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PR01457

Identifier
LENSMEMPROT  [View Relations]  [View Alignment]  
Accession
PR01457
No. of Motifs
5
Creation Date
17-NOV-2000
Title
Lens fibre membrane intrinsic protein (LMIP) signature
Database References
PRINTS; PR01453 EPMEMFAMILY
MIM; 154045
Literature References
1. TAYLOR, V., WELCHER, A.A., PROGRAM, A.E. AND SUTER, U.
Epithelial membrane protein-1, peripheral myelin protein 22, and lens 
membrane protein 20 define a novel gene family.
J.BIOL.CHEM. 270 28824-28833 (1995).
 
2. LOBSIGER, C., MAGYAR, J., TAYLOR, V., WULF, P., WELCHER, A., PROGRAM A.E.
AND SUTER, U.
Identification and characterization of a cDNA and the structural gene 
encoding the mouse epithelial membrane protein-1.
GENOMICS 36 379-387 (1996).
 
3. LOUIS, C., HUR, K., GALVAN, A., TENBROEK, E., JARVIS, I., ECCLESTON, E.
AND HOWARD, J.
Identification of an 18,000-dalton protein in mammalian lens fiber cell
proteins.
J.BIOL.CHEM. 264 19967-19973 (1989).
 
4. TENBROEK, E., ARNESON, M., JARVIS, L. AND LOUIS, C.
The distribution of the fiber cell intrinsic membrane proteins MP20 and 
connexin46 in the bovine lens.
J.CELL SCI. 103 245-257 (1992).
 
5. GALVAN, A., LAMPE, P., HUR, K., HOWARD, J., ECCLESTON, E., ARNESON, M.
AND LOUIS, C.
Structural organization of the lens fiber cell plasma membrane protein MP18.
J.BIOL.CHEM. 264 19974-19978 (1989).

Documentation
The epithelial membrane proteins (EMP-1, -2 and -3), peripheral myelin 
protein 22 (PMP22), and lens fibre membrane intrinsic protein (LMIP)
comprise a protein family on the basis of sequence and structural 
similarities [1]. Each family member is a small hydrophobic membrane
glycoprotein, ~160-170 amino acids in length, and shares a common predicted
transmembrane (TM) topology of 4 TM domains, with intracellular N- and 
C-termini [2]. 
 
LMIP, previously termed MP17, MP18, MP19 and MP20, is expressed at extremely
high levels in the mammalian eye lens, where it is localised to the thicker
(16-17nm) cell membrane junctions [3]. Although its function has not been 
elucidated, it has been shown to co-localise with connexin-46, suggesting 
that it may be involved in the organisation of junctional plaques [4]. A
role for LMIP in signal transduction has also been proposed, as it is
phosphorylated by cAMP-dependent kinase and binds calmodulin [5].
 
LENSMEMPROT is a 5-element fingerprint that provides a signature for lens
fibre membrane intrinsic protein (LMIP). The fingerprint was derived from
an initial alignment of 3 sequences: the motifs were drawn from conserved
TM and loop regions, focusing on those sections that characterise lens 
fibre membrane protein LIMP and distinguish it from other family members -
motifs 1 and 2 reside within the first extracellular loop; motif 3 lies in
the intracellular loop; motif 4 resides in TM domain 3; and motif 5 lies 
in the second extracellular loop. A single iteration on SPTR39_14f was 
required to reach convergence, no further sequences being identified 
beyond the starting set. 
Summary Information
3 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
533333
400000
300000
200000
12345
True Positives
LMIP_BOVIN    LMIP_HUMAN    LMIP_RAT      
Sequence Titles
LMIP_BOVIN  LENS FIBER MEMBRANE INTRINSIC PROTEIN (MP18) (MP19) (MP21) (MP23) - Bos taurus (Bovine). 
LMIP_HUMAN LENS FIBER MEMBRANE INTRINSIC PROTEIN (MP18) (MP19) (MP20) - Homo sapiens (Human).
LMIP_RAT LENS FIBER MEMBRANE INTRINSIC PROTEIN (MP17) (MP18) (MP19) (MP20) - Rattus norvegicus (Rat).
Scan History
SPTR39_14f 1  200  NSINGLE    
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
MQYRLSGSFAHQGL LMIP_RAT 29 29 -
MQYRLSGAFAHQGL LMIP_BOVIN 29 29 -
MQYRLSGSFAHQGL LMIP_HUMAN 29 29 -

Motif 2 width=17
Element Seqn Id St Int Rpt
CLGNKCFLQTESIAYWN LMIP_RAT 46 3 -
CLGTKCYLQTESIAYWN LMIP_BOVIN 46 3 -
CLGNKCYLQTDSIAYWN LMIP_HUMAN 46 3 -

Motif 3 width=12
Element Seqn Id St Int Rpt
TFTRLSRPFSAG LMIP_RAT 91 28 -
TFTRLSRPFSAG LMIP_BOVIN 91 28 -
TFSRISRPFSAG LMIP_HUMAN 91 28 -

Motif 4 width=14
Element Seqn Id St Int Rpt
MFFASTLFVLLALA LMIP_RAT 104 1 -
MFFASTFFVLLALA LMIP_BOVIN 104 1 -
MFFSSSLFVVLALA LMIP_HUMAN 104 1 -

Motif 5 width=15
Element Seqn Id St Int Rpt
TGVTVSFLGRRFGDW LMIP_RAT 120 2 -
TGVTVSFLGRRFGDW LMIP_BOVIN 120 2 -
TGVTVSFLGRRFGDW LMIP_HUMAN 120 2 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
MQYRLSGSFAHQGL LMIP_RAT 29 29 -
MQYRLSGAFAHQGL LMIP_BOVIN 29 29 -
MQYRLSGSFAHQGL LMIP_HUMAN 29 29 -

Motif 2 width=17
Element Seqn Id St Int Rpt
CLGNKCFLQTESIAYWN LMIP_RAT 46 3 -
CLGTKCYLQTESIAYWN LMIP_BOVIN 46 3 -
CLGNKCYLQTDSIAYWN LMIP_HUMAN 46 3 -

Motif 3 width=12
Element Seqn Id St Int Rpt
TFTRLSRPFSAG LMIP_RAT 91 28 -
TFTRLSRPFSAG LMIP_BOVIN 91 28 -
TFSRISRPFSAG LMIP_HUMAN 91 28 -

Motif 4 width=14
Element Seqn Id St Int Rpt
MFFASTLFVLLALA LMIP_RAT 104 1 -
MFFASTFFVLLALA LMIP_BOVIN 104 1 -
MFFSSSLFVVLALA LMIP_HUMAN 104 1 -

Motif 5 width=15
Element Seqn Id St Int Rpt
TGVTVSFLGRRFGDW LMIP_RAT 120 2 -
TGVTVSFLGRRFGDW LMIP_BOVIN 120 2 -
TGVTVSFLGRRFGDW LMIP_HUMAN 120 2 -