SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01514

Identifier
KV21CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01514
No. of Motifs
7
Creation Date
19-JUN-2001
Title
Kv2.1 voltage-gated K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01491 KVCHANNEL; PR01495 SHABCHANNEL
Literature References
1. MILLER, C.
An overview of the potassium channel family.
GENOME BIOL. 1(4) 1-5 (2000).
 
2. ASHCROFT, F.M.
Voltage-gated K+ channels.
IN ION CHANNELS AND DISEASE, ACADEMIC PRESS, 2000, PP.97-123.
 
3. SANSOM, M.S.
Putting the parts together.
CURR.BIOL. 9(19) R738-R741 (1999).   
 
4. CONLEY, E.C. AND BRAMMAR, W.J.
Shab.
IN THE ION CHANNEL FACTSBOOK, VOLUME IV, ACADEMIC PRESS, 1999, PP.524-558.

Documentation
Potassium ion (K+) channels are a structurally diverse group of proteins
that facilitate the flow of K+ ions across cell membranes. They are
ubiquitous, being present in virtually all cell types. Activation of K+
channels tends to hyperpolarise cells, reducing the membrane's electrical
resistance, dampening nervous activity. In eukaryotic cells, K+ channels
are involved in neural signalling and generation of the cardiac rhythm, and
act as effectors in signal transduction pathways involving G protein-
coupled receptors (GPCRs). In prokaryotic cells, they play a role in the
maintenance of ionic homeostasis [1].
      
Structurally, Kv channels belong to the subfamily of K+ channels whose
subunits contain 6 transmembrane (TM) domains: these are the voltage-
gated K+ (Kv) channels, the KCNQ channels, the EAG-like K+ channels and
3 kinds of Ca2+-activated K+ channels (BK, IK and SK) [2]. All K+
channels share a characteristic sequence feature: a TMxTVGYG motif
that resides between the 2 C-terminal membrane spanning helices, and
forms the K+-selective pore domain [1-2].
     
The Kv family can be divided into 4 subfamilies on the basis of sequence
similarity and function: Shaker (Kv1), Shab (Kv2), Shaw (Kv3) and Shal 
(Kv4). All consist of pore-forming alpha subunits that associate with 
different types of beta subunit. To form a functional K+ channel pore,
4 alpha subunits and 4 beta subunits are required. The alpha subunits
have 6 well-conserved TM domains, a pore loop region and variable length
N- and C-termini. TM domains 5 and 6, and the loop region, form the K+ 
channel pore through which the K+ ions pass [3]. 
    
The Shab voltage-gated delayed rectifier K+ channels (also known as Kv2 
channels) are responsible for much of the delayed rectifier current in 
Drosophila nervous system and muscle. However, in vertebrates, Kv2 channels
have largely undetermined roles in the delayed rectifier currents of the 
heart and skeletal muscle. Kv2 channels can be further divided into 2
subtypes, designated Kv2.1 and Kv2.2 [4].
 
Kv2.1 channels are expressed in the neurons. Essential for their function
is protein phosphorylation dependent on protein kinase A. Three isoforms 
exhibiting temporal patterning during neuronal development have also been
discovered, implying distinct roles for these channels in development[4].  
 
KV21CHANNEL is a 7-element fingerprint that provides a signature for the
Kv2.1 voltage-gated K+ channel. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved regions
spanning the C-terminal third of the alignment, focusing on those sections
that characterise the Kv2.1 channel but distinguish it from other members
of the Shab channel family. Two iterations on SPTR39_15f were required to
reach convergence, at which point a true set comprising 7 sequences was
identified. Two partial matches were also found, both of which are closely-
related members of the Shab family.
Summary Information
   7 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
1 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
77777777
60000000
50000000
41111000
30000000
20100010
1234567
True Positives
CIKA_HUMAN    CIKA_RAT      O18868        Q03717        
Q14193 Q91593 Q9MZ19
True Positive Partials
Codes involving 4 elements
Q9QUU4
Codes involving 2 elements
Q9V736
Sequence Titles
CIKA_HUMAN  VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN KV2.1 (DHK1) - Homo sapiens (Human). 
CIKA_RAT VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN KV2.1 (DRK1) - Rattus norvegicus (Rat).
O18868 DELAYED RECTIFIER POTASSIUM CHANNEL KV2.1 - Sus scrofa (Pig).
Q03717 POTASSIUM CHANNEL PROTEIN - Mus musculus (Mouse).
Q14193 H-DRK1 K(+) CHANNEL (DJ791K14.1) (POTASSIUM VOLTAGE-GATED CHANNEL, SHAB-RELATED SUBFAMILY, MEMBER 1) - Homo sapiens (Human).
Q91593 POTASSIUM CHANNEL ALPHA SUBUNIT KV2.1 - Xenopus laevis (African clawed frog).
Q9MZ19 VOLTAGE-GATED POTASSIUM CHANNEL KV2.1 - Oryctolagus cuniculus (Rabbit).

Q9QUU4 K+ CHANNEL, DRK1 - Rattus sp.

Q9V736 CG12864 PROTEIN - Drosophila melanogaster (Fruit fly).
Scan History
SPTR39_15f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
KNGENMGKKDKV CIKA_HUMAN 463 463 -
KNVENMGQKDKV O18868 467 467 -
KNGESIAKKDKV CIKA_RAT 463 463 -
KTDETSGRKDKV Q91593 467 467 -

Motif 2 width=13
Element Seqn Id St Int Rpt
EQGSPEKARSSSS CIKA_HUMAN 504 29 -
EQGSPEKARSSSS O18868 508 29 -
EQGSPEKARSSSS CIKA_RAT 504 29 -
EQGSPEKTRSGSS Q91593 508 29 -

Motif 3 width=13
Element Seqn Id St Int Rpt
LNVQQLEDMYNKM CIKA_HUMAN 520 3 -
LNVQQLEDMYNKM O18868 524 3 -
LNVQQLEDMYSKM CIKA_RAT 520 3 -
LNVQQLEDIYNKM Q91593 524 3 -

Motif 4 width=12
Element Seqn Id St Int Rpt
KTQSQPILNTKE CIKA_HUMAN 534 1 -
KTQSQPILNTKE O18868 538 1 -
KTQSQPILNTKE CIKA_RAT 534 1 -
KTQSQPILNSKD Q91593 538 1 -

Motif 5 width=12
Element Seqn Id St Int Rpt
TEGVIDMRSMSS CIKA_HUMAN 572 26 -
TEGVIDMRSMSS O18868 576 26 -
TEGVIDMRSMSS CIKA_RAT 572 26 -
REGFIDMRSMSS Q91593 577 27 -

Motif 6 width=12
Element Seqn Id St Int Rpt
YTTASAKTPPRS CIKA_HUMAN 722 138 -
YTTASARTPPRS O18868 726 138 -
YTTASARTPPRS CIKA_RAT 721 137 -
YTTASARTPSKS Q91593 731 142 -

Motif 7 width=15
Element Seqn Id St Int Rpt
YIDADTDDEGQLLYS CIKA_HUMAN 751 17 -
YIDADTDDEGQVLYS O18868 755 17 -
YIDTDTDDEGQLLYS CIKA_RAT 750 17 -
YIDADTDDEGQLLDG Q91593 761 18 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
KNGENMGKKDKV CIKA_HUMAN 463 463 -
KNGENMGKKDKV Q14193 467 467 -
KNVENMGQKDKV O18868 467 467 -
KNGESIAKKDKV CIKA_RAT 463 463 -
KNGENLAKKEKV Q9MZ19 467 467 -
KNGEGVAKKDKV Q03717 467 467 -
KTDETSGRKDKV Q91593 467 467 -

Motif 2 width=13
Element Seqn Id St Int Rpt
EQGSPEKARSSSS CIKA_HUMAN 504 29 -
EQGSPEKARSSSS Q14193 508 29 -
EQGSPEKARSSSS O18868 508 29 -
EQGSPEKARSSSS CIKA_RAT 504 29 -
EQGSPEKARSSSS Q9MZ19 508 29 -
EQGSPEKARSSSS Q03717 508 29 -
EQGSPEKTRSGSS Q91593 508 29 -

Motif 3 width=13
Element Seqn Id St Int Rpt
LNVQQLEDMYNKM CIKA_HUMAN 520 3 -
LNVQQLEDMYNKM Q14193 524 3 -
LNVQQLEDMYNKM O18868 524 3 -
LNVQQLEDMYSKM CIKA_RAT 520 3 -
LNVQQLEDMYNKM Q9MZ19 524 3 -
LNVQQLQDMYSKM Q03717 524 3 -
LNVQQLEDIYNKM Q91593 524 3 -

Motif 4 width=12
Element Seqn Id St Int Rpt
KTQSQPILNTKE CIKA_HUMAN 534 1 -
KTQSQPILNTKE Q14193 538 1 -
KTQSQPILNTKE O18868 538 1 -
KTQSQPILNTKE CIKA_RAT 534 1 -
KTQSQPVLNTKE Q9MZ19 538 1 -
KTQSQPILNTKE Q03717 538 1 -
KTQSQPILNSKD Q91593 538 1 -

Motif 5 width=12
Element Seqn Id St Int Rpt
TEGVIDMRSMSS CIKA_HUMAN 572 26 -
TEGVIDMRSMSS Q14193 576 26 -
TEGVIDMRSMSS O18868 576 26 -
TEGVIDMRSMSS CIKA_RAT 572 26 -
TEGVIDMRSMSS Q9MZ19 576 26 -
TEGVIDMRSMSS Q03717 576 26 -
REGFIDMRSMSS Q91593 577 27 -

Motif 6 width=12
Element Seqn Id St Int Rpt
YTTASAKTPPRS CIKA_HUMAN 722 138 -
YTTASAKTPPRS Q14193 726 138 -
YTTASARTPPRS O18868 726 138 -
YTTASARTPPRS CIKA_RAT 721 137 -
YTTASARTPPRS Q9MZ19 726 138 -
YTTASARTPPRS Q03717 725 137 -
YTTASARTPSKS Q91593 731 142 -

Motif 7 width=15
Element Seqn Id St Int Rpt
YIDADTDDEGQLLYS CIKA_HUMAN 751 17 -
YIDADTDDEGQLLYS Q14193 755 17 -
YIDADTDDEGQVLYS O18868 755 17 -
YIDTDTDDEGQLLYS CIKA_RAT 750 17 -
YIDADTDDEGQLLYS Q9MZ19 755 17 -
YIDTDTDDEGQLLYS Q03717 754 17 -
YIDADTDDEGQLLDG Q91593 761 18 -