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PR01476

Identifier
LTBRECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01476
No. of Motifs
5
Creation Date
13-MAR-2001
Title
Leukotriene B4 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01477 LTB1RECEPTOR; PR01478 LTB2RECEPTOR
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Leukotrienes.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994,
PP.181-187.
 
7. YOKOMIZO, T., IZUMI, T. AND SHIMIZU, T.
Leukotriene B4: metabolism and signal transduction.
ARCH.BIOCHEM.BIOPHYS. 385(2) 231-241 (2001).
 
8. WANG, S., GUSTAFSON, E., PANG, L., QIAO, X., BEHAN, J., MAGUIRE, M.,
BAYNE, M. AND LAZ, T.
A novel hepatointestinal leukotriene B4 receptor.
J.BIOL.CHEM. 275(52) 40686-40694 (2000).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para
-crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of 
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5].
 
Leukotrienes (LT) are potent lipid mediators derived from arachidonic acid
metabolism. They can be divided into two classes, based on the presence or
absence of a cysteinyl group. Leukotriene B4 (LTB4) does not contain such a
group, whereas LTC4, LTD4, LTE4 and LTF4 are cysteinyl leukotrienes [6].
 
LTB4 is one of the most effective chemoattractant mediators known, and is
produced predominantly by neutrophils and macrophages. It is involved in a
number of events, including: stimulation of leukocyte migration from the
bloodstream; activation of neutrophils; inflammatory pain; host defense
against infection; increased interleukin production and transcription [8].
It is found in elevated concentrations in a number of inflammatory and
allergic conditions, such as asthma, psoriasis, rheumatoid arthritis and
inflammatory bowel disease, and has been implicated in the pathogenesis of
these diseases [6,8].
 
Binding sites for LTB4 have been observed in membrane preparations from
leukocytes, macrophages and spleen. Two receptors for LTB4 have since been
cloned (BLT1 and BLT2); both are members of the rhodopsin-like G protein-
coupled receptor superfamily [7].
 
LTBRECEPTOR is a 5-element fingerprint that provides a signature for the
leukotriene B4 receptors. The fingerprint was derived from an initial
alignment of 6 sequences: the motifs were drawn from conserved sections
within loop and C-terminal regions, focusing on those areas of the alignment
that characterise the leukotriene B4 receptors but distinguish them from the
rest of the rhodopsin-like superfamily - motif 1 resides at the C-terminus
of TM domain 2, leading into the first external loop; motif 2 is located in
the first external loop, leading into TM domain 3; motif 3 spans the second
cytoplasmic loop; motif 4 is located in the second external loop, leading 
into TM domain 5; and motif 5 is located at the C-terminus, proximal to TM
domain 7. Two iterations on SPTR39_15f were required to reach convergence,
at which point a true set comprising 7 sequences was identified.
Summary Information
7 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
577777
400000
300000
200000
12345
True Positives
O88855        P2Y7_HUMAN    Q9JJL9        Q9NPC1        
Q9NPE5 Q9R0Q2 Q9WTK1
Sequence Titles
O88855      LEUKOTRIENE B4 RECEPTOR - Mus musculus (Mouse). 
P2Y7_HUMAN P2Y PURINOCEPTOR 7 (P2Y7) (LEUKOTRIENE B4 RECEPTOR) (CHEMOATTRACTANT RECEPTOR-LIKE 1) - Homo sapiens (Human).
Q9JJL9 LEUKOTRIENE B4 RECEPTOR, BLT2 - Mus musculus (Mouse).
Q9NPC1 LEUKOTRIENE B4 RECEPTOR 2 (SEVEN TRANSMEMBRANE RECEPTOR BLTR2) - Homo sapiens (Human).
Q9NPE5 LEUKOTRIENE B4 RECEPTOR BLT2 (LTB4 RECEPTOR JULF2) - Homo sapiens (Human).
Q9R0Q2 LEUKOTRIENE B4 RECEPTOR - Rattus norvegicus (Rat).
Q9WTK1 LTB4 RECEPTOR - Cavia porcellus (Guinea pig).
Scan History
SPTR39_15f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
LLTAPFFLHFLARGTW Q9R0Q2 70 70 -
LLTAPFFLHFLARGTW O88855 70 70 -
LLLTPLFVAFLTRQAW Q9NPC1 103 103 -
LLLTPLFVAFLTRQAW Q9NPE5 72 72 -
LLTAPFFLHFLTWHTW Q9WTK1 70 70 -
LLLTPLFVAFLSQEAW Q9JJL9 72 72 -

Motif 2 width=13
Element Seqn Id St Int Rpt
GCRLCHYVCGVSM Q9R0Q2 91 5 -
GCRLCHYVCGISM O88855 91 5 -
GCKAVYYVCALSM Q9NPC1 124 5 -
GCKAVYYVCALSM Q9NPE5 93 5 -
GCRLCHYICGVSM Q9WTK1 91 5 -
GCKAVYYVCALSM Q9JJL9 93 5 -

Motif 3 width=15
Element Seqn Id St Int Rpt
RPFVSQKVRTKAFAR Q9R0Q2 123 19 -
RPFMSQKVRTKAFAR O88855 123 19 -
RPFLAPRLRSPALAR Q9NPC1 156 19 -
RPFLAPRLRSPALAR Q9NPE5 125 19 -
SPFLSQKVRTKTAAR Q9WTK1 123 19 -
RPFLAPRLRSPALAR Q9JJL9 125 19 -

Motif 4 width=13
Element Seqn Id St Int Rpt
PSDGHKVFHLLFE Q9R0Q2 174 36 -
PNKEHKVFHLLFE O88855 174 36 -
PSPVHAAAHLSLE Q9NPC1 204 33 -
PSPVHAAAHLSLE Q9NPE5 173 33 -
PSDRHKAFHLLFE Q9WTK1 175 37 -
PSPVHAAAHLSLE Q9JJL9 173 33 -

Motif 5 width=21
Element Seqn Id St Int Rpt
GGLLRSAGVGFVVKLLEGTGS Q9R0Q2 290 103 -
GGLLRSAGVGFVVKLLEGTGS O88855 290 103 -
GDLLPRAGPRFLTRLFEGSGE Q9NPC1 324 107 -
GDLLPRAGPRFLTRLFEGSGE Q9NPE5 293 107 -
GGLLRSAGVGFVAKLLEATGS Q9WTK1 289 101 -
GDLLPRAGPRFLTRLFEGSGE Q9JJL9 293 107 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
LLTAPFFLHFLARGTW Q9R0Q2 70 70 -
LLTAPFFLHFLAQGTW P2Y7_HUMAN 68 68 -
LLTAPFFLHFLARGTW O88855 70 70 -
LLLTPLFVAFLTRQAW Q9NPC1 103 103 -
LLLTPLFVAFLTRQAW Q9NPE5 72 72 -
LLTAPFFLHFLTWHTW Q9WTK1 70 70 -
LLLTPLFVAFLSQEAW Q9JJL9 72 72 -

Motif 2 width=13
Element Seqn Id St Int Rpt
GCRLCHYVCGVSM Q9R0Q2 91 5 -
GCRLCHYVCGVSM P2Y7_HUMAN 89 5 -
GCRLCHYVCGISM O88855 91 5 -
GCKAVYYVCALSM Q9NPC1 124 5 -
GCKAVYYVCALSM Q9NPE5 93 5 -
GCRLCHYICGVSM Q9WTK1 91 5 -
GCKAVYYVCALSM Q9JJL9 93 5 -

Motif 3 width=15
Element Seqn Id St Int Rpt
RPFVSQKVRTKAFAR Q9R0Q2 123 19 -
RPFVSQKLRTKAMAR P2Y7_HUMAN 121 19 -
RPFMSQKVRTKAFAR O88855 123 19 -
RPFLAPRLRSPALAR Q9NPC1 156 19 -
RPFLAPRLRSPALAR Q9NPE5 125 19 -
SPFLSQKVRTKTAAR Q9WTK1 123 19 -
RPFLAPRLRSPALAR Q9JJL9 125 19 -

Motif 4 width=13
Element Seqn Id St Int Rpt
PSDGHKVFHLLFE Q9R0Q2 174 36 -
PSEGHRAFHLIFE P2Y7_HUMAN 173 37 -
PNKEHKVFHLLFE O88855 174 36 -
PSPVHAAAHLSLE Q9NPC1 204 33 -
PSPVHAAAHLSLE Q9NPE5 173 33 -
PSDRHKAFHLLFE Q9WTK1 175 37 -
PSPVHAAAHLSLE Q9JJL9 173 33 -

Motif 5 width=21
Element Seqn Id St Int Rpt
GGLLRSAGVGFVVKLLEGTGS Q9R0Q2 290 103 -
GGLLRSAGVGFVAKLLEGTGS P2Y7_HUMAN 290 104 -
GGLLRSAGVGFVVKLLEGTGS O88855 290 103 -
GDLLPRAGPRFLTRLFEGSGE Q9NPC1 324 107 -
GDLLPRAGPRFLTRLFEGSGE Q9NPE5 293 107 -
GGLLRSAGVGFVAKLLEATGS Q9WTK1 289 101 -
GDLLPRAGPRFLTRLFEGSGE Q9JJL9 293 107 -