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PR01608

Identifier
BACINVASINC  [View Relations]  [View Alignment]  
Accession
PR01608
No. of Motifs
8
Creation Date
23-DEC-2001
Title
Salmonella/Shigella invasin protein C signature
Database References
Literature References
1. CORNELIUS, G. R. AND VAN GIJSEGEM, F. 
Assembly and function of type III secretory systems. 
ANNU.REV.MICROBIOL. 54 735-774 (2000). 
 
2. VENKATESAN, M.M., BUYSSE, J.M. AND KOPECKO, D.J.
Characterization of invasion plasmid antigen genes (ipaBCD) from Shigella 
flexneri.
PROC.NATL.ACAD.SCI.U.S.A. 85 9317-21 (1988).
 
3. KANIGA, K., TUCKER, S., TROLLINGER, D. AND GALAN, J.E.
Homologs of the Shigella IpaB and IpaC invasins are required for Salmonella 
typhimurium entry into cultured epithelial cells.
J.BACTERIOL. 177 3965-3971 (1995).
 
4. CARLSON, S.A. AND JONES, B.D.
Inhibition of Salmonella typhimurium invasion by host cell expression of 
secreted bacterial invasion proteins.
INFECT.IMMUN. 66 5295-5300 (1998).

Documentation
Some Gram-negative animal enteropathogens express a specialised secretion 
system to directly "inject" exotoxins into the cytoplasm of host cells. 
Dubbed the type III secretion system, it is of specific interest to 
researchers, as the components of such a system are only expressed in 
pathogenic strains [1]. The system is composed of structural proteins and 
exotoxin effectors; these are often encoded on large virulence plasmids
or on the bacterial chromosome itself [1].
 
The Shigella flexneri invasion plasmid antigen (ipa) genes are found on such 
a plasmid, and are highly regulated [2]. Homologues of the ipa genes 
(SipC/SspC) have been found in Salmonella typhimurium [3], and both 
pathogens utilise their type III system to translocate their invasive 
effectors. These are responsible for interacting directly with the host 
eukaryotic cell. IpaC can activate cellular kinase activity once in the 
host cytoplasm, and thus promotes cellular uptake of Shigella flexneri [4]. 
In addition, it has been found that SipC interacts with another Salmonella 
protein, SipA, to enhance its reorganisation of the host cell actin 
cytoskeleton, thereby facilitating cellular uptake of the Salmonella 
bacterium by the eukaryotic cell [4].
 
BACINVASINC is an 8-element fingerprint that provides a signature for 
the Salmonella/Shigella invasin protein C family. The fingerprint was 
derived from an initial alignment of 4 sequences: the motifs were drawn
from conserved regions spanning virtually the full alignment length. Two 
iterations on SPTR40_18f were required to reach convergence, at which point
a true set comprising 6 sequences were identified. 
Summary Information
6 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
866666666
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
IPAC_SHIDY    IPAC_SHIFL    Q56020        Q56025        
Q56135 Q9AJW2
Sequence Titles
IPAC_SHIDY  42 kDa membrane antigen precursor - Shigella dysenteriae. 
IPAC_SHIFL 42 kDa membrane antigen precursor - Shigella flexneri.
Q56020 SIPC - Salmonella typhimurium.
Q56025 SSPC - Salmonella typhimurium.
Q56135 SPAT, SIPB, SIPC, SIPD AND SIPA GENES - Salmonella typhi.
Q9AJW2 IPAC, SECRETED BY THE MXI-SPA SECRETION MACHINERY, REQUIRED FOR ENTRY INTO EPITHELIAL CELLS - Shigella flexneri.
Scan History
SPTR40_18f 2  75   NSINGLE    
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
TASQSVSAKDILNSIGISSSKVS Q56020 25 25 -
TASQSVSAKDILNSIGISSSKVS Q56025 25 25 -
TKPTQTLYTDISTKQTQSSSETQ IPAC_SHIFL 25 25 -
TKSTQILYTDISTKQTQSSSETQ IPAC_SHIDY 25 25 -

Motif 2 width=25
Element Seqn Id St Int Rpt
TLSAPAPGVLTQTPGTITSSLKASI Q56020 54 6 -
TLSAPAPGVLTQTPGTITSFLKASI Q56025 54 6 -
QIAAHIPLNVGKNPVLTTTLNDDQL IPAC_SHIFL 54 6 -
QIAAHIPLNVGKNPVLTTTLNDDQL IPAC_SHIDY 54 6 -

Motif 3 width=29
Element Seqn Id St Int Rpt
DISGMSSSAVALLAAANTLMLTLNQADSK Q56020 118 39 -
DISGMSSSAVALLAAANTLMLTLNQADSK Q56025 118 39 -
DISSLSSNAVSLIISVAVLLSALRTAETK IPAC_SHIFL 117 38 -
DISSLSSNAVSLIISVAVLLSALRTAETK IPAC_SHIDY 117 38 -

Motif 4 width=20
Element Seqn Id St Int Rpt
LSLVSFDAAKTTASSMMREG Q56020 151 4 -
LSLVSFDAAKTTASSMMREG Q56025 151 4 -
LSLIAFDATKSAAENIVRQG IPAC_SHIFL 150 4 -
LSLIAFDATKSAAENIVRQG IPAC_SHIDY 150 4 -

Motif 5 width=24
Element Seqn Id St Int Rpt
GMNALSGSISQSALQLGITGVGAK Q56020 170 -1 -
GMNALSGSISQSALQLGITGVGAK Q56025 170 -1 -
GLAALSSSITGAVTQVGITGIGAK IPAC_SHIFL 169 -1 -
GLAALSSSITGAVTQVGITGIGAK IPAC_SHIDY 169 -1 -

Motif 6 width=22
Element Seqn Id St Int Rpt
GLQNERGALKHNAAKIDKLTTE Q56020 198 4 -
GLQNERGALKHNAAKIDKLTTE Q56025 198 4 -
GISDQKGALRKNLATAQSLEKE IPAC_SHIFL 197 4 -
GISDQKGALRKNLATAQSLEKE IPAC_SHIDY 197 4 -

Motif 7 width=23
Element Seqn Id St Int Rpt
EQQISQVNNRVASTASDEARESS Q56020 353 133 -
EQQISQVNNRVASTASDEARESS Q56025 353 133 -
EQLISQASSKQAEEASQVSKEAS IPAC_SHIFL 326 107 -
EQLISQASSKQAEEASQVSKEAS IPAC_SHIDY 326 107 -

Motif 8 width=28
Element Seqn Id St Int Rpt
LIQEMLKTMESINQSKASALAAIAGNIR Q56020 381 5 -
LIQEMLKTMESINQSKASALAAIAGNIR Q56025 381 5 -
LIQKLLNIIDSINQSKNSAASQIAGNIR IPAC_SHIFL 354 5 -
LIQKLLNIIDNINQSRSSTASQIAGNIR IPAC_SHIDY 354 5 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
TASQSVSAKDILNSIGISSSKVS Q56020 25 25 -
TASQSVSAKDILNSIGISSSKVS Q56025 25 25 -
TASQSVSAKDILNSIGISSSKVS Q56135 25 25 -
TKPTQTLYTDISTKQTQSSSETQ IPAC_SHIFL 25 25 -
TKPTQTLYTDISTKQTQSSSETQ Q9AJW2 6 6 -
TKSTQILYTDISTKQTQSSSETQ IPAC_SHIDY 25 25 -

Motif 2 width=25
Element Seqn Id St Int Rpt
TLSAPAPGVLTQTPGTITSSLKASI Q56020 54 6 -
TLSAPAPGVLTQTPGTITSFLKASI Q56025 54 6 -
TLSAPAPGVLTQTPGTITSFLKASI Q56135 54 6 -
QIAAHIPLNVGKNPVLTTTLNDDQL IPAC_SHIFL 54 6 -
QIAAHIPLNVGKNPVLTTTLNDDQL Q9AJW2 35 6 -
QIAAHIPLNVGKNPVLTTTLNDDQL IPAC_SHIDY 54 6 -

Motif 3 width=29
Element Seqn Id St Int Rpt
DISGMSSSAVALLAAANTLMLTLNQADSK Q56020 118 39 -
DISGMSSSAVALLAAANTLMLTLNQADSK Q56025 118 39 -
DISGMSSSAVALLAAANTLMLTLNQADSK Q56135 118 39 -
DISSLSSNAVSLIISVAVLLSALRTAETK IPAC_SHIFL 117 38 -
DISSLSSNAVSLIISVAVLLSALRTAETK Q9AJW2 98 38 -
DISSLSSNAVSLIISVAVLLSALRTAETK IPAC_SHIDY 117 38 -

Motif 4 width=20
Element Seqn Id St Int Rpt
LSLVSFDAAKTTASSMMREG Q56020 151 4 -
LSLVSFDAAKTTASSMMREG Q56025 151 4 -
LSLVSFDAAKTTASSMMREG Q56135 151 4 -
LSLIAFDATKSAAENIVRQG IPAC_SHIFL 150 4 -
LSLIAFDATKSAAENIVRQG Q9AJW2 131 4 -
LSLIAFDATKSAAENIVRQG IPAC_SHIDY 150 4 -

Motif 5 width=24
Element Seqn Id St Int Rpt
GMNALSGSISQSALQLGITGVGAK Q56020 170 -1 -
GMNALSGSISQSALQLGITGVGAK Q56025 170 -1 -
GMNALSGSISQSALQLGITGVGAK Q56135 170 -1 -
GLAALSSSITGAVTQVGITGIGAK IPAC_SHIFL 169 -1 -
GLAALSSSITGAVTQVGITGIGAK Q9AJW2 150 -1 -
GLAALSSSITGAVTQVGITGIGAK IPAC_SHIDY 169 -1 -

Motif 6 width=22
Element Seqn Id St Int Rpt
GLQNERGALKHNAAKIDKLTTE Q56020 198 4 -
GLQNERGALKHNAAKIDKLTTE Q56025 198 4 -
GLQNERGALKHNAAKIDKLTTE Q56135 198 4 -
GISDQKGALRKNLATAQSLEKE IPAC_SHIFL 197 4 -
GISDQKGALRKNLATAQSLEKE Q9AJW2 178 4 -
GISDQKGALRKNLATAQSLEKE IPAC_SHIDY 197 4 -

Motif 7 width=23
Element Seqn Id St Int Rpt
EQQISQVNNRVASTASDEARESS Q56020 353 133 -
EQQISQVNNRVASTASDEARESS Q56025 353 133 -
EQQISQVNNRVASTASDEARESS Q56135 353 133 -
EQLISQASSKQAEEASQVSKEAS IPAC_SHIFL 326 107 -
EQLISQASSKQAEEASQVSKEAS Q9AJW2 307 107 -
EQLISQASSKQAEEASQVSKEAS IPAC_SHIDY 326 107 -

Motif 8 width=28
Element Seqn Id St Int Rpt
LIQEMLKTMESINQSKASALAAIAGNIR Q56020 381 5 -
LIQEMLKTMESINQSKASALAAIAGNIR Q56025 381 5 -
LIQEMLKTMESINQSKASALAAIAGNIR Q56135 381 5 -
LIQKLLNIIDSINQSKNSAASQIAGNIR IPAC_SHIFL 354 5 -
LIQKLLNIIDSINQSKNSAASQIAGNIR Q9AJW2 335 5 -
LIQKLLNIIDNINQSRSSTASQIAGNIR IPAC_SHIDY 354 5 -