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PR01891

Identifier
WNT7PROTEIN  [View Relations]  [View Alignment]  
Accession
PR01891
No. of Motifs
5
Creation Date
12-MAY-2003
Title
Wnt-7 protein signature
Database References
PRINTS; PR01349 WNTPROTEIN
MIM; 601570; 601967
Literature References
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
 
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
 
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.REV. 33 1-12 (2000).
 
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
 
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the 
nucleus.
SCIENCE 287 1606-1609 (2000).
 
6. MOON, R.T.
An introduction to non-canonical Wnt and frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
 
7. GAVIN, B.J., MCMAHON, J.A. AND MCMAHON, A.P.
Expression of multiple novel Wnt-1/int-1-related genes during fetal and 
adult mouse development.
GENES DEV. 4 2319-2332 (1990).I

Documentation
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidney,
regulation of mammary gland proliferation, and Alzheimer's disease [3,4].
 
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of 
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been 
identified in vertebrates, with multiple subtypes within some classes.
 
Wnt-7 cDNA was isolated from mouse using a PCR-based strategy, where it was 
found to be expressed in adult tissues, particularly in brain and lung [7]. 
Two subtypes are known to exist, designated Wnt-7A and B. Wnt-7A has been 
implicated in development of the uterus, cerebellum and limbs; Wnt-7B is
believed to play a role in lung and placental development.
   
WNT7PROTEIN is a 5-element fingerprint that provides a signature for Wnt-7 
proteins. The fingerprint was derived from an initial alignment of 7 
sequences: the motifs were drawn from conserved regions spanning the full 
alignment length, focusing on those sections that characterise Wnt-6 
proteins but distinguish them from other Wnt subtypes - motif 1 lies near 
the N-terminus; motifs 2-4 are situated in the central portion of the 
alignment, between conserved cysteines; and motif 5 resides at the C-
terminus. Two iterations on SPTR40_22f were required to reach convergence, 
at which point a true set comprising 9 sequences was identified.
Summary Information
9 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
599999
400000
300000
200000
12345
True Positives
O13266        O42258        Q96H90        Q9DBY3        
Q9DEB8 WN7A_HUMAN WN7A_MOUSE WN7B_HUMAN
WN7B_MOUSE
Sequence Titles
O13266      Wnt-7a - Pleurodeles waltlii (Iberian ribbed newt). 
O42258 Wnt7B - Xenopus laevis (African clawed frog).
Q96H90 Hypothetical protein - Homo sapiens (Human).
Q9DBY3 Wingless-related MMTV integration site 7A - Mus musculus (Mouse).
Q9DEB8 Wnt-7a - Gallus gallus (Chicken).
WN7A_HUMAN WNT-7A protein precursor - Homo sapiens (Human).
WN7A_MOUSE WNT-7A protein precursor - Mus musculus (Mouse).
WN7B_HUMAN WNT-7B protein precursor - Homo sapiens (Human).
WN7B_MOUSE WNT-7B protein precursor - Mus musculus (Mouse).
Scan History
SPTR40_22f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
GIVYLRIGGFSSVVA WN7A_MOUSE 17 17 -
GIVYIKIGGFSSVVA Q9DEB8 17 17 -
GMVCLRIGGFSSVVA WN7A_HUMAN 17 17 -
GIIYMKLGALSSVVA O42258 17 17 -
GVLYVKLGALSSVVA WN7B_MOUSE 17 17 -
GIVYIKIGGFSSVVA O13266 17 17 -
GVLYVKLGALSSVVA WN7B_HUMAN 17 17 -

Motif 2 width=12
Element Seqn Id St Int Rpt
DKEKQGQYHWDE WN7A_MOUSE 134 102 -
DKEKQGQYHKEE Q9DEB8 134 102 -
DKEKQGQYHRDE WN7A_HUMAN 134 102 -
DREKQGYYNQEE O42258 134 102 -
DREKQGYYNQAE WN7B_MOUSE 134 102 -
DKGKQGQFHIEE O13266 133 101 -
DREKQGYYNQAE WN7B_HUMAN 134 102 -

Motif 3 width=10
Element Seqn Id St Int Rpt
KILEENMKLE WN7A_MOUSE 190 44 -
KILEENMKLE Q9DEB8 190 44 -
KILEENMKLE WN7A_HUMAN 190 44 -
KVLEERMKLE O42258 190 44 -
KVLEDRMKLE WN7B_MOUSE 190 44 -
KILSENLRLE O13266 189 44 -
KVLEDRMQLE WN7B_HUMAN 190 44 -

Motif 4 width=11
Element Seqn Id St Int Rpt
LSYRKPMDTDL WN7A_MOUSE 258 58 -
LSYRKPMDTDL Q9DEB8 258 58 -
LSYRKPMDTDL WN7A_HUMAN 258 58 -
RSYQKPMETDL O42258 258 58 -
RSYQKPMETDL WN7B_MOUSE 258 58 -
LSYLKPMDTDL O13266 257 58 -
RSYQKPMETDL WN7B_HUMAN 258 58 -

Motif 5 width=10
Element Seqn Id St Int Rpt
THQYARVWQC WN7A_MOUSE 315 46 -
THQYSRVWQC Q9DEB8 315 46 -
THQYARVWQC WN7A_HUMAN 315 46 -
THQYTKVWQC O42258 315 46 -
THQYTKVWQC WN7B_MOUSE 315 46 -
THQYSRVWKC O13266 314 46 -
THQYTKVWQC WN7B_HUMAN 315 46 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
GIVYLRIGGFSSVVA Q9DBY3 17 17 -
GIVYLRIGGFSSVVA WN7A_MOUSE 17 17 -
GMVYLRIGGFSSVVA Q96H90 17 17 -
GIVYIKIGGFSSVVA Q9DEB8 17 17 -
GMVCLRIGGFSSVVA WN7A_HUMAN 17 17 -
GIIYMKLGALSSVVA O42258 17 17 -
GVLYVKLGALSSVVA WN7B_MOUSE 17 17 -
GIVYIKIGGFSSVVA O13266 17 17 -
GVLYVKLGALSSVVA WN7B_HUMAN 17 17 -

Motif 2 width=12
Element Seqn Id St Int Rpt
DKEKQGQYHRDE Q9DBY3 134 102 -
DKEKQGQYHWDE WN7A_MOUSE 134 102 -
DKEKQGQYHRDE Q96H90 134 102 -
DKEKQGQYHKEE Q9DEB8 134 102 -
DKEKQGQYHRDE WN7A_HUMAN 134 102 -
DREKQGYYNQEE O42258 134 102 -
DREKQGYYNQAE WN7B_MOUSE 134 102 -
DKGKQGQFHIEE O13266 133 101 -
DREKQGYYNQAE WN7B_HUMAN 134 102 -

Motif 3 width=10
Element Seqn Id St Int Rpt
KILEENMKLE Q9DBY3 190 44 -
KILEENMKLE WN7A_MOUSE 190 44 -
KILEENMKLE Q96H90 190 44 -
KILEENMKLE Q9DEB8 190 44 -
KILEENMKLE WN7A_HUMAN 190 44 -
KVLEERMKLE O42258 190 44 -
KVLEDRMKLE WN7B_MOUSE 190 44 -
KILSENLRLE O13266 189 44 -
KVLEDRMQLE WN7B_HUMAN 190 44 -

Motif 4 width=11
Element Seqn Id St Int Rpt
LSYRKPMDTDL Q9DBY3 258 58 -
LSYRKPMDTDL WN7A_MOUSE 258 58 -
LSYRKPMDTDL Q96H90 258 58 -
LSYRKPMDTDL Q9DEB8 258 58 -
LSYRKPMDTDL WN7A_HUMAN 258 58 -
RSYQKPMETDL O42258 258 58 -
RSYQKPMETDL WN7B_MOUSE 258 58 -
LSYLKPMDTDL O13266 257 58 -
RSYQKPMETDL WN7B_HUMAN 258 58 -

Motif 5 width=10
Element Seqn Id St Int Rpt
THQYARVWQC Q9DBY3 315 46 -
THQYARVWQC WN7A_MOUSE 315 46 -
THQYARVWQC Q96H90 315 46 -
THQYSRVWQC Q9DEB8 315 46 -
THQYARVWQC WN7A_HUMAN 315 46 -
THQYTKVWQC O42258 315 46 -
THQYTKVWQC WN7B_MOUSE 315 46 -
THQYSRVWKC O13266 314 46 -
THQYTKVWQC WN7B_HUMAN 315 46 -