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PR01989

Identifier
EUOM20RECPTR  [View Relations]  [View Alignment]  
Accession
PR01989
No. of Motifs
7
Creation Date
16-APR-2009
Title
Eukaryotic MAS20 (TOM20) import receptor subunit signature
Database References
PRINTS; PR00351 OM20RECEPTOR
PFAM; PF02064 MAS20
INTERPRO; IPR002056
PDB; 1OM2; 2V1S; 2V1T
SCOP; 1OM2; 2V1S; 2V1T
CATH; 1OM2; 2V1S; 2V1T
MIM; 601848
Literature References
1. LITHGOW, T., GLICK, B.S. AND GOTTFRIED, S.
The protein import receptor of mitochondria.
TRENDS BIOCHEM.SCI. 20 98-101 (1995).
 
2. MILLAR, D.G. AND SHORE, G.C.
Mitochondrial MAS70P signal anchor sequence - mutations in the 
transmembrane domain that disrupt dimerisation but not targeting
or membrane insertion.
J.BIOL.CHEM. 269 12229-12232 (1994).
 
3. GOPING, I.S., MILLAR, D.G. AND SHORE, G.C.
Identification of the human mitochondrial protein import receptor, huMas20p.
Complementation of delta mas20 in yeast.
FEBS Lett. 373 45-50 (1995).
 
4. ABE, Y., SHODAI, T., MUTO, T., MIHARA, K., TORII, H., NISHIKAWA, S.,
ENDO, T. AND KOHDA, D. 
Structural basis of presequence recognition by the mitochondrial protein
import receptor Tom20. 
CELL 100 551-560 (2000). 

Documentation
Virtually all mitochondrial precursors are imported via the same 
mechanism [1]: precursors first bind to receptors on the mitochondrial
surface, then insert into the translocation channel in the outer membrane.
Many outer-membrane proteins participate in the early stages of import,
4 of which (MAS20, MAS22, MAS37 and MAS70) are components of the receptor.
MAS20, which forms a subcomplex with MAS22, seems to interact with most or
all mitochondrial precursors, suggesting that the protein binds directly
to mitochondrial targeting sequences. The MAS37 and MAS70 components also
form a subcomplex, the 2 subcomplexes possibly binding via their trans-
membrane (TM) regions - the TM region of MAS70 promotes oligomerisation
of attatched protein domains and shares sequence similarity with the
TM region of MAS20 [2]. 
 
The human homologue of the fungal MAS20 (TOM20) import receptor has been
identified and characterised [3]. Although the amino acid sequences exhibit
pronounced differences, similarity is greatest in the N-terminal third of
the molecules. The structure of the rat TOM20 import receptor has been
determined by NMR [4]. The cytosolic domain forms an all-alpha structure,
with a hydrophobic groove that mediates presequence binding [4].
 
EUOM20RECPTR is a 7-element fingerprint that provides a signature for the
metazoan eukaryotic MAS20 (TOM20) import receptor subunit. The fingerprint 
was derived from an initial alignment of 11 sequences: the motifs were drawn
from conserved regions spanning the full alignment length, focusing on those
sections that characterise the eukaryotic receptors but distinguish them
from the rest of the MAS20 (TOM20) family. Two iterations on SPTR55_38f 
were required to reach convergence, at which point a true set comprising 12
sequences was identified. Seven partial matches were also found, all of
which are TOM20-like proteins that fail to make significant matches with
two or more motifs.
Summary Information
  12 codes involving  7 elements
0 codes involving 6 elements
2 codes involving 5 elements
1 codes involving 4 elements
2 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
712121212121212
60000000
52211220
41101010
31101120
20000211
1234567
True Positives
A7RJB2_NEMVE  A8NN61_BRUMA  Q4KL41_MOUSE  Q6PC05_DANRE  
TOM20_BOVIN TOM20_DANRE TOM20_HUMAN TOM20_MOUSE
TOM20_PONPY TOM20_RAT TOM20_XENLA TOM20_XENTR
True Positive Partials
Codes involving 5 elements
Q172F6_AEDAE Q95RF6_DROME
Codes involving 4 elements
OM20_CAEEL
Codes involving 3 elements
A8Y3V5_CAEBR Q9VGX9_DROME
Codes involving 2 elements
Q4V5L5_DROME TO20L_MOUSE
Sequence Titles
A7RJB2_NEMVE Predicted protein - Nematostella vectensis (Starlet sea anemone). 
A8NN61_BRUMA MAS20 protein import receptor containing protein - Brugia malayi (Filarial nematode worm).
Q4KL41_MOUSE Translocase of outer mitochondrial membrane 20 homolog (Yeast) (10 day old male pancreas cDNA, RIKEN full-length enriched library, clone:1810060K07 product:translocase of outer mitochondrial membrane 20 homolog (yeast), full insert sequence) (7 days embryo whole body cDNA, RIKEN full-length enriched library, clone:C430040O05 product:translocase of outer mitochondrial membrane 20 homolog (yeast), full insert sequence) - Mus musculus (Mouse).
Q6PC05_DANRE Translocase of outer mitochondrial membrane 20 homolog (Yeast) - Danio rerio (Zebrafish) (Brachydanio rerio).
TOM20_BOVIN Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial 20 kDa outer membrane protein) (Outer mitochondrial membrane receptor Tom20) - Bos taurus (Bovine).
TOM20_DANRE Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial 20 kDa outer membrane protein) (Outer mitochondrial membrane receptor Tom20) - Danio rerio (Zebrafish) (Brachydanio rerio).
TOM20_HUMAN Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial 20 kDa outer membrane protein) (Outer mitochondrial membrane receptor Tom20) - Homo sapiens (Human).
TOM20_MOUSE Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial 20 kDa outer membrane protein) (Outer mitochondrial membrane receptor Tom20) - Mus musculus (Mouse).
TOM20_PONPY Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial 20 kDa outer membrane protein) (Outer mitochondrial membrane receptor Tom20) - Pongo pygmaeus (Orangutan).
TOM20_RAT Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial 20 kDa outer membrane protein) (Outer mitochondrial membrane receptor Tom20) - Rattus norvegicus (Rat).
TOM20_XENLA Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial 20 kDa outer membrane protein) (Outer mitochondrial membrane receptor Tom20) - Xenopus laevis (African clawed frog).
TOM20_XENTR Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial 20 kDa outer membrane protein) (Outer mitochondrial membrane receptor Tom20) - Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).

Q172F6_AEDAE Mitochondrial import receptor subunit tom20 - Aedes aegypti (Yellowfever mosquito).
Q95RF6_DROME LD34461p (CG7654-PA) - Drosophila melanogaster (Fruit fly).

OM20_CAEEL MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20 HOMOLOG (MITOCHONDRIAL 20 KD OUTER MEMBRANE PROTEIN) (OUTER MITOCHONDRIAL MEMBRANE RECEPTOR TOM20) - CAENORHABDITIS ELEGANS.

A8Y3V5_CAEBR Putative uncharacterized protein - Caenorhabditis briggsae.
Q9VGX9_DROME CG14690-PA - Drosophila melanogaster (Fruit fly).

Q4V5L5_DROME IP07008p (IP06908p) - Drosophila melanogaster (Fruit fly).
TO20L_MOUSE TOMM20-like protein 1 - Mus musculus (Mouse).
Scan History
SPTR55_38f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
GRNSAIAAGVCGALFIGY Q4KL41_MOUSE 3 3 -
GRNSAIAAGVCGALFIGY TOM20_RAT 3 3 -
GRNSAIAAGVCGALFIGY TOM20_MOUSE 3 3 -
GRNSAIAAGVCGALFIGY TOM20_BOVIN 3 3 -
GRNSAIAAGVCGALFIGY TOM20_HUMAN 3 3 -
GKTSAIAAGVCGALFLGY TOM20_XENTR 5 5 -
GSSSRIAAGLGAALFVGY TOM20_DANRE 4 4 -
GKTSAIAAGVCGALLLGY TOM20_XENLA 5 5 -
GRPGAVALGVCGALFIGY Q6PC05_DANRE 3 3 -
SQIATVVAGVCGSMFLAY A7RJB2_NEMVE 4 4 -
RQRIVTAAGIAAAAFVGY A8NN61_BRUMA 13 13 -

Motif 2 width=17
Element Seqn Id St Int Rpt
PNFKNRLRERRKKQKLA Q4KL41_MOUSE 32 11 -
PNFKNRLRERRKKQKLA TOM20_RAT 32 11 -
PNFKNRLRERRKKQKLA TOM20_MOUSE 32 11 -
PNFKNRLRERRKKQKLA TOM20_BOVIN 32 11 -
PNFKNRLRERRKKQKLA TOM20_HUMAN 32 11 -
PNFKNRLREKRRKQKIA TOM20_XENTR 34 11 -
PNYKNKLRERRKKQKAA TOM20_DANRE 33 11 -
PNFKNRLREKRRKQKIA TOM20_XENLA 34 11 -
PNFRTKLRERRKKQRTA Q6PC05_DANRE 32 11 -
PDYKKKLIEKRAQRQEA A7RJB2_NEMVE 33 11 -
PDYKQKIREQRRATEKK A8NN61_BRUMA 42 11 -

Motif 3 width=14
Element Seqn Id St Int Rpt
PDLKDAEAVQKFFL Q4KL41_MOUSE 58 9 -
PDLKDAEAVQKFFL TOM20_RAT 58 9 -
PDLKDAEAVQKFFL TOM20_MOUSE 58 9 -
PDLKDAEAVQKFFL TOM20_BOVIN 58 9 -
PDLKDAEAVQKFFL TOM20_HUMAN 58 9 -
PDLKDAEAVQKFFL TOM20_XENTR 60 9 -
PDLKDAEAVQKFFL TOM20_DANRE 59 9 -
PDLKDAEAVQKFFL TOM20_XENLA 60 9 -
PDLKDAAAVQKFFL Q6PC05_DANRE 58 9 -
PDLTDTAAVQKFFL A7RJB2_NEMVE 59 9 -
PNPHNATEMQAFFL A8NN61_BRUMA 65 6 -

Motif 4 width=15
Element Seqn Id St Int Rpt
EIQLGEELLAQGDYE Q4KL41_MOUSE 73 1 -
EIQLGEELLAQGDYE TOM20_RAT 73 1 -
EIQLGEELLAQGDYE TOM20_MOUSE 73 1 -
EIQLGEELLAQGEYE TOM20_BOVIN 73 1 -
EIQLGEELLAQGEYE TOM20_HUMAN 73 1 -
EIQLGEELLAQGDFE TOM20_XENTR 75 1 -
EIQLGEELLAQGDYE TOM20_DANRE 74 1 -
EIQLGEELLAQGDFE TOM20_XENLA 75 1 -
EIQLGEELLAQGDYE Q6PC05_DANRE 73 1 -
EVQIGEDLLTKGEYE A7RJB2_NEMVE 74 1 -
EVQLGEELVADGHTE A8NN61_BRUMA 80 1 -

Motif 5 width=10
Element Seqn Id St Int Rpt
HLTNAIAVCG Q4KL41_MOUSE 92 4 -
HLTNAIAVCG TOM20_RAT 92 4 -
HLTNAIAVCG TOM20_MOUSE 92 4 -
HLTNAIAVCG TOM20_BOVIN 92 4 -
HLTNAIAVCG TOM20_HUMAN 92 4 -
HLTNAIAICG TOM20_XENTR 94 4 -
HLTNAIAVCG TOM20_DANRE 93 4 -
HLTNAIAICG TOM20_XENLA 94 4 -
HLTNAIAVCG Q6PC05_DANRE 92 4 -
HLTNAVAVCG A7RJB2_NEMVE 93 4 -
HLCNAITLCG A8NN61_BRUMA 99 4 -

Motif 6 width=13
Element Seqn Id St Int Rpt
PQQLLQVLQQTLP Q4KL41_MOUSE 103 1 -
PQQLLQVLQQTLP TOM20_RAT 103 1 -
PQQLLQVLQQTLP TOM20_MOUSE 103 1 -
PQQLLQVLQQTLP TOM20_BOVIN 103 1 -
PQQLLQVLQQTLP TOM20_HUMAN 103 1 -
PQQLLQVLQQTLP TOM20_XENTR 105 1 -
PQQLLQVLQQTLP TOM20_DANRE 104 1 -
PQQLLQVLQQTLP TOM20_XENLA 105 1 -
PQQLLQVLQQTLP Q6PC05_DANRE 103 1 -
PQQLLQVFKQTLP A7RJB2_NEMVE 104 1 -
PAQLIQIFQQTLP A8NN61_BRUMA 110 1 -

Motif 7 width=18
Element Seqn Id St Int Rpt
FQMLLTKLPTISQRIVSA Q4KL41_MOUSE 119 3 -
FQMLLTKLPTISQRIVSA TOM20_RAT 119 3 -
FQMLLTKLPTISQRIVSA TOM20_MOUSE 119 3 -
FQMLLTKLPTISQRIVSA TOM20_BOVIN 119 3 -
FQMLLTKLPTISQRIVSA TOM20_HUMAN 119 3 -
FQMLLTKLPTINQRIGNA TOM20_XENTR 121 3 -
FQMLLTKLPTISQRIVSA TOM20_DANRE 120 3 -
FQMLLTKLPTISQRIGNA TOM20_XENLA 121 3 -
FQMLLTKLPTISQRMVSA Q6PC05_DANRE 119 3 -
FQMLIDNINQMKDVTHKV A7RJB2_NEMVE 120 3 -
FDLLIQKLPETKTRLMRM A8NN61_BRUMA 126 3 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
GRNSAIAAGVCGALFIGY Q4KL41_MOUSE 3 3 -
GRNSAIAAGVCGALFIGY TOM20_RAT 3 3 -
GRNSAIAAGVCGALFIGY TOM20_MOUSE 3 3 -
GRNSAIAAGVCGALFIGY TOM20_BOVIN 3 3 -
GRNSAIAAGVCGALFIGY TOM20_PONPY 3 3 -
GRNSAIAAGVCGALFIGY TOM20_HUMAN 3 3 -
GKTSAIAAGVCGALFLGY TOM20_XENTR 5 5 -
GSSSRIAAGLGAALFVGY TOM20_DANRE 4 4 -
GKTSAIAAGVCGALLLGY TOM20_XENLA 5 5 -
GRPGAVALGVCGALFIGY Q6PC05_DANRE 3 3 -
SQIATVVAGVCGSMFLAY A7RJB2_NEMVE 4 4 -
RQRIVTAAGIAAAAFVGY A8NN61_BRUMA 13 13 -

Motif 2 width=17
Element Seqn Id St Int Rpt
PNFKNRLRERRKKQKLA Q4KL41_MOUSE 32 11 -
PNFKNRLRERRKKQKLA TOM20_RAT 32 11 -
PNFKNRLRERRKKQKLA TOM20_MOUSE 32 11 -
PNFKNRLRERRKKQKLA TOM20_BOVIN 32 11 -
PNFKNRLRERRKKQKLA TOM20_PONPY 32 11 -
PNFKNRLRERRKKQKLA TOM20_HUMAN 32 11 -
PNFKNRLREKRRKQKIA TOM20_XENTR 34 11 -
PNYKNKLRERRKKQKAA TOM20_DANRE 33 11 -
PNFKNRLREKRRKQKIA TOM20_XENLA 34 11 -
PNFRTKLRERRKKQRTA Q6PC05_DANRE 32 11 -
PDYKKKLIEKRAQRQEA A7RJB2_NEMVE 33 11 -
PDYKQKIREQRRATEKK A8NN61_BRUMA 42 11 -

Motif 3 width=14
Element Seqn Id St Int Rpt
PDLKDAEAVQKFFL Q4KL41_MOUSE 58 9 -
PDLKDAEAVQKFFL TOM20_RAT 58 9 -
PDLKDAEAVQKFFL TOM20_MOUSE 58 9 -
PDLKDAEAVQKFFL TOM20_BOVIN 58 9 -
PDLKDAEAVQKFFL TOM20_PONPY 58 9 -
PDLKDAEAVQKFFL TOM20_HUMAN 58 9 -
PDLKDAEAVQKFFL TOM20_XENTR 60 9 -
PDLKDAEAVQKFFL TOM20_DANRE 59 9 -
PDLKDAEAVQKFFL TOM20_XENLA 60 9 -
PDLKDAAAVQKFFL Q6PC05_DANRE 58 9 -
PDLTDTAAVQKFFL A7RJB2_NEMVE 59 9 -
PNPHNATEMQAFFL A8NN61_BRUMA 65 6 -

Motif 4 width=15
Element Seqn Id St Int Rpt
EIQLGEELLAQGDYE Q4KL41_MOUSE 73 1 -
EIQLGEELLAQGDYE TOM20_RAT 73 1 -
EIQLGEELLAQGDYE TOM20_MOUSE 73 1 -
EIQLGEELLAQGEYE TOM20_BOVIN 73 1 -
EIQLGEELLAQGEYE TOM20_PONPY 73 1 -
EIQLGEELLAQGEYE TOM20_HUMAN 73 1 -
EIQLGEELLAQGDFE TOM20_XENTR 75 1 -
EIQLGEELLAQGDYE TOM20_DANRE 74 1 -
EIQLGEELLAQGDFE TOM20_XENLA 75 1 -
EIQLGEELLAQGDYE Q6PC05_DANRE 73 1 -
EVQIGEDLLTKGEYE A7RJB2_NEMVE 74 1 -
EVQLGEELVADGHTE A8NN61_BRUMA 80 1 -

Motif 5 width=10
Element Seqn Id St Int Rpt
HLTNAIAVCG Q4KL41_MOUSE 92 4 -
HLTNAIAVCG TOM20_RAT 92 4 -
HLTNAIAVCG TOM20_MOUSE 92 4 -
HLTNAIAVCG TOM20_BOVIN 92 4 -
HLTNAIAVCG TOM20_PONPY 92 4 -
HLTNAIAVCG TOM20_HUMAN 92 4 -
HLTNAIAICG TOM20_XENTR 94 4 -
HLTNAIAVCG TOM20_DANRE 93 4 -
HLTNAIAICG TOM20_XENLA 94 4 -
HLTNAIAVCG Q6PC05_DANRE 92 4 -
HLTNAVAVCG A7RJB2_NEMVE 93 4 -
HLCNAITLCG A8NN61_BRUMA 99 4 -

Motif 6 width=13
Element Seqn Id St Int Rpt
PQQLLQVLQQTLP Q4KL41_MOUSE 103 1 -
PQQLLQVLQQTLP TOM20_RAT 103 1 -
PQQLLQVLQQTLP TOM20_MOUSE 103 1 -
PQQLLQVLQQTLP TOM20_BOVIN 103 1 -
PQQLLQVLQQTLP TOM20_PONPY 103 1 -
PQQLLQVLQQTLP TOM20_HUMAN 103 1 -
PQQLLQVLQQTLP TOM20_XENTR 105 1 -
PQQLLQVLQQTLP TOM20_DANRE 104 1 -
PQQLLQVLQQTLP TOM20_XENLA 105 1 -
PQQLLQVLQQTLP Q6PC05_DANRE 103 1 -
PQQLLQVFKQTLP A7RJB2_NEMVE 104 1 -
PAQLIQIFQQTLP A8NN61_BRUMA 110 1 -

Motif 7 width=18
Element Seqn Id St Int Rpt
FQMLLTKLPTISQRIVSA Q4KL41_MOUSE 119 3 -
FQMLLTKLPTISQRIVSA TOM20_RAT 119 3 -
FQMLLTKLPTISQRIVSA TOM20_MOUSE 119 3 -
FQMLLTKLPTISQRIVSA TOM20_BOVIN 119 3 -
FQMLLTKLPTISQRIVSA TOM20_PONPY 119 3 -
FQMLLTKLPTISQRIVSA TOM20_HUMAN 119 3 -
FQMLLTKLPTINQRIGNA TOM20_XENTR 121 3 -
FQMLLTKLPTISQRIVSA TOM20_DANRE 120 3 -
FQMLLTKLPTISQRIGNA TOM20_XENLA 121 3 -
FQMLLTKLPTISQRMVSA Q6PC05_DANRE 119 3 -
FQMLIDNINQMKDVTHKV A7RJB2_NEMVE 120 3 -
FDLLIQKLPETKTRLMRM A8NN61_BRUMA 126 3 -