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PR01655

Identifier
UDPGLUCOSER  [View Relations]  [View Alignment]  
Accession
PR01655
No. of Motifs
7
Creation Date
19-DEC-2001
Title
UDP-glucose receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. CHAMBERS, J.K., MACDONALD, L.E., SARAU, H.M., AMES, R.S., FREEMAN, K.,
FOLEY, J.J., ZHU, Y., MCLAUGHLIN, M.M., MURDOCK, P., MCMILLAN, L., TRILL, J.,
SWIFT, A., AIYAR, N., TAYLOR, P., VAWTER, L., NAHEED, S., SZEKERES, P.,
HERVIEU, G., SCOTT, C., WATSON, J.M., MURPHY, A.J., DUZIC, E., KLEIN, C.,
BERGSMA, D.J., WILSON, S. AND LIVI, G.P.
A G protein-coupled receptor for UDP-glucose.
J.BIOL.CHEM. 275(15) 10767-10771 (2000).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of which
transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5]. 
   
In addition to its well established role in the enzymatic biosynthesis of
carbohydrates, UDP-glucose also acts as an extracellular signalling molecule
through binding to cell-surface receptors [6]. An orphan G protein-coupled
receptor, previously known as KIAA0001 or GPR105, has been identified as a
receptor for UDP-glucose. The receptor is expressed widely, with highest
levels in the placenta, adipose tissue, stomach and intestine, and moderate
levels in various regions of the brain, spleen, lung and heart. Signal
transduction from the receptor is through pertussis toxin-sensitive G
proteins, probably of the Gi class [6.]
 
UDPGLUCOSER is a 7-element fingerprint that provides a signature for the
UDP-glucose receptor. The fingerprint was derived from an initial alignment
of 3 sequences: the motifs were drawn from conserved sections within N- and
C-terminal and loop regions, focusing on those areas of the alignment that
characterise the UDP-glucose receptors but distinguish them from the rest of
the rhodopsin-like superfamily - motifs 1 resides at the N-terminus; motif 2
spans the first cytoplasmic loop; motif 3 encodes the second cytoplasmic
loop; motif 4 lies in the second external loop; motifs 5 and 6 encompass 
the third cytoplasmic loop and the adjacent portions of TM domains 5 and 6;
and motif 7 lies in the third external loop. A single iteration on
SPTR40_18f was required to reach convergence, no further sequences being 
identified beyond the starting set.
Summary Information
3 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
73333333
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
KI01_HUMAN    KI01_RAT      Q9ESG6        
Sequence Titles
KI01_HUMAN  Probable G protein-coupled receptor KIAA0001 - Homo sapiens (Human). 
KI01_RAT Probable G protein-coupled receptor VTR 15-20 - Rattus norvegicus (Rat).
Q9ESG6 7 TRANSMEMBRANE G-PROTEIN COUPLED RECEPTOR - Mus musculus (Mouse).
Scan History
SPTR40_18f 1  50   NSINGLE    
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
NSTTTDPPNQPCSWNTLITKQ Q9ESG6 3 3 -
NTTTTEPPKQPCTRNTLITQQ KI01_RAT 3 3 -
NSTSTQPPDESCSQNLLITQQ KI01_HUMAN 3 3 -

Motif 2 width=13
Element Seqn Id St Int Rpt
WIFFYVPSSKSFI Q9ESG6 45 21 -
WIFFYVPSSKSFI KI01_RAT 45 21 -
WIFFYVPSSKSFI KI01_HUMAN 45 21 -

Motif 3 width=19
Element Seqn Id St Int Rpt
YKIVKPLLTSIVQSVNYSK Q9ESG6 121 63 -
YKIVKPLLVSIVQSVNYSK KI01_RAT 121 63 -
YKIVKPLWTSFIQSVSYSK KI01_HUMAN 121 63 -

Motif 4 width=20
Element Seqn Id St Int Rpt
TNQGVKEVTKIQCMELKNEL Q9ESG6 160 20 -
TNQSVKDVTNIQCMELKNEL KI01_RAT 160 20 -
TNQSVREVTQIKCIELKSEL KI01_HUMAN 160 20 -

Motif 5 width=14
Element Seqn Id St Int Rpt
TRKIFKSHLKSRKN Q9ESG6 210 30 -
TRKIFKSHLKSRKN KI01_RAT 210 30 -
TKKIFKSHLKSSRN KI01_HUMAN 210 30 -

Motif 6 width=14
Element Seqn Id St Int Rpt
STSVKRKSSRNIFS Q9ESG6 224 0 -
SISVKRKSSRNIFS KI01_RAT 224 0 -
STSVKKKSSRNIFS KI01_HUMAN 224 0 -

Motif 7 width=18
Element Seqn Id St Int Rpt
EGHYSCRTKETLLYAKEF Q9ESG6 262 24 -
EGHYSCQAKETLLYTKEF KI01_RAT 262 24 -
EAHYSCQSKEILRYMKEF KI01_HUMAN 262 24 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
NSTTTDPPNQPCSWNTLITKQ Q9ESG6 3 3 -
NTTTTEPPKQPCTRNTLITQQ KI01_RAT 3 3 -
NSTSTQPPDESCSQNLLITQQ KI01_HUMAN 3 3 -

Motif 2 width=13
Element Seqn Id St Int Rpt
WIFFYVPSSKSFI Q9ESG6 45 21 -
WIFFYVPSSKSFI KI01_RAT 45 21 -
WIFFYVPSSKSFI KI01_HUMAN 45 21 -

Motif 3 width=19
Element Seqn Id St Int Rpt
YKIVKPLLTSIVQSVNYSK Q9ESG6 121 63 -
YKIVKPLLVSIVQSVNYSK KI01_RAT 121 63 -
YKIVKPLWTSFIQSVSYSK KI01_HUMAN 121 63 -

Motif 4 width=20
Element Seqn Id St Int Rpt
TNQGVKEVTKIQCMELKNEL Q9ESG6 160 20 -
TNQSVKDVTNIQCMELKNEL KI01_RAT 160 20 -
TNQSVREVTQIKCIELKSEL KI01_HUMAN 160 20 -

Motif 5 width=14
Element Seqn Id St Int Rpt
TRKIFKSHLKSRKN Q9ESG6 210 30 -
TRKIFKSHLKSRKN KI01_RAT 210 30 -
TKKIFKSHLKSSRN KI01_HUMAN 210 30 -

Motif 6 width=14
Element Seqn Id St Int Rpt
STSVKRKSSRNIFS Q9ESG6 224 0 -
SISVKRKSSRNIFS KI01_RAT 224 0 -
STSVKKKSSRNIFS KI01_HUMAN 224 0 -

Motif 7 width=18
Element Seqn Id St Int Rpt
EGHYSCRTKETLLYAKEF Q9ESG6 262 24 -
EGHYSCQAKETLLYTKEF KI01_RAT 262 24 -
EAHYSCQSKEILRYMKEF KI01_HUMAN 262 24 -