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PR01831

Identifier
TRACEAMINE1R  [View Relations]  [View Alignment]  
Accession
PR01831
No. of Motifs
6
Creation Date
20-FEB-2003
Title
Trace amine receptor 1 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01830 TRACEAMINER
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. BOROWSKY, B., ADHAM, N., JONES, K.A., RADDATZ, R., ARTYMYSHYN, R.,
OGOZALEK, K.L., DURKIN, M.M., LAKHLANI, P.P., BONINI, J.A., PATHIRANA, S.,
BOYLE, N., PU, X., KOURANOVA, E., LICHTBLAU, H., OCHOA, F.Y., BRANCHEK, 
T.A. AND GERALD, C.
Trace amines: identification of a family of mammalian G protein-coupled
receptors.
PROC.NATL.ACAD.SCI.U.S.A. 98(16) 8966-8971 (2001).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine,
para-crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
     
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of which
transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the receptors
are very similar and are believed to adopt a common structural framework
comprising 7 transmembrane (TM) helices [3-5].
 
Tyramine, beta-phenylethylamine, tryptamine and octopamine are biogenic
amines present in trace levels in mammalian nervous systems. Although some
"trace amines" have clearly defined roles as neurotransmitters in
invertebrates, the extent to which they function as true neurotransmitters
in vertebrates has remained speculative [6]. Recently, however, a novel
family of G protein-coupled receptors that share sequence similarity with
the classical amine receptors has been discovered. Members of this family
have been shown to act as receptors for tyramine and beta-phenylethylamine,
supporting a role for trace amines as neurotransmitters in vertebrates [6].
 
Trace amine receptor 1 (TA1) is potently activated by tyramine and
beta-phenylethylamine, and displays low affinity for tryptamine, octopamine
and dopamine. It is expressed at low to moderate levels in peripheral
tissues such as the stomach, kidney and lung, and within the central nervous
system is restricted predominantly to the amygdala [6].
 
TRACEAMINE1R is a 6-element fingerprint that provides a signature for
trace amine receptor 1. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those areas that
characterise trace amine receptor 1 but distinguish it from the rest of the
trace amine receptor family - motif 1 lies at the N-terminus; motif 2
encodes part of the second cytoplasmic loop, leading into TM domain 4;
motifs 3 and 4 span the N- and C-terminal portions of TM domain 5; motif 5
is located in the third external loop, leading into TM domain 7; and motif 6
resides at the C-terminus. A single iteration on SPTR40_20f was required to
reach convergence, no further sequences being identified beyond the 
starting set.
Summary Information
4 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6444444
5000000
4000000
3000000
2000000
123456
True Positives
Q8VHQ5        Q923Y8        Q923Y9        Q96RJ0        
Sequence Titles
Q8VHQ5      TRACE AMINE RECEPTOR 1 - Rattus norvegicus (Rat). 
Q923Y8 TRACE AMINE RECEPTOR 1 - Mus musculus (Mouse).
Q923Y9 TRACE AMINE RECEPTOR 1 - Rattus norvegicus (Rat).
Q96RJ0 TRACE AMINE RECEPTOR 1 (PUTATIVE CATECHOLAMINE RECEPTOR) - Homo sapiens (Human).
Scan History
SPTR40_20f 1  110  NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
WSRDVRASLYS Q8VHQ5 17 17 -
WSRDVRASLYS Q923Y9 17 17 -
WSREVQASLYS Q923Y8 17 17 -
WSNDVRASLYS Q96RJ0 18 18 -

Motif 2 width=10
Element Seqn Id St Int Rpt
PLRYKAKINL Q8VHQ5 127 99 -
PLRYKAKINL Q923Y9 127 99 -
PLRYKAKINI Q923Y8 127 99 -
PLRYKAKMNI Q96RJ0 128 99 -

Motif 3 width=14
Element Seqn Id St Int Rpt
FFSKVSGVLAFMTS Q8VHQ5 184 47 -
FFSKVSGVLAFMTS Q923Y9 184 47 -
FFSKVSGVLAFMTS Q923Y8 184 47 -
FFSKISGVLTFMTS Q96RJ0 185 47 -

Motif 4 width=12
Element Seqn Id St Int Rpt
YRIYFIAKGQAR Q8VHQ5 210 12 -
YRIYFIAKGQAR Q923Y9 210 12 -
YRIYFIAKGQAR Q923Y8 210 12 -
YRIYLIAKEQAR Q96RJ0 211 12 -

Motif 5 width=14
Element Seqn Id St Int Rpt
YVIPPTLNDTLNWF Q8VHQ5 276 54 -
YVIPPTLNDTLNWF Q923Y9 276 54 -
YVIPPSLNDALYWF Q923Y8 276 54 -
YIIPPTLNDVLIWF Q96RJ0 279 56 -

Motif 6 width=12
Element Seqn Id St Int Rpt
FQKDSSRSKLFL Q8VHQ5 321 31 -
FQKDSSRSKLFL Q923Y9 321 31 -
FQKDSSRSKLFL Q923Y8 321 31 -
FQKDSSRCKLFL Q96RJ0 324 31 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
WSRDVRASLYS Q8VHQ5 17 17 -
WSRDVRASLYS Q923Y9 17 17 -
WSREVQASLYS Q923Y8 17 17 -
WSNDVRASLYS Q96RJ0 18 18 -

Motif 2 width=10
Element Seqn Id St Int Rpt
PLRYKAKINL Q8VHQ5 127 99 -
PLRYKAKINL Q923Y9 127 99 -
PLRYKAKINI Q923Y8 127 99 -
PLRYKAKMNI Q96RJ0 128 99 -

Motif 3 width=14
Element Seqn Id St Int Rpt
FFSKVSGVLAFMTS Q8VHQ5 184 47 -
FFSKVSGVLAFMTS Q923Y9 184 47 -
FFSKVSGVLAFMTS Q923Y8 184 47 -
FFSKISGVLTFMTS Q96RJ0 185 47 -

Motif 4 width=12
Element Seqn Id St Int Rpt
YRIYFIAKGQAR Q8VHQ5 210 12 -
YRIYFIAKGQAR Q923Y9 210 12 -
YRIYFIAKGQAR Q923Y8 210 12 -
YRIYLIAKEQAR Q96RJ0 211 12 -

Motif 5 width=14
Element Seqn Id St Int Rpt
YVIPPTLNDTLNWF Q8VHQ5 276 54 -
YVIPPTLNDTLNWF Q923Y9 276 54 -
YVIPPSLNDALYWF Q923Y8 276 54 -
YIIPPTLNDVLIWF Q96RJ0 279 56 -

Motif 6 width=12
Element Seqn Id St Int Rpt
FQKDSSRSKLFL Q8VHQ5 321 31 -
FQKDSSRSKLFL Q923Y9 321 31 -
FQKDSSRSKLFL Q923Y8 321 31 -
FQKDSSRCKLFL Q96RJ0 324 31 -