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PR00797

Identifier
STREPTOPAIN  [View Relations]  [View Alignment]  
Accession
PR00797
No. of Motifs
6
Creation Date
23-JUN-1997  (UPDATE 07-JUN-1999)
Title
Streptopain (C10) cysteine protease family signature
Database References

INTERPRO; IPR000200
Literature References
1. RAWLINGS, N.D. AND BARRETT, A.J.
Families of cysteine peptidases.
METHODS ENZYMOL. 244 461-486 (1994).
 
2. TAI, J.Y., KORTT, A.A., LIU, T.Y. AND ELLIOTT, S.D.
Primary structure of streptococcal proteinase III. Isolation of the 
cyanogen bromide peptides: complete covalent structure of the polypeptide
chain.
J.BIOL.CHEM. 251 1955-1959 (1976).
 
3. KORTT, A.A. AND LIU, T.Y.
On the mechanism of action of streptococcal proteinase II. Comparison of
the kinetics of proteinase- and papain-catalyzed hydrolysis of N-acylamino
acid esters.
BIOCHEMISTRY 12 328-337 (1973).

Documentation
Cysteine protease activity is dependent on an active dyad of cysteine and
histidine, the order and spacing of these residues varying in the 20 or so
known families. Families C1, C2 and C10 are loosely termed papain-like.  
Nearly half of all cysteine proteases are found exclusively in viruses [1].
 
Streptopain is a cysteine protease found in Streptococcus pyogenes that
shows some structural and functional similarity to papain (family C1) [1,2].
The order of the catalytic cysteine/histidine dyad is the same and the
surrounding sequences are similar. The two proteins also show similar
specificities, both preferring a hydrophobic residue at the P2 site [1,3].
Streptopain shows a high degree of sequence similarity to the Streptomyces
pyogenes exotoxin B, and strong similarity to the prtT gene product of
Porphyromonas gingivalis, both of which have been included in the family [1].
 
STREPTOPAIN is a 6-element fingerprint that provides a signature for the
streptopain (C10) family of cysteine proteases. The fingerprint was derived
from an initial alignment of 3 sequences: the motifs were drawn from 
conserved regions spanning virtually the full alignment length - motif 1
contains the active cysteine, and motif 4 the active histidine. Two
iterations on OWL29.3 were required to reach convergence, at which point
a true set comprising 10 sequences was identified.
 
An update on SPTR37_9f identified a true set of 17 sequences.
Summary Information
17 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6171717171717
5000000
4000000
3000000
2000000
123456
True Positives
PRTT_PORGI    Q54960        Q54961        Q54962        
Q54963 Q54964 Q54965 Q54966
Q54967 Q54968 Q57024 Q57082
Q57202 Q57211 Q57212 SPEB_STRPY
STCP_STRPY
Sequence Titles
PRTT_PORGI  THIOL PROTEASE/HEMAGGLUTININ PRTT PRECURSOR (EC 3.4.22.-) - PORPHYROMONAS GINGIVALIS (BACTEROIDES GINGIVALIS). 
Q54960 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q54961 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q54962 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q54963 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q54964 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q54965 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q54966 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q54967 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q54968 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q57024 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q57082 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q57202 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q57211 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
Q57212 PYROGENIC EXOTOXIN B - STREPTOCOCCUS PYOGENES.
SPEB_STRPY EXOTOXIN TYPE B PRECURSOR (SPE B) - STREPTOCOCCUS PYOGENES.
STCP_STRPY STREPTOPAIN PRECURSOR (EC 3.4.22.10) (STREPTOCOCCAL CYSTEINE PROTEINASE) (STREPTOCOCCUS PEPTIDASE A) (SPP) - STREPTOCOCCUS PYOGENES.
Scan History
OWL29_3    2  100  NSINGLE    
SPTR37_9f 2 19 NSINGLE
Initial Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
GQHAATGCVATATAQIMKYHNYPNK SPEB_STRPY 185 185 -
GQAATGHCVATATAQIMKYHNYPDK STCP_STRPY 40 40 -
GQQAYTGCVATAMGQIMRHYKWPEK PRTT_PORGI 177 177 -

Motif 2 width=17
Element Seqn Id St Int Rpt
AISELMADVGISVDMDY SPEB_STRPY 260 50 -
AISELMADVGISVDMDY STCP_STRPY 115 50 -
ALSTFMRDVSFSVNMQF PRTT_PORGI 247 45 -

Motif 3 width=20
Element Seqn Id St Int Rpt
VQRALKENFGYNQSVHQINR SPEB_STRPY 288 11 -
VQRALKENFGYNQSVHQINR STCP_STRPY 143 11 -
VERALRETFHYKKSLRYIHR PRTT_PORGI 275 11 -

Motif 4 width=25
Element Seqn Id St Int Rpt
KELSQNQPVYYQGVGKVGGHAFVID SPEB_STRPY 321 13 -
KELSQNQPVYYEGVGKVGGHAFVID STCP_STRPY 176 13 -
KELAENRPVYYAGADGSMGHAFVCD PRTT_PORGI 308 13 -

Motif 5 width=22
Element Seqn Id St Int Rpt
YHVNWGWGGVSDGFFRLDALNP SPEB_STRPY 353 7 -
YHVDWGWGGVSDGFFRLDALNP STCP_STRPY 208 7 -
FHFNWGWGGMSNGNFYLNLLNP PRTT_PORGI 340 7 -

Motif 6 width=22
Element Seqn Id St Int Rpt
SALGTGGGAGGFNGYQSAVVGI SPEB_STRPY 375 0 -
SALGTGGGAGGFNGYESAVVGI STCP_STRPY 230 0 -
GSLGTGAGDGGYSTDQEVVIGI PRTT_PORGI 362 0 -
Final Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
GQHAATGCVATATAQIMKYHNYPNK Q54962 185 185 -
GQHAATGCVATATAQIMKYHNYPNK SPEB_STRPY 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q57212 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q57211 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q57202 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q57082 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q57024 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q54968 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q54967 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q54964 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q54963 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q54965 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q54966 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q54960 185 185 -
GQHAATGCVATATAQIMKYHNYPNK Q54961 185 185 -
GQAATGHCVATATAQIMKYHNYPDK STCP_STRPY 40 40 -
GQQAYTGCVATAMGQIMRHYKWPEK PRTT_PORGI 177 177 -

Motif 2 width=17
Element Seqn Id St Int Rpt
AISELMADVGISVDMDY Q54962 260 50 -
AISELMADVGISVDMDY SPEB_STRPY 260 50 -
AISELMADVGISVDMDY Q57212 260 50 -
AISELMADVGISVDMDY Q57211 260 50 -
AISELMADVGISVDMDY Q57202 260 50 -
AISELMADVGISVDMDY Q57082 260 50 -
AISELMADVGISVDMDY Q57024 260 50 -
AISELMADVGISVDMDY Q54968 260 50 -
AISELMADVGISVDMDY Q54967 260 50 -
AISELMADVGISVDMDY Q54964 260 50 -
AISELMADVGISVDMDY Q54963 260 50 -
AISELMADVGISVDMDY Q54965 260 50 -
AISELMADVGISVDMDY Q54966 260 50 -
AISELMADVGISVDMDY Q54960 260 50 -
AISELMADVGISVDMDY Q54961 260 50 -
AISELMADVGISVDMDY STCP_STRPY 115 50 -
ALSTFMRDVSFSVNMQF PRTT_PORGI 247 45 -

Motif 3 width=20
Element Seqn Id St Int Rpt
VQRALKENFGYNQSVHQINR Q54962 288 11 -
VQRALKENFGYNQSVHQINR SPEB_STRPY 288 11 -
VQRALKENFGYNQSVHQINR Q57212 288 11 -
VQRALKENFGYNQSVHQINR Q57211 288 11 -
VQRALKENFGYNQSVHQINR Q57202 288 11 -
VQRALKENFGYNQSVHQINR Q57082 288 11 -
VQRALKENFGYNQSVHQINR Q57024 288 11 -
VQRALKENFGYNQSVHQINR Q54968 288 11 -
VQRALKENFGYNQSVHQINR Q54967 288 11 -
VQRALKENFGYNQSVHQINR Q54964 288 11 -
VQRALKENFGYNQSVHQINR Q54963 288 11 -
VQRALKENFGYNQSVHQINR Q54965 288 11 -
VQRALRENFGYNQSVHQINR Q54966 288 11 -
VQRALKENFGYNQSVHQINR Q54960 288 11 -
VQRALKENFGYNQSVHQVNR Q54961 288 11 -
VQRALKENFGYNQSVHQINR STCP_STRPY 143 11 -
VERALRETFHYKKSLRYIHR PRTT_PORGI 275 11 -

Motif 4 width=25
Element Seqn Id St Int Rpt
KELSQNQPVYYQGVGKVGGHAFVID Q54962 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID SPEB_STRPY 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q57212 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q57211 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q57202 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q57082 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q57024 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q54968 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q54967 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q54964 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q54963 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q54965 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q54966 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q54960 321 13 -
KELSQNQPVYYQGVGKVGGHAFVID Q54961 321 13 -
KELSQNQPVYYEGVGKVGGHAFVID STCP_STRPY 176 13 -
KELAENRPVYYAGADGSMGHAFVCD PRTT_PORGI 308 13 -

Motif 5 width=22
Element Seqn Id St Int Rpt
YHVNWGWGGVSDGFFRLDALNP Q54962 353 7 -
YHVNWGWGGVSDGFFRLDALNP SPEB_STRPY 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q57212 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q57211 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q57202 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q57082 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q57024 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q54968 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q54967 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q54964 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q54963 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q54965 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q54966 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q54960 353 7 -
YHVNWGWGGVSDGFFRLDALNP Q54961 353 7 -
YHVDWGWGGVSDGFFRLDALNP STCP_STRPY 208 7 -
FHFNWGWGGMSNGNFYLNLLNP PRTT_PORGI 340 7 -

Motif 6 width=22
Element Seqn Id St Int Rpt
SALGTGGGAGGFNGYQSAVVGI Q54962 375 0 -
SALGTGGGAGGFNGYQSAVVGI SPEB_STRPY 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q57212 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q57211 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q57202 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q57082 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q57024 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q54968 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q54967 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q54964 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q54963 375 0 -
SALGTGGGAGGFNGYQSAVIGI Q54965 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q54966 375 0 -
SALGTGGGADGFNGYQSAVVGI Q54960 375 0 -
SALGTGGGAGGFNGYQSAVVGI Q54961 375 0 -
SALGTGGGAGGFNGYESAVVGI STCP_STRPY 230 0 -
GSLGTGAGDGGYSTDQEVVIGI PRTT_PORGI 362 0 -