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PR01593

Identifier
SOPEPROTEIN  [View Relations]  [View Alignment]  
Accession
PR01593
No. of Motifs
9
Creation Date
14-AUG-2001
Title
Salmonella type III secretion SopE effector protein signature
Database References
Literature References
1. HUECK, C.J.
Type III protein secretion systems in bacterial pathogens of animals and 
plants.
MICROBIOL.MOL.BIOL.REV. 379-433 (1998). 
 
2. HARDT, W.D., URLAUB, H. AND GALAN, J.E. 
A substrate of the centrisome 63 type III protein secretion system of 
Salmonella typhimurium is encoded by a cryptic bacteriophage.
PROC.NATL.ACAD.SCI.U.S.A. 95 2574-2579 (1998).
 
3. MUKHERJEE, K., PARASHURAMAN, S., RAJE, M. AND MUKHOPADHYAY, A.
SopE acts as an Rab5-specific nucleotide exchange factor and recruits non-
prenylated Rab5 on Salmonella-containing phagosomes to promote fusion with 
early endosomes.
J.BIOL.CHEM. 276 23607-23615 (2001).

Documentation
Secretion of virulence factors in Gram-negative bacteria involves 
transportation of the protein across two membranes to reach the cell 
exterior [1]. There have been four secretion systems described in 
animal enteropathogens, such as Salmonella and Yersinia, with further 
sequence similarities in plant pathogens like Ralstonia and Erwinia [1]. 
 
The type III secretion system is of great interest, as it is used to 
transport virulence factors from the pathogen directly into the host cell 
[1] and is only triggered when the bacterium comes into close contact with
the host. The protein subunits of the system are very similar to those of 
bacterial flagellar biosynthesis [1]. However, while the latter forms a
ring structure to allow secretion of flagellin and is an integral part of
the flagellum itself [1], type III subunits in the outer membrane
translocate secreted proteins through a channel-like structure.
 
Effector proteins secreted by the type III system do not possess a secretion 
signal, and are considered unique because of this [1]. Salmonella spp. 
secrete an effector protein called SopE that is responsible for stimulating 
the reorganisation of the host cell actin cytoskeleton, and ruffling of the 
cellular membrane [2]. It acts as a guanyl-nucleotide-exchange factor on Rho- 
GTPase proteins such as Cdc42 and Rac. As it is imperative for the bacterium 
to revert the cell back to its "normal" state as quickly as possible, 
another tyrosine phosphatase effector called SptP reverses the actions 
brought about by SopE [2,3]. 
 
Recently, it has been found that SopE and its protein homologue SopE2 can
activate different sets of Rho-GTPases in the host cell [3]. Far from being 
a redundant set of two similar type III effectors, they both act in unison 
to specifically activate different Rho-GTPase signalling cascades in the
host cell during infection [3].
 
SOPEPROTEIN is a 9-element fingerprint that provides a signature for 
the Salmonella type III secretion SopE effector protein family. The 
fingerprint was derived from an initial alignment of 5 sequences: the motifs 
were drawn from conserved regions spanning the full alignment length (~240
amino acids). A single iteration on SPTR39_17f was required to reach 
convergence, no further sequences being identified beyond the starting set.
Summary Information
5 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9555555555
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
O06949        O52623        Q9KIZ2        Q9L8K7        
Q9RPM6
Sequence Titles
O06949      SOPE - Salmonella dublin.                     
O52623 SOPE - Salmonella typhimurium.
Q9KIZ2 SOPE2 - Salmonella typhimurium.
Q9L8K7 INVASION-ASSOCIATED SECRETED PROTEIN SOPE2 - Salmonella typhimurium.
Q9RPM6 SOPE - Salmonella typhi.
Scan History
SPTR39_17f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
FRIQKQETTLLKEKSTEK O52623 11 11 -
FRIQKQETTLLKEKSTEK Q9RPM6 11 11 -
FRIQKQEATPLKEKSTEK O06949 11 11 -
YRIHRSDVEPVKEKTTEK Q9KIZ2 11 11 -
YRIHRSDVEPVKEKTTEK Q9L8K7 11 11 -

Motif 2 width=15
Element Seqn Id St Int Rpt
KSILAVKNHFIELRS O52623 33 4 -
KSILAVKNHFIELRS Q9RPM6 33 4 -
KSILAVKNHFIKLNS O06949 33 4 -
KSITAVRNSFISLST Q9KIZ2 33 4 -
KSITAVRNSFISLST Q9L8K7 33 4 -

Motif 3 width=15
Element Seqn Id St Int Rpt
SERFISHKNTESSAT O52623 50 2 -
SERFISHKNTESSAT Q9RPM6 50 2 -
SERFISHKNTESSAT O06949 50 2 -
SDRFSLHQQTDIPTT Q9KIZ2 50 2 -
SDRFSLHQQTDIPTT Q9L8K7 50 2 -

Motif 4 width=16
Element Seqn Id St Int Rpt
FHRGSASEGRAVLTNK O52623 66 1 -
FHRGSASEGRAVLTNK Q9RPM6 66 1 -
FHRGSASEGRAVLTNK O06949 66 1 -
FHRGNASEGRAVLTSK Q9KIZ2 66 1 -
FHRGNASEGRAVLTSK Q9L8K7 66 1 -

Motif 5 width=19
Element Seqn Id St Int Rpt
FMLQTLNDIDIRGSASKDP O52623 86 4 -
FMLQTLNDIDIRGSASKDP Q9RPM6 86 4 -
FMLQTLHDIDIRGSASKDP O06949 86 4 -
FMLQKLNSLDIKGNASKDP Q9KIZ2 86 4 -
FMLQKLNSLDIKGNASKDP Q9L8K7 86 4 -

Motif 6 width=16
Element Seqn Id St Int Rpt
YSKNKDQCCNLLISKG O52623 119 14 -
YSKNKDQCCNLLISKG Q9RPM6 119 14 -
YSKYKDQYCNLLISKG O06949 119 14 -
YSNNKDQCCKLLISKG Q9KIZ2 119 14 -
YSNNKDHCCKLLISKG Q9L8K7 119 14 -

Motif 7 width=19
Element Seqn Id St Int Rpt
PGTTKNDVFTPSGAGANPF O52623 154 19 -
PGTTKNDVFTPSGAGANPF Q9RPM6 154 19 -
PGATKNDVFSPSGAGANPF O06949 154 19 -
PGEIKNGVFTPGGAGANPF Q9KIZ2 154 19 -
PGEIKNGVFTPGGAGANPF Q9L8K7 154 19 -

Motif 8 width=15
Element Seqn Id St Int Rpt
KYPRMFINQHQQASF O52623 183 10 -
KYPRMFINQHQQASF Q9RPM6 183 10 -
KYPHMFTSQHQKASF O06949 183 10 -
KYPHMFINHNQQVSF Q9KIZ2 183 10 -
KYPHMFINHNQQVSF Q9L8K7 183 10 -

Motif 9 width=15
Element Seqn Id St Int Rpt
PLFNECAMPTPQQFQ O52623 211 13 -
PLFNECAMPTPQQFQ Q9RPM6 211 13 -
PLFNECAMPTPQQFQ O06949 211 13 -
PLFNKGTMPTPQQFQ Q9KIZ2 211 13 -
PLFNKGTMPTPQQFQ Q9L8K7 211 13 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
FRIQKQETTLLKEKSTEK O52623 11 11 -
FRIQKQETTLLKEKSTEK Q9RPM6 11 11 -
FRIQKQEATPLKEKSTEK O06949 11 11 -
YRIHRSDVEPVKEKTTEK Q9KIZ2 11 11 -
YRIHRSDVEPVKEKTTEK Q9L8K7 11 11 -

Motif 2 width=15
Element Seqn Id St Int Rpt
KSILAVKNHFIELRS O52623 33 4 -
KSILAVKNHFIELRS Q9RPM6 33 4 -
KSILAVKNHFIKLNS O06949 33 4 -
KSITAVRNSFISLST Q9KIZ2 33 4 -
KSITAVRNSFISLST Q9L8K7 33 4 -

Motif 3 width=15
Element Seqn Id St Int Rpt
SERFISHKNTESSAT O52623 50 2 -
SERFISHKNTESSAT Q9RPM6 50 2 -
SERFISHKNTESSAT O06949 50 2 -
SDRFSLHQQTDIPTT Q9KIZ2 50 2 -
SDRFSLHQQTDIPTT Q9L8K7 50 2 -

Motif 4 width=16
Element Seqn Id St Int Rpt
FHRGSASEGRAVLTNK O52623 66 1 -
FHRGSASEGRAVLTNK Q9RPM6 66 1 -
FHRGSASEGRAVLTNK O06949 66 1 -
FHRGNASEGRAVLTSK Q9KIZ2 66 1 -
FHRGNASEGRAVLTSK Q9L8K7 66 1 -

Motif 5 width=19
Element Seqn Id St Int Rpt
FMLQTLNDIDIRGSASKDP O52623 86 4 -
FMLQTLNDIDIRGSASKDP Q9RPM6 86 4 -
FMLQTLHDIDIRGSASKDP O06949 86 4 -
FMLQKLNSLDIKGNASKDP Q9KIZ2 86 4 -
FMLQKLNSLDIKGNASKDP Q9L8K7 86 4 -

Motif 6 width=16
Element Seqn Id St Int Rpt
YSKNKDQCCNLLISKG O52623 119 14 -
YSKNKDQCCNLLISKG Q9RPM6 119 14 -
YSKYKDQYCNLLISKG O06949 119 14 -
YSNNKDQCCKLLISKG Q9KIZ2 119 14 -
YSNNKDHCCKLLISKG Q9L8K7 119 14 -

Motif 7 width=19
Element Seqn Id St Int Rpt
PGTTKNDVFTPSGAGANPF O52623 154 19 -
PGTTKNDVFTPSGAGANPF Q9RPM6 154 19 -
PGATKNDVFSPSGAGANPF O06949 154 19 -
PGEIKNGVFTPGGAGANPF Q9KIZ2 154 19 -
PGEIKNGVFTPGGAGANPF Q9L8K7 154 19 -

Motif 8 width=15
Element Seqn Id St Int Rpt
KYPRMFINQHQQASF O52623 183 10 -
KYPRMFINQHQQASF Q9RPM6 183 10 -
KYPHMFTSQHQKASF O06949 183 10 -
KYPHMFINHNQQVSF Q9KIZ2 183 10 -
KYPHMFINHNQQVSF Q9L8K7 183 10 -

Motif 9 width=15
Element Seqn Id St Int Rpt
PLFNECAMPTPQQFQ O52623 211 13 -
PLFNECAMPTPQQFQ Q9RPM6 211 13 -
PLFNECAMPTPQQFQ O06949 211 13 -
PLFNKGTMPTPQQFQ Q9KIZ2 211 13 -
PLFNKGTMPTPQQFQ Q9L8K7 211 13 -