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PR01014

Identifier
NRPEPTIDEY2R  [View Relations]  [View Alignment]  
Accession
PR01014
No. of Motifs
11
Creation Date
30-NOV-1998  (UPDATE 07-JUN-1999)
Title
Neuropeptide Y2 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01012 NRPEPTIDEYR
INTERPRO; IPR001358
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Neuropeptide Y.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.194-198.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Neuropeptide Y (NPY) is one of the most abundant peptides in mammalian
brain, inducing a variety of behavioural effects (e.g., stimulation of food
intake, anxiety, facilitation of learning and memory, and regulation of the
cardiovascular and neuroendocrine systems) [6]. In the periphery, NPY 
stimulates vascular smooth muscle contraction and modulates hormone
secretion. NPY has been implicated in the pathophysiology of hypertension, 
congestive heart failure, affective disorders and appetite regulation [6].
 
Several pharmacologically distinct neuropeptide Y receptors have been 
characterised, designated NPY Y1-Y6. High densities of Y2 receptors are 
present in rat hippocampus and are also found in high levels in superficial
layers of cortex, certain thalamic nuclei, lateral septum, and anterior 
olfactory nuclei; lower levels are found in striatum [6]. The receptors are
found in high levels in smooth muscle (e.g., vas deferens and intestine), 
kidney proximal tubules and in cell lines [6]. They are believed to have a
predominantly presynaptic location, and are involved in inhibition of
adenylyl cyclase and voltage dependent calcium channels via a pertussis-
toxin-sensitive G protein, probably of the G0/Gi class [6]. 
 
NRPEPTIDEY2R is an 11-element fingerprint that provides a signature for
neuropeptide Y2 receptors. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the Y2 receptors but distinguish them from the rest of
the neuropeptide Y family - motifs 1-3 span the N-terminus, leading into
TM domain 1; motifs 4 and 5 span the C-terminus of TM domain 4 and the 
second external loop; motifs 6 and 7 span the C-terminus of TM domain 5 
and the third cytoplasmic loop; motif 8 spans the C-terminus of TM domain 6 
and the third external loop; and motifs 9-11 reside at the C-terminus. Two 
iterations on OWL30.2 were required to reach convergence, at which point 
a true set comprising 5 sequences was identified. Two partial matches were 
also found: OAU83458 is an ovine neuropeptide Y2 receptor fragment that 
matches motifs 4-6; and AF054870 is a rat neuropeptide Y2 receptor fragment
that matches motifs 5 and 6.
 
An update on SPTR37_9f identified a true set of 4 sequences.
Summary Information
4 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
1144444444444
1000000000000
900000000000
800000000000
700000000000
600000000000
500000000000
400000000000
300000000000
200000000000
1234567891011
True Positives
NY2R_BOVIN    NY2R_HUMAN    NY2R_MOUSE    NY2R_PIG      
Sequence Titles
NY2R_BOVIN  NEUROPEPTIDE Y RECEPTOR TYPE 2 (NPY2-R) - BOS TAURUS (BOVINE). 
NY2R_HUMAN NEUROPEPTIDE Y RECEPTOR TYPE 2 (NPY2-R) - HOMO SAPIENS (HUMAN).
NY2R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 2 (NPY2-R) - MUS MUSCULUS (MOUSE).
NY2R_PIG NEUROPEPTIDE Y RECEPTOR TYPE 2 (NPY2-R) - SUS SCROFA (PIG).
Scan History
OWL30_2    2  50   NSINGLE    
SPTR37_9f 2 5 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
GPIGAEADENQTVEEMKV NY2R_HUMAN 2 2 -
GPLGAEADENQTVEEMKV NY2R_BOVIN 4 4 -

Motif 2 width=17
Element Seqn Id St Int Rpt
TTPRGELVPDPEPELID NY2R_HUMAN 26 6 -
TTLPGELAPDSEPELID NY2R_BOVIN 29 7 -

Motif 3 width=18
Element Seqn Id St Int Rpt
DSTKLIEVQVVLILAYCS NY2R_HUMAN 42 -1 -
DSTKLIEVQVVLILAYCS NY2R_BOVIN 45 -1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
LASPLAIFREYSLI NY2R_HUMAN 179 119 -
LASPLAIFREYSLI NY2R_BOVIN 182 119 -

Motif 5 width=16
Element Seqn Id St Int Rpt
EIIPDFEIVACTEKWP NY2R_HUMAN 193 0 -
EIIPDFEIVACTEKWP NY2R_BOVIN 196 0 -

Motif 6 width=17
Element Seqn Id St Int Rpt
GIISFSYTRIWSKLKNH NY2R_HUMAN 233 24 -
GIISFSYTRIWSKLKNH NY2R_BOVIN 236 24 -

Motif 7 width=17
Element Seqn Id St Int Rpt
VSPGAANDHYHQRRQKT NY2R_HUMAN 250 0 -
VSPGAAHDHYHQRRQKT NY2R_BOVIN 253 0 -

Motif 8 width=19
Element Seqn Id St Int Rpt
FQLAVDIDSQVLDLKEYKL NY2R_HUMAN 287 20 -
FQLAVDIDSHVLDLKEYKL NY2R_BOVIN 290 20 -

Motif 9 width=17
Element Seqn Id St Int Rpt
AFLSAFRCEQRLDAIHS NY2R_HUMAN 335 29 -
AFLSAFRCEQRLDAIHS NY2R_BOVIN 338 29 -

Motif 10 width=14
Element Seqn Id St Int Rpt
EVSVTFKAKKNLEV NY2R_HUMAN 352 0 -
EVSVTFKAKKHLQV NY2R_BOVIN 355 0 -

Motif 11 width=16
Element Seqn Id St Int Rpt
VRKNSGPNDSFTEATN NY2R_HUMAN 365 -1 -
VTKNNGPNDSFTETTN NY2R_BOVIN 368 -1 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
GPIGAEADENQTVEEMKV NY2R_HUMAN 2 2 -
GPLGAEADENQTVEEMKV NY2R_BOVIN 4 4 -
GPVGAEADENQTVEVKVE NY2R_MOUSE 6 6 -
GPIGAEADENQTVEEMKM NY2R_PIG 2 2 -

Motif 2 width=17
Element Seqn Id St Int Rpt
TTPRGELVPDPEPELID NY2R_HUMAN 26 6 -
TTLPGELAPDSEPELID NY2R_BOVIN 29 7 -
TTPRGELPPDPEPELID NY2R_MOUSE 30 6 -
TTPRGELAPDSEPELKD NY2R_PIG 27 7 -

Motif 3 width=18
Element Seqn Id St Int Rpt
DSTKLIEVQVVLILAYCS NY2R_HUMAN 42 -1 -
DSTKLIEVQVVLILAYCS NY2R_BOVIN 45 -1 -
DSTKLVEVQVILILAYCS NY2R_MOUSE 46 -1 -
DSTKLIEVQIILILAYCS NY2R_PIG 43 -1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
LASPLAIFREYSLI NY2R_HUMAN 179 119 -
LASPLAIFREYSLI NY2R_BOVIN 182 119 -
LASPLAIFREYSLI NY2R_MOUSE 183 119 -
LASPLAIFREYSLI NY2R_PIG 180 119 -

Motif 5 width=16
Element Seqn Id St Int Rpt
EIIPDFEIVACTEKWP NY2R_HUMAN 193 0 -
EIIPDFEIVACTEKWP NY2R_BOVIN 196 0 -
EIIPDFEIVACTEKWP NY2R_MOUSE 197 0 -
EIIPDFEIVACTEKKW NY2R_PIG 194 0 -

Motif 6 width=17
Element Seqn Id St Int Rpt
GIISFSYTRIWSKLKNH NY2R_HUMAN 233 24 -
GIISFSYTRIWSKLKNH NY2R_BOVIN 236 24 -
GIISFSYTRIWSKLRNH NY2R_MOUSE 237 24 -
GIISFSYARIWSKLKNH NY2R_PIG 235 25 -

Motif 7 width=17
Element Seqn Id St Int Rpt
VSPGAANDHYHQRRQKT NY2R_HUMAN 250 0 -
VSPGAAHDHYHQRRQKT NY2R_BOVIN 253 0 -
VSPGAASDHYHQRRHKM NY2R_MOUSE 254 0 -
VSPGGVNDHYHQRRQKT NY2R_PIG 252 0 -

Motif 8 width=19
Element Seqn Id St Int Rpt
FQLAVDIDSQVLDLKEYKL NY2R_HUMAN 287 20 -
FQLAVDIDSHVLDLKEYKL NY2R_BOVIN 290 20 -
FQLAVDIDSHVLDLKEYKL NY2R_MOUSE 291 20 -
FQLAVDIDSQVLDLKEYKL NY2R_PIG 289 20 -

Motif 9 width=17
Element Seqn Id St Int Rpt
AFLSAFRCEQRLDAIHS NY2R_HUMAN 335 29 -
AFLSAFRCEQRLDAIHS NY2R_BOVIN 338 29 -
AFLSAFRCEQRLDAIHS NY2R_MOUSE 339 29 -
AFLSAFRCEQRLDAIHS NY2R_PIG 337 29 -

Motif 10 width=14
Element Seqn Id St Int Rpt
EVSVTFKAKKNLEV NY2R_HUMAN 352 0 -
EVSVTFKAKKHLQV NY2R_BOVIN 355 0 -
EVSMTFKAKKNLEV NY2R_MOUSE 356 0 -
EVSMTSKAKKNLEA NY2R_PIG 354 0 -

Motif 11 width=16
Element Seqn Id St Int Rpt
VRKNSGPNDSFTEATN NY2R_HUMAN 365 -1 -
VTKNNGPNDSFTETTN NY2R_BOVIN 368 -1 -
VKKNNGPTDSFSEATN NY2R_MOUSE 369 -1 -
ATKNGGPDDSFTEATN NY2R_PIG 367 -1 -