFVKADVTGAYDAI O70372 694 128 -
RKKYFVRIDIKSCYDRI O13339 582 123 -
PKLYFVAMDIEKCYDNV O76332 700 125 -
PELYFMKFDVKSCYDSI Q06163 522 126 -

Motif 3 width=17
Element Seqn Id St Int Rpt
YVQCQGIPQGSILSTLL O14746 825 104 -
YTQCQGIPQGSSLSTLL O70372 818 107 -
YLQKVGIPQGSILSSFL O13339 698 99 -
YKQMKGIPQGLCVSYIL O76332 834 117 -
YIREDGLFQGSSLSAPI Q06163 624 85 -

Motif 4 width=13
Element Seqn Id St Int Rpt
LLLRLVDDFLLVT O14746 862 20 -
LLLRFVDDFLLVT O70372 855 20 -
VLLRVVDDFLFIT O13339 736 21 -
LLMRLTDDYLLMT O76332 880 29 -
LILKLADDFLIIS Q06163 664 23 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
FYVTETTFQKNRLFF O14746 561 561 -
FYVTETTFQKNRLFF O14783 561 561 -
FYITESTFQKNRLFF O70372 551 551 -
FYITESSDLRNRTVY O13338 444 444 -
FYITESSDLRNRTVY O13339 444 444 -
FYSTEKAKEYQRIFY O76332 560 560 -
FYVTEQQKSYSKTYY O00939 456 456 -
FYITEKHKEGSQIFY O77448 476 476 -
FYCTEISSTVTIVYF Q06163 381 381 -

Motif 2 width=17
Element Seqn Id St Int Rpt
PELYFVKVDVTGAYDTI O14746 704 128 -
PELYFVKVDVTGAYDTI O14783 704 128 -
PRMYFVKADVTGAYDAI O70372 694 128 -
RKKYFVRIDIKSCYDRI O13338 583 124 -
RKKYFVRIDIKSCYDRI O13339 582 123 -
PKLYFVAMDIEKCYDNV O76332 700 125 -
PKLFFATMDIEKCYDSV O00939 595 124 -
PQLYYVTLDIKKCYDSI O77448 610 119 -
PELYFMKFDVKSCYDSI Q06163 522 126 -

Motif 3 width=17
Element Seqn Id St Int Rpt
YVQCQGIPQGSILSTLL O14746 825 104 -
YVQCQGIPQGSILSTLL O14783 825 104 -
YTQCQGIPQGSSLSTLL O70372 818 107 -
YLQKVGIPQGSILSSFL O13338 699 99 -
YLQKVGIPQGSILSSFL O13339 698 99 -
YKQMKGIPQGLCVSYIL O76332 834 117 -
YKQTKGIPQGLCVSSIL O00939 729 117 -
FRQKRGIPQGLNISGVL O77448 765 138 -
YIREDGLFQGSSLSAPI Q06163 624 85 -

Motif 4 width=13
Element Seqn Id St Int Rpt
LLLRLVDDFLLVT O14746 862 20 -
LLLRLVDDFLLVT O14783 862 20 -
LLLRFVDDFLLVT O70372 855 20 -
VLLRVVDDFLFIT O13338 737 21 -
VLLRVVDDFLFIT O13339 736 21 -
LLMRLTDDYLLMT O76332 880 29 -
LLMRLTDDYLLIT O00939 775 29 -
LLMRLTDDYLFIS O77448 809 27 -
LILKLADDFLIIS Q06163 664 23 -