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PR00781

Identifier
LIPOSIGPTASE  [View Relations]  [View Alignment]  
Accession
PR00781
No. of Motifs
3
Creation Date
10-JUN-1997  (UPDATE 27-JUN-1999)
Title
Lipoprotein signal peptidase family (A8) signature
Database References

PROSITE; PS00855 SPASE_II
BLOCKS; BL00855
INTERPRO; IPR001872
Literature References
1. RAWLINGS, N.D. AND BARRETT, A.J.
Families of aspartic peptidases, and those of unknown catalytic mechanism.
METHODS ENZYMOL. 248 105-120 (1995).
 
2. TOKUNAGA, M., LORANGER, J.M. AND WU, H.C.
Prolipoprotein modification and processing enzymes in Escherichia coli.
J.BIOL.CHEM. 259 3825-3830 (1984).

Documentation
Bacterial cell walls contain large amounts of murein lipoprotein, a small 
protein that is both N-terminally bound to lipid and attached to membrane 
peptidoglycan (murein) through the epsilon-amino group of its C-terminal
lysine residue [1]. Secretion of this lipoprotein is facilitated by the
action of lipoprotein signal peptidase (also known as leader peptidase II),
located in the inner membrane [1,2]. The enzyme is inhibited by globomycin
and also by pepstatin, suggesting that it is an aspartic peptidase [1].
The catalytic residues have not been identified, but three conserved
aspartates can be identified from sequence alignments.
 
LIPOSIGPTASE is a 3-element fingerprint that provides a signature for the
lipoprotein signal peptidase family (A8) of aspartate endopeptidases. The 
fingerprint was derived from an initial alignment of 7 sequences: the
motifs were drawn from conserved regions spanning virtually the full
alignment length - motif 2 includes the region encoded by PROSITE pattern
SPASE_II (PS00855), which contains one of the conserved aspartate residues;
and motif 3 contains a second conserved Asp. Two iterations on OWL29.3 were
required to reach convergence, at which point a true set comprising 11
sequences was identified. A single partial match was also found, LLU63724,
a fragment that lacks the region of sequence bearing motif 3.
 
An update on SPTR37_9f identified a true set of 18 sequences, and 1
partial match.
Summary Information
  18 codes involving  3 elements
1 codes involving 2 elements
Composite Feature Index
3181818
2011
123
True Positives
LSPA_BACSU    LSPA_ECOLI    LSPA_ENTAE    LSPA_HAEIN    
LSPA_HELPY LSPA_MYCGE LSPA_MYCPN LSPA_MYCTU
LSPA_PSEFL LSPA_STAAU LSPA_STACA LSPA_SYNY3
O51425 O52213 O67692 O83943
O84413 ZNUB_ECOLI
True Positive Partials
Codes involving 2 elements
O87781
Sequence Titles
LSPA_BACSU  LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - BACILLUS SUBTILIS. 
LSPA_ECOLI LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - ESCHERICHIA COLI.
LSPA_ENTAE LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - ENTEROBACTER AEROGENES (AEROBACTER AEROGENES).
LSPA_HAEIN LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - HAEMOPHILUS INFLUENZAE.
LSPA_HELPY LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
LSPA_MYCGE PUTATIVE LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - MYCOPLASMA GENITALIUM.
LSPA_MYCPN PUTATIVE LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - MYCOPLASMA PNEUMONIAE.
LSPA_MYCTU PROBABLE LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - MYCOBACTERIUM TUBERCULOSIS.
LSPA_PSEFL LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - PSEUDOMONAS FLUORESCENS.
LSPA_STAAU LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - STAPHYLOCOCCUS AUREUS.
LSPA_STACA LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - STAPHYLOCOCCUS CARNOSUS.
LSPA_SYNY3 LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
O51425 SIGNAL PEPTIDASE II (LSP) - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).
O52213 LSPA - SERRATIA MARCESCENS.
O67692 LIPOPROTEIN SIGNAL PEPTIDASE (EC 3.4.23.36) (PROLIPOPROTEIN SIGNAL PEPTIDASE) (SIGNAL PEPTIDASE II) (SPASE II) - AQUIFEX AEOLICUS.
O83943 SIGNAL PEPTIDASE II (LSP) - TREPONEMA PALLIDUM.
O84413 LIPOPROTEIN SIGNAL PEPTIDASE - CHLAMYDIA TRACHOMATIS.
ZNUB_ECOLI HIGH-AFFINITY ZINC UPTAKE SYSTEM MEMBRANE PROTEIN ZNUB - ESCHERICHIA COLI.

O87781 SPASE II - MYXOCOCCUS XANTHUS.
Scan History
OWL29_3    2  110  NSINGLE    
SPTR37_9f 3 100 NSINGLE
Initial Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
NKGVGFSLL B64223 72 72 -
NKGVGFSLL S73868 76 76 -
NTGAAWGIL BSU488702 45 45 -
NYGAAFSFL LSPA_ECOLI 53 53 -
NTGAAFSFL LSPA_PSEFL 55 55 -
NNGAAWGIL LSPA_STAAU 52 52 -
NSGAAFSMA LSPA_MYCTU 78 78 -

Motif 2 width=16
Element Seqn Id St Int Rpt
LALVLGGALGNLYDRI LSPA_PSEFL 103 39 -
ISLLFAGALGNFIDRI LSPA_STAAU 96 35 -
LGMILGGAMGNLVDRF LSPA_MYCTU 120 33 -
ITTLAFGSLGNFFDRL B64223 114 33 -
ITTLAFGSLGNFFDRL S73868 118 33 -
LGLMLGGAIGNFIDRA BSU488702 89 35 -
YALIIGGALGNLFDRL LSPA_ECOLI 101 39 -

Motif 3 width=17
Element Seqn Id St Int Rpt
FNFADCCITFGFIGLFF B64223 149 19 -
FNIADSSLCVGVMLLFI BSU488702 125 20 -
FNLADTAICVGAALIVL LSPA_ECOLI 137 20 -
FNFADSAITVGAIMLAL LSPA_PSEFL 140 21 -
FNIADSSLTIGVILIII LSPA_STAAU 132 20 -
FNVADPSVVGGAILLVI LSPA_MYCTU 158 22 -
FNLADCCITFSFIGLFL S73868 153 19 -
Final Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
NYGAAFSFL LSPA_ENTAE 53 53 -
NYGAAFSFL LSPA_ECOLI 53 53 -
NTGAAFSFL LSPA_PSEFL 55 55 -
NKGVAFGLL O67692 51 51 -
NYGAAFSFL LSPA_HAEIN 50 50 -
NTGAAFSLF O52213 54 54 -
NNGAAWGIL LSPA_STAAU 52 52 -
NTGAAWGIL LSPA_BACSU 45 45 -
NSGAAFSMA LSPA_MYCTU 78 78 -
NTGAAFSAF LSPA_SYNY3 56 56 -
NNGAAWGIL LSPA_STACA 52 52 -
NEGAAFGLF O84413 58 58 -
NVGAAFSIG O83943 67 67 -
NKGVGFSLL LSPA_MYCGE 72 72 -
NKGVGFSLL LSPA_MYCPN 76 76 -
NKGVAFSLL LSPA_HELPY 48 48 -
NTGILFSMG O51425 54 54 -
LLGVAFGLL ZNUB_ECOLI 43 43 -

Motif 2 width=16
Element Seqn Id St Int Rpt
YALIIGGALGNLFDRL LSPA_ENTAE 101 39 -
YALIIGGALGNLFDRL LSPA_ECOLI 101 39 -
LALVLGGALGNLYDRI LSPA_PSEFL 103 39 -
MGLIGGGALGNLYDRL O67692 98 38 -
YALIIGGALANMVDRA LSPA_HAEIN 98 39 -
YSLILGGAMGNLIDRV O52213 100 37 -
ISLLFAGALGNFIDRI LSPA_STAAU 96 35 -
LGLMLGGAIGNFIDRA LSPA_BACSU 89 35 -
LGMILGGAMGNLVDRF LSPA_MYCTU 120 33 -
YGCILAGAVGNGIDRF LSPA_SYNY3 99 34 -
ISLLFAGALGNFIDRM LSPA_STACA 96 35 -
LTLILAGALGNVGDIL O84413 103 36 -
VSGIIGGGIGNLIDRF O83943 116 40 -
ITTLAFGSLGNFFDRL LSPA_MYCGE 114 33 -
ITTLAFGSLGNFFDRL LSPA_MYCPN 118 33 -
FGMVFGAGVSNVLDRF LSPA_HELPY 92 35 -
LLLIFSGGVGNVIDRL O51425 101 38 -
VTLLLAGGLVWLEKRP ZNUB_ECOLI 63 11 -

Motif 3 width=17
Element Seqn Id St Int Rpt
FNLADSAICIGAALIVL LSPA_ENTAE 137 20 -
FNLADTAICVGAALIVL LSPA_ECOLI 137 20 -
FNFADSAITVGAIMLAL LSPA_PSEFL 140 21 -
FNIADASISIGIALLIL O67692 134 20 -
FNIADIAICIGAGLLVL LSPA_HAEIN 134 20 -
FNLADIAIVLGALLFVS O52213 136 20 -
FNIADSSLTIGVILIII LSPA_STAAU 132 20 -
FNIADSSLCVGVMLLFI LSPA_BACSU 125 20 -
FNVADPSVVGGAILLVI LSPA_MYCTU 158 22 -
FNLADVSINIGIAALLW LSPA_SYNY3 133 18 -
FNGADSSLTIGVILVLI LSPA_STACA 132 20 -
FNLADAFISIGTLLLIG O84413 139 20 -
FNIADAVIMTCGLLLII O83943 157 25 -
FNFADCCITFGFIGLFF LSPA_MYCGE 149 19 -
FNLADCCITFSFIGLFL LSPA_MYCPN 153 19 -
FNFADVMIDVGVGVLLL LSPA_HELPY 127 19 -
FNFADSYVVIGMILFLV O51425 142 25 -
DTPAGPSVVLCAALLFI ZNUB_ECOLI 236 157 -