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PR01668

Identifier
NEUROTENSIN  [View Relations]  [View Alignment]  
Accession
PR01668
No. of Motifs
7
Creation Date
27-MAR-2002
Title
Neurotensin/neuromedin N precursor signature
Database References
Literature References
1. WATSON, S. AND ARKINSTALL, S.
Neurotensin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.199-201.
 
2. FRIRY, C., FELICIANGELI, S., RICHARD, F., KITABGI, P. AND ROVERE, C.
Production of recombinant large proneurotensin/neuromedin N-derived peptides
and characterization of their binding and biological activity.
BIOCHEM.BIOPHYS.RES.COMMUN. 290(4) 1161-1168 (2002).

Documentation
Neurotensin is a 13-residue peptide transmitter, sharing significant
similarity in its 6 C-terminal amino acid residues with several other
neuropeptides, including neuromedin N (which is derived from the same
precursor). This C-terminal region is responsible for the full biological
activity, the N-terminal portion having a modulatory role [1]. 
 
Neurotensin is distributed throughout the central nervous system, with
highest levels in the hypothalamus, amygdala and nucleus accumbens. It
induces a variety of effects, including: analgesia, hypothermia and 
increased locomotor activity. It is also involved in regulation of dopamine
pathways. In the periphery, neurotensin is found in endocrine cells of the
small intestine, where it leads to secretion and smooth muscle contraction
[1,2]. The neurotensin/neuromedin N precursor can also be processed to
produce large 125-138 amino acid peptides with the neurotensin or neuromedin
N sequence at their C-terminus [2]. These large peptides appear to be less
potent than their smaller counterparts, but are also less sensitive to 
degradation and may represent endogenous, long-lasting activators in a
number of pathophysiological situations [2].
 
NEUROTENSIN is a 7-element fingerprint that provides a signature for the
neurotensin/neuromedin N precursor. The fingerprint was derived from an
initial alignment of 5 sequences: the motifs were drawn from conserved
regions spanning the full alignment length - motif 1 encodes the putative
signal peptide; motifs 2-6 span the region of the precursor that encodes
large neuromedin N, with motif 6 containing neuromedin N itself; and motif 7
encodes neurotensin and the C-terminal tail. A single iteration on
SPTR40_18f was required to reach convergence, no further sequences being
identified beyond the starting set.
Summary Information
5 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
75555555
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
NEUT_BOVIN    NEUT_CANFA    NEUT_HUMAN    NEUT_RAT      
Q9D3P9
Sequence Titles
NEUT_BOVIN  Neurotensin/neuromedin N precursor (NT/NMN) - Bos taurus (Bovine). 
NEUT_CANFA Neurotensin/neuromedin N precursor (NT/NMN) - Canis familiaris (Dog).
NEUT_HUMAN Neurotensin/neuromedin N precursor (NT/NMN) - Homo sapiens (Human).
NEUT_RAT Neurotensin/neuromedin N precursor (NT/NMN) - Rattus norvegicus (Rat).
Q9D3P9 5033428E16RIK PROTEIN (NEUROTENSIN/NEUROMEDIN N PRECURSOR) - Mus musculus (Mouse).
Scan History
SPTR40_18f 1  200  NSINGLE    
Initial Motifs
Motif 1  width=24
Element Seqn Id St Int Rpt
GMKIQLVCMILLAFSSWSLCSDSE NEUT_BOVIN 3 3 -
GMKIQLVCMILLAFSSWSLCSDSE NEUT_CANFA 4 4 -
GMKIQLVCMLLLAFSSWSLCSDSE NEUT_HUMAN 4 4 -
GMNLQLVCLTLLAFSSWSLCSDSE Q9D3P9 3 3 -
GMNLQLVCLTLLAFSSWSLCSDSE NEUT_RAT 3 3 -

Motif 2 width=21
Element Seqn Id St Int Rpt
EEMKALETDLLTNMHTSKISK NEUT_BOVIN 27 0 -
EEMKALEADLLTNMHTSKISK NEUT_CANFA 28 0 -
EEMKALEADFLTNMHTSKISK NEUT_HUMAN 28 0 -
EDVRALEADLLTNMHTSKISK Q9D3P9 27 0 -
EDVRALEADLLTNMHASKVSK NEUT_RAT 27 0 -

Motif 3 width=27
Element Seqn Id St Int Rpt
SWKMSLLNVCSLINNLNSQAEETGEFH NEUT_BOVIN 52 4 -
SWKMTLLNVCSFVNNLNSQAEETGEFR NEUT_CANFA 53 4 -
SWKMTLLNVCSLVNNLNSPAEETGEVH NEUT_HUMAN 53 4 -
SWKMTLLNVCSLINNVNSPAEEAGDMH Q9D3P9 52 4 -
SWKMTLLNVCSLINNLNSAAEEAGEMR NEUT_RAT 52 4 -

Motif 4 width=27
Element Seqn Id St Int Rpt
EEELITRRKFPAALDGFSLEAMLTIYQ NEUT_BOVIN 79 0 -
EEELITRRKFPTALDGFSLEAMLTIYQ NEUT_CANFA 80 0 -
EEELVARRKLPTALDGFSLEAMLTIYQ NEUT_HUMAN 80 0 -
DDDLVGKRKLPLVLDGFSLEAMLTIFQ Q9D3P9 79 0 -
DDDLVAKRKLPLVLDDFSLEALLTVFQ NEUT_RAT 79 0 -

Motif 5 width=25
Element Seqn Id St Int Rpt
KICHSRAFQHWELIQEDILDAGNDK NEUT_BOVIN 108 2 -
KICHSRAFQQWELIQEDVLDAGNDK NEUT_CANFA 109 2 -
KICHSRAFQHWELIQEDILDTGNDK NEUT_HUMAN 109 2 -
KICRSRAFQHWEIIQEDILDNVNDK Q9D3P9 108 2 -
KICRSRAFQHWEIIQEDILDHGNEK NEUT_RAT 108 2 -

Motif 6 width=20
Element Seqn Id St Int Rpt
NEKEEVIKRKIPYILKRQLY NEUT_BOVIN 133 0 -
NEKEEVIKRKIPYILKRQLY NEUT_CANFA 134 0 -
NGKEEVIKRKIPYILKRQLY NEUT_HUMAN 134 0 -
NEKEEVIKRKIPYILKRQLY Q9D3P9 133 0 -
TEKEEVIKRKIPYILKRQLY NEUT_RAT 133 0 -

Motif 7 width=17
Element Seqn Id St Int Rpt
ENKPRRPYILKRGSYYY NEUT_BOVIN 153 0 -
ENKPRRPYILKRGSYYY NEUT_CANFA 154 0 -
ENKPRRPYILKRDSYYY NEUT_HUMAN 154 0 -
ENKPRRPYILKRGSYYY Q9D3P9 153 0 -
ENKPRRPYILKRASYYY NEUT_RAT 153 0 -
Final Motifs
Motif 1  width=24
Element Seqn Id St Int Rpt
GMKIQLVCMILLAFSSWSLCSDSE NEUT_BOVIN 3 3 -
GMKIQLVCMILLAFSSWSLCSDSE NEUT_CANFA 4 4 -
GMKIQLVCMLLLAFSSWSLCSDSE NEUT_HUMAN 4 4 -
GMNLQLVCLTLLAFSSWSLCSDSE Q9D3P9 3 3 -
GMNLQLVCLTLLAFSSWSLCSDSE NEUT_RAT 3 3 -

Motif 2 width=21
Element Seqn Id St Int Rpt
EEMKALETDLLTNMHTSKISK NEUT_BOVIN 27 0 -
EEMKALEADLLTNMHTSKISK NEUT_CANFA 28 0 -
EEMKALEADFLTNMHTSKISK NEUT_HUMAN 28 0 -
EDVRALEADLLTNMHTSKISK Q9D3P9 27 0 -
EDVRALEADLLTNMHASKVSK NEUT_RAT 27 0 -

Motif 3 width=27
Element Seqn Id St Int Rpt
SWKMSLLNVCSLINNLNSQAEETGEFH NEUT_BOVIN 52 4 -
SWKMTLLNVCSFVNNLNSQAEETGEFR NEUT_CANFA 53 4 -
SWKMTLLNVCSLVNNLNSPAEETGEVH NEUT_HUMAN 53 4 -
SWKMTLLNVCSLINNVNSPAEEAGDMH Q9D3P9 52 4 -
SWKMTLLNVCSLINNLNSAAEEAGEMR NEUT_RAT 52 4 -

Motif 4 width=27
Element Seqn Id St Int Rpt
EEELITRRKFPAALDGFSLEAMLTIYQ NEUT_BOVIN 79 0 -
EEELITRRKFPTALDGFSLEAMLTIYQ NEUT_CANFA 80 0 -
EEELVARRKLPTALDGFSLEAMLTIYQ NEUT_HUMAN 80 0 -
DDDLVGKRKLPLVLDGFSLEAMLTIFQ Q9D3P9 79 0 -
DDDLVAKRKLPLVLDDFSLEALLTVFQ NEUT_RAT 79 0 -

Motif 5 width=25
Element Seqn Id St Int Rpt
KICHSRAFQHWELIQEDILDAGNDK NEUT_BOVIN 108 2 -
KICHSRAFQQWELIQEDVLDAGNDK NEUT_CANFA 109 2 -
KICHSRAFQHWELIQEDILDTGNDK NEUT_HUMAN 109 2 -
KICRSRAFQHWEIIQEDILDNVNDK Q9D3P9 108 2 -
KICRSRAFQHWEIIQEDILDHGNEK NEUT_RAT 108 2 -

Motif 6 width=20
Element Seqn Id St Int Rpt
NEKEEVIKRKIPYILKRQLY NEUT_BOVIN 133 0 -
NEKEEVIKRKIPYILKRQLY NEUT_CANFA 134 0 -
NGKEEVIKRKIPYILKRQLY NEUT_HUMAN 134 0 -
NEKEEVIKRKIPYILKRQLY Q9D3P9 133 0 -
TEKEEVIKRKIPYILKRQLY NEUT_RAT 133 0 -

Motif 7 width=17
Element Seqn Id St Int Rpt
ENKPRRPYILKRGSYYY NEUT_BOVIN 153 0 -
ENKPRRPYILKRGSYYY NEUT_CANFA 154 0 -
ENKPRRPYILKRDSYYY NEUT_HUMAN 154 0 -
ENKPRRPYILKRGSYYY Q9D3P9 153 0 -
ENKPRRPYILKRASYYY NEUT_RAT 153 0 -