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PR00372

Identifier
FYWHYDRXLASE  [View Relations]  [View Alignment]  
Accession
PR00372
No. of Motifs
6
Creation Date
11-MAY-1995  (UPDATE 07-JUN-1999)
Title
Biopterin-dependent aromatic amino acid hydroxylase signature
Database References

PROSITE; PS00367 BIOPTERIN_HYDROXYL
BLOCKS; BL00367
PFAM; PF00351 biopterin_H
INTERPRO; IPR001273
Literature References
1. DAUBNER, S.C. AND PIPER, M.
Deletion mutants of tyrosine hydroxylase identify a region critical
for heparin binding.
PROTEIN SCI. 4 538-541 (1995).

Documentation
Phenylalanine- , tyrosine- and tryptophan hydroxylases constitute a family
of tetrahydrobiopterin-dependent aromatic amino acid hydroxylases, all of
which are rate-limiting catalysts for important metabolic pathways [1]. The
proteins are structurally and functionally related, each containing iron,
and catalysing ring hydroxylation of aromatic amino acids, using tetra-
hydrobiopterin (BH4) as a substrate. All are regulated by phosphorylation
at serines in their N-termini.
 
It has been suggested that the proteins each contain a conserved C-terminal
catalytic (C) domain and an unrelated N-terminal regulatory (R) domain.
It is possible that the R domains arose from genes that were recruited
from different sources to combine with the common gene for the catalytic
core. Thus, by combining with the same C domain, the proteins acquired
the unique regulatory properties of the separate R domains.   
 
FYWHYDRXLASE is a 6-element fingerprint that provides a signature for
aromatic amino acid hydroxylases. The fingerprint was derived from an
initial alignment of 6 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length (the region encoded
by PROSITE pattern BIOPTERIN_HYDROXYL (PS00367), which contains 2 conserved
histidines, lies between motifs 2 and 3). Two iterations on OWL26.0 were
required to reach convergence, at which point a true set comprising 21
sequences was identified. 
 
An update on SPTR37_9f identified a true set of 23 sequences, and 2
partial matches.
Summary Information
  23 codes involving  6 elements
2 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6232323232323
5222121
4000000
3000000
2000000
123456
True Positives
PH4H_CAEEL    PH4H_DROME    PH4H_HUMAN    PH4H_MOUSE    
PH4H_RAT Q23438 Q24000 Q27599
Q27600 TR5H_CHICK TR5H_HUMAN TR5H_MOUSE
TR5H_RABIT TR5H_RAT TR5H_XENLA TY3H_ANGAN
TY3H_BOVIN TY3H_CAEEL TY3H_DROME TY3H_HUMAN
TY3H_MOUSE TY3H_PHASP TY3H_RAT
True Positive Partials
Codes involving 5 elements
O17498 TY3H_SCHMA
Sequence Titles
PH4H_CAEEL  PUTATIVE PHENYLALANINE-4-HYDROXYLASE (EC 1.14.16.1) (PAH) (PHE-4- MONOOXYGENASE) - CAENORHABDITIS ELEGANS. 
PH4H_DROME HENNA PROTEIN [INCLUDES: PHENYLALANINE-4-HYDROXYLASE (EC 1.14.16.1) (PAH) (PHE-4-MONOOXYGENASE); TRYPTOPHAN 5-MONOOXYGENASE (EC 1.14.16.4) (TRH) (TRYPTOPHAN 5-HYDROXYLASE)] - DROSOPHILA MELANOGASTER (FRUIT FLY).
PH4H_HUMAN PHENYLALANINE-4-HYDROXYLASE (EC 1.14.16.1) (PAH) (PHE-4- MONOOXYGENASE) - HOMO SAPIENS (HUMAN).
PH4H_MOUSE PHENYLALANINE-4-HYDROXYLASE (EC 1.14.16.1) (PAH) (PHE-4- MONOOXYGENASE) - MUS MUSCULUS (MOUSE).
PH4H_RAT PHENYLALANINE-4-HYDROXYLASE (EC 1.14.16.1) (PAH) (PHE-4- MONOOXYGENASE) - RATTUS NORVEGICUS (RAT).
Q23438 COSMID ZK1290 - CAENORHABDITIS ELEGANS.
Q24000 TYROSINE HYDROXYLASE TYPE 2, HYPODERMAL FORM - DROSOPHILA MELANOGASTER (FRUIT FLY).
Q27599 PHENYLALANINE HYDROXYLASE (EC 1.14.16.1) (PHENYLALANINE 4-MONOOXYGENASE) (PHENYLALANINE 4-HYDROXYLASE) (PHENYLALANINASE) - DROSOPHILA MELANOGASTER (FRUIT FLY).
Q27600 PHENYLALANINE HYDROXYLASE (EC 1.14.16.1) (PHENYLALANINE 4-MONOOXYGENASE) (PHENYLALANINE 4-HYDROXYLASE) (PHENYLALANINASE) - DROSOPHILA MELANOGASTER (FRUIT FLY).
TR5H_CHICK TRYPTOPHAN 5-MONOOXYGENASE (EC 1.14.16.4) (TRYPTOPHAN 5-HYDROXYLASE) - GALLUS GALLUS (CHICKEN).
TR5H_HUMAN TRYPTOPHAN 5-MONOOXYGENASE (EC 1.14.16.4) (TRYPTOPHAN 5-HYDROXYLASE) - HOMO SAPIENS (HUMAN).
TR5H_MOUSE TRYPTOPHAN 5-MONOOXYGENASE (EC 1.14.16.4) (TRYPTOPHAN 5-HYDROXYLASE) - MUS MUSCULUS (MOUSE).
TR5H_RABIT TRYPTOPHAN 5-MONOOXYGENASE (EC 1.14.16.4) (TRYPTOPHAN 5-HYDROXYLASE) - ORYCTOLAGUS CUNICULUS (RABBIT).
TR5H_RAT TRYPTOPHAN 5-MONOOXYGENASE (EC 1.14.16.4) (TRYPTOPHAN 5-HYDROXYLASE) - RATTUS NORVEGICUS (RAT).
TR5H_XENLA TRYPTOPHAN 5-MONOOXYGENASE (EC 1.14.16.4) (TRYPTOPHAN 5-HYDROXYLASE) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
TY3H_ANGAN TYROSINE 3-MONOOXYGENASE (EC 1.14.16.2) (TYROSINE 3-HYDROXYLASE) (TH) - ANGUILLA ANGUILLA (EUROPEAN FRESHWATER EEL).
TY3H_BOVIN TYROSINE 3-MONOOXYGENASE (EC 1.14.16.2) (TYROSINE 3-HYDROXYLASE) (TH) - BOS TAURUS (BOVINE).
TY3H_CAEEL PUTATIVE TYROSINE 3-MONOOXYGENASE (EC 1.14.16.2) (TYROSINE 3- HYDROXYLASE) (TH) - CAENORHABDITIS ELEGANS.
TY3H_DROME TYROSINE 3-MONOOXYGENASE (EC 1.14.16.2) (TYROSINE 3-HYDROXYLASE) (TH) (PROTEIN PALE) - DROSOPHILA MELANOGASTER (FRUIT FLY).
TY3H_HUMAN TYROSINE 3-MONOOXYGENASE (EC 1.14.16.2) (TYROSINE 3-HYDROXYLASE) (TH) - HOMO SAPIENS (HUMAN).
TY3H_MOUSE TYROSINE 3-MONOOXYGENASE (EC 1.14.16.2) (TYROSINE 3-HYDROXYLASE) (TH) - MUS MUSCULUS (MOUSE).
TY3H_PHASP TYROSINE 3-MONOOXYGENASE (EC 1.14.16.2) (TYROSINE 3-HYDROXYLASE) (TH) - PHASIANIDAE SP. (QUAIL).
TY3H_RAT TYROSINE 3-MONOOXYGENASE (EC 1.14.16.2) (TYROSINE 3-HYDROXYLASE) (TH) - RATTUS NORVEGICUS (RAT).

O17498 PHENYLALANINE HYDROXYLASE (EC 1.14.16.1) - BRANCHIOSTOMA FLORIDAE (FLORIDA LANCELET) (AMPHIOXUS).
TY3H_SCHMA TYROSINE 3-MONOOXYGENASE (EC 1.14.16.2) (TYROSINE 3-HYDROXYLASE) (TH) - SCHISTOSOMA MANSONI (BLOOD FLUKE).
Scan History
OWL26_0    2  100  NSINGLE    
SPTR37_9f 2 25 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
PGFSDQVYRQRRKLIAEIAF TY3H_RAT 193 193 -
PGFSDQAYRQRRKLIAEIAF TY3H_MOUSE 193 193 -
PGFSDQVYRQRRKLIAEIAF TY3H_HUMAN 223 223 -
PGFSDQAYRQRRKLIAEIAF TY3H_BOVIN 186 186 -
PGYSDQVYRQRRKSIAEIAF TY3H_PHASP 186 186 -
PGFADKVYRQRRKEIAEIAF TY3H_DROME 200 200 -

Motif 2 width=23
Element Seqn Id St Int Rpt
TGFQLRPVAGLLSARDFLASLAF TY3H_RAT 284 71 -
TGFQLRPVAGLLSARDFLASLAF TY3H_MOUSE 284 71 -
TGFQLRPVAGLLSARDFLASLAF TY3H_HUMAN 314 71 -
TGFQLRPVAGLLSARDFLASLAF TY3H_BOVIN 277 71 -
TGFQLRPVRGLLSARDFLASLAF TY3H_PHASP 277 71 -
TGFSLRPAAGLLTARDFLASLAF TY3H_DROME 291 71 -

Motif 3 width=20
Element Seqn Id St Int Rpt
FSQDIGLASLGASDEEIEKL TY3H_RAT 348 41 -
FSQDIGLASLGASDEEIEKL TY3H_MOUSE 348 41 -
FSQDIGLASLGASDEEIEKL TY3H_HUMAN 378 41 -
FSQDIGLASLGVSDEEIEKL TY3H_BOVIN 341 41 -
FSQDIGLASLGATDEEIEKL TY3H_PHASP 341 41 -
FSQEIGLASLGASDEEIEKL TY3H_DROME 355 41 -

Motif 4 width=19
Element Seqn Id St Int Rpt
AYGAGLLSSYGELLHSLSE TY3H_RAT 388 20 -
AYGAGLLSSYGELLHSLSE TY3H_MOUSE 388 20 -
AYGAGLLSSYGELLHCLSE TY3H_HUMAN 418 20 -
AYGAGLLSSYGELLHSLSE TY3H_BOVIN 381 20 -
AYGAGLLSSYGELIHSLSD TY3H_PHASP 381 20 -
AYGAGLLSSYGELLHAISD TY3H_DROME 395 20 -

Motif 5 width=20
Element Seqn Id St Int Rpt
AVQPYQDQTYQPVYFVSESF TY3H_RAT 419 12 -
AVQPYQDQTYQPVYFVSESF TY3H_MOUSE 419 12 -
AVQPYQDQTYQSVYFVSESF TY3H_HUMAN 449 12 -
AVQPYQDQTYQPVYFVSESF TY3H_BOVIN 412 12 -
AVQPCQDQPYQPVYFVSESF TY3H_PHASP 412 12 -
AVQPYQDQEYQPIYYVAESF TY3H_DROME 426 12 -

Motif 6 width=19
Element Seqn Id St Int Rpt
RPFSVKFDPYTLAIDVLDS TY3H_RAT 454 15 -
RPFSVKFDPYTLAIDVLDS TY3H_MOUSE 454 15 -
RPFSVKFDPYTLAIDVLDS TY3H_HUMAN 484 15 -
RPFSVKFDPYTLAIDVLDS TY3H_BOVIN 447 15 -
RPFSVKYEPYTHSIELLDS TY3H_PHASP 447 15 -
RPFEVRFNPHTERVEVLDS TY3H_DROME 461 15 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
PGFSDQVYRQRRKLIAEIAF TY3H_RAT 193 193 -
PGFSDQAYRQRRKLIAEIAF TY3H_MOUSE 193 193 -
PGFSDQVYRQRRKLIAEIAF TY3H_HUMAN 223 223 -
PGFKDPVYRARRKQFADIAY PH4H_RAT 147 147 -
PGFKDPVYRARRKQFADIAY PH4H_HUMAN 147 147 -
PGFSDQAYRQRRKLIAEIAF TY3H_BOVIN 186 186 -
PGFKDPVYRARRKQFADIAY PH4H_MOUSE 147 147 -
PGYSDQVYRQRRKSIAEIAF TY3H_PHASP 186 186 -
PGFKDNVYRKRRKYFADLAM TR5H_CHICK 135 135 -
PGFTDPEYRKRRKYFADIAY PH4H_DROME 146 146 -
PGFTDPEYRKRRKYFADIAY Q27599 146 146 -
PGFTDPEYRKRRKYFADIAY Q27600 141 141 -
PGFADKVYRQRRKEIAEIAF Q24000 271 271 -
PGFADKVYRQRRKEIAEIAF TY3H_DROME 200 200 -
PGFTDPVYRQRRRMIGEIAF TY3H_ANGAN 183 183 -
PGFKDNVYRRRRKYFAELAM TR5H_RAT 134 134 -
PGFKDNVYRKRRKYFADLAM TR5H_HUMAN 134 134 -
PGFKDNVYRRRRKYFAELAM TR5H_MOUSE 137 137 -
PGFKDNVYRKRRKYFADLAM TR5H_RABIT 134 134 -
PGFKDNVYRKRRKYFADVAM TR5H_XENLA 171 171 -
PGFKDMTYRERRKFFADIAF PH4H_CAEEL 147 147 -
PGFKDTEYRQRRMMFAELAL Q23438 257 257 -
PGHGDVAYIARRKFLNDQAL TY3H_CAEEL 94 94 -

Motif 2 width=23
Element Seqn Id St Int Rpt
TGFQLRPVAGLLSARDFLASLAF TY3H_RAT 284 71 -
TGFQLRPVAGLLSARDFLASLAF TY3H_MOUSE 284 71 -
TGFQLRPVAGLLSARDFLASLAF TY3H_HUMAN 314 71 -
TGFRLRPVAGLLSSRDFLGGLAF PH4H_RAT 238 71 -
TGFRLRPVAGLLSSRDFLGGLAF PH4H_HUMAN 238 71 -
TGFQLRPVAGLLSARDFLASLAF TY3H_BOVIN 277 71 -
TGFRLRPVAGLLSSRDFLGGLAF PH4H_MOUSE 238 71 -
TGFQLRPVRGLLSARDFLASLAF TY3H_PHASP 277 71 -
TGFTIRPVAGYLSPRDFLAGLAF TR5H_CHICK 226 71 -
TGFTLRPVAGLLSSRDFLAGLAF PH4H_DROME 237 71 -
TGFTLRPVAGLLSSRDFLAGLAF Q27599 237 71 -
TGFTLRPVAGLLSSRDFLAGLAF Q27600 232 71 -
TGFSLRPAAGLLTARDFLASLAF Q24000 362 71 -
TGFSLRPAAGLLTARDFLASLAF TY3H_DROME 291 71 -
TGFQLRPVAGLLSARDFLASLAF TY3H_ANGAN 274 71 -
TGFSIRPVAGYLSPRDFLSGLAF TR5H_RAT 225 71 -
TGFSIRPVAGYLSPRDFLSGLAF TR5H_HUMAN 225 71 -
TGFSIRPVAGYLSPRDFLSGLAF TR5H_MOUSE 228 71 -
TGFSIRPVAGYLSPRDFLSGLAF TR5H_RABIT 225 71 -
TGFTIRPVAGYLSPRDFLAGLAF TR5H_XENLA 262 71 -
TGYTIRPVAGLLSSRDFLAGLAF PH4H_CAEEL 238 71 -
TGFRVRPVAGYLSARDFLAGLAY Q23438 348 71 -
TGFELRPCSGLLSARDFLASLAF TY3H_CAEEL 185 71 -

Motif 3 width=20
Element Seqn Id St Int Rpt
FSQDIGLASLGASDEEIEKL TY3H_RAT 348 41 -
FSQDIGLASLGASDEEIEKL TY3H_MOUSE 348 41 -
FSQDIGLASLGASDEEIEKL TY3H_HUMAN 378 41 -
FSQEIGLASLGAPDEYIEKL PH4H_RAT 302 41 -
FSQEIGLASLGAPDEYIEKL PH4H_HUMAN 302 41 -
FSQDIGLASLGVSDEEIEKL TY3H_BOVIN 341 41 -
FSQEIGLASLGAPDEYIEKL PH4H_MOUSE 302 41 -
FSQDIGLASLGATDEEIEKL TY3H_PHASP 341 41 -
FSQEIGLASLGASDEAVQKL TR5H_CHICK 290 41 -
FSQEIGLASLGAPDDYIEKL PH4H_DROME 301 41 -
FSQEIGLASLGAPDDYIEKL Q27599 301 41 -
FSQEIGLASLGAPDDYIEKL Q27600 296 41 -
FSQEIGLASLGASDEEIEKL Q24000 426 41 -
FSQEIGLASLGASDEEIEKL TY3H_DROME 355 41 -
FSQNIGLASLGASEEDIEKL TY3H_ANGAN 338 41 -
FSQEIGLASLGASEETVQKL TR5H_RAT 289 41 -
FSQEIGLASLGASEEAVQKL TR5H_HUMAN 289 41 -
FSQEIGLASLGASEETVQKL TR5H_MOUSE 292 41 -
FSQEIGLASLGASEEAVQKL TR5H_RABIT 289 41 -
FSQEIGLASLGASDEAVQKL TR5H_XENLA 326 41 -
FSQEIGLASLGAPDDVIEKL PH4H_CAEEL 302 41 -
FSQEIGLASLGASEEDLKKL Q23438 412 41 -
MSQDIGLMSLGASDEHIEKL TY3H_CAEEL 249 41 -

Motif 4 width=19
Element Seqn Id St Int Rpt
AYGAGLLSSYGELLHSLSE TY3H_RAT 388 20 -
AYGAGLLSSYGELLHSLSE TY3H_MOUSE 388 20 -
AYGAGLLSSYGELLHCLSE TY3H_HUMAN 418 20 -
AYGAGLLSSFGELQYCLSD PH4H_RAT 342 20 -
AYGAGLLSSFGELQYCLSE PH4H_HUMAN 342 20 -
AYGAGLLSSYGELLHSLSE TY3H_BOVIN 381 20 -
AYGAGLLSSFGELQYCLSD PH4H_MOUSE 342 20 -
AYGAGLLSSYGELIHSLSD TY3H_PHASP 381 20 -
VYGAGLLSSISELKHSLSG TR5H_CHICK 330 20 -
AYGAGLLSSYGELEYCLTD PH4H_DROME 341 20 -
AYGAGLLSSYGELEYCLTD Q27599 341 20 -
AYGAGLLSSYGELEYCLTD Q27600 336 20 -
AYGAGLLSSYGELLHAISD Q24000 466 20 -
AYGAGLLSSYGELLHAISD TY3H_DROME 395 20 -
AYGAGLLSSYGELVHSLSD TY3H_ANGAN 378 20 -
VFGAGLLSSISELRHALSG TR5H_RAT 329 20 -
VFGAGLLSSISELKHALSG TR5H_HUMAN 329 20 -
VFGAGLLSSISELKHALSG TR5H_MOUSE 332 20 -
VFGAGLLSSISELKHVLSG TR5H_RABIT 329 20 -
VYGAGLLSSISELKHSLSG TR5H_XENLA 366 20 -
AYGAGLLSSFGELQYALSD PH4H_CAEEL 342 20 -
VYGAGLLSSAGELQHAVEG Q23438 465 33 -
AIGAGLLSAYGELMHACSD TY3H_CAEEL 289 20 -

Motif 5 width=20
Element Seqn Id St Int Rpt
AVQPYQDQTYQPVYFVSESF TY3H_RAT 419 12 -
AVQPYQDQTYQPVYFVSESF TY3H_MOUSE 419 12 -
AVQPYQDQTYQSVYFVSESF TY3H_HUMAN 449 12 -
ACQEYSVTEFQPLYYVAESF PH4H_RAT 373 12 -
AIQNYTVTEFQPLYYVAESF PH4H_HUMAN 373 12 -
AVQPYQDQTYQPVYFVSESF TY3H_BOVIN 412 12 -
ACQEYTVTEFRPLYYVAESF PH4H_MOUSE 373 12 -
AVQPCQDQPYQPVYFVSESF TY3H_PHASP 412 12 -
CKQECLITTFQEVYFVSESF TR5H_CHICK 361 12 -
GVTKYPITQFQPLYYVADSF PH4H_DROME 372 12 -
GVTKYPITQFQPLYYVADSF Q27599 372 12 -
GVTKYPITQFQPLYYVADSF Q27600 367 12 -
AVQPYQDQEYQPIYYVAESF Q24000 497 12 -
AVQPYQDQEYQPIYYVAESF TY3H_DROME 426 12 -
AAEPYQDQNYQSVYFVSESF TY3H_ANGAN 409 12 -
CKQECLITSFQDVYFVSESF TR5H_RAT 360 12 -
CKQECLITTFQDVYFVSESF TR5H_HUMAN 360 12 -
CKQECLITSFQDVYFVSESF TR5H_MOUSE 363 12 -
YKQECLITTFQDVYFVSESF TR5H_RABIT 360 12 -
CNQECIITSFQELYFVSESF TR5H_XENLA 397 12 -
CVTKYPITEYQPKYFLAESF PH4H_CAEEL 373 12 -
VEQECLITTFQSAYFYTRNF Q23438 496 12 -
AVQKYEDDDYQPLYFVADSI TY3H_CAEEL 320 12 -

Motif 6 width=19
Element Seqn Id St Int Rpt
RPFSVKFDPYTLAIDVLDS TY3H_RAT 454 15 -
RPFSVKFDPYTLAIDVLDS TY3H_MOUSE 454 15 -
RPFSVKFDPYTLAIDVLDS TY3H_HUMAN 484 15 -
RPFSVRYDPYTQRVEVLDN PH4H_RAT 408 15 -
RPFSVRYDPYTQRIEVLDN PH4H_HUMAN 408 15 -
RPFSVKFDPYTLAIDVLDS TY3H_BOVIN 447 15 -
RPFSVRYDPYTQRVEVLDN PH4H_MOUSE 408 15 -
RPFSVKYEPYTHSIELLDS TY3H_PHASP 447 15 -
RPFGVKYNPYTQSVQILKD TR5H_CHICK 396 15 -
RPFGVRYNAYTQSVEVLDS PH4H_DROME 407 15 -
RPFGVRYNAYTQSVEVLDS Q27599 407 15 -
RPFGVRYNAYTQSVEVLDS Q27600 402 15 -
RPFEVRFNPHTERVEVLDS Q24000 532 15 -
RPFEVRFNPHTERVEVLDS TY3H_DROME 461 15 -
RPFSVRFDPYTYSIEVLDN TY3H_ANGAN 444 15 -
RPFGVKYNPYTQSIQVLRD TR5H_RAT 395 15 -
RPFGVKYNPYTRSIQILKD TR5H_HUMAN 395 15 -
RPFGLKYNPYTQSVQVLRD TR5H_MOUSE 398 15 -
RPFGVKYNPYTRSIQILKD TR5H_RABIT 395 15 -
RPFGLKYNPFTQSVDILKD TR5H_XENLA 432 15 -
RPFQIRYNAYTQRVEILDK PH4H_CAEEL 417 24 -
RPFIVRYNPYTESVEVLNN Q23438 531 15 -
RPFSVVYDPFTKSIEAIES TY3H_CAEEL 355 15 -