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PR00754

Identifier
AEROLYSIN  [View Relations]  [View Alignment]  
Accession
PR00754
No. of Motifs
9
Creation Date
25-AUG-1997  (UPDATE 06-JUN-1999)
Title
Aerolysin signature
Database References

PROSITE; PS00274 AEROLYSIN
BLOCKS; BL00274
PFAM; PF01117 Aerolysin
INTERPRO; IPR001776
Literature References
1. PARKER, M.W., BUCKLEY, J.T., POSTMA, J.P., TUCKER, A.D., LEONARD, K.,
PATTUS, F. AND TSERNOGLOU, D.
Structure of the aeromonas toxin proaerolysin in its water-soluble and
membrane-channel states. 
NATURE 367 292-295 (1994).

Documentation
Aerolysin is responsible for the pathogenicity of Aeromonas hydrophila, a
bacterium associated with diarrhoeal diseases and deep wound infections [1].
In common with other microbial toxins, the protein changes in a multi-step
process from a water-soluble form to produce a transmembrane channel that
destroys sensitive cells by breaking their permeability barriers [1].
 
The structure of proaerolysin has been determined to 2.8A resolution and
shows the protoxin to adopt a novel fold [1]. Images of an aerolysin 
oligomer derived from electron microscopy have helped to construct a
model of the protein and to outline a mechanism by which it might insert
into lipid bilayers to form ion channels [1].
 
AEROLYSIN is a 9-element fingerprint that provides a signature for the
aerolysins. The fingerprint was derived from an initial alignment of 10
sequences: the motifs were drawn from conserved regions spanning virtually
the full alignment length. A single iteration on OWL29.4 was required to
reach convergence, no further sequences being identified beyond the
starting set. A single partial match was found, CLOALPTOX, a related
alpha-toxin from Clostridium septicum that matches motifs 4 and 6.
 
An update on SPTR37_9f identified a true set of 10 sequences.
Summary Information
10 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9101010101010101010
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
AER3_AERHY    AER4_AERHY    AER5_AERHY    AERA_AERHY    
AERA_AERSA AERA_AERSO AERA_AERTR O85370
P94128 Q44063
Sequence Titles
AER3_AERHY  AEROLYSIN 3 PRECURSOR (HEMOLYSIN 3) - AEROMONAS HYDROPHILA. 
AER4_AERHY AEROLYSIN 4 PRECURSOR (HEMOLYSIN 4) - AEROMONAS HYDROPHILA.
AER5_AERHY AEROLYSIN 5 PRECURSOR (HEMOLYSIN 5) - AEROMONAS HYDROPHILA.
AERA_AERHY AEROLYSIN PRECURSOR - AEROMONAS HYDROPHILA.
AERA_AERSA AEROLYSIN PRECURSOR (HEMOLYSIN 3) - AEROMONAS SALMONICIDA.
AERA_AERSO AEROLYSIN PRECURSOR (HEMOLYSIN) - AEROMONAS SOBRIA.
AERA_AERTR AEROLYSIN PRECURSOR - AEROMONAS TROTA.
O85370 PREPROAEROLYSIN - AEROMONAS TROTA.
P94128 HEMOLYSIN - AEROMONAS CAVIAE.
Q44063 AEROLYSIN PRECURSOR - AEROMONAS HYDROPHILA.
Scan History
OWL29_4    1  100  NSINGLE    
SPTR37_9f 2 11 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
YRPLTRDEAMSIKGNLVSR S26576 47 47 -
YRPLTRDEAMSIKGNLVSR I39672 48 48 -
YRPVNREEAQSVKSNIVGM S26573 46 46 -
YRPINREEAQSVRNNIVGM ACU40711 46 46 -
YRPITREEAQSVKSNIVNM S26575 46 46 -
YRPITREEAQSVKSNIVNM I39541 46 46 -
YRPVNREEAQSVKSNIVGM AERA_AERHY 46 46 -
YRPINREEAQSVRNNIVAM AERA_AERSO 46 46 -
YRPVNREEAQSIKSNIVGM I39591 46 46 -
YRPVNREEAQSVKSNIVGM AEOHFPA 46 46 -

Motif 2 width=20
Element Seqn Id St Int Rpt
MGQWQITGLADRWVIMGPGY S26576 66 0 -
MGQWQISGLANGWVIMGPGY AEOHFPA 65 0 -
MGQWQISGLANGWVIMGPGY I39591 65 0 -
MGQWQISGLANGWVIMGPGY I39541 65 0 -
MGQWQISGLANGWVIMGPVY S26575 65 0 -
MGQWQISGLANNWVIMGSGY ACU40711 65 0 -
MGQWQITGLADRWVIMGPGY I39672 67 0 -
MGQWQISGLANGWVIMGPGY S26573 65 0 -
MGQWQISGLANNWVILGPGY AERA_AERSO 65 0 -
MGQWQISGLANGWVIMGPGY AERA_AERHY 65 0 -

Motif 3 width=21
Element Seqn Id St Int Rpt
NGEIKPGSASNTWCYPVNPVT S26575 85 0 -
NGEIKPGSASDTWCYPTKPVT ACU40711 85 0 -
NGEIKQGTAGETWCYPNSPVS I39672 87 0 -
NGEIKQGTAGETWCYPNSPVS S26576 86 0 -
NGEIKPGKASTTWCYPTRPAT AERA_AERSO 85 0 -
NGEIKPGTASNTWCYPTNPVT AERA_AERHY 85 0 -
NGEIKPGTASNTWCYPTNPVT AEOHFPA 85 0 -
NGEIKPGSASSTWCYPTNPAT S26573 85 0 -
NGEIKPGSASSTWCYPTNPAT I39591 85 0 -
NGEIKPGSASNTWCYPVNPVT I39541 85 0 -

Motif 4 width=23
Element Seqn Id St Int Rpt
SYLAHYLGYAWVGGNHSPYVGED S26576 141 34 -
SYLAHYLGYAWVGGNHSQYVGED AERA_AERHY 140 34 -
SYLAHYLGYAWVGGNHSQYVGED S26573 140 34 -
SYLAHYLGYAWVGGNHSQYVGED AEOHFPA 140 34 -
SYLAHYLGYAWVGGNHSQYVGED I39591 140 34 -
SYLAHYLGYAWVGGNHSQYVGED I39541 140 34 -
SYLAHYLGYAWVGGNHSQYVGED S26575 140 34 -
SYLAHYLGYAWVGGNHSPYVGED ACU40711 140 34 -
SYLAHYLGYAWVGGNHSPYVGED I39672 142 34 -
SYLAHYLGYAWVGGDHSQFVGDD AERA_AERSO 140 34 -

Motif 5 width=25
Element Seqn Id St Int Rpt
KNKFQWPLVGETELAIEIAASQSWA S26576 265 101 -
KNKFQWPLVGETELAIEIAASQSWA I39672 266 101 -
KNKFKWPLVGETELSIEIAASQSWA ACU40711 265 102 -
KNKFKWPLVGETELSIEIAANQSWA S26575 265 102 -
KNKFKWPLVGETELSIEIAANQSWA I39541 265 102 -
KNKFKWPLVGETELSIEIAANQSWA I39591 265 102 -
KNKFKWPLVGETQLSIEIRANQSWA AEOHFPA 265 102 -
KNKFKWPLVGETELSIEIAANQSWA S26573 265 102 -
KNKFKWPLVGETEVSIEIAANQSWA AERA_AERSO 265 102 -
KNKFKWPLVGETELSIEIAANQSWA AERA_AERHY 265 102 -

Motif 6 width=24
Element Seqn Id St Int Rpt
YDLTLNGFLRWGGNAWYTHPDNRP ACU40711 337 47 -
YDLTLSGFLRWGGNAWYTHPDNRP S26573 337 47 -
YDLTLSGFLRWGGNAWYTHPDNRP AEOHFPA 337 47 -
YDLTLSGFLRWGGNAWYTHPDNRP I39591 337 47 -
YDLTLSGFLRWGGNAWYTHPDNRP I39541 337 47 -
YDLTLSGFLRWGGNAWYTHPDNRP S26575 337 47 -
YDLTMKGFLRWGGNAWYTHPDNRP I39672 338 47 -
YDLTMKGFLRWGGNAWYTHPDNRP S26576 337 47 -
YDLTLSGFLRWGGNAWYTHPDNRP AERA_AERHY 337 47 -
YDLTFNGFLRWGGNAWHTHPEDRP AERA_AERSO 337 47 -

Motif 7 width=20
Element Seqn Id St Int Rpt
WDKRYIPGEVKWWDWNWTIQ S26575 382 21 -
WDKRYIPGEVKWWDWNWTIQ ACU40711 382 21 -
WDKRYLPGEMKWWDWNWAIQ AERA_AERSO 382 21 -
WDKRYIPGEVKWWDWNWTIQ AERA_AERHY 382 21 -
VDKRYIPGEVKWWDWNWTIS S26576 382 21 -
VDKRYIPGEVKWWDWNWTIS I39672 383 21 -
WDKRYIPGEVKWWDWNWTIQ S26573 382 21 -
WDKRYIPGEVKWWDWNWTIQ AEOHFPA 382 21 -
WDKRYIPGEVKWWDWNWTIQ I39591 382 21 -
WDKRYIPGEVKWWDWNWTIQ I39541 382 21 -

Motif 8 width=19
Element Seqn Id St Int Rpt
GLSTMQNNLARVLRPVRAG I39541 404 2 -
GADTMKNALARVLRPVRAS ACU40711 404 2 -
GLSTMQNNLGRVLRPIRSA I39672 405 2 -
GLSTMQNNLGRVLRPIRSA S26576 404 2 -
GLSTMQNNLARVLRPVRAG AEOHFPA 404 2 -
GLPTMQNNLAKVLRPVRAG S26573 404 2 -
GLSTMQNNLARVLRPVRAG AERA_AERHY 404 2 -
GLATMQDSLARVLRPVRAS AERA_AERSO 404 2 -
GLPTMQNNLAKVLRPVRAG I39591 404 2 -
GLSTMQNNLARVLRPVRAG S26575 404 2 -

Motif 9 width=20
Element Seqn Id St Int Rpt
ITGDFSAESQFAGNIEIGAP I39541 423 0 -
ITGDFSAESQFAGNIEIGAP I39591 423 0 -
ITGDFSAESQFAGNIEIGAP AERA_AERHY 423 0 -
ITGDFRAESQFAGNIEIGTP AERA_AERSO 423 0 -
ITGDFSAESQFAGNIEIGAP AEOHFPA 423 0 -
ITGDFSAESQFAGNIEIGAP S26573 423 0 -
VTGDFYAESQFAGDIEIGQP S26576 423 0 -
VTGDFYAESQFAGDIEIGQP I39672 424 0 -
ITGDFHAESQFAGNIEIGAA ACU40711 423 0 -
ITGDFSAESQFAGNIEIGAP S26575 423 0 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
YRPVNREEAQSVKSNIVGM AERA_AERHY 46 46 -
YRPITREEAQSVKSNIVNM Q44063 46 46 -
YRPVNREEAQSVKSNIVGM AER3_AERHY 46 46 -
YRPITREEAQSVKSNIVNM AER5_AERHY 46 46 -
YRPVNREEAQSIKSNIVGM AER4_AERHY 46 46 -
YRPINREEAQSVRNNIVGM P94128 46 46 -
YRPINREEAQSVRNNIVAM AERA_AERTR 46 46 -
YRPINREEAQSVRNNIVAM O85370 46 46 -
YRPLTRDEAMSIKGNLVSR AERA_AERSA 48 48 -
YRPINREEAQSVRNNIVAM AERA_AERSO 46 46 -

Motif 2 width=20
Element Seqn Id St Int Rpt
MGQWQISGLANGWVIMGPGY AERA_AERHY 65 0 -
MGQWQISGLANGWVIMGPGY Q44063 65 0 -
MGQWQISGLANGWVIMGPGY AER3_AERHY 65 0 -
MGQWQISGLANGWVIMGPVY AER5_AERHY 65 0 -
MGQWQISGLANGWVIMGPGY AER4_AERHY 65 0 -
MGQWQISGLANNWVIMGSGY P94128 65 0 -
MGQWQISGLANNWVILGPGY AERA_AERTR 65 0 -
MGQWQISGLANNWVILGPGY O85370 65 0 -
MGQWQITGLADRWVIMGPGY AERA_AERSA 67 0 -
MGQWQISGLANNWVILGPGY AERA_AERSO 65 0 -

Motif 3 width=21
Element Seqn Id St Int Rpt
NGEIKPGTASNTWCYPTNPVT AERA_AERHY 85 0 -
NGEIKPGSASNTWCYPVNPVT Q44063 85 0 -
NGEIKPGSASSTWCYPTNPAT AER3_AERHY 85 0 -
NGEIKPGSASNTWCYPVNPVT AER5_AERHY 85 0 -
NGEIKPGSASSTWCYPTNPAT AER4_AERHY 85 0 -
NGEIKPGSASDTWCYPTKPVT P94128 85 0 -
NGEIKPGKASTTWCYPTRPAT AERA_AERTR 85 0 -
NGEIKPGKASTTWCYPTRPAT O85370 85 0 -
NGEIKQGTAGETWCYPNSPVS AERA_AERSA 87 0 -
NGEIKPGKASTTWCYPTRPAT AERA_AERSO 85 0 -

Motif 4 width=23
Element Seqn Id St Int Rpt
SYLAHYLGYAWVGGNHSQYVGED AERA_AERHY 140 34 -
SYLAHYLGYAWVGGNHSQYVGED Q44063 140 34 -
SYLAHYLGYAWVGGNHSQYVGED AER3_AERHY 140 34 -
SYLAHYLGYAWVGGNHSQYVGED AER5_AERHY 140 34 -
SYLAHYLGYAWVGGNHSQYVGED AER4_AERHY 140 34 -
SYLAHYLGYAWVGGNHSPYVGED P94128 140 34 -
SYLAHYLGYAWVGGDHSQFVGDD AERA_AERTR 140 34 -
SYLAHYLGYAWVGGDHSQFVGDD O85370 140 34 -
SYLAHYLGYAWVGGNHSPYVGED AERA_AERSA 142 34 -
SYLAHYLGYAWVGGDHSQFVGDD AERA_AERSO 140 34 -

Motif 5 width=25
Element Seqn Id St Int Rpt
KNKFKWPLVGETELSIEIAANQSWA AERA_AERHY 265 102 -
KNKFKWPLVGETELSIEIAANQSWA Q44063 265 102 -
KNKFKWPLVGETELSIEIAANQSWA AER3_AERHY 265 102 -
KNKFKWPLVGETELSIEIAANQSWA AER5_AERHY 265 102 -
KNKFKWPLVGETELSIEIAANQSWA AER4_AERHY 265 102 -
KNKFKWPLVGETELSIEIAASQSWA P94128 265 102 -
KNKFKWPLVGETEVSIEIAANQSWA AERA_AERTR 265 102 -
KNKFKWPLVGETEVSIEIAANQSWA O85370 265 102 -
KNKFQWPLVGETELAIEIAASQSWA AERA_AERSA 266 101 -
KNKFKWPLVGETEVSIEIAANQSWA AERA_AERSO 265 102 -

Motif 6 width=24
Element Seqn Id St Int Rpt
YDLTLSGFLRWGGNAWYTHPDNRP AERA_AERHY 337 47 -
YDLTLSGFLRWGGNAWYTHPDNRP Q44063 337 47 -
YDLTLSGFLRWGGNAWYTHPDNRP AER3_AERHY 337 47 -
YDLTLSGFLRWGGNAWYTHPDNRP AER5_AERHY 337 47 -
YDLTLSGFLRWGGNAWYTHPDNRP AER4_AERHY 337 47 -
YDLTLNGFLRWGGNAWYTHPDNRP P94128 337 47 -
YDLTFNGFLRWGGNAWHTHPEDRP AERA_AERTR 337 47 -
YDLTFNGFLRWGGNAWHTHPEDRP O85370 337 47 -
YDLTMKGFLRWGGNAWYTHPDNRP AERA_AERSA 338 47 -
YDLTFNGFLRWGGNAWHTHPEDRP AERA_AERSO 337 47 -

Motif 7 width=20
Element Seqn Id St Int Rpt
WDKRYIPGEVKWWDWNWTIQ AERA_AERHY 382 21 -
WDKRYIPGEVKWWDWNWTIQ Q44063 382 21 -
WDKRYIPGEVKWWDWNWTIQ AER3_AERHY 382 21 -
WDKRYIPGEVKWWDWNWTIQ AER5_AERHY 382 21 -
WDKRYIPGEVKWWDWNWTIQ AER4_AERHY 382 21 -
WDKRYIPGEVKWWDWNWTIQ P94128 382 21 -
WDKRYLPGEMKWWDWNWAIQ AERA_AERTR 382 21 -
WDKRYLPGEMKWWDWNWAIQ O85370 382 21 -
VDKRYIPGEVKWWDWNWTIS AERA_AERSA 383 21 -
WDKRYLPGEMKWWDWNWAIQ AERA_AERSO 382 21 -

Motif 8 width=19
Element Seqn Id St Int Rpt
GLSTMQNNLARVLRPVRAG AERA_AERHY 404 2 -
GLSTMQNNLARVLRPVRAG Q44063 404 2 -
GLPTMQNNLAKVLRPVRAG AER3_AERHY 404 2 -
GLSTMQNNLARVLRPVRAG AER5_AERHY 404 2 -
GLPTMQNNLAKVLRPVRAG AER4_AERHY 404 2 -
GADTMKNALARVLRPVRAS P94128 404 2 -
GLATMQDSLARVLRPVRAS AERA_AERTR 404 2 -
GLATMQDSLARVLRPVRAS O85370 404 2 -
GLSTMQNNLGRVLRPIRSA AERA_AERSA 405 2 -
GLATMQDSLARVLRPVRAS AERA_AERSO 404 2 -

Motif 9 width=20
Element Seqn Id St Int Rpt
ITGDFSAESQFAGNIEIGAP AERA_AERHY 423 0 -
ITGDFSAESQFAGNIEIGAP Q44063 423 0 -
ITGDFSAESQFAGNIEIGAP AER3_AERHY 423 0 -
ITGDFSAESQFAGNIEIGAP AER5_AERHY 423 0 -
ITGDFSAESQFAGNIEIGAP AER4_AERHY 423 0 -
ITGDFHAESQFAGNIEIGAA P94128 423 0 -
ITGDFRAESQFAGNIEIGTP AERA_AERTR 423 0 -
ITGDFRAESQFAGNIEIGTP O85370 423 0 -
VTGDFYAESQFAGDIEIGQP AERA_AERSA 424 0 -
ITGDFRAESQFAGNIEIGTP AERA_AERSO 423 0 -