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PR01695

Identifier
IGHEPTARCPTR  [View Relations]  [View Alignment]  
Accession
PR01695
No. of Motifs
13
Creation Date
07-MAR-2002
Title
Ig-hepta receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00249 GPCRSECRETIN
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994). 
 
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.
Molecular cloning and expression of a cDNA encoding the secretin receptor.
EMBO J. 10 1635-1641 (1991).
 
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H., 
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor.
SCIENCE 254 1022-1024 (1991).
 
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F., 
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
 
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
 
6. ABE, J., SUZUKI, H., NOTOYA, M., YAMAMOTO, T. AND HIROSE, S.
Ig-hepta, a novel member of the G protein-coupled hepta-helical receptor
extracellular domain and defines a new subfamily of GPCRs.
J.BIOL.CHEM. 274(28) 19957-19964 (1999).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a
manner reminiscent of the rhodopsins and other receptors believed to inter-
act with G proteins. However, while a similar 3D framework has been
proposed to account for this, there is no significant sequence similarity
between these families: the secretin-like receptors thus bear their own
unique '7TM' signature.
 
A novel member of the secretin-like family of GPCRs has been cloned and
named Ig-hepta, due to the presence of two immunoglobulin-like repeats
in its large extracellular domain [6]. The receptor is expressed
predominantly in the lung, this expression being strongly induce
postnatally. Biochemical analysis indicates that Ig-hepta is heavily
glycosylated and exists as a disulphide-linked dimer. The receptor appears
to be localised in alveolar walls of the lungs and intercalated cells of the
kidney collecting ducts, suggesting a role in regulation of acid-base
balance [6].
 
IGHEPTARCPTR is a 13-element fingerprint that provides a signature for the
Ig-hepta receptor family. The fingerprint was derived from an initial 
alignment of 2 sequences: the motifs were drawn from conserved regions 
spanning virtually the full alignment length, focusing on those sections
that characterise the Ig-hepta receptors but distinguish them from the rest of
the secretin-like superfamily - motifs 1 and 2 lie at the N-terminus of 
the extracellular domain; motifs 3-5 span the central portion of the 
extracellular domain, between the two Ig-like domains; motifs 6 and 7 lie
between the second Ig-like domain and TM domain 1; motifs 8 and 9 span the
first and second cytoplasmic loops, respectively; motif 10 encodes the
second external loop; motif 11 spans the third cytoplasmic loop; and motifs
12 and 13 reside at the C-terminus. A single iteration on SPTR40_18f was
required to reach convergence, no further sequences being identified beyond
the starting set.
Summary Information
2 codes involving 13 elements
0 codes involving 12 elements
0 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
132222222222222
120000000000000
110000000000000
100000000000000
90000000000000
80000000000000
70000000000000
60000000000000
50000000000000
40000000000000
30000000000000
20000000000000
12345678910111213
True Positives
Q9UIZ3        Q9WVT0        
Sequence Titles
Q9UIZ3      DJ365O12.1 (KIAA0758 PROTEIN) - Homo sapiens (Human). 
Q9WVT0 SEVEN TRANSMEMBRANE RECEPTOR - Rattus norvegicus (Rat).
Scan History
SPTR40_18f 1  80   NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
AEEYTVNIEISFENASFLD Q9UIZ3 60 60 -
AEEYTVDVEISFENVSFLE Q9WVT0 60 60 -

Motif 2 width=26
Element Seqn Id St Int Rpt
CQERDVFLPGHHCSCLKELPPNGPFC Q9UIZ3 139 60 -
CQEHDSALPGRYCNCLKGLPPQGPFC Q9WVT0 135 56 -

Motif 3 width=18
Element Seqn Id St Int Rpt
EEMKVMCDNNPVSLNCCS Q9UIZ3 376 211 -
EERKVVCDNNPISLNCCS Q9WVT0 374 213 -

Motif 4 width=27
Element Seqn Id St Int Rpt
NWSKVEWKQEGKINIPGTPETDIDSSC Q9UIZ3 398 4 -
NWSRIEWKQEGKINIEGTPETDLESSC Q9WVT0 396 4 -

Motif 5 width=25
Element Seqn Id St Int Rpt
YTLKADGTQCPSGSSGTTVIYTCEF Q9UIZ3 427 2 -
YTLKADGTQCPSGSSGTTVIYTCEF Q9WVT0 425 2 -

Motif 6 width=22
Element Seqn Id St Int Rpt
NITCQDPVIGVGEPGKVIQKLC Q9UIZ3 666 214 -
NITCQDPIIGIGEPGKVIQKLC Q9WVT0 666 216 -

Motif 7 width=14
Element Seqn Id St Int Rpt
IGGTITYKCVGSQW Q9UIZ3 700 12 -
IGGTVTYKCVGSQW Q9WVT0 700 12 -

Motif 8 width=13
Element Seqn Id St Int Rpt
WKSVTKNRTSYMR Q9UIZ3 1038 324 -
WKSVTKNRTSYMR Q9WVT0 1041 327 -

Motif 9 width=17
Element Seqn Id St Int Rpt
FYRLVFILHETSRSTQK Q9UIZ3 1112 61 -
FYRLIFILHDASKSTQK Q9WVT0 1115 61 -

Motif 10 width=18
Element Seqn Id St Int Rpt
EVYTRKNVCWLNWEDTKA Q9UIZ3 1154 25 -
EVYMRKNACWLNWEDTRA Q9WVT0 1157 25 -

Motif 11 width=18
Element Seqn Id St Int Rpt
RPSIGDKPCKQEKSSLFQ Q9UIZ3 1199 27 -
RPSVGDKPGKQEKSSLFQ Q9WVT0 1202 27 -

Motif 12 width=27
Element Seqn Id St Int Rpt
KVQEALLNKFSLSRWSSQHSKSTSLGS Q9UIZ3 1272 55 -
KVQEALLHKFSLSRWSSQHSKSTSLGS Q9WVT0 1275 55 -

Motif 13 width=25
Element Seqn Id St Int Rpt
TPVFSMSSPISRRFNNLFGKTGTYN Q9UIZ3 1300 1 -
TPVFSMSSPISRRFNNLFGKTGTYN Q9WVT0 1303 1 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
AEEYTVNIEISFENASFLD Q9UIZ3 60 60 -
AEEYTVDVEISFENVSFLE Q9WVT0 60 60 -

Motif 2 width=26
Element Seqn Id St Int Rpt
CQERDVFLPGHHCSCLKELPPNGPFC Q9UIZ3 139 60 -
CQEHDSALPGRYCNCLKGLPPQGPFC Q9WVT0 135 56 -

Motif 3 width=18
Element Seqn Id St Int Rpt
EEMKVMCDNNPVSLNCCS Q9UIZ3 376 211 -
EERKVVCDNNPISLNCCS Q9WVT0 374 213 -

Motif 4 width=27
Element Seqn Id St Int Rpt
NWSKVEWKQEGKINIPGTPETDIDSSC Q9UIZ3 398 4 -
NWSRIEWKQEGKINIEGTPETDLESSC Q9WVT0 396 4 -

Motif 5 width=25
Element Seqn Id St Int Rpt
YTLKADGTQCPSGSSGTTVIYTCEF Q9UIZ3 427 2 -
YTLKADGTQCPSGSSGTTVIYTCEF Q9WVT0 425 2 -

Motif 6 width=22
Element Seqn Id St Int Rpt
NITCQDPVIGVGEPGKVIQKLC Q9UIZ3 666 214 -
NITCQDPIIGIGEPGKVIQKLC Q9WVT0 666 216 -

Motif 7 width=14
Element Seqn Id St Int Rpt
IGGTITYKCVGSQW Q9UIZ3 700 12 -
IGGTVTYKCVGSQW Q9WVT0 700 12 -

Motif 8 width=13
Element Seqn Id St Int Rpt
WKSVTKNRTSYMR Q9UIZ3 1038 324 -
WKSVTKNRTSYMR Q9WVT0 1041 327 -

Motif 9 width=17
Element Seqn Id St Int Rpt
FYRLVFILHETSRSTQK Q9UIZ3 1112 61 -
FYRLIFILHDASKSTQK Q9WVT0 1115 61 -

Motif 10 width=18
Element Seqn Id St Int Rpt
EVYTRKNVCWLNWEDTKA Q9UIZ3 1154 25 -
EVYMRKNACWLNWEDTRA Q9WVT0 1157 25 -

Motif 11 width=18
Element Seqn Id St Int Rpt
RPSIGDKPCKQEKSSLFQ Q9UIZ3 1199 27 -
RPSVGDKPGKQEKSSLFQ Q9WVT0 1202 27 -

Motif 12 width=27
Element Seqn Id St Int Rpt
KVQEALLNKFSLSRWSSQHSKSTSLGS Q9UIZ3 1272 55 -
KVQEALLHKFSLSRWSSQHSKSTSLGS Q9WVT0 1275 55 -

Motif 13 width=25
Element Seqn Id St Int Rpt
TPVFSMSSPISRRFNNLFGKTGTYN Q9UIZ3 1300 1 -
TPVFSMSSPISRRFNNLFGKTGTYN Q9WVT0 1303 1 -