| Literature References | 1. VOURIO, T., MAITY, S.N. AND DE CROMBRUGGHE, B.
Purification and molecular cloning of the "A" chain of a rat heterotrimeric
CCAAT-binding protein. Sequence identity with the yeast HAP3 transcription
factor.
J.BIOL.CHEM. 265 22480-22486 (1990).
2. LI, X-Y, MANTOVANI, R., HOOFT VAN HUIJSDUIJNEN, R., ANDRE, I.,
BENOIST, C. AND MATHIS, D.
Evolutionary variation of the CCAAT-binding transcription factor NF-Y.
NUCLEIC ACIDS RES. 20 1087-1091 (1992); ERRATUM: NUCLEIC ACIDS RES. 20 1841 (1992).
3. MAITY, S.N. AND DE CROMBRUGGHE, B.
Biochemical analysis of the B subunit of the heteromeric CCAAT-binding
factor. A DNA-binding domain and a subunit interaction domain are specified
by two separate segments.
J.BIOL.CHEM. 267 8286-8292 (1992).
4. BECKER, D.M., FIKES, J.D. AND GUARENTE, L.
A cDNA encoding a human CCAAT-binding protein cloned by functional
complementation in yeast.
PROC.NATL.ACAD.SCI.U.S.A. 88 1968-1972 (1991).
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| Documentation | The CCAAT-binding factor (CBF) is a mammalian transcription factor that
binds to a CCAAT motif in the promoters of a wide variety of genes,
including type I collagen and albumin [1,2]. The factor is a heteromeric
complex of A and B subunits, both of which are required for DNA-binding
[1,2]. The subunits can interact in the absence of DNA-binding, conserved
regions in each being important in mediating this interaction.
The B subunit contains a region of 65 amino acids that shares 75% identity
with the yeast protein HAP2 [4]. Deletions in the C-terminus of this region
results in the loss of DNA-binding ability, but subunit interaction is
unaffected [3]. It is therefore suggested that the N-terminal portion of
the conserved region is involved in subunit interaction and the C-terminal
region involved in DNA-binding [3].
CCAATSUBUNTB is a 2-element fingerprint that provides a signature for the
CCAAT-binding transcription factor B subunit. The fingerprint was derived
from an initial alignment of 6 sequences: the motifs were drawn from
virtually the full length of the central conserved region - motif 1 spans
the subunit-association domain encoded by PROSITE pattern CBFB_NFYA
(PS00686); and motif 2 encodes the DNA recognition domain. Two iterations
on OWL28.2 were required to reach convergence, at which point a true set
comprising 11 sequences was identified.
An update on SPTR37_9f identified a true set of 18 sequences.
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