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PR00381

Identifier
KINESINLIGHT  [View Relations]  [View Alignment]  
Accession
PR00381
No. of Motifs
6
Creation Date
15-APR-1995  (UPDATE 07-JUN-1999)
Title
Kinesin light chain signature
Database References

PROSITE; PS01160 KINESIN_LIGHT
PFAM; PF00409 kinesin2
INTERPRO; IPR002151
Literature References
1. BLOOM, G. AND ENDOW, S.
Motor proteins 1: kinesins.
PROTEIN PROFILE 1(10) 1059-1116 (1994).
 
2. AMOS, L.A.
Kinesin from pig brain studied by electron microscopy.
J.CELL SCI. 87 105-111 (1987).

Documentation
Kinesins are found in organisms from all the major eukaryotic kingdoms:
protists, fungi, plants and animals [1]. They are believed to function
as motors for microtubule-dependent motile phenomena, such as membrane
transport, and chromosome movement during mitosis and meiosis. The
common feature of kinesins is a highly-conserved `motor domain' that
contains binding sites for ATP and microtubules. Linked to the motor
domains are `tail domains' that vary somewhat in amino acid sequence.
It is believed that tail domains may specify the type of cargo that
particular kinesins are able to transport along microtubules.
 
Kinesins contain 2 heavy and 2 light chains. Maintenance of their
quaternary structure does not require interchain disulphide bonds.
Details of the structure have been revealed by high-resolution electron
microscopy [2], showing kinesin to be an asymmetric rod-like molecule
(~80nm long), with a pair of globular heads located at one end and a
fan-shaped tail at the other. The light chains and C-termini of the heavy
chains form the tail domains, which associate with membrane-bound
organelles. Most kinesin light chains range in predicted size from 58 to
65kDa and are mildly acidic. The sequences share from 60-90% identity,
most of the diversity residing in the C-termini. This suggests that these
regions, at least in part, are responsible for their transport specificity. 
 
KINESINLIGHT is a 6-element fingerprint that provides a signature for
kinesin light chains. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved regions
spanning the central portion of the alignment. Two iterations on OWL25.2
were required to reach convergence, at which point a true set comprising
11 sequences was identified.
 
An update on SPTR37_9f identified a true set of 10 sequences, and 1
partial match.
Summary Information
  10 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6101010101010
5000000
4000000
3000111
2000000
123456
True Positives
KNLC_CAEEL    KNLC_DROME    KNLC_HUMAN    KNLC_LOLPE    
KNLC_RAT KNLC_STRPU O88447 O88448
Q21592 Q90707
True Positive Partials
Codes involving 3 elements
O33965
Sequence Titles
KNLC_CAEEL  KINESIN LIGHT CHAIN (KLC) - CAENORHABDITIS ELEGANS. 
KNLC_DROME KINESIN LIGHT CHAIN (KLC) - DROSOPHILA MELANOGASTER (FRUIT FLY).
KNLC_HUMAN KINESIN LIGHT CHAIN (KLC) - HOMO SAPIENS (HUMAN).
KNLC_LOLPE KINESIN LIGHT CHAIN (KLC) - LOLIGO PEALEII (LONGFIN SQUID).
KNLC_RAT KINESIN LIGHT CHAIN (KLC) - RATTUS NORVEGICUS (RAT).
KNLC_STRPU KINESIN LIGHT CHAIN (KLC) - STRONGYLOCENTROTUS PURPURATUS (PURPLE SEA URCHIN).
O88447 KINESIN LIGHT CHAIN 1 - MUS MUSCULUS (MOUSE).
O88448 KINESIN LIGHT CHAIN 2 - MUS MUSCULUS (MOUSE).
Q21592 M7.2 PROTEIN - CAENORHABDITIS ELEGANS.
Q90707 KINESIN LIGHT CHAIN (KLC) - GALLUS GALLUS (CHICKEN).

O33965 KINESIN LIGHT CHAIN - PLECTONEMA BORYANUM.
Scan History
OWL25_2    2  50   NSINGLE    
SPTR37_9f 2 62 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
AQVRRLVQENTWLRDELA KNLC_STRPU 110 110 -
AQVRRLCQENQWLRDELA KNLC_RAT 104 104 -
AQVRRLCQENQWLRDELA KNLC_HUMAN 104 104 -
AQRRRLCQENAWLRDELS S41864 92 92 -
AQVRRLCQENAWLRDELA SQDSKLC 114 114 -

Motif 2 width=20
Element Seqn Id St Int Rpt
GYEIPARLRTLHNLVIQYAS KNLC_HUMAN 202 80 -
GCEIPARLRTLHNLVIQYAS SQDSKLC 213 81 -
GYEIPARLRTLHNLVIQYAS S41864 199 89 -
GYEIPARLRTLHNLVIQYAS KNLC_RAT 202 80 -
GYEIPARLRTLHNLVIQYAS KNLC_STRPU 208 80 -

Motif 3 width=19
Element Seqn Id St Int Rpt
RYEVAVPLCKQALEDLEKT KNLC_RAT 224 2 -
RYEVAVPLCKQALEDLEKT S41864 221 2 -
RYEVAVPLCKQALEDLEKT SQDSKLC 235 2 -
RYEVAVPLCKQALEDLEKT KNLC_HUMAN 224 2 -
RYEVAVPLCKQALEDLEKT KNLC_STRPU 230 2 -

Motif 4 width=18
Element Seqn Id St Int Rpt
HPAVAATLNNLAVLYGKR KNLC_HUMAN 289 46 -
HPAVAATLNNLAVLYGKR KNLC_STRPU 295 46 -
HPAVAATLNNLAVLFGKR S41864 286 46 -
HPAVAATLNNLAVLYGKR SQDSKLC 300 46 -
HPAVAATLNNLAVLYGKR KNLC_RAT 289 46 -

Motif 5 width=21
Element Seqn Id St Int Rpt
DVAKQLNNLALLCQNQGKYEE S41864 330 26 -
DVAKQLNNLALLCQNQGKYEE KNLC_HUMAN 333 26 -
DVAKQLNNLALLCQNQGKYEE KNLC_RAT 333 26 -
DVAKQLNNLALLCQNQGKYEE KNLC_STRPU 339 26 -
DVAKQLNNLALLCQNQGKYEE SQDSKLC 344 26 -

Motif 6 width=22
Element Seqn Id St Int Rpt
KLGPDDPNVAKTKNNLASCYLK KNLC_HUMAN 368 14 -
KLGPDDPNVAKTKNNLAAAYLK KNLC_STRPU 374 14 -
KLGPDDPNVAKTKNNLASCYLK KNLC_RAT 368 14 -
KLGPDDPNVAKTKNNLSSAYLK S41864 365 14 -
ELGPDDPNVAKTKNNLASAYLK SQDSKLC 379 14 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
AQVRRLCQENQWLRDELA KNLC_HUMAN 104 104 -
AQVRRLCQENQWLRDELA Q90707 108 108 -
AQVRRLCQENQWLRDELA KNLC_RAT 104 104 -
AQVRRLVQENTWLRDELA KNLC_STRPU 110 110 -
AQVRRLCQENAWLRDELA KNLC_LOLPE 114 114 -
AQVRRLVQENQWLREELA O88448 97 97 -
AQRRRLCQENAWLRDELS KNLC_CAEEL 92 92 -
TQVRRLHQENAWLRDELA KNLC_DROME 83 83 -
AQVRRLCQENQWLRDELA O88447 108 108 -
ESIRLLRQENTWLLDELT Q21592 97 97 -

Motif 2 width=20
Element Seqn Id St Int Rpt
GYEIPARLRTLHNLVIQYAS KNLC_HUMAN 202 80 -
GYEIPARLRTLHNLVIQYAS Q90707 164 38 -
GYEIPARLRTLHNLVIQYAS KNLC_RAT 202 80 -
GYEIPARLRTLHNLVIQYAS KNLC_STRPU 208 80 -
GCEIPARLRTLHNLVIQYAS KNLC_LOLPE 213 81 -
GYEIPARLRTLHNLVIQYAS O88448 190 75 -
GYEIPARLRTLHNLVIQYAS KNLC_CAEEL 199 89 -
GYEIPARLRTLHNLVIQYAS KNLC_DROME 179 78 -
GYEIPARLRTLHNLVIQYAS O88447 204 78 -
DYQLPTRLQTLQNLVIQYME Q21592 199 84 -

Motif 3 width=19
Element Seqn Id St Int Rpt
RYEVAVPLCKQALEDLEKT KNLC_HUMAN 224 2 -
RYEVAVPLCKQALEDLEKT Q90707 186 2 -
RYEVAVPLCKQALEDLEKT KNLC_RAT 224 2 -
RYEVAVPLCKQALEDLEKT KNLC_STRPU 230 2 -
RYEVAVPLCKQALEDLEKT KNLC_LOLPE 235 2 -
RYEVAVPLCKQALEDLEKT O88448 212 2 -
RYEVAVPLCKQALEDLEKT KNLC_CAEEL 221 2 -
RYEVAVPLCKQALEDLERT KNLC_DROME 201 2 -
RYEVAVPSCKQALEDLEKT O88447 226 2 -
RFEVAIPLCKQALEDVVKV Q21592 221 2 -

Motif 4 width=18
Element Seqn Id St Int Rpt
HPAVAATLNNLAVLYGKR KNLC_HUMAN 289 46 -
HPAVAATLNNLAVLYGKR Q90707 251 46 -
HPAVAATLNNLAVLYGKR KNLC_RAT 289 46 -
HPAVAATLNNLAVLYGKR KNLC_STRPU 295 46 -
HPAVAATLNNLAVLYGKR KNLC_LOLPE 300 46 -
HPAVAATLNNLAVLYGKR O88448 277 46 -
HPAVAATLNNLAVLFGKR KNLC_CAEEL 286 46 -
HPAVAATLNNLAVLYGKR KNLC_DROME 266 46 -
HPAVAATLNNLAVLYGKR O88447 291 46 -
HHSVAATLNNLAIAYGKR Q21592 286 46 -

Motif 5 width=21
Element Seqn Id St Int Rpt
DVAKQLNNLALLCQNQGKYEE KNLC_HUMAN 333 26 -
DVAKQLNNLALLCQNQGKYEE Q90707 295 26 -
DVAKQLNNLALLCQNQGKYEE KNLC_RAT 333 26 -
DVAKQLNNLALLCQNQGKYEE KNLC_STRPU 339 26 -
DVAKQLNNLALLCQNQGKYEE KNLC_LOLPE 344 26 -
DVAKQLSNLALLCQNQGKAEE O88448 321 26 -
DVAKQLNNLALLCQNQGKYEE KNLC_CAEEL 330 26 -
DVAKQLNNLALLCQNQGKYDE KNLC_DROME 310 26 -
DVAKQLNNLALLCQNQGKYEE O88447 335 26 -
DVAKQLTNLGIVTQQLEKYEE Q21592 330 26 -

Motif 6 width=22
Element Seqn Id St Int Rpt
KLGPDDPNVAKTKNNLASCYLK KNLC_HUMAN 368 14 -
KLGPDDPNVAKTKNNLASCYLK Q90707 330 14 -
KLGPDDPNVAKTKNNLASCYLK KNLC_RAT 368 14 -
KLGPDDPNVAKTKNNLAAAYLK KNLC_STRPU 374 14 -
ELGPDDPNVAKTKNNLASAYLK KNLC_LOLPE 379 14 -
RLGPDDPNVAKTKNNLASCYLK O88448 356 14 -
KLGPDDPNVAKTKNNLSSAYLK KNLC_CAEEL 365 14 -
KLGPDDPNVAKTKNNLAGCYLK KNLC_DROME 345 14 -
LGPDRTPNVAKTKNNLASCYLK O88447 371 15 -
AFPENHQNVIKTKNQLASVFLK Q21592 365 14 -