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PR00428

Identifier
PROSTAGLNDNR  [View Relations]  [View Alignment]  
Accession
PR00428
No. of Motifs
3
Creation Date
20-NOV-1995  (UPDATE 23-JUN-1999)
Title
Prostaglandin receptor signature 
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01788 PROSTANOIDR; PR00854 PRSTNOIDDPR; PR00580 PRSTNOIDEP1R
PRINTS; PR00581 PRSTNOIDEP2R; PR00582 PRSTNOIDEP3R; PR00586 PRSTNOIDEP4R
PRINTS; PR00855 PRSTNOIDFPR; PR00856 PRSTNOIDIPR
INTERPRO; IPR001244
GCRDB; GCR_0367; GCR_0549
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL,
Prostanoids.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, pp239-251.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Prostanoids (prostaglandins (PG) and thromboxanes (TX)) mediate a wide
variety of actions and play important physiological roles in the cardio-
vascular and immune systems, and in pain sensation in peripheral systems
[6]. PGI2 and TXA2 have opposing actions, involving regulation of the
interaction of platelets with the vascular endothelium, while PGE2, PGI2
and PGD2 are powerful vasodilators and potentiate the action of various
autocoids to induce plasma extravasation and pain sensation. To date,
evidence for at least 5 classes of prostanoid receptor has been obtained.
However, identification of subtypes and their distribution is hampered by
expression of more than one receptor within a tissue, coupled with poor
selectivity of available agonists and antagonists. 
 
PROSTAGLNDNR is a 3-element fingerprint that provides a signature for the
prostaglandin receptors. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the prostaglandin receptors but distinguish them from the
rest of the rhodopsin-like superfamily - the motifs were drawn from TM
domains 1, 2 and 5 respectively. Two iterations on OWL26.2 were required
to reach convergence, at which point a true set comprising 28 sequences
was identified.
 
An update on SPTR37_9f identified a true set of 23 sequences, and 3
partial matches.
Summary Information
23 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3232323
2000
123
True Positives
O00325        O00326        O15188        O15189        
O15190 O15191 O15192 O15193
O15549 O46657 PE21_HUMAN PE21_MOUSE
PE21_RAT PE23_BOVIN PE23_HUMAN PE23_MOUSE
PE23_PIG PE23_RABIT PE23_RAT PE24_HUMAN
PE24_MOUSE PE24_RABIT PE24_RAT
Sequence Titles
O00325      PROSTAGLANDIN EP3 RECEPTOR SUBTYPE ISOFORM - Homo sapiens (Human). 
O00326 PROSTAGLANDIN EP3 RECEPTOR SUBTYPE ISOFORM - Homo sapiens (Human).
O15188 PROSTAGLANDIN EP3 RECEPTOR - HOMO SAPIENS (HUMAN).
O15189 PROSTAGLANDIN EP3 RECEPTOR - HOMO SAPIENS (HUMAN).
O15190 PROSTAGLANDIN EP3 RECEPTOR - HOMO SAPIENS (HUMAN).
O15191 PROSTAGLANDIN EP3 RECEPTOR - Homo sapiens (Human).
O15192 PROSTAGLANDIN EP3 RECEPTOR - HOMO SAPIENS (HUMAN).
O15193 PROSTAGLANDIN EP3 RECEPTOR - HOMO SAPIENS (HUMAN).
O15549 PROSTAGLANDIN EP3 RECEPTOR - HOMO SAPIENS (HUMAN).
O46657 PROSTAGLANDIN EP3 RECEPTOR - Sus scrofa (Pig).
PE21_HUMAN Prostaglandin E2 receptor, EP1 subtype (Prostanoid EP1 receptor) (PGE receptor, EP1 subtype) - Homo sapiens (Human).
PE21_MOUSE Prostaglandin E2 receptor, EP1 subtype (Prostanoid EP1 receptor) (PGE receptor, EP1 subtype) - Mus musculus (Mouse).
PE21_RAT Prostaglandin E2 receptor, EP1 subtype (Prostanoid EP1 receptor) (PGE receptor, EP1 subtype) - Rattus norvegicus (Rat).
PE23_BOVIN Prostaglandin E2 receptor, EP3 subtype (Prostanoid EP3 receptor) (PGE receptor, EP3 subtype) - Bos taurus (Bovine).
PE23_HUMAN Prostaglandin E2 receptor, EP3 subtype (Prostanoid EP3 receptor) (PGE receptor, EP3 subtype) - Homo sapiens (Human).
PE23_MOUSE Prostaglandin E2 receptor, EP3 subtype (Prostanoid EP3 receptor) (PGE receptor, EP3 subtype) - Mus musculus (Mouse).
PE23_PIG Prostaglandin E2 receptor, EP3 subtype (Prostanoid EP3 receptor) (PGE receptor, EP3 subtype) - Sus scrofa (Pig).
PE23_RABIT Prostaglandin E2 receptor, EP3 subtype (Prostanoid EP3 receptor) (PGE receptor, EP3 subtype) - Oryctolagus cuniculus (Rabbit).
PE23_RAT Prostaglandin E2 receptor, EP3 subtype (Prostanoid EP3 receptor) (PGE receptor, EP3 subtype) - Rattus norvegicus (Rat).
PE24_HUMAN Prostaglandin E2 receptor, EP4 subtype (Prostanoid EP4 receptor) (PGE receptor, EP4 subtype) - Homo sapiens (Human).
PE24_MOUSE Prostaglandin E2 receptor, EP4 subtype (Prostanoid EP4 receptor) (PGE receptor, EP4 subtype) - Mus musculus (Mouse).
PE24_RABIT Prostaglandin E2 receptor, EP4 subtype (Prostanoid EP4 receptor) (PGE receptor, subtype EP4) - Oryctolagus cuniculus (Rabbit).
PE24_RAT Prostaglandin E2 receptor, EP4 subtype (Prostanoid EP4 receptor) (PGE receptor, EP4 subtype) - Rattus norvegicus (Rat).
Scan History
OWL26_2    2  200  NSINGLE    
SPTR37_9f 2 100 NSINGLE
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
AFPITMMVTGFV O70361 30 30 -
AFPITMMVTGFV PER3_RAT 30 30 -
AFSMTMMITGFV PER3_BOVIN 52 52 -
ALPIFSMTLGAV PER1_HUMAN 37 37 -
ALPIFSMTLGAV PER1_MOUSE 39 39 -
TIPAVMFIFGVV O15055 22 22 -
TIPAVMFIFGVV O54943 47 47 -

Motif 2 width=13
Element Seqn Id St Int Rpt
GQLLTSPVVILVY O70361 79 37 -
GQLLTSPVVILVY PER3_RAT 79 37 -
GQLLTSPVVIVLY PER3_BOVIN 101 37 -
GHVIPGALVLRLY PER1_HUMAN 87 38 -
GHVIPGALVLRLY PER1_MOUSE 89 38 -
GTLLVSPVTIATY O15055 68 34 -
GTLLVSPVTIATY O54943 93 34 -

Motif 3 width=14
Element Seqn Id St Int Rpt
ACLGLLALVVTFAC O70361 211 119 -
ACLGLLALVVTFAC PER3_RAT 211 119 -
AILGLSALVVTFAC PER3_BOVIN 234 120 -
ASLGLVALLAALVC PER1_HUMAN 208 108 -
AGLGLAALLAALVC PER1_MOUSE 210 108 -
AGFSSFLILATVLC O15055 189 108 -
AGFSSFLILATVLC O54943 214 108 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
AFPITMMVTGFV PE23_MOUSE 30 30 -
AFPITMMVTGFV PE23_RAT 30 30 -
AFPITMLLTGFV PE23_HUMAN 53 53 -
AFPITMLLTGFV O15549 53 53 -
AFPITMLLTGFV O15193 53 53 -
AFPITMLLTGFV O15192 53 53 -
AFPITMLLTGFV O15191 53 53 -
AFPITMLLTGFV O15190 53 53 -
AFPITMLLTGFV O15189 53 53 -
AFPITMLLTGFV O15188 53 53 -
AFPITMLLTGFV O00326 53 53 -
AFPITMLLTGFV O00325 53 53 -
AFPITMLITGFV PE23_RABIT 49 49 -
AFSMTMMITGFV PE23_BOVIN 52 52 -
VFPMTMLITGFV PE23_PIG 30 30 -
VFPMTMLITGFV O46657 52 52 -
TIPAVMFIFGVV PE24_HUMAN 22 22 -
TIPAVMFIFGVV PE24_RAT 22 22 -
TIPAVMFIFGVV PE24_RABIT 22 22 -
TIPAVMFIFGVV PE24_MOUSE 47 47 -
ALPIFSMTLGAV PE21_MOUSE 39 39 -
ALPIFSMTLGAV PE21_RAT 39 39 -
ALPIFSMTLGAV PE21_HUMAN 37 37 -

Motif 2 width=13
Element Seqn Id St Int Rpt
GQLLTSPVVILVY PE23_MOUSE 79 37 -
GQLLTSPVVILVY PE23_RAT 79 37 -
GQLLTTPVVIVVY PE23_HUMAN 102 37 -
GQLLTTPVVIVVY O15549 102 37 -
GQLLTTPVVIVVY O15193 102 37 -
GQLLTTPVVIVVY O15192 102 37 -
GQLLTTPVVIVVY O15191 102 37 -
GQLLTTPVVIVVY O15190 102 37 -
GQLLTTPVVIVVY O15189 102 37 -
GQLLTTPVVIVVY O15188 102 37 -
GQLLTTPVVIVVY O00326 102 37 -
GQLLTTPVVIVVY O00325 102 37 -
GQLLTSPVVILVY PE23_RABIT 98 37 -
GQLLTSPVVIVLY PE23_BOVIN 101 37 -
GQLLTSPVVIVLY PE23_PIG 79 37 -
GQLLTSPVVIVLY O46657 101 37 -
GTLLVSPVTIATY PE24_HUMAN 68 34 -
GTLLVSPVTIATY PE24_RAT 68 34 -
GTLLVSPVTIATY PE24_RABIT 68 34 -
GTLLVSPVTIATY PE24_MOUSE 93 34 -
GHVIPGALVLRLY PE21_MOUSE 89 38 -
GHVIPGALVLRLY PE21_RAT 89 38 -
GHVIPGALVLRLY PE21_HUMAN 87 38 -

Motif 3 width=14
Element Seqn Id St Int Rpt
ACLGLLALVVTFAC PE23_MOUSE 211 119 -
ACLGLLALVVTFAC PE23_RAT 211 119 -
AFLGLLALTVTFSC PE23_HUMAN 235 120 -
AFLGLLALTVTFSC O15549 235 120 -
AFLGLLALTVTFSC O15193 235 120 -
AFLGLLALTVTFSC O15192 235 120 -
AFLGLLALTVTFSC O15191 235 120 -
AFLGLLALTVTFSC O15190 235 120 -
AFLGLLALTVTFSC O15189 235 120 -
AFLGLLALTVTFSC O15188 235 120 -
AFLGLLALTVTFSC O00326 235 120 -
AFLGLLALTVTFSC O00325 235 120 -
AFLGLLALAITFTC PE23_RABIT 231 120 -
AILGLSALVVTFAC PE23_BOVIN 234 120 -
SFLGLSALVVTFAC PE23_PIG 212 120 -
SFLGLSALVVTFAC O46657 234 120 -
AGFSSFLILATVLC PE24_HUMAN 189 108 -
AGFSSFLILATVLC PE24_RAT 189 108 -
AGFSSFLILATVLC PE24_RABIT 189 108 -
AGFSSFLILATVLC PE24_MOUSE 214 108 -
AGLGLAALLAALVC PE21_MOUSE 210 108 -
AGLGLAALLAALVC PE21_RAT 210 108 -
ASLGLVALLAALVC PE21_HUMAN 208 108 -