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PR01329

Identifier
KIR33CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01329
No. of Motifs
5
Creation Date
12-MAY-2000
Title
Kir3.3 inward rectifier K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01320 KIRCHANNEL
PRODOM; PD151575
Literature References
1. MINOR, D.L., JR., MASSELLING, S.J., JAN, Y.N. AND JAN, L.Y.
Transmembrane structure of an inwardly rectifying potassium channel.
CELL 96 879-891 (1999).
 
2. DOUPNIK, C.A., DAVIDSON, N. AND LESTER, H.A.
The inward rectifier potassium channel family.
CURR.OPIN.NEUROBIOL. 5 268-277 (1995).
 
3. REIMANN, F. AND ASHCROFT, F.M.
Inwardly rectifying potassium channels.
CURR.OPIN.CELL BIOL. 11 503-508 (1999).

Documentation
Potassium channels are found in virtually all cell types. Their pore-
forming subunits fall into three structural families, i.e. those possessing
six, four and two transmembrane (TM) domains. The six-TM domain K+ channels
can be further subdivided into six families: the voltage-gated K+ channels
(Kv), the KCNQ channels, the eag-like K+ channels, and three Ca2+-activated
K+ channels. Inwardly-rectifying K+ channels (Kir) are the principal class
of two-TM domain K+ channels, and the recently-discovered two-pore domain
K+ channels, make up a family of four-TM domain K+ channels.
 
Inwardly rectifying potassium channels (Kir) are responsible for regulating
diverse processes including: cellular excitability, vascular tone, heart
rate, renal salt flow, and insulin release [1]. To date, around twenty
members of this superfamily have been cloned, which can be grouped into six
families by sequence similarity, and these are designated Kir1.x-7.x [2,3].
 
Cloned Kir channel cDNAs encode proteins of between ~370-500 residues,
containing two predicted TM domains, with the characteristic K+ channel
pore-forming domain located between them. Both N- and C-termini are thought
to be cytoplasmic, and the N-terminus lacks a signal sequence. It is thought
that four Kir subunits assemble to form a tetrameric channel complex, which
may be hetero- or homomeric [1].
 
The Kir3.x channel family are gated by G proteins following G-protein
coupled receptor (GPCR) activation. They are widely distributed in
neuronal, atrial, and endocrine tissues and play key roles in generating
late inhibitory postsynaptic potentials, slowing the heart rate and
modulating hormone release. They are directly activated by G protein
beta-gamma subunits released from G protein heterotrimers of the G(i/o)
family upon appropriate receptor stimulation.
 
Kir3.3 does not generate receptor-evoked, or constitutively-active K+
currents, when heterologously expressed in Xenopus oocytes. It may
therefore contribute to Kir channel diversity by associating with other
Kir3.x family members [2].
 
KIR33CHANNEL is a 5-element fingerprint that provides a signature for the
Kir3.3 inward rectifier K+ channel. The fingerprint was derived from an
initial alignment of 3 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise Kir3.3 but distinguish it from other subtypes -
motif 1 encodes the first 15 residues of the putative cytoplasmic
N-terminus; motif 2 spans the end of the N-terminal cytoplasmic domain and
the start of the following first TM domain; and motifs 3-5 encode portions
of the cytoplasmic C-terminus. A single iteration on SPTR37_10f was
required to reach convergence, no further sequences being identified beyond
the starting set.
Summary Information
3 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
533333
400000
300000
200000
12345
True Positives
IRK9_HUMAN    IRK9_MOUSE    IRK9_RAT      
Sequence Titles
IRK9_HUMAN  G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 3 (GIRK3) (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 9) (KIR3.3) - HOMO SAPIENS (HUMAN). 
IRK9_MOUSE G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 3 (GIRK3) (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 9) (KIR3.3) - MUS MUSCULUS (MOUSE).
IRK9_RAT G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 3 (GIRK3) (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 9) (KIR3.3) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 1  5    NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
MAQENAAFSPGSEEP IRK9_RAT 1 1 -
MAQENAAFSPGQEEP IRK9_HUMAN 1 1 -
MAQENAAFSPGSEEP IRK9_MOUSE 1 1 -

Motif 2 width=15
Element Seqn Id St Int Rpt
QWRLSLLFFVLAYAL IRK9_RAT 56 40 -
QWRLSLLFFVLAYAL IRK9_HUMAN 56 40 -
QWRLRLLFFVLAYAL IRK9_MOUSE 56 40 -

Motif 3 width=10
Element Seqn Id St Int Rpt
EASRRALERD IRK9_RAT 259 188 -
EASRRALERD IRK9_HUMAN 259 188 -
EASRRALERD IRK9_MOUSE 259 188 -

Motif 4 width=10
Element Seqn Id St Int Rpt
AAARLDAHLY IRK9_RAT 341 72 -
AAARLDAHLY IRK9_HUMAN 341 72 -
AAARLDAHLY IRK9_MOUSE 341 72 -

Motif 5 width=13
Element Seqn Id St Int Rpt
IPSRLDEKVEEEG IRK9_RAT 353 2 -
IPSRLDEKVEEEG IRK9_HUMAN 353 2 -
IPSRLDEKVEEEG IRK9_MOUSE 353 2 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
MAQENAAFSPGSEEP IRK9_RAT 1 1 -
MAQENAAFSPGQEEP IRK9_HUMAN 1 1 -
MAQENAAFSPGSEEP IRK9_MOUSE 1 1 -

Motif 2 width=15
Element Seqn Id St Int Rpt
QWRLSLLFFVLAYAL IRK9_RAT 56 40 -
QWRLSLLFFVLAYAL IRK9_HUMAN 56 40 -
QWRLRLLFFVLAYAL IRK9_MOUSE 56 40 -

Motif 3 width=10
Element Seqn Id St Int Rpt
EASRRALERD IRK9_RAT 259 188 -
EASRRALERD IRK9_HUMAN 259 188 -
EASRRALERD IRK9_MOUSE 259 188 -

Motif 4 width=10
Element Seqn Id St Int Rpt
AAARLDAHLY IRK9_RAT 341 72 -
AAARLDAHLY IRK9_HUMAN 341 72 -
AAARLDAHLY IRK9_MOUSE 341 72 -

Motif 5 width=13
Element Seqn Id St Int Rpt
IPSRLDEKVEEEG IRK9_RAT 353 2 -
IPSRLDEKVEEEG IRK9_HUMAN 353 2 -
IPSRLDEKVEEEG IRK9_MOUSE 353 2 -