SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01309

Identifier
P2X2RECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01309
No. of Motifs
10
Creation Date
23-MAR-2000
Title
P2X2 purinoceptor signature
Database References
PRINTS; PR00252 NRIONCHANNEL; PR01307 P2XRECEPTOR
PRODOM; PD041642
Literature References
1. NORTH, R.A.
Families of ion channels with two hydrophobic segments.
CURR.OPIN.CELL BIOL. 8 474-483 (1996).
 
2. MACKENZIE, A.B., SURPRENANT, A. AND NORTH, R.A.
Functional and molecular diversity of purinergic ion channel receptors.
ANN.N.Y.ACAD.SCI. 868 716-729 (1999).

Documentation
P2X purinoceptors are cell membrane ion channels, gated by adenosine
5'-triphosphate (ATP) and other nucleotides. They have been found to be
widely expressed on mammalian cells, and, by means of their functional 
properties, can be differentiated into three sub-groups. The first group is
almost equally well activated by ATP and its analogue alphabetamethyleneATP,
whereas, the second group is not activated by the latter compound. A third
type of receptor (also called P2Z) is distinguished by the fact that
repeated or prolonged agonist application leads to the opening of much
larger pores, allowing large molecules to traverse the cell membrane. This
increased permeability rapidly leads to cell death, and lysis.
 
Molecular cloning studies have identified seven P2X receptor subtypes,
designated P2X1-P2X7. These receptors are proteins that share 35-48% amino
acid identity, and possess two putative transmembrane (TM) domains,
separated by a long (~270 residues) intervening sequence, which is
thought to form an extracellular loop. Around 1/4 of the residues within
the loop are invariant between the cloned subtypes, including 10
characteristic cysteines.
 
Studies of the functional properties of heterologously expressed P2X
receptors, together with the examination of their distribution in native
tissues, suggests they likely occur as both homo- and heteromultimers
in vivo [1,2].
 
P2X2 receptors (which have been found to be alternatively spliced), are
half-maximally activated by a concentration of ATP of ~10 micromolar. 
In contrast, alphabetamethyleneATP is found to be largely ineffective. This
agonist profile has been found to be shared by the P2X4, P2X5 and P2X6
receptors. The single-channel properties of the P2X2 receptor are quite
similar to those noted for the native receptor present on PC12 cells [2].
 
P2X2RECEPTOR is a 10-element fingerprint that provides a signature for the
P2X purinoceptor isoform 2. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections
that characterise the P2X purinoceptor isoform 2 but distinguish it from
others - motif 1 resides within the putative cytoplasmic N-terminus; motifs
2-8 lie within the exoplasmic loop connecting the two putative TM domains;
and motifs 9-10 encode portions of the putative cytoplasmic C-terminus. Two
iterations on SPTR37_10f were required to reach convergence, at which point
a true set comprising 4 sequences was identified.
Summary Information
4 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
104444444444
90000000000
80000000000
70000000000
60000000000
50000000000
40000000000
30000000000
20000000000
12345678910
True Positives
O54869        O70399        P2X2_CAVPO    P2X2_RAT      
Sequence Titles
O54869      P2X PURINOCEPTOR 2 (ATP RECEPTOR) (P2X2) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT). 
O70399 P2X2 RECEPTOR SPLICE VARIANT P2X2-3 - CAVIA PORCELLUS (GUINEA PIG).
P2X2_CAVPO P2X PURINOCEPTOR 2 (ATP RECEPTOR) (P2X2) (PURINERGIC RECEPTOR) - CAVIA PORCELLUS (GUINEA PIG).
P2X2_RAT P2X PURINOCEPTOR 2 (ATP RECEPTOR) (P2X2) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 2  50   NSINGLE    
Initial Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
RQGWSAFWD O70399 15 15 -
RQGWSAFWD P2X2_CAVPO 15 15 -

Motif 2 width=12
Element Seqn Id St Int Rpt
DSETGPESSIIT O70399 65 41 -
DSETGPESSIIT P2X2_CAVPO 65 41 -

Motif 3 width=19
Element Seqn Id St Int Rpt
ITQSEHKVWDVEEYVKPPE O70399 81 4 -
ITQSEHKVWDVEEYVKPPE P2X2_CAVPO 81 4 -

Motif 4 width=11
Element Seqn Id St Int Rpt
SIRVPNTTCHL O70399 124 24 -
SIRVPNTTCHL P2X2_CAVPO 124 24 -

Motif 5 width=11
Element Seqn Id St Int Rpt
CTAGELDMLGN O70399 138 3 -
CTAGELDMLGN P2X2_CAVPO 138 3 -

Motif 6 width=9
Element Seqn Id St Int Rpt
YYHGEAKTC O70399 158 9 -
YYHGEAKTC P2X2_CAVPO 158 9 -

Motif 7 width=10
Element Seqn Id St Int Rpt
SHFLGKMAPN O70399 181 14 -
SHFLGKMAPN P2X2_CAVPO 181 14 -

Motif 8 width=16
Element Seqn Id St Int Rpt
FIVEQAGENFTELAHR O70399 239 48 -
FIVEQAGENFTELAHR P2X2_CAVPO 239 48 -

Motif 9 width=20
Element Seqn Id St Int Rpt
WILLTFMNKNKVYSHKKFDK O70399 384 129 -
WILLTFMNKNKVYSHKKFDK P2X2_CAVPO 357 102 -

Motif 10 width=14
Element Seqn Id St Int Rpt
QDSTPTDPKGLAQL O70399 488 84 -
QDSTPTDPKGLAQL P2X2_CAVPO 461 84 -
Final Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
RQGWSAFWD O70399 15 15 -
RQGWSAFWD P2X2_CAVPO 15 15 -
RGCWSAFWD P2X2_RAT 7 7 -
RGCWSAFWD O54869 7 7 -

Motif 2 width=12
Element Seqn Id St Int Rpt
DSETGPESSIIT O70399 65 41 -
DSETGPESSIIT P2X2_CAVPO 65 41 -
DSETGPESSIIT P2X2_RAT 57 41 -
DSETGPESSIIT O54869 69 53 -

Motif 3 width=19
Element Seqn Id St Int Rpt
ITQSEHKVWDVEEYVKPPE O70399 81 4 -
ITQSEHKVWDVEEYVKPPE P2X2_CAVPO 81 4 -
ITMSEDKVWDVEEYVKPPE P2X2_RAT 73 4 -
ITMSEDKVWDVEEYVKPPE O54869 85 4 -

Motif 4 width=11
Element Seqn Id St Int Rpt
SIRVPNTTCHL O70399 124 24 -
SIRVPNTTCHL P2X2_CAVPO 124 24 -
SMRVHSSTCHS P2X2_RAT 116 24 -
SMRVHSSTCHS O54869 128 24 -

Motif 5 width=11
Element Seqn Id St Int Rpt
CTAGELDMLGN O70399 138 3 -
CTAGELDMLGN P2X2_CAVPO 138 3 -
CIAGQLDMQGN P2X2_RAT 130 3 -
CIAGQLDMQGN O54869 142 3 -

Motif 6 width=9
Element Seqn Id St Int Rpt
YYHGEAKTC O70399 158 9 -
YYHGEAKTC P2X2_CAVPO 158 9 -
YYHGDSKTC P2X2_RAT 150 9 -
YYHGDSRTC O54869 162 9 -

Motif 7 width=10
Element Seqn Id St Int Rpt
SHFLGKMAPN O70399 181 14 -
SHFLGKMAPN P2X2_CAVPO 181 14 -
NHFLGKMAPN P2X2_RAT 173 14 -
NHFLGKMAPN O54869 185 14 -

Motif 8 width=16
Element Seqn Id St Int Rpt
FIVEQAGENFTELAHR O70399 239 48 -
FIVEQAGENFTELAHR P2X2_CAVPO 239 48 -
FIVEKAGENFTELAHK P2X2_RAT 231 48 -
FIVEKAGENFTELAHK O54869 243 48 -

Motif 9 width=20
Element Seqn Id St Int Rpt
WILLTFMNKNKVYSHKKFDK O70399 384 129 -
WILLTFMNKNKVYSHKKFDK P2X2_CAVPO 357 102 -
WILLTFMNKNKLYSHKKFDK P2X2_RAT 350 103 -
WILLTFMNKNKLYSHKKFDK O54869 362 103 -

Motif 10 width=14
Element Seqn Id St Int Rpt
QDSTPTDPKGLAQL O70399 488 84 -
QDSTPTDPKGLAQL P2X2_CAVPO 461 84 -
QDSTSTDPKGLAQL P2X2_RAT 459 89 -
QDSTSTDPKGLAQL O54869 471 89 -