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PR01778

Identifier
KIMPTPASE  [View Relations]  [View Alignment]  
Accession
PR01778
No. of Motifs
8
Creation Date
01-JUL-2002
Title
KIM-containing protein tyrosine phosphatase signature
Database References
PRINTS; PR00700 PRTYPHPHTASE
PDB; 1JLN
SCOP; 1JLN
CATH; 1JLN
Literature References
1. FISCHER, E.H., CHARBONNEAU, H. AND TONKS, N.K.
Protein tyrosine phosphatases - A diverse family of intracellular and 
transmembrane enzymes.
SCIENCE 253 401-406 (1991).
 
2. CHARBONNEAU, H. AND TONKS, N.K.
1002 protein phosphatases.
ANNU.REV.CELL BIOL. 8 463-493 (1992).
 
3. TROWBRIDGE, I.S.
CD45 - A prototype for transmembrane protein tyrosine phosphatases.
J.BIOL.CHEM. 266 23517-23520 (1991).
 
4. TONKS, N.K. AND CHARBONNEAU, H.
Protein tyrosine dephosphorylation and signal transduction.
TRENDS BIOCHEM.SCI. 14 497-500 (1989).
 
5. HUNTER, T.
Protein-tyrosine phosphatases - the other side of the coin.
CELL 58 1013-1016 (1989).
 
6. PULIDO, R., ZUNIGA, A. AND ULLRICH, A.
PTP-SL and STEP protein tyrosine phosphatases regulate the activation of 
extracellular signal-regulated kinases ERK1 and ERK2 by association  
through a kinase interaction motif
EMBO J. 17 7337-7350 (1998).
 
7. SAXENA, M., WILLIAMS, S., BROCKDORFF, J., GILMAN, J. AND MUSTELIN, T.
Inhibition of T cell signaling by mitogen-activated protein
kinase-targeted hematopoietic tyrosine phosphatase (HePTP).
J.BIOL.CHEM. 274(17) 11693-11700 (1999).

Documentation
Tyrosine-specific protein phosphatases (PTPases) catalyse the removal of
phosphate groups attached to tyrosine residues [1-5]. The enzymes play an
important role in the control of cell growth, proliferation, differentiation
and transformation. Multiple forms of PTPase have been characterised and can
be classified into 2 groups: soluble PTPases and transmembrane (TM) receptor
proteins that contain PTPase domains.
 
The structures of receptor PTPases comprise a variable length extracellular
domain, followed by a TM region and a cytoplasmic C-terminal catalytic 
domain. The extracellular regions of some receptor PTPases house fibronectin
type III repeats, immunoglobulin-like domains, MAM domains or carbonic 
anhydrase-like domains. The cytoplasmic region generally contains 2 copies 
of the PTPase domain: the first of these is enzymatically active; the second
is inactive, but appears to affect substrate specificity in the first. 
PTPase domains contain ~300 residues, including 2 conserved cysteines, the 
second of which is required for activity. Other conserved residues in its 
immediate vicinity are also catalytically important [1-5].
 
The family of Kinase Interaction Motif (KIM)-containing phosphatases 
consists of PTP-STEP, PTP-SL and LC-PTP. Each contains a KIM in the N-
terminal portion of the molecule. The KIM sequence mediates interaction with
MAP kinases, predominantly ERK1 and ERK2. It has been experimentally shown 
that over-expression of PTP-SL down-regulates the activation of ERK2 and its 
nuclear translocation [6-7].
 
KIMPTPASE is an 8-element fingerprint that provides a signature for the KIM- 
containing protein tyrosine phosphatases. The fingerprint was derived from 
an initial alignment of 6 sequences: the motifs were drawn from conserved 
regions spanning virtually the full alignment length, focusing on those 
sections that characterise the KIM-containing PTPases but distinguish them 
from other members of the PTP family - motif 3 includes the C-terminus of
alpha-helix 1; motif 4 includes helix 5; motif 5 spans the C-terminus of
beta-strand 2 and helix 7; motif 6 encodes the N-terminal portion of helix
10; motif 7 encodes the central portion of helix 11; and motif 8 includes
the C-terminal portion of helix 13. Two iterations on SPTR40_20f were 
required to reach convergence, at which point a true set comprising 12 
sequences was identified. Several partial matches were also found: Q9BE09
is a hypothetical sequence from the crab-eating macaque, which fails to 
match the C-terminal 3 motifs; the rest are PTPases that fail to match 
motifs 2 and 5-8.
Summary Information
  12 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
0 codes involving 4 elements
2 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
81212121212121212
700000000
600000000
511111000
400000000
320220000
200000000
12345678
True Positives
O00342        O08617        PTN5_MOUSE    PTN5_RAT      
PTN7_HUMAN PTN7_RAT Q15256 Q62132
Q62695 Q63419 Q9QUH9 Q9UE65
True Positive Partials
Codes involving 5 elements
Q9BE09
Codes involving 3 elements
Q9W2F3 Q9Y0X8
Sequence Titles
O00342      CH-1PTPASE DELTA FORM - Homo sapiens (Human). 
O08617 TYROSINE PHOSPHATASE CBPTP - Rattus norvegicus (Rat).
PTN5_MOUSE Protein-tyrosine phosphatase, non-receptor type 5 (EC 3.1.3.48) (Protein-tyrosine phosphatase striatum-enriched) (STEP) (Neural- specific protein-tyrosine phosphatase) - Mus musculus (Mouse).
PTN5_RAT Protein-tyrosine phosphatase, non-receptor type 5 (EC 3.1.3.48) (Protein-tyrosine phosphatase striatum-enriched) (STEP) (Neural- specific protein-tyrosine phosphatase) - Rattus norvegicus (Rat).
PTN7_HUMAN Protein-tyrosine phosphatase, non-receptor type 7 (EC 3.1.3.48) (Protein-tyrosine phosphatase LC-PTP) (Hematopoietic protein-tyrosine phosphatase) (HEPTP) - Homo sapiens (Human).
PTN7_RAT Protein-tyrosine phosphatase, non-receptor type 7 (EC 3.1.3.48) (Protein-tyrosine phosphatase LC-PTP) (Hematopoietic protein-tyrosine phosphatase) (HEPTP) - Rattus norvegicus (Rat).
Q15256 PROTEIN-TYROSINE PHOSPHATASE R PRECURSOR (EC 3.1.3.48) (PROTEIN- TYROSINE PHOSPHATASE PCPTP1) (NC-PTPCOM1) - Homo sapiens (Human).
Q62132 PROTEIN-TYROSINE PHOSPHATASE R PRECURSOR (EC 3.1.3.48) (PROTEIN- TYROSINE-PHOSPHATASE SL) (PHOSPHOTYROSINE PHOSPHATASE) - Mus musculus (Mouse).
Q62695 PC12-PTP1 - Rattus norvegicus (Rat).
Q63419 PROTEIN TYROSINE PHOSPHATASE PCPTP1 - Rattus norvegicus (Rat).
Q9QUH9 PROTEIN TYROSINE PHOSPHATASE PBS GAMMA - Mus musculus (Mouse).
Q9UE65 CH-1PTPASE GAMMA MINUS FORM - Homo sapiens (Human).

Q9BE09 HYPOTHETICAL 42.1 KDA PROTEIN - Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey).

Q9W2F3 PTP-ER PROTEIN - Drosophila melanogaster (Fruit fly).
Q9Y0X8 PROTEIN TYROSINE PHOSPHATASE ERK (EC 3.1.3.48) - Drosophila melanogaster (Fruit fly).
Scan History
SPTR40_20f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
LQERRGSNVSLTLDM Q63419 45 45 -
LQERRGSNVSLTLDM Q15256 333 333 -
LQERRGSNVSLTLDM PTN5_MOUSE 215 215 -
LQERRGSNVSLTLDM PTN5_RAT 43 43 -
LQERRGSNVALMLDV PTN7_HUMAN 38 38 -
LQERRGSSVALMLDV PTN7_RAT 38 38 -

Motif 2 width=14
Element Seqn Id St Int Rpt
EPFVAVSTPREKVA Q63419 66 6 -
EPFVSIPTPREKVA Q15256 354 6 -
EGFGYLVSPREESA PTN5_MOUSE 237 7 -
EGFGYLVSPREESA PTN5_RAT 65 7 -
EPICSVNTPREVTL PTN7_HUMAN 59 6 -
EPICSVNTPREVTL PTN7_RAT 59 6 -

Motif 3 width=9
Element Seqn Id St Int Rpt
YLQSASRVL Q63419 82 2 -
YLQSASRIL Q15256 370 2 -
YLLSASRVL PTN5_MOUSE 253 2 -
YLLSASRVL PTN5_RAT 81 2 -
FLRTAGHPL PTN7_HUMAN 74 1 -
FLRTAGHPL PTN7_RAT 74 1 -

Motif 4 width=11
Element Seqn Id St Int Rpt
ILPNPLSRVCL Q63419 136 45 -
ILPNPLSRVCL Q15256 424 45 -
ILPNPHSRVRL PTN5_MOUSE 307 45 -
ILPNPHSRVRL PTN5_RAT 135 45 -
ILPNPQSRVCL PTN7_HUMAN 128 45 -
ILPNPQSRVCL PTN7_RAT 128 45 -

Motif 5 width=12
Element Seqn Id St Int Rpt
YIRGYSGKEKAF Q63419 163 16 -
YIRGYSGKEKAF Q15256 451 16 -
YIRGYSGEEKVY PTN5_MOUSE 334 16 -
YIRGYNGEEKVY PTN5_RAT 162 16 -
YIRGYDGKEKVY PTN7_HUMAN 154 15 -
YIRGYDGKEKVY PTN7_RAT 154 15 -

Motif 6 width=10
Element Seqn Id St Int Rpt
PLLQLMLDVE Q63419 275 100 -
PLLQLMLDVE Q15256 563 100 -
PLLHLVREVE PTN5_MOUSE 446 100 -
PLLHLVREVE PTN5_RAT 274 100 -
PLLRLVAEVE PTN7_HUMAN 266 100 -
PLLRLVAEVE PTN7_RAT 266 100 -

Motif 7 width=13
Element Seqn Id St Int Rpt
CFIATSIGCQQLK Q63419 309 24 -
CFIATSIGCQQLK Q15256 597 24 -
CFIATSICCQQLR PTN5_MOUSE 481 25 -
CFIATSICCQQLR PTN5_RAT 309 25 -
CFIATRIGCQQLK PTN7_HUMAN 300 24 -
CFIATRIGCQQLK PTN7_RAT 299 23 -

Motif 8 width=13
Element Seqn Id St Int Rpt
FVHHALCLFESRL Q63419 351 29 -
FVHHALCLYESRL Q15256 639 29 -
FVHHAMSLYEKQL PTN5_MOUSE 523 29 -
FVHHAMSLYEKQL PTN5_RAT 351 29 -
FLHHTLALYAGQL PTN7_HUMAN 342 29 -
FLHHTLALYAAQL PTN7_RAT 341 29 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
LQERRGSNVSLTLDM O08617 211 211 -
LQERRGSNVSLTLDM Q62132 332 332 -
LQERRGSNVSLTLDM Q62695 88 88 -
LQERRGSNVSLTLDM Q63419 45 45 -
LQERRGSNVSLTLDM Q9QUH9 88 88 -
LQERRGSNVSLTLDM O00342 127 127 -
LQERRGSNVSLTLDM Q15256 333 333 -
LQERRGSNVSLTLDM Q9UE65 88 88 -
LQERRGSNVSLTLDM PTN5_MOUSE 215 215 -
LQERRGSNVSLTLDM PTN5_RAT 43 43 -
LQERRGSNVALMLDV PTN7_HUMAN 38 38 -
LQERRGSSVALMLDV PTN7_RAT 38 38 -

Motif 2 width=14
Element Seqn Id St Int Rpt
EPFVAVSTPREKVA O08617 232 6 -
EPFVAVSTPREKVA Q62132 353 6 -
EPFVAVSTPREKVA Q62695 109 6 -
EPFVAVSTPREKVA Q63419 66 6 -
EPFVAVSTPREKVA Q9QUH9 109 6 -
EPFVSIPTPREKVA O00342 148 6 -
EPFVSIPTPREKVA Q15256 354 6 -
EPFVSIPTPREKVA Q9UE65 109 6 -
EGFGYLVSPREESA PTN5_MOUSE 237 7 -
EGFGYLVSPREESA PTN5_RAT 65 7 -
EPICSVNTPREVTL PTN7_HUMAN 59 6 -
EPICSVNTPREVTL PTN7_RAT 59 6 -

Motif 3 width=9
Element Seqn Id St Int Rpt
YLQSASRVL O08617 248 2 -
YLQSASRVL Q62132 369 2 -
YLQSASRVL Q62695 125 2 -
YLQSASRVL Q63419 82 2 -
YLQSASRVL Q9QUH9 125 2 -
YLQSASRIL O00342 164 2 -
YLQSASRIL Q15256 370 2 -
YLQSASRIL Q9UE65 125 2 -
YLLSASRVL PTN5_MOUSE 253 2 -
YLLSASRVL PTN5_RAT 81 2 -
FLRTAGHPL PTN7_HUMAN 74 1 -
FLRTAGHPL PTN7_RAT 74 1 -

Motif 4 width=11
Element Seqn Id St Int Rpt
ILPNPLSRVCL O08617 302 45 -
ILPNPLSRVCL Q62132 423 45 -
ILPNPLSRVCL Q62695 179 45 -
ILPNPLSRVCL Q63419 136 45 -
ILPNPLSRVCL Q9QUH9 179 45 -
ILPNPLSRVCL O00342 218 45 -
ILPNPLSRVCL Q15256 424 45 -
ILPNPLSRVCL Q9UE65 179 45 -
ILPNPHSRVRL PTN5_MOUSE 307 45 -
ILPNPHSRVRL PTN5_RAT 135 45 -
ILPNPQSRVCL PTN7_HUMAN 128 45 -
ILPNPQSRVCL PTN7_RAT 128 45 -

Motif 5 width=12
Element Seqn Id St Int Rpt
YIRGYSGKEKAF O08617 329 16 -
YIRGYSGKEKAF Q62132 450 16 -
YIRGYSGKEKAF Q62695 206 16 -
YIRGYSGKEKAF Q63419 163 16 -
YIRGYSGKEKAF Q9QUH9 206 16 -
YIRGYSGKEKAF O00342 245 16 -
YIRGYSGKEKAF Q15256 451 16 -
YIRGYSGKEKAF Q9UE65 206 16 -
YIRGYSGEEKVY PTN5_MOUSE 334 16 -
YIRGYNGEEKVY PTN5_RAT 162 16 -
YIRGYDGKEKVY PTN7_HUMAN 154 15 -
YIRGYDGKEKVY PTN7_RAT 154 15 -

Motif 6 width=10
Element Seqn Id St Int Rpt
PLLQLMLDVE O08617 441 100 -
PLLQLMLDVE Q62132 562 100 -
PLLQLMLDVE Q62695 318 100 -
PLLQLMLDVE Q63419 275 100 -
PLLQLMLDVE Q9QUH9 318 100 -
PLLQLMLDVE O00342 357 100 -
PLLQLMLDVE Q15256 563 100 -
PLLQLMLDVE Q9UE65 318 100 -
PLLHLVREVE PTN5_MOUSE 446 100 -
PLLHLVREVE PTN5_RAT 274 100 -
PLLRLVAEVE PTN7_HUMAN 266 100 -
PLLRLVAEVE PTN7_RAT 266 100 -

Motif 7 width=13
Element Seqn Id St Int Rpt
CFIATSIGCQQLK O08617 475 24 -
CFIATSIGCQQLK Q62132 596 24 -
CFIATSIGCQQLK Q62695 352 24 -
CFIATSIGCQQLK Q63419 309 24 -
CFIATSIGCQQLK Q9QUH9 352 24 -
CFIATSIGCQQLK O00342 391 24 -
CFIATSIGCQQLK Q15256 597 24 -
CFIATSIGCQQLK Q9UE65 352 24 -
CFIATSICCQQLR PTN5_MOUSE 481 25 -
CFIATSICCQQLR PTN5_RAT 309 25 -
CFIATRIGCQQLK PTN7_HUMAN 300 24 -
CFIATRIGCQQLK PTN7_RAT 299 23 -

Motif 8 width=13
Element Seqn Id St Int Rpt
FVHHALCLFESRL O08617 517 29 -
FVHHALCLFESRL Q62132 638 29 -
FVHHALCLFESRL Q62695 394 29 -
FVHHALCLFESRL Q63419 351 29 -
FVHHALCLFESRL Q9QUH9 394 29 -
FVHHALCLYESRL O00342 433 29 -
FVHHALCLYESRL Q15256 639 29 -
FVHHALCLYESRL Q9UE65 394 29 -
FVHHAMSLYEKQL PTN5_MOUSE 523 29 -
FVHHAMSLYEKQL PTN5_RAT 351 29 -
FLHHTLALYAGQL PTN7_HUMAN 342 29 -
FLHHTLALYAAQL PTN7_RAT 341 29 -