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PR00616

Identifier
CCAATSUBUNTB  [View Relations]  [View Alignment]  
Accession
PR00616
No. of Motifs
2
Creation Date
01-NOV-1996  (UPDATE 25-JUN-1999)
Title
CCAAT-binding transcription factor subunit B signature
Database References

PROSITE; PS00686 CBFB_NFYA
BLOCKS; BL00686
INTERPRO; IPR001289
Literature References
1. VOURIO, T., MAITY, S.N. AND DE CROMBRUGGHE, B.
Purification and molecular cloning of the "A" chain of a rat heterotrimeric
CCAAT-binding protein. Sequence identity with the yeast HAP3 transcription
factor.
J.BIOL.CHEM. 265 22480-22486 (1990).
 
2. LI, X-Y, MANTOVANI, R., HOOFT VAN HUIJSDUIJNEN, R., ANDRE, I.,
BENOIST, C. AND MATHIS, D.
Evolutionary variation of the CCAAT-binding transcription factor NF-Y.
NUCLEIC ACIDS RES. 20 1087-1091 (1992); ERRATUM: NUCLEIC ACIDS RES. 20 1841 (1992).
 
3. MAITY, S.N. AND DE CROMBRUGGHE, B.
Biochemical analysis of the B subunit of the heteromeric CCAAT-binding 
factor. A DNA-binding domain and a subunit interaction domain are specified
by two separate segments.
J.BIOL.CHEM. 267 8286-8292 (1992).
 
4. BECKER, D.M., FIKES, J.D. AND GUARENTE, L.
A cDNA encoding a human CCAAT-binding protein cloned by functional
complementation in yeast.
PROC.NATL.ACAD.SCI.U.S.A. 88 1968-1972 (1991).

Documentation
The CCAAT-binding factor (CBF) is a mammalian transcription factor that
binds to a CCAAT motif in the promoters of a wide variety of genes,
including type I collagen and albumin [1,2]. The factor is a heteromeric
complex of A and B subunits, both of which are required for DNA-binding
[1,2]. The subunits can interact in the absence of DNA-binding, conserved
regions in each being important in mediating this interaction.
 
The B subunit contains a region of 65 amino acids that shares 75% identity
with the yeast protein HAP2 [4]. Deletions in the C-terminus of this region
results in the loss of DNA-binding ability, but subunit interaction is
unaffected [3]. It is therefore suggested that the N-terminal portion of
the conserved region is involved in subunit interaction and the C-terminal
region involved in DNA-binding [3].
 
CCAATSUBUNTB is a 2-element fingerprint that provides a signature for the
CCAAT-binding transcription factor B subunit. The fingerprint was derived
from an initial alignment of 6 sequences: the motifs were drawn from 
virtually the full length of the central conserved region - motif 1 spans 
the subunit-association domain encoded by PROSITE pattern CBFB_NFYA
(PS00686); and motif 2 encodes the DNA recognition domain. Two iterations
on OWL28.2 were required to reach convergence, at which point a true set
comprising 11 sequences was identified.
 
An update on SPTR37_9f identified a true set of 18 sequences.
Summary Information
18 codes involving  2 elements
Composite Feature Index
21818
12
True Positives
CBFB_HUMAN    CBFB_MOUSE    CBFB_RAT      HAP2_KLULA    
HAP2_YEAST O23630 O23631 O23632
O49915 O59847 O64585 O73743
P87249 PHP2_SCHPO Q26561 Q26652
Q42471 Q42508
Sequence Titles
CBFB_HUMAN  CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT B (CBF-B) (NF-Y PROTEIN CHAIN A) (NF-YA) (CAAT-BOX DNA BINDING PROTEIN SUBUNIT A) - HOMO SAPIENS (HUMAN). 
CBFB_MOUSE CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT B (CBF-B) (NF-Y PROTEIN CHAIN A) (NF-YA) (CAAT-BOX DNA BINDING PROTEIN SUBUNIT A) - MUS MUSCULUS (MOUSE).
CBFB_RAT CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT B (CBF-B) (NF-Y PROTEIN CHAIN A) (NF-YA) (CAAT-BOX DNA BINDING PROTEIN SUBUNIT A) - RATTUS NORVEGICUS (RAT).
HAP2_KLULA HAP2 TRANSCRIPTIONAL ACTIVATOR - KLUYVEROMYCES LACTIS (YEAST).
HAP2_YEAST HAP2 TRANSCRIPTIONAL ACTIVATOR - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
O23630 TRANSCRIPTION FACTOR - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O23631 TRANSCRIPTION FACTOR - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O23632 TRANSCRIPTION FACTOR - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O49915 RAPB PROTEIN - ORYZA SATIVA (RICE).
O59847 HAPB - ASPERGILLUS ORYZAE.
O64585 SIMILAR TO RAPB TRANSCRIPTION FACTOR - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O73743 NUCLEAR Y/CCAAT-BOX BINDING FACTOR A SUBUNIT NF-YA - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
P87249 HAPB PROTEIN - EMERICELLA NIDULANS (ASPERGILLUS NIDULANS).
PHP2_SCHPO PHP2 TRANSCRIPTIONAL ACTIVATOR - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
Q26561 NF-YA SUBUNIT - SCHISTOSOMA MANSONI (BLOOD FLUKE).
Q26652 NF-Y-A SUBUNIT - STRONGYLOCENTROTUS PURPURATUS (PURPLE SEA URCHIN).
Q42471 CCAAT-BINDING FACTOR B SUBUNIT HOMOLOG - BRASSICA NAPUS (RAPE).
Q42508 CCAAT-BINDING FACTOR B SUBUNIT HOMOLOG - BRASSICA NAPUS (RAPE).
Scan History
OWL26_2    2  100  NSINGLE    
SPTR37_9f 2 100 NSINGLE
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
PLYVNAKQYHRILKRRQARAKLE CBFB_HUMAN 264 264 -
PLYVNAKQYHRILKRRQARAKLE CBFB_RAT 258 258 -
PLYVNAKQYHRILKRRQARAKLE CBFB_MOUSE 263 263 -
PFYVNAKQYYRILKRRYARAKLE HAP2_YEAST 160 160 -
GLYVNAKQYHRILKRREARAKLE PHP2_SCHPO 9 9 -

Motif 2 width=24
Element Seqn Id St Int Rpt
RRKYLHESRHRHAMARKRGEGGRF CBFB_HUMAN 295 8 -
RRKYLHESRHRHAMARKRGEGGRF CBFB_RAT 289 8 -
RRKYLHESRHRHAMARKRGEGGRF CBFB_MOUSE 294 8 -
RKPYLHESRHKHAMRRPRGEGGRF HAP2_YEAST 191 8 -
KKPYLHESRHKHAMRRPRGPGGRF PHP2_SCHPO 41 9 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
PLYVNAKQYHRILKRRQARAKLE CBFB_HUMAN 264 264 -
PLYVNAKQYHRILKRRQARAKLE CBFB_RAT 258 258 -
PLYVNAKQYHRILKRRQARAKLE CBFB_MOUSE 263 263 -
PLYVNAKQYHRILKRRQARAKLE O73743 227 227 -
PLYVNAKQYHRILKRRQARAKLE Q26652 308 308 -
PFYVNAKQYYRILKRRYARAKLE HAP2_YEAST 160 160 -
PFYVNAKQYYRILKRRYARAKLE HAP2_KLULA 186 186 -
PLYVNAKQYHRILKRRQARAKLE Q26561 174 174 -
GLYVNAKQYHRILKRREARAKLE PHP2_SCHPO 9 9 -
PLYVNAKQFHRILKRRVARQKLE P87249 213 213 -
PLYVNAKQFHRILKRRVARQKLE O59847 234 234 -
PIYVNAKQYHAILRRRQLRAKLE O49915 139 139 -
PVYVNAKQYQAIMRRRQARAKAE Q42471 178 178 -
PVYVNAKQYQAIMRRRQARAKAE Q42508 167 167 -
PVYVNAKQYEGILRRRKARAKAE O23630 172 172 -
PVFVNAKQYHAIMRRRQQRAKLE O23632 180 180 -
PVFVNAKQYHGILRRRQSRAKLE O64585 127 127 -
TIYVNSKQYHGIIRRRQSRAKAA O23631 137 137 -

Motif 2 width=24
Element Seqn Id St Int Rpt
RRKYLHESRHRHAMARKRGEGGRF CBFB_HUMAN 295 8 -
RRKYLHESRHRHAMARKRGEGGRF CBFB_RAT 289 8 -
RRKYLHESRHRHAMARKRGEGGRF CBFB_MOUSE 294 8 -
RRKYLHESRHRHAMARKRGDGGRF O73743 258 8 -
RRKYLHESRHNHAMNRVRGEGGRF Q26652 339 8 -
RKPYLHESRHKHAMRRPRGEGGRF HAP2_YEAST 191 8 -
RRPYLHESRHKHAMRRPRGQGGRF HAP2_KLULA 217 8 -
RRKYLHESRHKHAMNRIRSSGGRF Q26561 205 8 -
KKPYLHESRHKHAMRRPRGPGGRF PHP2_SCHPO 41 9 -
RKPYLHESRHNHAMRRPRGPGGRF P87249 245 9 -
RKPYLHESRHNHAMRRPRGPGGRF O59847 266 9 -
RKPYLHESRHQHAMKRARGTGGRF O49915 170 8 -
RKRYLHESRHQHAMRRPRGTGGRF Q42471 209 8 -
RKRYLHESRHQHAMRRPRGTGGRF Q42508 198 8 -
RKPYLHESRHKHAMRRARASGGRF O23630 203 8 -
RKPYLHESRHVHALKRPRGSGGRF O23632 211 8 -
KKPYMHESRHLHAIRRPRGCGGRF O64585 158 8 -
RKPYMHHSRHLHALRRPRGSGGRF O23631 172 12 -