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PR00491

Identifier
VASOACTVEIPR  [View Relations]  [View Alignment]  
Accession
PR00491
No. of Motifs
4
Creation Date
03-MAR-1996  (UPDATE 07-JUN-1999)
Title
Vasoactive intestinal peptide receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00249 GPCRSECRETIN
PRINTS; PR01156 PACAPRECEPTR; PR01154 VIP1RECEPTOR; PR01155 VIP2RECEPTOR

INTERPRO; IPR001571
GCRDB; GCR_0397; GCR_0652; GCR_0774
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.   
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.  
Molecular cloning and expression of a cDNA encoding the secretin receptor.
EMBO J. 10 1635-1641 (1991).
 
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H.,
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor.
SCIENCE 254 1022-1024 (1991). 
 
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F.,
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
 
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
 
6. WATSON, S. AND ARKINSTALL, S.
Vasoactive intestinal polypeptide family.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.278-283.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that   
encompasses a wide range of functions (including various autocrine, para-   
crine and endocrine processes). They show considerable diversity at the   
sequence level, on the basis of which they can be separated into distinct   
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,   
but between which there is no statistically significant similarity in   
sequence [1]. The currently known clan members include the rhodopsin-like   
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating   
pheromone receptors, and the metabotropic glutamate receptor family.       
 
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid   
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-   
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a manner
reminiscent of the rhodopsins and other receptors believed to interact with
G proteins. However, while a similar 3D framework has been proposed to
account for this, there is no significant sequence identity between these
families: the secretin-like receptors thus bear their own unique `7TM'
signature.      
 
Vasoactive intestinal polypeptide (VIP) has a wide physiological profile.
In the periphery, it induces relaxation in smooth muscle; inhibits
secretion in certain tissues, but stimulates secretion in others; and
modulates activity of cells in the immune system [6]. In the CNS, it has a
range of both excitatory and inhibitory actions. VIP receptors are
distributed widely in the periphery, and occur throughout the gastro-
intestinal tract and genitourinary system, other smooth muscles and
secretory glands. In the CNS, they are found abundantly in, e.g. the cortex,
hippocampus and thalamus [6]. All VIP receptors activate adenylyl cyclase.
 
VASOACTVEIPR is a 4-element fingerprint that provides a signature for the
vasoactive intestinal peptide receptors. The fingerprint was derived from
an initial alignment of 5 sequences: the motifs were drawn from conserved
sections within the N- and C-termini, focusing on those areas of the
alignment that characterise the VIP receptors but distinguish them from the
rest of the secretin-like family - motifs 1-3 lie in the N-terminal region
preceding the first TM domain, and motif 4 lies at the C-terminus. Two
iterations on OWL27.0 were required to reach convergence, at which point a
true set comprising 6 sequences was identified. Seven partial matches were
also found, all of which are members of the secretin-like family.
 
An update on SPTR37_9f identified a true set of 6 sequences.
Summary Information
6 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
46666
30000
20000
1234
True Positives
VIPR_HUMAN    VIPR_PIG      VIPR_RAT      VIPS_HUMAN    
VIPS_MOUSE VIPS_RAT
Sequence Titles
VIPR_HUMAN  VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-R-1) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE II RECEPTOR) (PACAP TYPE II RECEPTOR) (PACAP-R-2) - HOMO SAPIENS (HUMAN). 
VIPR_PIG VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-R-1) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE II RECEPTOR) (PACAP TYPE II RECEPTOR) (PACAP-R-2) - SUS SCROFA (PIG).
VIPR_RAT VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-R-1) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE II RECEPTOR) (PACAP TYPE II RECEPTOR) (PACAP-R-2) - RATTUS NORVEGICUS (RAT).
VIPS_HUMAN VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-R-2) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE III RECEPTOR) (PACAP TYPE III RECEPTOR) (PACAP-R-3) (HELODERMIN-PREFERRING VIP RECEPTOR) - HOMO SAPIENS (HUMAN).
VIPS_MOUSE VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-R-2) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE III RECEPTOR) (PACAP TYPE III RECEPTOR) (PACAP-R-3) - MUS MUSCULUS (MOUSE).
VIPS_RAT VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-R-2) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE III RECEPTOR) (PACAP TYPE III RECEPTOR) (PACAP-R-3) - RATTUS NORVEGICUS (RAT).
Scan History
OWL27_0    2  100  NSINGLE    
SPTR37_9f 2 7 NSINGLE
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
PKVFSNFYSRPG VIPS_MOUSE 75 75 -
PKVFSNFYSRPG VIPS_RAT 75 75 -
PKVFSNFYSKAG VIPS_HUMAN 76 76 -
PLIFKLFSSIQG VIPR_HUMAN 87 87 -
PLIFQLFAPIHG VIPR_RAT 87 87 -

Motif 2 width=16
Element Seqn Id St Int Rpt
NISKNCTSDGWSETFP VIPS_MOUSE 87 0 -
NISKNCTSDGWSETFP VIPS_RAT 87 0 -
NISKNCTSDGWSETFP VIPS_HUMAN 88 0 -
NVSRSCTDEGWTHLEP VIPR_HUMAN 100 1 -
NISRSCTEEGWSQLEP VIPR_RAT 100 1 -

Motif 3 width=11
Element Seqn Id St Int Rpt
DFIDACGYNDP VIPS_MOUSE 103 0 -
DFIDACGYNDP VIPS_RAT 103 0 -
DFVDACGYSDP VIPS_HUMAN 104 0 -
PYPIACGLDDK VIPR_HUMAN 117 1 -
PYHIACGLNDR VIPR_RAT 117 1 -

Motif 4 width=12
Element Seqn Id St Int Rpt
GSRTQSFLQSET VIPS_MOUSE 423 309 -
GSRTQSFLQSET VIPS_RAT 423 309 -
ASRAQSFLQTET VIPS_HUMAN 424 309 -
ARRSSSFQAEVS VIPR_HUMAN 444 316 -
ARRSSSFQAEVS VIPR_RAT 446 318 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
PKVFSNFYSRPG VIPS_MOUSE 75 75 -
PKVFSNFYSRPG VIPS_RAT 75 75 -
PKVFSNFYSKAG VIPS_HUMAN 76 76 -
PLIFKLFSSIQG VIPR_HUMAN 87 87 -
PLIFKLFSPTQG VIPR_PIG 88 88 -
PLIFQLFAPIHG VIPR_RAT 87 87 -

Motif 2 width=16
Element Seqn Id St Int Rpt
NISKNCTSDGWSETFP VIPS_MOUSE 87 0 -
NISKNCTSDGWSETFP VIPS_RAT 87 0 -
NISKNCTSDGWSETFP VIPS_HUMAN 88 0 -
NVSRSCTDEGWTHLEP VIPR_HUMAN 100 1 -
NVSRNCTDEGWTPLEP VIPR_PIG 101 1 -
NISRSCTEEGWSQLEP VIPR_RAT 100 1 -

Motif 3 width=11
Element Seqn Id St Int Rpt
DFIDACGYNDP VIPS_MOUSE 103 0 -
DFIDACGYNDP VIPS_RAT 103 0 -
DFVDACGYSDP VIPS_HUMAN 104 0 -
PYPIACGLDDK VIPR_HUMAN 117 1 -
PYPIACGMDDK VIPR_PIG 118 1 -
PYHIACGLNDR VIPR_RAT 117 1 -

Motif 4 width=12
Element Seqn Id St Int Rpt
GSRTQSFLQSET VIPS_MOUSE 423 309 -
GSRTQSFLQSET VIPS_RAT 423 309 -
ASRAQSFLQTET VIPS_HUMAN 424 309 -
ARRSSSFQAEVS VIPR_HUMAN 444 316 -
ARRSSSFQAEVS VIPR_PIG 445 316 -
ARRSSSFQAEVS VIPR_RAT 446 318 -