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PR00033

Identifier
HTHASNC  [View Relations]  [View Alignment]  
Accession
PR00033
No. of Motifs
3
Creation Date
17-SEP-1993  (UPDATE 17-JUN-1999)
Title
AsnC bacterial regulatory protein HTH signature
Database References

PROSITE; PS00519 HTH_ASNC_FAMILY
BLOCKS; BL00519
PFAM; PF01037 ASNC_trans_reg
INTERPRO; IPR000485
Literature References
1. WILLINS, D.A., RYAN, C., PLATKO, J.V. AND CALVO, J.M.
Characterisation of LRP, an Escherichia coli regulatory protein that
mediates a global response to leucine.
J.BIOL.CHEM. 266(17) 10768-10774 (1991).
 
2. BOLOTIN, A. AND BIRO, S.
Nucleotide sequence of the putative regulatory gene and major promoter
region of the Streptomyces griseus glycerol operon.
GENE 87(1) 151-152 (1990).

Documentation
Numerous bacterial transcription regulatory proteins bind DNA via a 
helix-turn-helix (HTH) motif. These proteins are very diverse, but for
convenience may be grouped into subfamilies on the basis of sequence
similarity. One such family includes the E.coli asnC and leucine-responsive
regulatory proteins [1], and the Streptomyces glycerol operon regulatory
protein [2]. The HTH motif in each of these proteins is situated towards
the N-terminus.
 
HTHASNC is a 3-element fingerprint that provides a signature for the HTH
motif of the asnC bacterial regulatory proteins. The fingerprint was
derived from an initial alignment of 4 sequences: the motifs completely
encompass the HTH motif and extend slightly beyond it in both N- and C-
terminal directions, motifs 2 and 3 spanning the region encoded by PROSITE
pattern HTH_ASNC_FAMILY (PS00519). A single iteration on OWL21.1 was
required to reach convergence, no new sequences being identified beyond
the starting set. 
 
An update on SPTR37_9f identified a true set of 36 sequences, and 13
partial matches.
Summary Information
  36 codes involving  3 elements
12 codes involving 2 elements
Composite Feature Index
3363636
212012
123
True Positives
ASNC_ECOLI    AZLB_BACSU    BKDR_PSEPU    LRP_ECOLI     
LRP_HAEIN LRP_KLEPN LRP_SALTY LRP_SERMA
O05140 O05217 O29117 O29671
O31497 O33321 O33467 O54287
O57802 O57818 O57880 O58752
O58782 O59256 O59309 O87635
P94329 P95905 P96582 P96896
Q44333 Q52710 Q53106 Y151_METJA
Y224_HAEIN Y4TD_RHISN Y723_METJA YGDH_PYRFU
True Positive Partials
Codes involving 2 elements
ASNC_HAEIN GRP_ZYMMO O27261 O28531
O29776 O58741 O59188 O59579
O86743 P71888 YBAO_ECOLI ZRP_ZYMMO
Sequence Titles
ASNC_ECOLI  REGULATORY PROTEIN ASNC - ESCHERICHIA COLI.   
AZLB_BACSU TRANSCRIPTIONAL REGULATOR AZLB - BACILLUS SUBTILIS.
BKDR_PSEPU BKD OPERON TRANSCRIPTIONAL REGULATOR - PSEUDOMONAS PUTIDA.
LRP_ECOLI LEUCINE-RESPONSIVE REGULATORY PROTEIN - ESCHERICHIA COLI, AND ENTEROBACTER AEROGENES (AEROBACTER AEROGENES).
LRP_HAEIN LEUCINE-RESPONSIVE REGULATORY PROTEIN - HAEMOPHILUS INFLUENZAE.
LRP_KLEPN LEUCINE-RESPONSIVE REGULATORY PROTEIN - KLEBSIELLA PNEUMONIAE.
LRP_SALTY LEUCINE-RESPONSIVE REGULATORY PROTEIN - SALMONELLA TYPHIMURIUM.
LRP_SERMA LEUCINE-RESPONSIVE REGULATORY PROTEIN - SERRATIA MARCESCENS.
O05140 LRP AND FTSK GENES - PROTEUS MIRABILIS.
O05217 SIMILAR TO LEUCINE REGULATORY PROTEIN - BACILLUS SUBTILIS.
O29117 TRANSCRIPTIONAL REGULATORY PROTEIN, ASNC FAMILY - ARCHAEOGLOBUS FULGIDUS.
O29671 TRANSCRIPTIONAL REGULATORY PROTEIN, ASNC FAMILY - ARCHAEOGLOBUS FULGIDUS.
O31497 YEZC PROTEIN - BACILLUS SUBTILIS.
O33321 TRANSCRIPTIONAL REGULATOR - MYCOBACTERIUM TUBERCULOSIS.
O33467 LRP-FAMILY TRANSCRIPTIONAL REGULATORS - PSEUDOMONAS PUTIDA.
O54287 LEUCINE-RESPONSIVE-REGULATORY PROTEIN - SULFOLOBUS SOLFATARICUS.
O57802 151AA LONG HYPOTHETICAL PROTEIN - PYROCOCCUS HORIKOSHII.
O57818 155AA LONG HYPOTHETICAL PROTEIN - PYROCOCCUS HORIKOSHII.
O57880 158AA LONG HYPOTHETICAL TRANSCRIPTIONAL REGULATOR - PYROCOCCUS HORIKOSHII.
O58752 193AA LONG HYPOTHETICAL PROTEIN - PYROCOCCUS HORIKOSHII.
O58782 162AA LONG HYPOTHETICAL PROTEIN - PYROCOCCUS HORIKOSHII.
O59256 141AA LONG HYPOTHETICAL TRANSCRIPTIONAL REGULATOR - PYROCOCCUS HORIKOSHII.
O59309 151AA LONG HYPOTHETICAL TRANSCRIPTIONAL REGULATOR - PYROCOCCUS HORIKOSHII.
O87635 LEUCINE-RESPONSIVE REGULATORY PROTEIN - KLEBSIELLA AEROGENES.
P94329 LEUCINE-RESPONSIVE REGULATORY PROTEIN - BRADYRHIZOBIUM JAPONICUM.
P95905 ORF C01007 - SULFOLOBUS SOLFATARICUS.
P96582 YDAI PROTEIN (TRANSCRIPTIONAL REGULATOR (LRP/ASNC FAMILY)) - BACILLUS SUBTILIS.
P96896 HYPOTHETICAL 16.5 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
Q44333 PLASMID PATR10 PROLINE DEHYDROGENASE (PUTA) AND PRP (PRP) GENES, COMPLETE CDS (PUTA) (PRP) - AGROBACTERIUM TUMEFACIENS.
Q52710 (B10S) - RHODOBACTER CAPSULATUS (RHODOPSEUDOMONAS CAPSULATA).
Q53106 PUTATIVE ASNC-LRP FAMILY REGULATORY PROTEIN - RHODOCOCCUS SP.
Y151_METJA HYPOTHETICAL TRANSCRIPTIONAL REGULATOR PROTEIN MJ0151 - METHANOCOCCUS JANNASCHII.
Y224_HAEIN HYPOTHETICAL TRANSCRIPTIONAL REGULATOR HI0224 - HAEMOPHILUS INFLUENZAE.
Y4TD_RHISN HYPOTHETICAL TRANSCRIPTIONAL REGULATOR Y4TD - RHIZOBIUM SP. (STRAIN NGR234).
Y723_METJA HYPOTHETICAL TRANSCRIPTIONAL REGULATOR PROTEIN MJ0723 - METHANOCOCCUS JANNASCHII.
YGDH_PYRFU HYPOTHETICAL TRANSCRIPTIONAL REGULATOR IN GDH 3'REGION - PYROCOCCUS FURIOSUS.

ASNC_HAEIN REGULATORY PROTEIN ASNC - HAEMOPHILUS INFLUENZAE.
GRP_ZYMMO GLUTAMATE UPTAKE REGULATORY PROTEIN - ZYMOMONAS MOBILIS.
O27261 TRANSCRIPTIONAL REGULATOR - METHANOBACTERIUM THERMOAUTOTROPHICUM.
O28531 TRANSCRIPTIONAL REGULATORY PROTEIN, ASNC FAMILY - ARCHAEOGLOBUS FULGIDUS.
O29776 TRANSCRIPTIONAL REGULATORY PROTEIN, ASNC FAMILY - ARCHAEOGLOBUS FULGIDUS.
O58741 162AA LONG HYPOTHETICAL PROTEIN - PYROCOCCUS HORIKOSHII.
O59188 151AA LONG HYPOTHETICAL TRANSCRIPTIONAL REGULATOR - PYROCOCCUS HORIKOSHII.
O59579 150AA LONG HYPOTHETICAL LEUCINE-RESPONSIVE REGULATORY PROTEIN - PYROCOCCUS HORIKOSHII.
O86743 TRANSCRIPTIONAL REGULATOTY PROTEIN - STREPTOMYCES COELICOLOR.
P71888 HYPOTHETICAL 16.4 KD PROTEIN CY3G12.10 - MYCOBACTERIUM TUBERCULOSIS.
YBAO_ECOLI HYPOTHETICAL TRANSCRIPTIONAL REGULATOR IN MDL-COF INTERGENIC REGION - ESCHERICHIA COLI.
ZRP_ZYMMO GLOBAL REGULATORY PROTEIN - ZYMOMONAS MOBILIS.
Scan History
OWL21_1    1  100  NSINGLE    
OWL25_2 2 100 NSINGLE
SPTR37_9f 6 85 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
LERAAAMLRLLAGGERR P15360 7 7 -
LERAAAMLRLLAGGERR P22866 7 7 -
LDRIDRNILNELQKDGR LRP_ECOLI 11 11 -
IDNLDRGILEALMGNAR ASNC_ECOLI 5 5 -

Motif 2 width=12
Element Seqn Id St Int Rpt
RLGLSDIASTLG P22866 23 -1 -
RISNVELSKRVG LRP_ECOLI 27 -1 -
RTAYAELAKQFG ASNC_ECOLI 21 -1 -
RLGLSDIASSLG P15360 23 -1 -

Motif 3 width=20
Element Seqn Id St Int Rpt
GLAKGTAHGILRTLQQEGFV P15360 34 -1 -
GLAKGTAHGILRSLQAEGFV P22866 34 -1 -
GLSPTPCLERVRRLERQGFI LRP_ECOLI 38 -1 -
GVSPGTIHVRVEKMKQAGII ASNC_ECOLI 32 -1 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
LDRIDRNILNELQKDGR O87635 12 12 -
LDRIDRNILNELQKDGR O05140 12 12 -
LDRIDRNILNELQKDGR LRP_SERMA 11 11 -
LDRIDRNILNELQKDGR LRP_SALTY 11 11 -
LDRIDRNILNELQKDGR LRP_KLEPN 11 11 -
LDRIDRNILNELQKDGR LRP_ECOLI 11 11 -
LDRLDRRILSILQEDGR P94329 3 3 -
LDAIDIKILNELQRNGK LRP_HAEIN 16 16 -
LDRTDIGILNSLQENAR BKDR_PSEPU 4 4 -
LDHFDLKILEALSEDGR Q44333 10 10 -
IDERDKIILEILSKDAR O59256 2 2 -
IDERDKIILEILEKDAR YGDH_PYRFU 2 2 -
IDATDRRILHELCANAR Q52710 5 5 -
LDRIDLKILRILNGNAR Y151_METJA 2 2 -
MDDTDLQILSHLQRNGR O31497 1 1 -
IDEIDEVIVRELRKNSR O57818 4 4 -
LDRADVALLNAVQKNNR Y4TD_RHISN 18 18 -
LDEIDRAILRLLQEDGR O57880 12 12 -
LDDIDRILVRELAADGR P96896 5 5 -
LDEVDRRILSLLHGDAR O33321 24 24 -
LDETDKAILRDLQEDAS AZLB_BACSU 5 5 -
LDQIDLNIIEELKKDSR P96582 90 90 -
LDALDRKILEILLKDSR O59309 6 6 -
MDEKDLKIIEILMRDGR Y723_METJA 17 17 -
IDEVDIKILRELQDDAR O29117 4 4 -
MDEKDMLILSELVKDSR O29671 1 1 -
LDETDKQILTILHEEGR O05217 12 12 -
IDNLDRGILEALMGNAR ASNC_ECOLI 5 5 -
IDRTDRALLAALQDNAR O33467 5 5 -
LDKLDRHILNVLQQDAM Y224_HAEIN 19 19 -
LDDTDEKILNILRYNAK P95905 9 9 -
IDKLDVQLLGLLSKDSR Q53106 4 4 -
LDDLDIMIYKMLREDGR O58752 41 41 -
IDAIDKKLLIELLKDSR O54287 9 9 -
LSKKDWEIIKLLKKDAR O58782 9 9 -
LDRVDMQLVKILSENSR O57802 7 7 -

Motif 2 width=12
Element Seqn Id St Int Rpt
RISNVELSKRVG O87635 28 -1 -
RISNVELSKRVG O05140 28 -1 -
RISNVELSKRVG LRP_SERMA 27 -1 -
RISNVELSKRVG LRP_SALTY 27 -1 -
RISNVELSKRVG LRP_KLEPN 27 -1 -
RISNVELSKRVG LRP_ECOLI 27 -1 -
RIANVELAERIG P94329 19 -1 -
KISNIDLSKKVG LRP_HAEIN 32 -1 -
RITNAELARSVN BKDR_PSEPU 20 -1 -
RMSVLQLSKRVG Q44333 26 -1 -
RTPFTEIAKKLG O59256 18 -1 -
RTPFTEIAKKLG YGDH_PYRFU 18 -1 -
RIPVTELARKVG Q52710 21 -1 -
RKSFREIGRELG Y151_METJA 18 -1 -
RLTMVELGKLVG O31497 17 -1 -
RITLTELGRKVG O57818 20 -1 -
RLTSEELADKVG Y4TD_RHISN 34 -1 -
RMSYSEISRRIN O57880 28 -1 -
RATLSELATRAG P96896 21 -1 -
RMPNNALADTVG O33321 40 -1 -
SISNLNLSKKIG AZLB_BACSU 21 -1 -
RLSMRELGRKIK P96582 106 -1 -
RTSYREIAKDLN O59309 22 -1 -
RKSYTDIARELG Y723_METJA 33 -1 -
RKSLKEISEKVG O29117 20 -1 -
RKTLSELAEMLD O29671 17 -1 -
RISYTDLGKRVD O05217 28 -1 -
RTAYAELAKQFG ASNC_ECOLI 21 -1 -
RLTVAELADSVA O33467 21 -1 -
MIPLKELSEKVN Y224_HAEIN 35 -1 -
KKSLKELSDELG P95905 25 -1 -
RMSVAELANSLG Q53106 20 -1 -
RISDSRIAERLG O58752 57 -1 -
RISLRRLAEEMN O54287 25 -1 -
RMSDAEIGRRIG O58782 25 -1 -
RLTYRELADILN O57802 23 -1 -

Motif 3 width=20
Element Seqn Id St Int Rpt
GLSPTPCLERVRRLERQGFI O87635 39 -1 -
GLSPTPCLERVRRLERQGFI O05140 39 -1 -
GLSPTPCLERVRRLERQGFI LRP_SERMA 38 -1 -
GLSPTPCLERVRRLERQGFI LRP_SALTY 38 -1 -
GLSPTPCLERVRRLERQGFI LRP_KLEPN 38 -1 -
GLSPTPCLERVRRLERQGFI LRP_ECOLI 38 -1 -
GLSPTSIGERLKRLQREGFV P94329 30 -1 -
GLSPTPCLERVKRLEKQGVI LRP_HAEIN 43 -1 -
NLSPTPCFNRVRAMEELGVI BKDR_PSEPU 31 -1 -
GLSKTPCQTRLKRLVDEGYI Q44333 37 -1 -
GISETAVRKRVKALEEKGII O59256 29 -1 -
GISETAVRKRVKALEEKGII YGDH_PYRFU 29 -1 -
GLSKTPVAARIRAMEEMGLI Q52710 32 -1 -
GISEGTVRNRVKRLTEKGII Y151_METJA 29 -1 -
GLSSPSAAERVRKLEDKGVI O31497 28 -1 -
GLTASAVKNRIEKLEKLGVI O57818 31 -1 -
GLSPTACQRRLKRLRSLGVI Y4TD_RHISN 45 -1 -
NVPESTVRARVNRLVKEGVI O57880 39 -1 -
GLSVSAVQSRVRRLESRGVV P96896 32 -1 -
GIAPSTCHGRVRRLVDLGVI O33321 51 -1 -
GLSPSACLARTKNLVEAGII AZLB_BACSU 32 -1 -
KLSPPSVTERVRQLESFGII P96582 117 -1 -
NVAVGTIYNRIKKLEDSGVI O59309 33 -1 -
GTSESSIRKRVKKLEEEGVI Y723_METJA 44 -1 -
GVAEGTVYNRINKMRKIGLI O29117 31 -1 -
DMSVSSIHKRVKKLEKEGVI O29671 28 -1 -
DLSRVAVQARINQLIEAGVI O05217 39 -1 -
GVSPGTIHVRVEKMKQAGII ASNC_ECOLI 32 -1 -
ALTTSPCWRRVKLLEESGYI O33467 32 -1 -
NSSVATCQRRVQSLTDSGII Y224_HAEIN 46 -1 -
GIPISTVRYRIKRLEDAQII P95905 36 -1 -
GVARNTVQSRMKRMESNGLL Q53106 31 -1 -
GVSVTTVRRHRLKLQREGIL O58752 68 -1 -
NVSPATLHNRLMRLVQEGVV O54287 36 -1 -
GLSKSAVRWRRINLQKRGYL O58782 36 -1 -
NTTRQRIARRIDKLKKLGII O57802 34 -1 -