| Literature References | 1. GAMA-CARVALHO, M., CARVALHO, M.P., KEHLENBACH, A., VALCARCEL, J. AND
CARMO-FONSECA, M.
Nucleocytoplasmic shuttling of heterodimeric splicing factor U2AF.
J.BIOL.CHEM. 276 13104-13112 (2001).
2. HATADA, I., SUGAMA, T. AND MUKAI, T.
A new imprinted gene cloned by a methylation-sensitive genome scanning
method.
NUCLEIC ACIDS RES. 21 5577-5582 (1993).
3. PEARSALL, R.S., SHIBATA, H., BROZOWSKA, A., YOSHINO, K., OKUDA, K.,
DEJONG, P.J., PLASS, C., CHAPMAN, V.M., HAYASHIZAKI, Y. AND HELD, W.A.
Absence of imprinting in U2AFBPL, a human homologue of the imprinted mouse
gene U2afbp-rs.
BIOCHEM.BIOPHYS.RES.COMMUN. 222 171-177 (1996).
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| Documentation | The U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) is a
heterodimeric splicing factor composed of a large and a small subunit. The
large U2AF subunit recognises the intronic polypyrimidine tract, a
sequence located adjacent to the 3' splice site that serves as an important
signal for both constitutive and regulated pre-mRNA splicing. The small
subunit interacts with the 3' splice site dinucleotide AG and is essential
for regulated splicing. The subunits shuttle continuously between the
nucleus and the cytoplasm via a mechanism that involves carrier receptors
and is independent of binding to mRNA. Both subunits contain an arginine/
serine-rich (RS) domain, which acts as a nuclear localisation signal.
Furthermore, the presence of an RS domain on either subunit is sufficient
to trigger the nucleocytoplasmic import of the heterodimeric complex [1].
The human form of the U2 auxilliary factor small subunit, hU2AF35, contains
a degenerate RNA recognition motif (RRM) and a C-terminal RS domain. The
murine form has been shown to be genomically imprinted with monoallelic
expression from the paternal allele [2]. However, this is not the case in
humans [3].
U2AUXFACTOR is a 6-element fingerprint that provides a signature for the U2
auxiliary factor small subunit proteins. The fingerprint was derived from an
initial alignment of 17 sequences: the motifs were drawn from the C-terminal
portion of the alignment, motifs 2-5 spanning the RNA-binding domain. Two
iterations on SPTR40_20f were required to reach convergence, at which point
a true set comprising 22 sequences was identified. A single partial match
was also found, a human hypothetical 22.0kDa protein that matches motifs
1 and 4.
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| Sequence Titles | Q945S0 U2 AUXILIARY FACTOR SMALL SUBUNIT - Arabidopsis thaliana (Mouse-ear cress).
Q945S1 U2 AUXILIARY FACTOR SMALL SUBUNIT - Arabidopsis thaliana (Mouse-ear cress).
Q98SE2 U2SNRNP AUXILIARY FACTOR SMALL SUBUNIT - Gallus gallus (Chicken).
Q99L29 SIMILAR TO RIKEN CDNA 2010107D16 GENE - Mus musculus (Mouse).
Q9FMY5 U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT - Arabidopsis thaliana (Mouse-ear cress).
Q9N6L5 U2AF23/25 SPLICEOSOME COMPONENT - Leishmania major.
Q9S709 T22C5.10 (PUTATIVE U2 SNRNP AUXILIARY FACTOR PROTEIN) - Arabidopsis thaliana (Mouse-ear cress).
Q9SY74 F14N23.20 - Arabidopsis thaliana (Mouse-ear cress).
Q9U2U0 Y116A8C.35 PROTEIN - Caenorhabditis elegans.
Q9VR26 CG3294 PROTEIN - Drosophila melanogaster (Fruit fly).
Q9W6Q9 U2 SMALL NUCLEAR RIBONUCLEOPROTEIN AUXILIARY FACTOR SUBUNIT-RELATED PROTEIN - Fugu rubripes (Japanese pufferfish) (Takifugu rubripes).
Q9YHU4 SPLICING FACTOR U2AF35 - Fugu rubripes (Japanese pufferfish) (Takifugu rubripes).
Q9ZQW7 U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT - Oryza sativa (Rice).
Q9ZQW8 U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT - Oryza sativa (Rice).
Q9ZQW9 U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT - Oryza sativa (Rice).
U2AG_DROME Splicing factor U2af 38 kDa subunit (U2 auxiliary factor 38 kDa subunit) (U2 SNRNP auxiliary factor small subunit) - Drosophila melanogaster (Fruit fly).
U2AG_HUMAN Splicing factor U2AF 35 kDa subunit (U2 auxiliary factor 35 kDa subunit) (U2 SNRNP auxiliary factor small subunit) - Homo sapiens (Human).
U2AG_SCHPO Splicing factor U2AF 23 kDa subunit (U2 auxiliary factor 23 kDa subunit) (U2 snRNP auxiliary factor small subunit) (U2AF23) - Schizosaccharomyces pombe (Fission yeast).
U2R1_HUMAN U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit related-protein 1 - Homo sapiens (Human).
U2R1_MOUSE U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit related-protein 1 (SP2) - Mus musculus (Mouse).
U2R2_HUMAN U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit related-protein 2 - Homo sapiens (Human).
U2R2_MOUSE U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit related-protein 2 - Mus musculus (Mouse).
Q8WU68 HYPOTHETICAL 22.0 KDA PROTEIN - Homo sapiens (Human).
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