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PR01809

Identifier
ANNEXINX  [View Relations]  [View Alignment]  
Accession
PR01809
No. of Motifs
3
Creation Date
13-JAN-2003
Title
Annexin type A10 signature
Database References
PRINTS; PR00196 ANNEXIN
Literature References
1. BARTON, G.J., NEWMAN, R.H., FREEMONT, P.S. AND CRUMPTON, M.J.
Amino acid sequence analysis of the annexin super-gene family of proteins.
EUR.J.BIOCHEM. 198 749-760 (1991).
 
2. BRAUN, E.L., KANG, S., NELSON, M.A. AND NATVIG, D.O.
Identification of the first fungal annexin: analysis of annexin gene
duplications and implications for eukaryotic evolution.
J.MOL.EVOL. 47 531-543 (1998).
 
3. BENZ, J. AND HOFMANN, A.
Annexins: from structure to function.
BIOL.CHEM. 378 177-183 (1997).
 
4. GEISOW, M.J.
Annexins-forms without function but not without fun.
TRENDS BIOTECHNOL. 9 180-181 (1991).
 
5. MORGAN, R.O., JENKINS, N.A., GILBERT, D.J., COPELAND, N.G., BALSARA, B.
R., TESTA, J.R. AND FERNANDEZ, M.P.
Novel human and mouse annexin A10 are linked to the genome duplications
during early chordate evolution.
GENOMICS 60 40-49 (1999).

Documentation
The annexins (or lipocortins) are a family of proteins that bind to
phospholipids in a calcium-dependent manner [1]. They are distributed
ubiquitously in different tissues and cell types of higher and lower
eukaryotes, including mammals, fish, birds, Drosophila melanogaster, Xenopus
laevis, Caenorhabtidis elegans, Dictyostelium discoideum and Neurospora 
crassa [2,3]. The plant annexins are somewhat distinct from those found in
other taxa [3].
 
Several distinct annexin subtypes exist, each of which has an amino acid
sequence consisting of an N-terminal 'arm' followed by 4 or 8 copies of a
conserved domain of 61 residues (only one of these residues, an arginine,
is conserved between all copies). The calcium binding sites are found within
the repeated domains [4]. Individual repeats (sometimes referred to as 
endonexin folds) consist of 5 alpha-helices wound into a right-handed
superhelix. The biological roles of some annexin subtypes is unclear; the
family has been linked with inhibition of phospholipase activity,
exocytosis and endoctyosis, signal transduction, organisation of the
extracellular matrix, resistance to reactive oxygen species and DNA
replication [2].
 
Human annexin A10 (annexin 14) was first identified in silico by searches of
dbEST with a number of divergent annexins [5]. The analysis revealed single
human and mouse ESTs corresponding to a novel and rarely expressed annexin
in which three of the four tetrad core repeats lack the calcium-binding 
domain. It was proposed that this subtype, together with A5 annexin, gave
rise to the type VI octad through a process of gene duplication and fusion 
in early chordate evolution [5].
 
ANNEXINX is a 3-element fingerprint that provides a signature for the
type A10 annexins. The fingerprint was derived from an initial alignment of
2 sequences: the motifs were drawn from conserved regions spanning the full 
alignment length, focusing on those sections that characterise type A10 
annexins but distinguish them from other closely related annexin subtypes -
motif 1 lies at the N-terminus; motif 2 resides between repeats 1 and 2; 
and motif 3 lies in the C-terminal region beyond the fourth repeat. Two 
iterations on SPTR40_20f were required to reach convergence, at which point
a true set comprising 3 sequences was identified. A single partial match
was also found, Q9D7W0, a mouse annexin A10 that lacks part of the 
N-terminus and therefore fails to match the first motif.
Summary Information
   3 codes involving  3 elements
1 codes involving 2 elements
Composite Feature Index
3333
2011
123
True Positives
ANXA_HUMAN    ANXA_MOUSE    Q9D272        
True Positive Partials
Codes involving 2 elements
Q9D7W0
Sequence Titles
ANXA_HUMAN  Annexin A10 (Annexin 14) - Homo sapiens (Human). 
ANXA_MOUSE Annexin A10 - Mus musculus (Mouse).
Q9D272 ANNEXIN A10 - Mus musculus (Mouse).

Q9D7W0 ANNEXIN A10 - Mus musculus (Mouse).
Scan History
SPTR40_20f 2  300  NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
MFCGEYVQGTIFPAP ANXA_MOUSE 1 1 -
MFCGDYVQGTIFPAP ANXA_HUMAN 1 1 -

Motif 2 width=14
Element Seqn Id St Int Rpt
ANREEGYSDPAMAA ANXA_MOUSE 161 145 -
GTREEGYSDPAMAA ANXA_HUMAN 161 145 -

Motif 3 width=10
Element Seqn Id St Int Rpt
AICAGDVEDY ANXA_MOUSE 315 140 -
AICAGDAEDY ANXA_HUMAN 315 140 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
MFCGEYVQGTIFPAP ANXA_MOUSE 1 1 -
MFCGEYVQGTIFPAP Q9D272 1 1 -
MFCGDYVQGTIFPAP ANXA_HUMAN 1 1 -

Motif 2 width=14
Element Seqn Id St Int Rpt
ANREEGYSDPAMAA ANXA_MOUSE 161 145 -
ANREEGYSDPAMAA Q9D272 141 125 -
GTREEGYSDPAMAA ANXA_HUMAN 161 145 -

Motif 3 width=10
Element Seqn Id St Int Rpt
AICAGDVEDY ANXA_MOUSE 315 140 -
AICAGDVEDY Q9D272 295 140 -
AICAGDAEDY ANXA_HUMAN 315 140 -