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PR00757

Identifier
AMINEOXDASEF  [View Relations]  [View Alignment]  
Accession
PR00757
No. of Motifs
9
Creation Date
01-SEP-1997  (UPDATE 12-JUN-1999)
Title
Flavin-containing amine oxidase signature
Database References

INTERPRO; IPR001613
UMBBD; e0339
Literature References
1. ZHU, Q.S., GRIMSBY, J., CHEN, K. AND SHIH, J.C.
Promoter organization and activity of human monoamine oxidase (MAO)
A and B genes.
J.NEUROSCIENCE 12(11) 4437-4446 (1992).
 
2. HSU, Y.P., WEYLER, W., CHEN, S., SIMS, K.B., RINEHART, W.B., UTTERBACK,
M.C., POWELL, J.F. AND BREAKEFIELD, X.O.
Structural features of human monoamine oxidase A elucidated from cDNA and
peptide sequences. 
J.NEUROCHEM. 51(4) 1321-1324 (1988).
 
3. BACH, A.W., LAN, N.C., JOHNSON, D.L., ABELL, C.W., BEMBENEK, M.E., KWAN,
S.W., SEEBURG, P.H. AND SHIH, J.C.
cDNA cloning of human liver monoamine oxidase A and B: molecular basis of 
differences in enzymatic properties. 
PROC.NATL.ACAD.SCI.U.S.A. 85(13) 4934-4938 (1988).

Documentation
Monoamine oxidases (MAO) A and B are encoded by two genes derived from
a common ancestral gene [1]. The enzymes catalyse the oxidative deamination
of biogenic and xenobiotic amines and have important roles in the metabolism
of neuroactive and vasoactive amines in the central nervous system and
peripheral tissues [2]. MAO-A preferentially oxidises biogenic amines such
as 5-hydroxytryptamine, norepinephrine and epinephrine. MAO-A deficiency
has been linked to Brunner's syndrome, a form of X-linked nondysmorphic
mild mental retardation [2].
 
The protein contains two similarly-sized subunits, one of which contains
covalently-bound flavin adenine dinucleotide (FAD). The FAD binding site
lies near the C-terminus; at the N-terminus are features characteristic
of the ADP-binding fold, suggesting that this region is also involved in
FAD binding [2]. The A and B forms of the enzyme share 70% sequence identity;
both contain the pentapeptide Ser-Gly-Gly-Cys-Tyr, the cysteine of which
binds FAD [3].
 
AMINEOXDASEF is a 9-element fingerprint that provides a signature for
flavin-containing amine oxidases. The fingerprint was derived from an
initial alignment of 7 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length - motif 1 lies in the
N-terminal putative nucleotide-binding region; and motif 6 contains the
conserved C-terminal pentapeptide, the cysteine of which binds the FAD
cofactor. A single iteration on OWL29.4 was required to reach convergence,
no further sequences being identified beyond the starting set. Several
partial matches were also found, all of which are fragments and related
oxidases that fail to match one or more motifs.
 
An update on SPTR37_9f identified a true set of 6 sequences, and 7
partial matches.
Summary Information
   6 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
1 codes involving 4 elements
0 codes involving 3 elements
4 codes involving 2 elements
Composite Feature Index
9666666666
8000000000
7000000000
6000000000
5101010110
4000011110
3000000000
2300010220
123456789
True Positives
AOFA_BOVIN    AOFA_HUMAN    AOFA_RAT      AOFB_HUMAN    
AOFB_RAT AOF_ONCMY
True Positive Partials
Codes involving 5 elements
AOFH_MYCTU
Codes involving 4 elements
Y782_SYNY3
Codes involving 2 elements
AOFN_ASPNG FIG1_MOUSE O64411 PUO_MICRU
Sequence Titles
AOFA_BOVIN  AMINE OXIDASE [FLAVIN-CONTAINING] A (EC 1.4.3.4) (MONOAMINE OXIDASE) (MAO-A) - BOS TAURUS (BOVINE). 
AOFA_HUMAN AMINE OXIDASE [FLAVIN-CONTAINING] A (EC 1.4.3.4) (MONOAMINE OXIDASE) (MAO-A) - HOMO SAPIENS (HUMAN).
AOFA_RAT AMINE OXIDASE [FLAVIN-CONTAINING] A (EC 1.4.3.4) (MONOAMINE OXIDASE) (MAO-A) - RATTUS NORVEGICUS (RAT).
AOFB_HUMAN AMINE OXIDASE [FLAVIN-CONTAINING] B (EC 1.4.3.4) (MONOAMINE OXIDASE) (MAO-B) - HOMO SAPIENS (HUMAN).
AOFB_RAT AMINE OXIDASE [FLAVIN-CONTAINING] B (EC 1.4.3.4) (MONOAMINE OXIDASE) (MAO-B) - RATTUS NORVEGICUS (RAT).
AOF_ONCMY AMINE OXIDASE [FLAVIN-CONTAINING] (EC 1.4.3.4) (MONOAMINE OXIDASE) (MAO) - ONCORHYNCHUS MYKISS (RAINBOW TROUT) (SALMO GAIRDNERI).

AOFH_MYCTU PUTATIVE FLAVIN-CONTAINING MONOAMINE OXIDASE MTV014.14 (EC 1.4.3.-) - MYCOBACTERIUM TUBERCULOSIS.

Y782_SYNY3 HYPOTHETICAL 51.4 KD PROTEIN SLR0782 - SYNECHOCYSTIS SP. (STRAIN PCC 6803).

AOFN_ASPNG MONOAMINE OXIDASE N (EC 1.4.3.4) (MAO-N) - ASPERGILLUS NIGER.
FIG1_MOUSE FIG-1 PROTEIN PRECURSOR - MUS MUSCULUS (MOUSE).
O64411 POLYAMINE OXIDASE - ZEA MAYS (MAIZE).
PUO_MICRU PUTRESCINE OXIDASE (EC 1.4.3.10) - MICROCOCCUS RUBENS.
Scan History
OWL29_4    1  100  NSINGLE    
SPTR37_9f 2 100 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
DVGLIGGGISGLAAAKLLSE AOFA_RAT 15 15 -
DVVVIGGGISGLSAAKLLTE AOFA_HUMAN 15 15 -
DVIVVGGGISGMAAAKLLHD AOFB_RAT 6 6 -
DVIVIGGGISGLSAAKLLKE AOF_ONCMY 8 8 -
DVVVIGGGISGLAAAKLLSE S45812 9 9 -
DVVVVGGGISGMAAAKLLHD AOFB_HUMAN 6 6 -
DVVVIGGGISGLSAAKLLAE AOFA_BOVIN 15 15 -

Motif 2 width=23
Element Seqn Id St Int Rpt
VKGKSYPFKGSFPPMWNPFALMD AOF_ONCMY 94 66 -
VKGKTYPFRGAFPPVWNPLAYLD S45812 95 66 -
VKGKTYPFRGAFPPVWNPIAYLD AOFA_HUMAN 101 66 -
VKGKTYPFRGAFPPVWNPLAYLD AOFA_RAT 101 66 -
VKGKTYPFRGAFPPVWNPIAYLD AOFA_BOVIN 101 66 -
VKGKSYPFRGPFPPVWNPITYLD AOFB_HUMAN 92 66 -
VKGKSYAFRGPFPPVWNPITYLD AOFB_RAT 92 66 -

Motif 3 width=22
Element Seqn Id St Int Rpt
TSEPHEVSALWFLWYVRQCGGT AOFA_RAT 183 59 -
TSEPHEVSALWFLWYVKQCGGT AOFA_HUMAN 183 59 -
TSEPHEVSALWFLWYVKQCGGT AOFA_BOVIN 183 59 -
TAETHEVSALWFLWYVKQCGGT AOFB_HUMAN 174 59 -
TAETHEVSALWFLWYVKQCGGT AOFB_RAT 174 59 -
TSEPHEVSALWFLWYVKQCGGT AOF_ONCMY 176 59 -
TSEPHEVSALWFLWYVRQCGGT S45812 177 59 -

Motif 4 width=23
Element Seqn Id St Int Rpt
RIISTTNGGQERKFIGGSGQVSE AOFB_RAT 197 1 -
RIFSVTNGGQERKFVGGSGQVSE S45812 200 1 -
RIFSVTNGGQERKFVGGSGQVSE AOFA_HUMAN 206 1 -
RIFSVTNGGQERKFVGGSGQVSE AOFA_RAT 206 1 -
RIFSITNGGQERKFVGGSGQVSE AOFA_BOVIN 206 1 -
RIISTTNGGQERKFVGGSGQVSE AOFB_HUMAN 197 1 -
RIFSTTNGGQERKFLGGSSQISE AOF_ONCMY 199 1 -

Motif 5 width=20
Element Seqn Id St Int Rpt
VIKCMMYYKEAFWKKKDYCG AOFA_BOVIN 303 74 -
VIKCIVYYKEPFWRKKDYCG AOFB_HUMAN 294 74 -
VIKCMVYYKEPFWRKKDFCG AOFB_RAT 294 74 -
VIKCIVYYRENFWRKKGYCG AOF_ONCMY 296 74 -
VIKCMMYYKEAFWKKKDYCG AOFA_HUMAN 303 74 -
VIKCMVYYKEAFWKKKDYCG S45812 297 74 -
VIKCMVYYKEAFWKKKDYCG AOFA_RAT 303 74 -

Motif 6 width=20
Element Seqn Id St Int Rpt
PVHYEEKNWCQEQYSGGCYT AOFA_BOVIN 389 66 -
PVHYEEKNWCEEQYSGGCYT AOFB_RAT 380 66 -
PVHYEEKNWCEEEYSGGCYT AOF_ONCMY 382 66 -
PVHYEEKNWCEEQYSGGCYT S45812 383 66 -
PVHYEEKNWCEEQYSGGCYT AOFA_HUMAN 389 66 -
PVHYEEKNWCEEQYSGGCYT AOFA_RAT 389 66 -
PVHYEEKNWCEEQYSGGCYT AOFB_HUMAN 380 66 -

Motif 7 width=23
Element Seqn Id St Int Rpt
YFPPGILTQYGRVLRQPVDRIYF AOFB_HUMAN 401 1 -
YFPPGILTQYGKVLREPVGRLYF AOF_ONCMY 403 1 -
YFPPGIMTQYGRVIRQPVGRIYF S45812 404 1 -
YFPPGIMTQYGRVIRQPVGRIFF AOFA_HUMAN 410 1 -
YFPPGIMTQYGRVIRQPVGRIYF AOFA_RAT 410 1 -
YFPPGIMTQYGRVIRQPVGRIYF AOFA_BOVIN 410 1 -
YFPPGILTQYGRVLRQPVGKIFF AOFB_RAT 401 1 -

Motif 8 width=18
Element Seqn Id St Int Rpt
WSGYMEGAVEAGERAARE AOFB_RAT 432 8 -
WSGYMEGAVEAGERAARE S45812 435 8 -
WSGYMEGAVEAGERAARE AOFA_HUMAN 441 8 -
WSGYMEGAVEAGERAARE AOFA_RAT 441 8 -
WSGYMEGAVEAGERAARE AOFA_BOVIN 441 8 -
WSGYMEGAVEAGERAARE AOFB_HUMAN 432 8 -
WSGYMEGAVQAGERAARE AOF_ONCMY 434 8 -

Motif 9 width=23
Element Seqn Id St Int Rpt
IWVEEPESKDVPAIEITHTFLER AOFA_RAT 471 12 -
IWQSEPESVDVPAQPITTTFLER AOFB_HUMAN 462 12 -
IWQPEPESVDVPARPITNTFLER AOFB_RAT 462 12 -
IWQTEPESVEVPALPFVTTFWER AOF_ONCMY 464 12 -
IWVQEPESKDVPAVEITHTFWER AOFA_HUMAN 471 12 -
IWVEEPESKDVPAIEITHTFLER S45812 465 12 -
IWIQEPEAEDVPAVEITPSFWER AOFA_BOVIN 471 12 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
DVVVIGGGISGLSAAKLLTE AOFA_HUMAN 15 15 -
DVGLIGGGISGLAAAKLLSE AOFA_RAT 15 15 -
DVVVIGGGISGLSAAKLLAE AOFA_BOVIN 15 15 -
DVVVVGGGISGMAAAKLLHD AOFB_HUMAN 6 6 -
DVIVVGGGISGMAAAKLLHD AOFB_RAT 6 6 -
DVIVIGGGISGLSAAKLLKE AOF_ONCMY 8 8 -

Motif 2 width=23
Element Seqn Id St Int Rpt
VKGKTYPFRGAFPPVWNPIAYLD AOFA_HUMAN 101 66 -
VKGKTYPFRGAFPPVWNPLAYLD AOFA_RAT 101 66 -
VKGKTYPFRGAFPPVWNPIAYLD AOFA_BOVIN 101 66 -
VKGKSYPFRGPFPPVWNPITYLD AOFB_HUMAN 92 66 -
VKGKSYAFRGPFPPVWNPITYLD AOFB_RAT 92 66 -
VKGKSYPFKGSFPPMWNPFALMD AOF_ONCMY 94 66 -

Motif 3 width=22
Element Seqn Id St Int Rpt
TSEPHEVSALWFLWYVKQCGGT AOFA_HUMAN 183 59 -
TSEPHEVSALWFLWYVRQCGGT AOFA_RAT 183 59 -
TSEPHEVSALWFLWYVKQCGGT AOFA_BOVIN 183 59 -
TAETHEVSALWFLWYVKQCGGT AOFB_HUMAN 174 59 -
TAETHEVSALWFLWYVKQCGGT AOFB_RAT 174 59 -
TSEPHEVSALWFLWYVKQCGGT AOF_ONCMY 176 59 -

Motif 4 width=23
Element Seqn Id St Int Rpt
RIFSVTNGGQERKFVGGSGQVSE AOFA_HUMAN 206 1 -
RIFSVTNGGQERKFVGGSGQVSE AOFA_RAT 206 1 -
RIFSITNGGQERKFVGGSGQVSE AOFA_BOVIN 206 1 -
RIISTTNGGQERKFVGGSGQVSE AOFB_HUMAN 197 1 -
RIISTTNGGQERKFIGGSGQVSE AOFB_RAT 197 1 -
RIFSTTNGGQERKFLGGSSQISE AOF_ONCMY 199 1 -

Motif 5 width=20
Element Seqn Id St Int Rpt
VIKCMMYYKEAFWKKKDYCG AOFA_HUMAN 303 74 -
VIKCMVYYKEAFWKKKDYCG AOFA_RAT 303 74 -
VIKCMMYYKEAFWKKKDYCG AOFA_BOVIN 303 74 -
VIKCIVYYKEPFWRKKDYCG AOFB_HUMAN 294 74 -
VIKCMVYYKEPFWRKKDFCG AOFB_RAT 294 74 -
VIKCIVYYRENFWRKKGYCG AOF_ONCMY 296 74 -

Motif 6 width=20
Element Seqn Id St Int Rpt
PVHYEEKNWCEEQYSGGCYT AOFA_HUMAN 389 66 -
PVHYEEKNWCEEQYSGGCYT AOFA_RAT 389 66 -
PVHYEEKNWCQEQYSGGCYT AOFA_BOVIN 389 66 -
PVHYEEKNWCEEQYSGGCYT AOFB_HUMAN 380 66 -
PVHYEEKNWCEEQYSGGCYT AOFB_RAT 380 66 -
PVHYEEKNWCEEEYSGGCYT AOF_ONCMY 382 66 -

Motif 7 width=23
Element Seqn Id St Int Rpt
YFPPGIMTQYGRVIRQPVGRIFF AOFA_HUMAN 410 1 -
YFPPGIMTQYGRVIRQPVGRIYF AOFA_RAT 410 1 -
YFPPGIMTQYGRVIRQPVGRIYF AOFA_BOVIN 410 1 -
YFPPGILTQYGRVLRQPVDRIYF AOFB_HUMAN 401 1 -
YFPPGILTQYGRVLRQPVGKIFF AOFB_RAT 401 1 -
YFPPGILTQYGKVLREPVGRLYF AOF_ONCMY 403 1 -

Motif 8 width=18
Element Seqn Id St Int Rpt
WSGYMEGAVEAGERAARE AOFA_HUMAN 441 8 -
WSGYMEGAVEAGERAARE AOFA_RAT 441 8 -
WSGYMEGAVEAGERAARE AOFA_BOVIN 441 8 -
WSGYMEGAVEAGERAARE AOFB_HUMAN 432 8 -
WSGYMEGAVEAGERAARE AOFB_RAT 432 8 -
WSGYMEGAVQAGERAARE AOF_ONCMY 434 8 -

Motif 9 width=23
Element Seqn Id St Int Rpt
IWVQEPESKDVPAVEITHTFWER AOFA_HUMAN 471 12 -
IWVEEPESKDVPAIEITHTFLER AOFA_RAT 471 12 -
IWIQEPEAEDVPAVEITPSFWER AOFA_BOVIN 471 12 -
IWQSEPESVDVPAQPITTTFLER AOFB_HUMAN 462 12 -
IWQPEPESVDVPARPITNTFLER AOFB_RAT 462 12 -
IWQTEPESVEVPALPFVTTFWER AOF_ONCMY 464 12 -