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PR01612

Identifier
CRFFAMILY  [View Relations]  [View Alignment]  
Accession
PR01612
No. of Motifs
3
Creation Date
14-DEC-2001
Title
Corticotropin-releasing factor family signature
Database References

PROSITE; PS00511 CRF
PFAM; PF00473 CRF
INTERPRO; IPR003620
Literature References
1. LOVEJOY, D.A. AND BALMENT, R.J.
Evolution and physiology of the corticotropin-releasing factor (CRF) family
of neuropeptides in vertebrates.
GEN.COMP.ENDOCRINOL. 115(1) 1-22 (1999).
 
2. SMAGIN, G.N., HEINRICHS, S.C. AND DUNN, A.J.
The role of CRH in behavioral responses to stress.
PEPTIDES 22 713-724 (2001).

Documentation
Corticotropin-releasing factor (CRF), urotensin-I, urocortin and sauvagine
form a family of related neuropeptides in vertebrates. The family can be
grouped into 2 separate paralogous lineages, with urotensin-I, urocortin and
sauvagine in one group and CRF forming the other group. Urocortin and
sauvagine appear to represent orthologues of fish urotensin-I in mammals and
amphibians, respectively. The peptides have a variety of physiological
effects on stress and anxiety, vasoregulation, thermoregulation, growth and
metabolism, metamorphosis and reproduction in various species, and are all
released as preprohormones [1].
 
CRF is found mainly in the paraventricular nucleus of the mammalian hypo-
thalamus (PVN), where it is released into the portal vessels. From here, CRF
is transported to the anterior pituitary, stimulating adrenocorticotropic
hormone (ACTH) release via CRF type 1 receptors, thereby activating the
hypothalamo-pituitary-adrenocortical axis (HPA) and thus glucocorticoid
release. Urocortin is also found in mammalian brain; however, the
distribution of its mRNA is quite different from CRF, highest expression 
levels being found in the Edinger-Westphal (EW) nucleus. In addition, 
urocortin exhibits a much greater potency at CRF type 2 receptors, which 
are absent from the anterior pituitary [2]. Urotensin-I is released
from the urophysis of fish, and produces ACTH and subsequent cortisol 
release in vivo. The nonhormonal portion of the prohormone is thought to be
the urotensin binding protein (urophysin). Sauvagine, isolated from frog 
skin, has a potent hypotensive and diuretic effect.
 
CRFFAMILY is a 3-element fingerprint that provides a signature for the
corticotropin-releasing factor related neuropeptides. The fingerprint was
derived from an initial alignment of 8 sequences: the motifs were drawn from
conserved regions spanning the C-terminal peptide portion of the alignment. 
Two iterations on SPTR40_18f were required to reach convergence, at which 
point a true set comprising 17 sequences was identified.
Summary Information
17 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3171717
2000
123
True Positives
CRF1_CATCO    CRF2_CATCO    CRF_CARAU     CRF_HUMAN     
CRF_PIG CRF_RAT CRF_SHEEP CRF_XENLA
Q9I9D0 SAUV_PHYSA UR1_CARAU UR1_CATCO
UR1_CYPCA UR1_ONCMY UROC_HUMAN UROC_MOUSE
UROC_RAT
Sequence Titles
CRF1_CATCO  Corticoliberin 1 precursor (Corticotropin-releasing factor 1) (CRF 1) (Corticotropin releasing hormone 1) - Catostomus commersoni (White sucker). 
CRF2_CATCO Corticoliberin 2 precursor (Corticotropin-releasing factor 2) (CRF 2) (Corticotropin releasing hormone 2) - Catostomus commersoni (White sucker).
CRF_CARAU Corticoliberin precursor (Corticotropin-releasing factor) (CRF) (Corticotropin releasing hormone) - Carassius auratus (Goldfish).
CRF_HUMAN Corticoliberin precursor (Corticotropin-releasing factor) (CRF) (Corticotropin releasing hormone) - Homo sapiens (Human).
CRF_PIG Corticoliberin precursor (Corticotropin-releasing factor) (CRF) (Corticotropin releasing hormone) - Sus scrofa (Pig).
CRF_RAT Corticoliberin precursor (Corticotropin-releasing factor) (CRF) (Corticotropin releasing hormone) - Rattus norvegicus (Rat).
CRF_SHEEP Corticoliberin precursor (Corticotropin-releasing factor) (CRF) (Corticotropin releasing hormone) (Endorpholiberin) - Ovis aries (Sheep).
CRF_XENLA Corticoliberin precursor (Corticotropin-releasing factor) (CRF) (Corticotropin releasing hormone) - Xenopus laevis (African clawed frog).
Q9I9D0 CORTICOTROPIN-RELEASING HORMONE (CRH) PRECURSOR - Oreochromis mossambicus (Mozambique tilapia) (Tilapia mossambica).
SAUV_PHYSA Sauvagine (SVG) - Phyllomedusa sauvagei (Sauvage's leaf frog).
UR1_CARAU Urotensin I precursor - Carassius auratus (Goldfish).
UR1_CATCO Urotensin I - Catostomus commersoni (White sucker).
UR1_CYPCA Urotensin I precursor - Cyprinus carpio (Common carp).
UR1_ONCMY Urotensin I precursor - Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
UROC_HUMAN Urocortin precursor - Homo sapiens (Human).
UROC_MOUSE Urocortin precursor - Mus musculus (Mouse).
UROC_RAT Urocortin precursor (Corticotensin) - Rattus norvegicus (Rat).
Scan History
SPTR40_18f 2  300  NSINGLE    
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
EEPPISLDLTFHLL CRF_RAT 146 146 -
EEPPISLDLTFHLL CRF_CARAU 121 121 -
EEPPISLDLTFHLL CRF2_CATCO 121 121 -
DDPPISIDLTFHLL UR1_ONCMY 124 124 -
DDPPISIDLTFHLL UR1_CATCO 2 2 -
EDPPISLDLTFHLL Q9I9D0 126 126 -
DDPPLSIDLTFHLL UROC_RAT 81 81 -
QGPPISIDLSLELL SAUV_PHYSA 1 1 -

Motif 2 width=13
Element Seqn Id St Int Rpt
REVLEMARAEQLA CRF_RAT 160 0 -
REVLEMARAEQMA CRF_CARAU 135 0 -
REVLEMARAEQLV CRF2_CATCO 135 0 -
RNMIEMARIESQK UR1_ONCMY 138 0 -
RNMIEMARIENER UR1_CATCO 16 0 -
REMMEMSRAEQLA Q9I9D0 140 0 -
RTLLELARTQSQR UROC_RAT 95 0 -
RKMIEIEKQEKEK SAUV_PHYSA 15 0 -

Motif 3 width=13
Element Seqn Id St Int Rpt
QQAHSNRKLMEII CRF_RAT 173 0 -
QQAHSNRKMMEIF CRF_CARAU 148 0 -
QQAHSNRKMMEIF CRF2_CATCO 148 0 -
EQAELNRKYLDEV UR1_ONCMY 151 0 -
EQAGLNRKYLDEV UR1_CATCO 29 0 -
QQAQNNRRMMELF Q9I9D0 153 0 -
ERAEQNRIIFDSV UROC_RAT 108 0 -
QQAANNRLLLDTI SAUV_PHYSA 28 0 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
EEPPISLDLTFHLL CRF_HUMAN 155 155 -
EEPPISLDLTFHLL CRF_PIG 150 150 -
EEPPISLDLTFHLL CRF_RAT 146 146 -
EEPPISLDLTFHLL CRF_XENLA 121 121 -
EEPPISLDLTFHLL CRF1_CATCO 121 121 -
EEPPISLDLTFHLL CRF_CARAU 121 121 -
EEPPISLDLTFHLL CRF2_CATCO 121 121 -
QEPPISLDLTFHLL CRF_SHEEP 149 149 -
DDPPISIDLTFHLL UR1_CARAU 104 104 -
DDPPISIDLTFHLL UR1_CYPCA 104 104 -
DDPPISIDLTFHLL UR1_ONCMY 124 124 -
DDPPISIDLTFHLL UR1_CATCO 2 2 -
EDPPISLDLTFHLL Q9I9D0 126 126 -
DDPPLSIDLTFHLL UROC_MOUSE 81 81 -
DDPPLSIDLTFHLL UROC_RAT 81 81 -
DNPSLSIDLTFHLL UROC_HUMAN 83 83 -
QGPPISIDLSLELL SAUV_PHYSA 1 1 -

Motif 2 width=13
Element Seqn Id St Int Rpt
REVLEMARAEQLA CRF_HUMAN 169 0 -
REVLEMARAEQLA CRF_PIG 164 0 -
REVLEMARAEQLA CRF_RAT 160 0 -
REVLEMARAEQIA CRF_XENLA 135 0 -
REVLEMARAEQLA CRF1_CATCO 135 0 -
REVLEMARAEQMA CRF_CARAU 135 0 -
REVLEMARAEQLV CRF2_CATCO 135 0 -
REVLEMTKADQLA CRF_SHEEP 163 0 -
RNMIEMARNENQR UR1_CARAU 118 0 -
RNMIEMARNENQR UR1_CYPCA 118 0 -
RNMIEMARIESQK UR1_ONCMY 138 0 -
RNMIEMARIENER UR1_CATCO 16 0 -
REMMEMSRAEQLA Q9I9D0 140 0 -
RTLLELARTQSQR UROC_MOUSE 95 0 -
RTLLELARTQSQR UROC_RAT 95 0 -
RTLLELARTQSQR UROC_HUMAN 97 0 -
RKMIEIEKQEKEK SAUV_PHYSA 15 0 -

Motif 3 width=13
Element Seqn Id St Int Rpt
QQAHSNRKLMEII CRF_HUMAN 182 0 -
QQAHSNRKLMEII CRF_PIG 177 0 -
QQAHSNRKLMEII CRF_RAT 173 0 -
QQAHSNRKLMDII CRF_XENLA 148 0 -
QQAHSNRKMMEIF CRF1_CATCO 148 0 -
QQAHSNRKMMEIF CRF_CARAU 148 0 -
QQAHSNRKMMEIF CRF2_CATCO 148 0 -
QQAHSNRKLLDIA CRF_SHEEP 176 0 -
EQAGLNRKYLDEV UR1_CARAU 131 0 -
EQAGLNRKYLDEV UR1_CYPCA 131 0 -
EQAELNRKYLDEV UR1_ONCMY 151 0 -
EQAGLNRKYLDEV UR1_CATCO 29 0 -
QQAQNNRRMMELF Q9I9D0 153 0 -
ERAEQNRIIFDSV UROC_MOUSE 108 0 -
ERAEQNRIIFDSV UROC_RAT 108 0 -
ERAEQNRIIFDSV UROC_HUMAN 110 0 -
QQAANNRLLLDTI SAUV_PHYSA 28 0 -