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PR01534

Identifier
VOMERONASL1R  [View Relations]  [View Alignment]  
Accession
PR01534
No. of Motifs
8
Creation Date
17-MAY-2001
Title
Vomeronasal type 1 receptor family signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. KEVERNE, E.B.
The vomeronasal organ.
SCIENCE 286 716-720 (1999).
 
7. PANTAGES, E. AND DULAC, C.
A novel family of candidate pheromone receptors in mammals.
NEURON 28 835-845 (2000).
 
8. JOSEFSSON, L.-G.
Evidence for kinship between diverse G protein coupled receptors.
GENE 239 333-340 (1999).
 
9. GIORGI, D., FRIEDMAN, C., TRASK, B.J. AND ROUQUIER, S.
Characterization of nonfunctional V1R-like pheromone receptor sequences in
human.
GENOME RES. 10 1979-1985 (2000).
 
10. RODRIGUEZ, I., GREER, C.A., MOK, M.Y. AND MOMBAERTS, P.
A putative pheromone receptor gene expressed in human olfactory mucosa.
NAT.GENET. 26 18-19 (2000).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
 
Pheromones have evolved in all animal phyla, to signal sex and dominance
status, and are responsible for stereotypical social and sexual behaviour
among members of the same species. In mammals, these chemical signals are
believed to be detected primarily by the vomeronasal organ (VNO), a
chemosensory organ located at the base of the nasal septum [7]. The VNO is
present in most amphibia, reptiles and non-primate mammals but is absent in
birds, adult catarrhine monkeys and apes [6]. An active role for the human
VNO in the detection of pheromones is disputed; the VNO is clearly present 
in the foetus but appears to be atrophied or absent in adults [6]. Three 
distinct families of putative pheromone receptors have been identified in 
the vomeronasal organ (V1Rs, V2Rs and V3Rs). All are G protein-coupled 
receptors but are only distantly related to the receptors of the main 
olfactory system, highlighting their different role [7].
 
The V1 receptors share between 50 and 90% sequence identity but have little
similarity to other families of G protein-coupled receptors. They appear to
be distantly related to the mammalian T2R bitter taste receptors [7] and the
rhodopsin-like GPCRs [8]. In rat, the family comprises 30-40 genes. These
are expressed in the apical regions of the VNO, in neurons expressing Gi2.
Coupling of the receptors to this protein mediates inositol trisphosphate
signalling [7]. A number of human V1 receptor homologues have also been
found. The majority of these human sequences are pseudogenes [9] but an
apparently functional receptor has been identified that is expressed in the
human olfactory system [10].
 
VOMERONASL1R is an 8-element fingerprint that provides a signature for the
vomeronasal type 1 receptor family. The fingerprint was derived from an
initial alignment of 10 sequences: the motifs were drawn from conserved
sections within the loop and C-terminal regions, focusing on those areas
of the alignment that characterise the vomeronasal type 1 receptors but
distinguish them from the rest of the rhodopsin-like GPCR superfamily -
motif 1 lies in the first cytoplasmic loop; motif 2 spans the first external
loop; motif 3 encodes the second cytoplasmic loop; motif 4 lies in the
second external loop; motifs 5 and 6 span the third cytoplasmic loop; motif
7 encodes the third external loop; and motif 8 resides at the C-terminus.
Two iterations on SPTR39_15f were required to reach convergence, at which 
point a true set comprising 12 sequences was identified.
Summary Information
12 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
81212121212121212
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
Q62850        Q62851        Q62852        Q62853        
Q62854 Q62855 Q62856 Q9WUF1
Q9WUU2 Q9WUU3 Q9Z195 Q9Z196
Sequence Titles
Q62850      PHEROMONE RECEPTOR VN1 - Rattus norvegicus (Rat). 
Q62851 PHEROMONE RECEPTOR VN7 - Rattus norvegicus (Rat).
Q62852 PHEROMONE RECEPTOR VN3 - Rattus norvegicus (Rat).
Q62853 PHEROMONE RECEPTOR VN4 - Rattus norvegicus (Rat).
Q62854 PHEROMONE RECEPTOR VN5 - Rattus norvegicus (Rat).
Q62855 PHEROMONE RECEPTOR VN6 - Rattus norvegicus (Rat).
Q62856 PHEROMONE RECEPTOR VN2 - Rattus norvegicus (Rat).
Q9WUF1 VOMERONASAL RECEPTOR VRI2 - Mus musculus (Mouse).
Q9WUU2 M21 PHEROMONE RECEPTOR - Mus musculus (Mouse).
Q9WUU3 M24 PHEROMONE RECEPTOR - Mus musculus (Mouse).
Q9Z195 PHEROMONE RECEPTOR 1 - Mus musculus (Mouse).
Q9Z196 PHEROMONE RECEPTOR 2 - Mus musculus (Mouse).
Scan History
SPTR39_15f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
ENKPKPIDLYI Q9WUF1 42 42 -
ENRPKPFHLYI Q9WUU3 40 40 -
GHRLRLTDLPI Q9Z196 43 43 -
GQRSRLTDLPI Q62850 43 43 -
GQRSRLTDLPI Q62856 53 53 -
ENKPKPIHLYI Q62853 67 67 -
ENKPKPIHLYI Q9WUU2 42 42 -
GHRSRLSDLPI Q9Z195 44 44 -
GQRSRLTDLPI Q62852 43 43 -
ERRLRITDLII Q62855 42 42 -

Motif 2 width=15
Element Seqn Id St Int Rpt
WNSTTCQSLIYLHRL Q9WUF1 80 27 -
WDSTPCQSLIYLHRL Q9WUU3 78 27 -
WDDIICKFLVYLYRV Q9Z196 81 27 -
WDDIICKSLLYLYRT Q62850 81 27 -
WDDIICKSLLYLYRT Q62856 91 27 -
WDSTSCQSLIYLHRL Q62853 105 27 -
WDSTSCQSLIYLHRL Q9WUU2 80 27 -
WDDIICKFLVYLYRV Q9Z195 82 27 -
WDDIICKFLVYLYRS Q62852 81 27 -
WNDIKCKSLAHLHRL Q62855 80 27 -

Motif 3 width=18
Element Seqn Id St Int Rpt
SSRSSCLTKFKHKSPHHI Q9WUF1 115 20 -
SPRSSCLSKFKHNSPHHI Q9WUU3 113 20 -
SPRSSCLAKLKHKYPHHI Q9Z196 116 20 -
SPRSSCLAKFKHKPSHHI Q62850 116 20 -
SPRSSCLAKYKHKPPHHI Q62856 126 20 -
SSKKSCLTKFKHNSPHHI Q62853 140 20 -
SSKKSRLTKFKHNSPHHI Q9WUU2 115 20 -
SPRSSCLAKFKRKSLHHI Q9Z195 117 20 -
SPRSSCLAKFKHKSPHHV Q62852 116 20 -
SPRSSCLAKFKYKSTQHS Q62855 115 20 -

Motif 4 width=19
Element Seqn Id St Int Rpt
TSEDFMYVTQSCSLLPMSY Q9WUF1 161 28 -
TSNIFMYVTQSCSLLPMSY Q9WUU3 159 28 -
TRNDFLYVTQSCSILPLSY Q9Z196 162 28 -
TTNDFIHVTQWCSILPMSY Q62850 162 28 -
TTNDFIHVSQSCSILPMSY Q62856 172 28 -
TSDNFMYVTKSCSFLPMCY Q62853 186 28 -
TSDNFMYVTKSCSFLPMCY Q9WUU2 161 28 -
TTNDFIYVTQSCSILPLSY Q9Z195 163 28 -
TTNNFMQVTQSCYIIPLSY Q62852 162 28 -
SSRSLIFVTESCIILPMDY Q62855 161 28 -

Motif 5 width=15
Element Seqn Id St Int Rpt
WRHKKQAQHLRSTSL Q9WUF1 212 32 -
WRHKKQAQHLHSTSL Q9WUU3 210 32 -
CRHRKQAQHLQGTSL Q9Z196 213 32 -
CRHRKQTRHLQGTSL Q62850 213 32 -
CRHRKQTRHLQDTSL Q62856 223 32 -
WRHRKQAQHLHSTGL Q62853 237 32 -
WRHRKQAQHLHSTGL Q9WUU2 212 32 -
CRHRKKTQHLQGISL Q9Z195 214 32 -
CRHRNQIQHLQGTNL Q62852 213 32 -
CRHRKQAQHLHRTSL Q62855 212 32 -

Motif 6 width=14
Element Seqn Id St Int Rpt
LSSKASPEQRATRT Q9WUF1 226 -1 -
LSSKASPERRATRT Q9WUU3 224 -1 -
LSPKASAEQRATQT Q9Z196 227 -1 -
LSPKASPEQRATRS Q62850 227 -1 -
LSRKASPEQRATRS Q62856 237 -1 -
LSSKSSPEQRATET Q62853 251 -1 -
LSSKASPEQRATET Q9WUU2 226 -1 -
LSPKTSPKQRATQT Q9Z195 228 -1 -
LSPKASPEQRATQT Q62852 227 -1 -
LSPKASPEQRATRT Q62855 226 -1 -

Motif 7 width=12
Element Seqn Id St Int Rpt
SRLKFKDCSVFY Q9WUF1 260 20 -
SRMKFKDGSMFY Q9WUU3 258 20 -
SRTMFLDDPTSY Q9Z196 261 20 -
SRTMYLNDPISY Q62850 261 20 -
SRTMYLNDPTSY Q62856 271 20 -
SRMKFKDGSTFY Q62853 285 20 -
SRMKFKDGSTFY Q9WUU2 260 20 -
SRTMFLNDPTSY Q9Z195 262 20 -
SRTMYLNDPTSY Q62852 261 20 -
SRLMHNGEPIHH Q62855 260 20 -

Motif 8 width=12
Element Seqn Id St Int Rpt
SPFVFICTEKHI Q9WUF1 285 13 -
SPFVFICTEKHM Q9WUU3 283 13 -
SPFVFMSTEKHI Q9Z196 286 13 -
SPFVFIVTEKHI Q62850 286 13 -
SPFVFMITEKHI Q62856 296 13 -
SSFVFIFTEKRM Q62853 310 13 -
SSFVFIFTEKRM Q9WUU2 285 13 -
SPFVFMSTEKHI Q9Z195 287 13 -
SPFVFMSTGKHI Q62852 286 13 -
SPLLLIVTENRI Q62855 285 13 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
ENKPKPIDLYI Q9WUF1 42 42 -
ENRPKPFHLYI Q9WUU3 40 40 -
KNRSKPIDLYI Q62854 5 5 -
GHRLRLTDLPI Q9Z196 43 43 -
ENRSKPIDLCI Q62851 5 5 -
GQRSRLTDLPI Q62850 43 43 -
GQRSRLTDLPI Q62856 53 53 -
ENKPKPIHLYI Q62853 67 67 -
ENKPKPIHLYI Q9WUU2 42 42 -
GHRSRLSDLPI Q9Z195 44 44 -
GQRSRLTDLPI Q62852 43 43 -
ERRLRITDLII Q62855 42 42 -

Motif 2 width=15
Element Seqn Id St Int Rpt
WNSTTCQSLIYLHRL Q9WUF1 80 27 -
WDSTPCQSLIYLHRL Q9WUU3 78 27 -
WDSTTCQSLIYLDRL Q62854 43 27 -
WDDIICKFLVYLYRV Q9Z196 81 27 -
WDITTCRSLIYFHRL Q62851 43 27 -
WDDIICKSLLYLYRT Q62850 81 27 -
WDDIICKSLLYLYRT Q62856 91 27 -
WDSTSCQSLIYLHRL Q62853 105 27 -
WDSTSCQSLIYLHRL Q9WUU2 80 27 -
WDDIICKFLVYLYRV Q9Z195 82 27 -
WDDIICKFLVYLYRS Q62852 81 27 -
WNDIKCKSLAHLHRL Q62855 80 27 -

Motif 3 width=18
Element Seqn Id St Int Rpt
SSRSSCLTKFKHKSPHHI Q9WUF1 115 20 -
SPRSSCLSKFKHNSPHHI Q9WUU3 113 20 -
SPRSSCLTTFKHKSPHHI Q62854 78 20 -
SPRSSCLAKLKHKYPHHI Q9Z196 116 20 -
SPRSSCLTKFKHKSPHHI Q62851 78 20 -
SPRSSCLAKFKHKPSHHI Q62850 116 20 -
SPRSSCLAKYKHKPPHHI Q62856 126 20 -
SSKKSCLTKFKHNSPHHI Q62853 140 20 -
SSKKSRLTKFKHNSPHHI Q9WUU2 115 20 -
SPRSSCLAKFKRKSLHHI Q9Z195 117 20 -
SPRSSCLAKFKHKSPHHV Q62852 116 20 -
SPRSSCLAKFKYKSTQHS Q62855 115 20 -

Motif 4 width=19
Element Seqn Id St Int Rpt
TSEDFMYVTQSCSLLPMSY Q9WUF1 161 28 -
TSNIFMYVTQSCSLLPMSY Q9WUU3 159 28 -
TSDNFMYVTKSCSFLPMSY Q62854 124 28 -
TRNDFLYVTQSCSILPLSY Q9Z196 162 28 -
TSDHFMYVTQSCSLLPMSY Q62851 124 28 -
TTNDFIHVTQWCSILPMSY Q62850 162 28 -
TTNDFIHVSQSCSILPMSY Q62856 172 28 -
TSDNFMYVTKSCSFLPMCY Q62853 186 28 -
TSDNFMYVTKSCSFLPMCY Q9WUU2 161 28 -
TTNDFIYVTQSCSILPLSY Q9Z195 163 28 -
TTNNFMQVTQSCYIIPLSY Q62852 162 28 -
SSRSLIFVTESCIILPMDY Q62855 161 28 -

Motif 5 width=15
Element Seqn Id St Int Rpt
WRHKKQAQHLRSTSL Q9WUF1 212 32 -
WRHKKQAQHLHSTSL Q9WUU3 210 32 -
WRHKNQARHLHSTSL Q62854 175 32 -
CRHRKQAQHLQGTSL Q9Z196 213 32 -
WRHKKQAQHLHSTRL Q62851 175 32 -
CRHRKQTRHLQGTSL Q62850 213 32 -
CRHRKQTRHLQDTSL Q62856 223 32 -
WRHRKQAQHLHSTGL Q62853 237 32 -
WRHRKQAQHLHSTGL Q9WUU2 212 32 -
CRHRKKTQHLQGISL Q9Z195 214 32 -
CRHRNQIQHLQGTNL Q62852 213 32 -
CRHRKQAQHLHRTSL Q62855 212 32 -

Motif 6 width=14
Element Seqn Id St Int Rpt
LSSKASPEQRATRT Q9WUF1 226 -1 -
LSSKASPERRATRT Q9WUU3 224 -1 -
LSSKVSPEQRATRT Q62854 189 -1 -
LSPKASAEQRATQT Q9Z196 227 -1 -
LSSKASPQQRATRT Q62851 189 -1 -
LSPKASPEQRATRS Q62850 227 -1 -
LSRKASPEQRATRS Q62856 237 -1 -
LSSKSSPEQRATET Q62853 251 -1 -
LSSKASPEQRATET Q9WUU2 226 -1 -
LSPKTSPKQRATQT Q9Z195 228 -1 -
LSPKASPEQRATQT Q62852 227 -1 -
LSPKASPEQRATRT Q62855 226 -1 -

Motif 7 width=12
Element Seqn Id St Int Rpt
SRLKFKDCSVFY Q9WUF1 260 20 -
SRMKFKDGSMFY Q9WUU3 258 20 -
SRMTFKDGSMFY Q62854 223 20 -
SRTMFLDDPTSY Q9Z196 261 20 -
SRTKFKDGSIFY Q62851 223 20 -
SRTMYLNDPISY Q62850 261 20 -
SRTMYLNDPTSY Q62856 271 20 -
SRMKFKDGSTFY Q62853 285 20 -
SRMKFKDGSTFY Q9WUU2 260 20 -
SRTMFLNDPTSY Q9Z195 262 20 -
SRTMYLNDPTSY Q62852 261 20 -
SRLMHNGEPIHH Q62855 260 20 -

Motif 8 width=12
Element Seqn Id St Int Rpt
SPFVFICTEKHI Q9WUF1 285 13 -
SPFVFICTEKHM Q9WUU3 283 13 -
SPFVFICTEKRI Q62854 248 13 -
SPFVFMSTEKHI Q9Z196 286 13 -
SPFVFVFSEKRI Q62851 248 13 -
SPFVFIVTEKHI Q62850 286 13 -
SPFVFMITEKHI Q62856 296 13 -
SSFVFIFTEKRM Q62853 310 13 -
SSFVFIFTEKRM Q9WUU2 285 13 -
SPFVFMSTEKHI Q9Z195 287 13 -
SPFVFMSTGKHI Q62852 286 13 -
SPLLLIVTENRI Q62855 285 13 -