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PR00721

Identifier
STOMATIN  [View Relations]  [View Alignment]  
Accession
PR00721
No. of Motifs
7
Creation Date
07-JUN-1997  (UPDATE 19-JUN-1999)
Title
Stomatin signature
Database References

PROSITE; PS01270 BAND_7
PFAM; PF01145 Band_7
INTERPRO; IPR001972
Literature References
1. STEWART, G.W.
Stomatin.
INT.J.BIOCHEM.CELL BIOL. 29 271-274 (1997).
 
2. SCHLEGEL, W., UNFRIED, I. AND PROHASKA, R.
Cloning and analysis of a cDNA-encoding the BALB/C murine erythrocyte
band-7 integral membrane-protein.
GENE 178 115-118 (1996).
 
3. FOLEY, M. AND TILLEY, L.
Quinoline antimalarials: Mechanisms of action and resistance.
INT.J.PARASITOL. 27 231-240 (1997).

Documentation
Stomatin, also known as erythrocyte membrane protein band 7.2b, is a 31 kDa
membrane protein [1]. It was named after the rare human disease, haemolytic
anaemia hereditary stomatocytosis. The protein contains a single hydrophobic
domain, close to the N-terminus, and is phosphorylated [1]. The human gene
of stomatin is encoded by seven exons spread over 25 kb of genomic DNA [2].
 
Stomatin is believed to be involved in regulating monovalent cation
transport through lipid membranes. Absence of the protein in hereditary
stomatocytosis is believed to be the reason for the leakage of Na+ and K+
ions into and from erythrocytes [1].
 
A second function of stomatin is to act as a cytoskeletal anchor. One
possible example of this is its interaction with some anti-malarial drugs.
Current opinion speculates that such drugs bind to high density lipoproteins
in serum. The lipoproteins are delivered to erythrocytes, where it is
believed they interact with stomatin as a means of transfer to the
intracellular parasite, via a pathway used for the uptake of exogenous
phospholipid [3].
 
Stomatin-like proteins have been identified in various organisms, including
Caenorhabditis elegans and Mus musculus.
 
STOMATIN is a 7-element fingerprint that provides a signature for the
stomatins. The fingerprint was derived from an initial alignment of 10
sequences: the motifs were drawn from conserved regions spanning virtually
the full alignment length. A single iteration on OWL29.3 was required to
reach convergence, no further sequences being identified beyond the starting
set. Four partial matches were found, all of which are related sequences
that fail to make significant matches with one or more motifs.
 
An update on SPTR37_9f identified a true set of 14 sequences, and 13
partial matches.
Summary Information
  14 codes involving  7 elements
1 codes involving 6 elements
4 codes involving 5 elements
2 codes involving 4 elements
2 codes involving 3 elements
4 codes involving 2 elements
Composite Feature Index
714141414141414
60111111
53444140
40211220
30212010
23410000
1234567
True Positives
BAN7_HUMAN    BAN7_MOUSE    MEC2_CAEEL    O27808        
O28852 O59180 O67057 O69841
O88988 O93423 STO1_CAEEL STO2_CAEEL
STO3_CAEEL UNC1_CAEEL
True Positive Partials
Codes involving 6 elements
O50256
Codes involving 5 elements
O26788 O58205 Y827_METJA YS09_MYCTU
Codes involving 4 elements
O77376 YBBK_ECOLI
Codes involving 3 elements
O60121 YB28_SYNY3
Codes involving 2 elements
HFLK_ECOLI HFLK_HAEIN HFLK_VIBPA O60376
Sequence Titles
BAN7_HUMAN  ERYTHROCYTE BAND 7 INTEGRAL MEMBRANE PROTEIN (STOMATIN) (PROTEIN 7.2B) - HOMO SAPIENS (HUMAN). 
BAN7_MOUSE ERYTHROCYTE BAND 7 INTEGRAL MEMBRANE PROTEIN (STOMATIN) (PROTEIN 7.2B) - MUS MUSCULUS (MOUSE).
MEC2_CAEEL MECHANOSENSORY PROTEIN 2 - CAENORHABDITIS ELEGANS.
O27808 STOMATIN-LIKE PROTEIN - METHANOBACTERIUM THERMOAUTOTROPHICUM.
O28852 MEMBRANE PROTEIN - ARCHAEOGLOBUS FULGIDUS.
O59180 266AA LONG HYPOTHETICAL ERYTHROCYTE BAND7 INTEGRAL MEMBRANE PROTEIN - PYROCOCCUS HORIKOSHII.
O67057 ERYTHROCYTE BAND 7 HOMOLOG - AQUIFEX AEOLICUS.
O69841 HYPOTHETICAL 28.9 KD PROTEIN - STREPTOMYCES COELICOLOR.
O88988 STOMATIN - MUS MUSCULUS (MOUSE).
O93423 STOMATIN - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).
STO1_CAEEL STO-1 PROTEIN - CAENORHABDITIS ELEGANS.
STO2_CAEEL STO-2 PROTEIN - CAENORHABDITIS ELEGANS.
STO3_CAEEL STO-3 PROTEIN - CAENORHABDITIS ELEGANS.
UNC1_CAEEL UNC-1 PROTEIN - CAENORHABDITIS ELEGANS.

O50256 STOMATIN LIKE PROTEIN - RHIZOBIUM ETLI.

O26788 STOMATIN-LIKE PROTEIN - METHANOBACTERIUM THERMOAUTOTROPHICUM.
O58205 298AA LONG HYPOTHETICAL MEMBRANE PROTEIN - PYROCOCCUS HORIKOSHII.
Y827_METJA HYPOTHETICAL PROTEIN MJ0827 - METHANOCOCCUS JANNASCHII.
YS09_MYCTU HYPOTHETICAL 41.2 KD PROTEIN CY277.09 - MYCOBACTERIUM TUBERCULOSIS.

O77376 MAL3P6.19 PROTEIN - PLASMODIUM FALCIPARUM.
YBBK_ECOLI HYPOTHETICAL 33.7 KD PROTEIN IN USHA-TESA INTERGENIC REGION - ESCHERICHIA COLI.

O60121 HYPOTHETICAL 39.3 KD PROTEIN - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
YB28_SYNY3 HYPOTHETICAL 35.7 KD PROTEIN SLR1128 - SYNECHOCYSTIS SP. (STRAIN PCC 6803).

HFLK_ECOLI HFLK PROTEIN - ESCHERICHIA COLI.
HFLK_HAEIN HFLK PROTEIN - HAEMOPHILUS INFLUENZAE.
HFLK_VIBPA HFLK PROTEIN - VIBRIO PARAHAEMOLYTICUS.
O60376 P1.11659_4 - HOMO SAPIENS (HUMAN).
Scan History
OWL29_3    2  100  NSINGLE    
SPTR37_9f 2 55 NSINGLE
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
EYDRMVIFRLGRLWQDNPRGPGI CEF52D105 44 44 -
EYERAIIFRLGRILQGGAKGPGL BAN7_HUMAN 58 58 -
EYERVVIFRIGRLVFGGARGPGM CELK03E61 57 57 -
EYERAVIFRLGRLKHGGARGPGI CET04F87 12 12 -
EYERAVIFRLGRLMPGGAKGPGI S60260 146 146 -
EYERVIIFRLGRILQGGAKGPGV MMEMPEPB7 45 45 -
EYERVIIFRLGRILQGGAKGPGL MMU17297 59 59 -
EYERAVIFRLGRLIGGGAKGPGI CELF32A64 64 64 -
EYQRAVVFRLGRLVPDVKGPGIF CELF08C61 20 20 -
EYERVIIFRLGRILQGGAKGPGL BAN7_MOUSE 59 59 -

Motif 2 width=22
Element Seqn Id St Int Rpt
VLTKDSVTISVDGVVYYRVQNA MMU17297 108 26 -
VLTKDSVTISVDGVVYYRVQNA MMEMPEPB7 83 15 -
ILSKDSVTVAVDAVVYFRISNA S60260 195 26 -
ILTKDSVTTSVDAVIYYRISNA CELF32A64 113 26 -
ILSKDSVTVSVDAVIYFRISNA CET04F87 61 26 -
ILSKDSVTVSVDAVVYFRTSDP CELK03E61 106 26 -
MLTRDSVTIGVDAAVYYRTSDP CEF52D105 93 26 -
ILSRDSVTVSVDAVVYFKVFDP CELF08C61 68 25 -
ILTKDSVTISVDGVVYYRVQNA BAN7_HUMAN 107 26 -
VLTKDSVTISVDGVVYYRVQNA BAN7_MOUSE 108 26 -

Motif 3 width=18
Element Seqn Id St Int Rpt
TKLLAQTTLRNFLGTRTL CET04F87 96 13 -
TRLLAQTTLRNALGTKNL BAN7_MOUSE 143 13 -
TRLLAQTTLRNVLGTKNL BAN7_HUMAN 142 13 -
TKLLAQTTLRNILGTKTL S60260 230 13 -
TRLLAQTTLRNMLGTRSL CELF32A64 148 13 -
TRLLAQTTLRNALGTKNL MMU17297 143 13 -
TRLLAQTTLRNALGTKNL MMEMPEPB7 118 13 -
TKLLAQTTLRNALGMKTL CELK03E61 141 13 -
TRQLAQSSLRNVLGTRSL CEF52D105 128 13 -
TKLLAQTTLRTILGTHTL CELF08C61 103 13 -

Motif 4 width=24
Element Seqn Id St Int Rpt
LMTDRHGIAVQVKYILDSATLFWG CEF52D105 148 2 -
ILSDREKISADMKISLDEATEPWG CELF08C61 123 2 -
MLTEREAIAQLCETILDEGTEHWG CELK03E61 161 2 -
MLSSRDAISMQMQAALDEATDPWG CET04F87 116 2 -
ILSDREEIAHHMQSTLDDATDDWG MMEMPEPB7 138 2 -
ILSDREEIAHHMQSTLDDATDDWG MMU17297 163 2 -
ILSDRETLAASMQTILDEATESWG CELF32A64 168 2 -
MLSDREAISHQMQTTLDEATEPWG S60260 250 2 -
ILSDREEIAHHMQSTLDDATDDWG BAN7_MOUSE 163 2 -
ILSDREEIAHNMQSTLDDATDAWG BAN7_HUMAN 162 2 -

Motif 5 width=19
Element Seqn Id St Int Rpt
IKVERVEIKDVKLPVQLQR MMEMPEPB7 162 0 -
VKVERVEVKDVRLPVQLQR S60260 274 0 -
IKVERVEIKDVRLPIQLQR CELF32A64 192 0 -
IKVERVEIKDVKLPVQLQR MMU17297 187 0 -
VKVERVEIKDVRLPIQLQR CET04F87 140 0 -
VKVERVEVKDIRLPQQLTR CELK03E61 185 0 -
IHVERVEIKDIRLPREMCR CEF52D105 172 0 -
IKVERVELRDVRLPSQMQR CELF08C61 147 0 -
IKVERVEIKDVKLPVQLQR BAN7_HUMAN 186 0 -
IKVERVEIKDVKLPVQLQR BAN7_MOUSE 187 0 -

Motif 6 width=22
Element Seqn Id St Int Rpt
AMAAEAEAAREARAKVIAAEGE MMEMPEPB7 181 0 -
AMAAEAEAAREARAKVIAAEGE MMU17297 206 0 -
AMAAEAEATREARAKVIAAEGE CELF32A64 211 0 -
AMAAEAEAAREARAKVIVAEGE S60260 293 0 -
AMAAEAEATRDAGAKIIAAEGE CELF08C61 166 0 -
AMAAEAEAQRESDAKVVTAQGE CEF52D105 191 0 -
AMAAEAEASREARAKVIAAEGE BAN7_HUMAN 205 0 -
AMAAEAEAAREARAKVIAAEGE BAN7_MOUSE 206 0 -
AMAAEAEAAREARAKVVAAEGE CELK03E61 204 0 -
AMAAEAEAARAAGAKIIAAEGE CET04F87 159 0 -

Motif 7 width=24
Element Seqn Id St Int Rpt
SRALRDAASVIAQSPAALQLRYLQ CELF32A64 236 3 -
SRALKEASMVITESPAALQLRYLQ MMEMPEPB7 206 3 -
SRALADAADVIATSPCAIQLRYLQ CET04F87 184 3 -
SRALKEAADVIQANPVALQLRHLQ CELK03E61 229 3 -
SMAFQKAADELAGSPTALQLRYLQ CEF52D105 216 3 -
SAALAEAATIISKSEGAMQLRYLH CELF08C61 191 3 -
SRALKEASMVITESPAALQLRYLQ BAN7_HUMAN 230 3 -
SRALKEASMVITESPAALQLRYLQ BAN7_MOUSE 231 3 -
SRALKEAAEVIAESPSALQLRYLQ S60260 318 3 -
SRALKEASMVITESPAALQLRYLQ MMU17297 231 3 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
EYERAIIFRLGRILQGGAKGPGL BAN7_HUMAN 58 58 -
EYERAIIFRLGRILRGGAKGPGL O93423 61 61 -
EYERVIIFRLGRILQGGAKGPGL BAN7_MOUSE 59 59 -
EYERVIIFRLGRILQGGAKGPGL O88988 59 59 -
EYERAVIFRLGRLMPGGAKGPGI MEC2_CAEEL 146 146 -
EYERAVIFRLGRLIGGGAKGPGI STO2_CAEEL 64 64 -
EYERVVIFRIGRLVFGGARGPGM UNC1_CAEEL 57 57 -
EYQRAVVFRLGRLVPDVKGPGIF STO1_CAEEL 20 20 -
EYERGVIFRLGRLVGARGPGLFF O28852 25 25 -
EYERAVIFRLGRVVGARGPGLFF O59180 30 30 -
EYERAVVFRLGRVIGAKGPGLII O67057 28 28 -
EYDRMVIFRLGRLWQDNPRGPGI STO3_CAEEL 44 44 -
QYERGVVFRLGKVIGVREPGLRI O27808 69 69 -
QYERGVVFRLGRLAGQARGPGFT O69841 29 29 -

Motif 2 width=22
Element Seqn Id St Int Rpt
ILTKDSVTISVDGVVYYRVQNA BAN7_HUMAN 107 26 -
VLTKDSVTVSVDGVVYYRVQNA O93423 110 26 -
VLTKDSVTISVDGVVYYRVQNA BAN7_MOUSE 108 26 -
VLTKDSVTISVDGVVYYRVQNA O88988 108 26 -
ILSKDSVTVAVDAVVYFRISNA MEC2_CAEEL 195 26 -
ILTKDSVTTSVDAVIYYRISNA STO2_CAEEL 113 26 -
ILSKDSVTVSVDAVVYFRTSDP UNC1_CAEEL 106 26 -
ILSRDSVTVSVDAVVYFKVFDP STO1_CAEEL 68 25 -
VVTKDNVTVKVNAVVYYRVVDP O28852 72 24 -
TITKDNVPVRVNAVVYFRVVDP O59180 77 24 -
VITKDNVTVQVDAVVYFRVVDP O67057 75 24 -
MLTRDSVTIGVDAAVYYRTSDP STO3_CAEEL 93 26 -
IITQDNVSIDVAAVAYFKVADP O27808 116 24 -
GITRDNVTVRVDAVVYFKVVDA O69841 77 25 -

Motif 3 width=18
Element Seqn Id St Int Rpt
TRLLAQTTLRNVLGTKNL BAN7_HUMAN 142 13 -
TRLLAQTTLRNVLGTKNL O93423 145 13 -
TRLLAQTTLRNALGTKNL BAN7_MOUSE 143 13 -
TRLLAQTTLRNALGTKNL O88988 143 13 -
TKLLAQTTLRNILGTKTL MEC2_CAEEL 230 13 -
TRLLAQTTLRNMLGTRSL STO2_CAEEL 148 13 -
TKLLAQTTLRNALGMKTL UNC1_CAEEL 141 13 -
TKLLAQTTLRTILGTHTL STO1_CAEEL 103 13 -
TAQLAQTTLRSIIGQAEL O28852 107 13 -
TSQISQTTLRSVIGQAHL O59180 112 13 -
TSQIAQTTLRSVCGEAEL O67057 110 13 -
TRQLAQSSLRNVLGTRSL STO3_CAEEL 128 13 -
VNQISQTTVRNVIGQFVL O27808 151 13 -
VSQMAQTSLRSIIGKSDL O69841 112 13 -

Motif 4 width=24
Element Seqn Id St Int Rpt
ILSDREEIAHNMQSTLDDATDAWG BAN7_HUMAN 162 2 -
ILSDREEIAHSMQSTLDDATDDWG O93423 165 2 -
ILSDREEIAHHMQSTLDDATDDWG BAN7_MOUSE 163 2 -
ILSDREEIAHHMQSTLDDATDDWG O88988 163 2 -
MLSDREAISHQMQTTLDEATEPWG MEC2_CAEEL 250 2 -
ILSDRETLAASMQTILDEATESWG STO2_CAEEL 168 2 -
MLTEREAIAQLCETILDEGTEHWG UNC1_CAEEL 161 2 -
ILSDREKISADMKISLDEATEPWG STO1_CAEEL 123 2 -
VLSERDKLNVKLQQIIDEETNPWG O28852 127 2 -
LLSERDKLNMQLQRIIDEATDPWG O59180 132 2 -
LLSQREKINMKLQEIIDRQTDPWG O67057 130 2 -
LMTDRHGIAVQVKYILDSATLFWG STO3_CAEEL 148 2 -
VLSETARINEKIKEIIDEHSEPWG O27808 171 2 -
LLSDREKLNQGLELMIDSPAVGWG O69841 132 2 -

Motif 5 width=19
Element Seqn Id St Int Rpt
IKVERVEIKDVKLPVQLQR BAN7_HUMAN 186 0 -
IKVERVEIKDVKLPLQLQR O93423 189 0 -
IKVERVEIKDVKLPVQLQR BAN7_MOUSE 187 0 -
IKVERVEIKDVKLPVQLQR O88988 187 0 -
VKVERVEVKDVRLPVQLQR MEC2_CAEEL 274 0 -
IKVERVEIKDVRLPIQLQR STO2_CAEEL 192 0 -
VKVERVEVKDIRLPQQLTR UNC1_CAEEL 185 0 -
IKVERVELRDVRLPSQMQR STO1_CAEEL 147 0 -
IKVTAVEIKDVELPEEMRR O28852 151 0 -
IKVTAVEIKDVELPAGMQK O59180 156 0 -
VKVIAVELKKIDLPEELRK O67057 154 0 -
IHVERVEIKDIRLPREMCR STO3_CAEEL 172 0 -
INVTTVEIKDIKLPEGMQR O27808 195 0 -
VQIDRVEIKDVSLPDTMKR O69841 156 0 -

Motif 6 width=22
Element Seqn Id St Int Rpt
AMAAEAEASREARAKVIAAEGE BAN7_HUMAN 205 0 -
AMAAEAEASREARAKVIAAEGE O93423 208 0 -
AMAAEAEAAREARAKVIAAEGE BAN7_MOUSE 206 0 -
AMAAEAEAAREARAKVIAAEGE O88988 206 0 -
AMAAEAEAAREARAKVIVAEGE MEC2_CAEEL 293 0 -
AMAAEAEATREARAKVIAAEGE STO2_CAEEL 211 0 -
AMAAEAEAAREARAKVVAAEGE UNC1_CAEEL 204 0 -
AMAAEAEATRDAGAKIIAAEGE STO1_CAEEL 166 0 -
IMAMQAEAERERRSKIIRAEGE O28852 170 0 -
AMARQAEAERERRARITLAEAE O59180 175 0 -
ALARQAEAERERRAKIISAEAE O67057 173 0 -
AMAAEAEAQRESDAKVVTAQGE STO3_CAEEL 191 0 -
AMARQAEAERDKRAKIITAEGE O27808 214 0 -
SMARQAEADRERRARVINADAE O69841 175 0 -

Motif 7 width=24
Element Seqn Id St Int Rpt
SRALKEASMVITESPAALQLRYLQ BAN7_HUMAN 230 3 -
SRALKEASLVIAESPSALQLRYLQ O93423 233 3 -
SRALKEASMVITESPAALQLRYLQ BAN7_MOUSE 231 3 -
SRALKEASMVITESPAALQLRYLQ O88988 231 3 -
SRALKEAAEVIAESPSALQLRYLQ MEC2_CAEEL 318 3 -
SRALRDAASVIAQSPAALQLRYLQ STO2_CAEEL 236 3 -
SRALKEAADVIQANPVALQLRHLQ UNC1_CAEEL 229 3 -
SAALAEAATIISKSEGAMQLRYLH STO1_CAEEL 191 3 -
AMKLREAADVLAQSEGAILLRYLQ O28852 195 3 -
AEKLREAAEIISEHPMALQLRTLQ O59180 200 3 -
AQKLLEAARILAQEPIAIQLRYLE O67057 198 3 -
SMAFQKAADELAGSPTALQLRYLQ STO3_CAEEL 216 3 -
AAKLGEAADVIEKHPVALQLRNLQ O27808 239 3 -
SKVLAEAAREMSETPAALQLRLLQ O69841 200 3 -