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PR01678

Identifier
KIR5CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01678
No. of Motifs
6
Creation Date
13-MAR-2002
Title
Kir5 inward rectifier K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01320 KIRCHANNEL
Literature References
1. MINOR, D.L., JR., MASSELLING, S.J., JAN, Y.N. AND JAN, L.Y.
Transmembrane structure of an inwardly rectifying potassium channel.
CELL 96 879-891 (1999).
 
2. DOUPNIK, C.A., DAVIDSON, N. AND LESTER, H.A.
The inward rectifier potassium channel family.
CURR.OPIN.NEUROBIOL. 5 268-277 (1995).
 
3. REIMANN, F. AND ASHCROFT, F.M.
Inwardly rectifying potassium channels.
CURR.OPIN.CELL BIOL. 11 503-508 (1999).
 
4. LIU, Y., MCKENNA, E., FIGUEROA, D.J., BLEVINS, R. AUSTIN, C.P., 
BENNETT, P.B. AND SWANSON, R.
The human inward rectifier K(+) channel subunit Kir5.1 (KCNJ16) maps to
chromosome 17q25 and is expressed in kidney and pancreas.
CYTOGENET.CELL GENET. 90 60-63 (2000).

Documentation
Potassium channels are found in virtually all cell types. Their pore-
forming subunits fall into three structural families, i.e. those possessing
six, four and two transmembrane (TM) domains. The six-TM domain K+ channels
can be further subdivided into six families: the voltage-gated K+ channels
(Kv), the KCNQ channels, the eag-like K+ channels, and three Ca2+-activated
K+ channels. Inwardly-rectifying K+ channels (Kir) are the principal class
of two-TM domain K+ channels, and the recently-discovered two-pore domain
K+ channels, make up a family of four-TM domain K+ channels.
                  
Inwardly rectifying potassium channels (Kir) are responsible for regulating
diverse processes including: cellular excitability, vascular tone, heart
rate, renal salt flow, and insulin release [1]. To date, around twenty
members of this superfamily have been cloned, which can be grouped into six
families by sequence similarity - these are designated Kir1.x-7.x [2,3].
                  
Cloned Kir channel cDNAs encode proteins of between ~370-500 residues,
containing two predicted TM domains, with the characteristic K+ channel
pore-forming domain located between them. Both N- and C-termini are thought
to be cytoplasmic, and the N-terminus lacks a signal sequence. It is thought 
that four Kir subunits assemble to form a tetrameric channel complex, which
may be hetero- or homomeric [1].
 
Kir5 channels are significantly expressed in kidney, pancreas and thyroid
gland, suggesting that they may be involved in the regulation of fluid and
pH balance. This makes Kir5 a therapeutic target for hypertension, renal
failure and pancreatic disease [4].
 
KIR5CHANNEL is a 6-element fingerprint that provides a signature for the
Kir5 inward rectifier K+ channel. The fingerprint was derived from an
initial alignment of 3 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those 
sections that characterise Kir5 but distinguish it from other Kir channel
subtypes - motifs 1-3 reside in the N-terminus; and motifs 4-6 in the 
C-terminus. A single iteration on SPTR40_18f was required to reach 
convergence, no further sequences being identified beyond the starting set.
Summary Information
3 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6333333
5000000
4000000
3000000
2000000
123456
True Positives
IRKG_HUMAN    IRKG_MOUSE    IRKX_RAT      
Sequence Titles
IRKG_HUMAN  Inward rectifier potassium channel 16 (Potassium channel, inwardly rectifying, subfamily J, member 16) (Inward rectifier K+ channel Kir5.1) - Homo sapiens (Human). 
IRKG_MOUSE Inward rectifier potassium channel 16 (Potassium channel, inwardly rectifying, subfamily J, member 16) (Inward rectifier K+ channel Kir5.1) - Mus musculus (Mouse).
IRKX_RAT INWARD RECTIFIER POTASSIUM CHANNEL BIR9 (KIR5.1) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR40_18f 1  160  NSINGLE    
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
MSYYGSSYRIVNVDSK IRKG_MOUSE 1 1 -
MSYYGSSYRIVNVDSK IRKX_RAT 1 1 -
MSYYGSSYHIINADAK IRKG_HUMAN 1 1 -

Motif 2 width=14
Element Seqn Id St Int Rpt
PGYPPEHAIAEKRR IRKG_MOUSE 18 1 -
PGYPPEHAIAEKRR IRKX_RAT 18 1 -
PGYPPEHIIAEKRR IRKG_HUMAN 18 1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
HKDGSCNVYFKHIFGEWGS IRKG_MOUSE 38 6 -
HKDGSCNVYFKHIFGEWGS IRKX_RAT 38 6 -
HKDGSCNVYFKHIFGEWGS IRKG_HUMAN 38 6 -

Motif 4 width=17
Element Seqn Id St Int Rpt
KRKYYKVNCLQFEGSVE IRKG_MOUSE 317 260 -
KRKYYKVNCLQFEGSVE IRKX_RAT 317 260 -
KRKYYKVNCLQFEGSVE IRKG_HUMAN 317 260 -

Motif 5 width=17
Element Seqn Id St Int Rpt
RRRSFSAVAVVSSCENP IRKG_MOUSE 371 37 -
RRRSFSAVAMVSSCENP IRKX_RAT 371 37 -
RRRSFSAVAIVSSCENP IRKG_HUMAN 370 36 -

Motif 6 width=18
Element Seqn Id St Int Rpt
PYQKALLTLNRISMESQM IRKG_MOUSE 402 14 -
PYQKALLTLNRISMESQM IRKX_RAT 402 14 -
PYQKALLTLNRISVESQM IRKG_HUMAN 401 14 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
MSYYGSSYRIVNVDSK IRKG_MOUSE 1 1 -
MSYYGSSYRIVNVDSK IRKX_RAT 1 1 -
MSYYGSSYHIINADAK IRKG_HUMAN 1 1 -

Motif 2 width=14
Element Seqn Id St Int Rpt
PGYPPEHAIAEKRR IRKG_MOUSE 18 1 -
PGYPPEHAIAEKRR IRKX_RAT 18 1 -
PGYPPEHIIAEKRR IRKG_HUMAN 18 1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
HKDGSCNVYFKHIFGEWGS IRKG_MOUSE 38 6 -
HKDGSCNVYFKHIFGEWGS IRKX_RAT 38 6 -
HKDGSCNVYFKHIFGEWGS IRKG_HUMAN 38 6 -

Motif 4 width=17
Element Seqn Id St Int Rpt
KRKYYKVNCLQFEGSVE IRKG_MOUSE 317 260 -
KRKYYKVNCLQFEGSVE IRKX_RAT 317 260 -
KRKYYKVNCLQFEGSVE IRKG_HUMAN 317 260 -

Motif 5 width=17
Element Seqn Id St Int Rpt
RRRSFSAVAVVSSCENP IRKG_MOUSE 371 37 -
RRRSFSAVAMVSSCENP IRKX_RAT 371 37 -
RRRSFSAVAIVSSCENP IRKG_HUMAN 370 36 -

Motif 6 width=18
Element Seqn Id St Int Rpt
PYQKALLTLNRISMESQM IRKG_MOUSE 402 14 -
PYQKALLTLNRISMESQM IRKX_RAT 402 14 -
PYQKALLTLNRISVESQM IRKG_HUMAN 401 14 -