SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01453

Identifier
EPMEMFAMILY  [View Relations]  [View Alignment]  
Accession
PR01453
No. of Motifs
4
Creation Date
23-NOV-2000
Title
Epithelial membrane protein (EMP/PMP22/LMIP) family signature
Database References
PRINTS; PR01454 EPMEMPROT1; PR01455 EPMEMPROT2; PR01456 EPMEMPROT3
PRINTS; PR01457 LENSMEMPROT; PR01458 PMYELIN22
PROSITE; PS01221 PMP22_1; PS01222 PMP22_2
BLOCKS; IPB000729
PFAM; PF00822 PMP22
PRODOM; PD005274
INTERPRO; IPR000729
Literature References
1. TAYLOR, V., WELCHER, A.A., PROGRAM, A.E. AND SUTER, U.
Epithelial membrane protein-1, peripheral myelin protein 22, and lens 
membrane protein 20 define a novel gene family.
J.BIOL.CHEM. 270 28824-28833 (1995).
 
2. LOBSIGER, C., MAGYAR, J., TAYLOR, V., WULF, P., WELCHER, A., PROGRAM A.E.
AND SUTER, U.
Identification and characterization of a cDNA and the structural gene 
encoding the mouse epithelial membrane protein-1.
GENOMICS 36 379-387 (1996).
 
3. ZOIDL, G., BLASS-KAMPMANN, S., D'URSO, D., SCHMALENBACH, C. AND MULLER, H.
Retroviral-mediated gene transfer of the peripheral myelin protein PMP22 in
Schwann cells: modulation of cell growth.
EMBO J. 14 1122-1128 (1995).
 
4. BEN-PORATH, I. AND BENVENISTY, N.
Characterization of a tumor-associated gene, a member of a novel family
of genes encoding membrane glycoproteins.
GENE 183 69-75 (1996).
 
5. LOUIS, C., HUR, K., GALVAN, A., TENBROEK, E., JARVIS, I., ECCLESTON, E.
AND HOWARD, J.
Identification of an 18,000-dalton protein in mammalian lens fiber cell
proteins.
J.BIOL.CHEM. 264 19967-19973 (1989).

Documentation
The epithelial membrane proteins (EMP-1, -2 and -3), peripheral myelin 
protein 22 (PMP22), and lens fiber membrane intrinsic protein (LMIP)
comprise a protein family on the basis of sequence and structural 
similarities [1]. Each family member is a small hydrophobic membrane
glycoprotein, ~160-170 amino acids in length, and shares a common predicted
transmembrane (TM) topology of 4 TM domains, with intracellular N- and 
C-termini [2]. 
 
Roles for the EMP/PMP22/LMIP family members in cell structure and growth
and have been proposed [1]. PMP22 is a structural component of compact
myelin in the peripheral nervous system. It has also been implicated in the
control of cell proliferation. PMP22 expression levels are up-regulated in
cultured cells undergoing growth arrest [1], and modulation of PMP22 levels
in cultured Schwann cells exerts a profound effect on the length of the G1
phase of the cell cycle [3]. In contrast, EMP-1, which is co-expressed with
PMP22 in a range of tissues, displays an inverse pattern of regulation.
EMP-1 expression levels are high in proliferating cells and can be
down-regulated by inducing growth arrest [4]. It has therefore been
proposed that these proteins may perform reciprocal roles in the control of
cell quiescence and proliferation. Although the function of LMIP has not
been elucidated, its extremely high expression level in the mammalian eye
lens suggests it may perform a tissue specific role [5].
 
EPMEMFAMILY is a 4-element fingerprint that provides a signature for the 
epithelial membrane protein (EMP/PMP22/LMIP) family. The fingerprint was
derived from an initial alignment of 15 sequences: the motifs were drawn
from conserved loop and TM regions, focusing on those sections that
characterise the EMP/PMP22/LMIP family - motif 1 lies in TM domain 2; motif 
2 spans part of the intracellular loop and part of TM domain 3; motif 3 
resides within TM domain 3; and motif 4 lies in TM domain 4. Two iterations
on SPTR39_14f were required to reach convergence, at which point a true set
comprising 16 sequences was identified. A single partial match was also 
found, Q9TQZ3, a preliminary partial bovine sequence that is highly similar
to PMP22.
Summary Information
  16 codes involving  4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
416161616
30000
20110
1234
True Positives
EMP1_HUMAN    EMP1_MOUSE    EMP1_RABIT    EMP1_RAT      
EMP2_HUMAN EMP3_HUMAN EMP3_MOUSE LMIP_BOVIN
LMIP_HUMAN LMIP_RAT O88333 O88662
PM22_HUMAN PM22_MOUSE PM22_RAT Q9QYW5
True Positive Partials
Codes involving 2 elements
Q9TQZ3
Sequence Titles
EMP1_HUMAN  EPITHELIAL MEMBRANE PROTEIN-1 (EMP-1) (TUMOR-ASSOCIATED MEMBRANE PROTEIN) (CL-20) (B4B PROTEIN) - Homo sapiens (Human). 
EMP1_MOUSE EPITHELIAL MEMBRANE PROTEIN-1 (EMP-1) (TUMOR-ASSOCIATED MEMBRANE PROTEIN) - Mus musculus (Mouse).
EMP1_RABIT EPITHELIAL MEMBRANE PROTEIN-1 (EMP-1) (TUMOR-ASSOCIATED MEMBRANE PROTEIN) (SQUAMOUS CELL-SPECIFIC PROTEIN CL-20) - Oryctolagus cuniculus (Rabbit).
EMP1_RAT EPITHELIAL MEMBRANE PROTEIN-1 (EMP-1) (TUMOR-ASSOCIATED MEMBRANE PROTEIN) - Rattus norvegicus (Rat).
EMP2_HUMAN EPITHELIAL MEMBRANE PROTEIN-2 (EMP-2) (XMP PROTEIN) - Homo sapiens (Human).
EMP3_HUMAN EPITHELIAL MEMBRANE PROTEIN-3 (EMP-3) (YMP PROTEIN) (HEMATOPOIETIC NEURAL MEMBRANE PROTEIN) (HNMP-1) - Homo sapiens (Human).
EMP3_MOUSE EPITHELIAL MEMBRANE PROTEIN-3 (EMP-3) (HEMATOPOIETIC NEURAL MEMBRANE PROTEIN) (HNMP-1) - Mus musculus (Mouse).
LMIP_BOVIN LENS FIBER MEMBRANE INTRINSIC PROTEIN (MP18) (MP19) (MP21) (MP23) - Bos taurus (Bovine).
LMIP_HUMAN LENS FIBER MEMBRANE INTRINSIC PROTEIN (MP18) (MP19) (MP20) - Homo sapiens (Human).
LMIP_RAT LENS FIBER MEMBRANE INTRINSIC PROTEIN (MP17) (MP18) (MP19) (MP20) - Rattus norvegicus (Rat).
O88333 YMP - Mus musculus (Mouse).
O88662 XMP - Mus musculus (Mouse).
PM22_HUMAN PERIPHERAL MYELIN PROTEIN 22 (PMP-22) - Homo sapiens (Human).
PM22_MOUSE PERIPHERAL MYELIN PROTEIN 22 (PMP-22) (GROWTH-ARREST-SPECIFIC PROTEIN 3) (GAS3) - Mus musculus (Mouse).
PM22_RAT PERIPHERAL MYELIN PROTEIN 22 (PMP-22) (CD25 PROTEIN) (SR13 MYELIN PROTEIN) - Rattus norvegicus (Rat).
Q9QYW5 EMP3 HOMOLOG - Rattus norvegicus (Rat).

Q9TQZ3 PAS POSITIVE GLYCOPROTEIN, PASII - Bos taurus (Bovine).
Scan History
SPTR39_14f 2  300  NSINGLE    
Initial Motifs
Motif 1  width=8
Element Seqn Id St Int Rpt
AFMILSII EMP1_RABIT 66 66 -
ATMILSII PM22_HUMAN 67 67 -
ATMILSVI PM22_MOUSE 67 67 -
ATMILSVI PM22_RAT 67 67 -
ATMILSTI O88662 71 71 -
ATMILSTI EMP2_HUMAN 66 66 -
AFMILSII EMP1_HUMAN 66 66 -
AFMILSII EMP1_RAT 66 66 -
AFMILSII EMP1_MOUSE 66 66 -
ALMVLSLI EMP3_MOUSE 68 68 -
ALMVLSLI Q9QYW5 68 68 -
ALMVLSLI O88333 68 68 -
VLMVLSLI EMP3_HUMAN 68 68 -
AFMILSAL LMIP_RAT 66 66 -
AFMILSAL LMIP_HUMAN 66 66 -
AFMILSSL LMIP_BOVIN 66 66 -

Motif 2 width=9
Element Seqn Id St Int Rpt
KGNRFFLSG EMP1_RABIT 91 17 -
KGGRFYITG PM22_HUMAN 92 17 -
KGGRFYITG PM22_MOUSE 92 17 -
KGGRFYITG PM22_RAT 92 17 -
QGERFVLTS O88662 96 17 -
QGERFVLTS EMP2_HUMAN 91 17 -
KGNRFFLSG EMP1_HUMAN 91 17 -
KGNRFFLSG EMP1_RAT 91 17 -
KGNRFFLSG EMP1_MOUSE 91 17 -
RGGLFYATG EMP3_MOUSE 93 17 -
RGGLFYATG Q9QYW5 93 17 -
RGGIFYATG O88333 93 17 -
RGGLFYATG EMP3_HUMAN 93 17 -
RLSRPFSAG LMIP_RAT 94 20 -
RISRPFSAG LMIP_HUMAN 94 20 -
RLSRPFSAG LMIP_BOVIN 94 20 -

Motif 3 width=10
Element Seqn Id St Int Rpt
LCVLIGASIY EMP1_RABIT 107 7 -
LCVMSAAAIY PM22_HUMAN 108 7 -
LCVMSAAAIY PM22_MOUSE 108 7 -
LCVMSAAAIY PM22_RAT 108 7 -
LCVMIGASIY O88662 112 7 -
LCVMIAASIY EMP2_HUMAN 107 7 -
LCILVGVSIY EMP1_HUMAN 107 7 -
LCILIGVSIY EMP1_RAT 107 7 -
LCILVGVSIY EMP1_MOUSE 107 7 -
AAVFSGALIY EMP3_MOUSE 109 7 -
AAVFSGALIY Q9QYW5 109 7 -
AAVFSGALIY O88333 109 7 -
VAVFTGALIY EMP3_HUMAN 109 7 -
LFVLLALAIY LMIP_RAT 110 7 -
LFVVLALAIY LMIP_HUMAN 110 7 -
FFVLLALAIY LMIP_BOVIN 110 7 -

Motif 4 width=8
Element Seqn Id St Int Rpt
YSFILAWI EMP1_RABIT 135 18 -
FAYILAWV PM22_HUMAN 134 16 -
FAYILAWV PM22_MOUSE 134 16 -
FAYILAWV PM22_RAT 134 16 -
YSFILAWV O88662 146 24 -
YSYILAWV EMP2_HUMAN 141 24 -
YSYILGWI EMP1_HUMAN 132 15 -
YCFILTWI EMP1_RAT 135 18 -
YCFILTWI EMP1_MOUSE 135 18 -
YCFALAWV EMP3_MOUSE 137 18 -
YCFALAWV Q9QYW5 137 18 -
YCFALAWV O88333 137 18 -
YCFALAWV EMP3_HUMAN 137 18 -
WSYILGWV LMIP_RAT 138 18 -
WSYILGWV LMIP_HUMAN 138 18 -
WSYILGWV LMIP_BOVIN 138 18 -
Final Motifs
Motif 1  width=8
Element Seqn Id St Int Rpt
AFMILSII EMP1_RABIT 66 66 -
ATMILSII PM22_HUMAN 67 67 -
ATMILSVI PM22_MOUSE 67 67 -
ATMILSVI PM22_RAT 67 67 -
ATMILSTI O88662 71 71 -
ATMILSTI EMP2_HUMAN 66 66 -
AFMILSII EMP1_HUMAN 66 66 -
AFMILSII EMP1_RAT 66 66 -
AFMILSII EMP1_MOUSE 66 66 -
ALMVLSLI EMP3_MOUSE 68 68 -
ALMVLSLI Q9QYW5 68 68 -
ALMVLSLI O88333 68 68 -
VLMVLSLI EMP3_HUMAN 68 68 -
AFMILSAL LMIP_RAT 66 66 -
AFMILSAL LMIP_HUMAN 66 66 -
AFMILSSL LMIP_BOVIN 66 66 -

Motif 2 width=9
Element Seqn Id St Int Rpt
KGNRFFLSG EMP1_RABIT 91 17 -
KGGRFYITG PM22_HUMAN 92 17 -
KGGRFYITG PM22_MOUSE 92 17 -
KGGRFYITG PM22_RAT 92 17 -
QGERFVLTS O88662 96 17 -
QGERFVLTS EMP2_HUMAN 91 17 -
KGNRFFLSG EMP1_HUMAN 91 17 -
KGNRFFLSG EMP1_RAT 91 17 -
KGNRFFLSG EMP1_MOUSE 91 17 -
RGGLFYATG EMP3_MOUSE 93 17 -
RGGLFYATG Q9QYW5 93 17 -
RGGIFYATG O88333 93 17 -
RGGLFYATG EMP3_HUMAN 93 17 -
RLSRPFSAG LMIP_RAT 94 20 -
RISRPFSAG LMIP_HUMAN 94 20 -
RLSRPFSAG LMIP_BOVIN 94 20 -

Motif 3 width=10
Element Seqn Id St Int Rpt
LCVLIGASIY EMP1_RABIT 107 7 -
LCVMSAAAIY PM22_HUMAN 108 7 -
LCVMSAAAIY PM22_MOUSE 108 7 -
LCVMSAAAIY PM22_RAT 108 7 -
LCVMIGASIY O88662 112 7 -
LCVMIAASIY EMP2_HUMAN 107 7 -
LCILVGVSIY EMP1_HUMAN 107 7 -
LCILIGVSIY EMP1_RAT 107 7 -
LCILVGVSIY EMP1_MOUSE 107 7 -
AAVFSGALIY EMP3_MOUSE 109 7 -
AAVFSGALIY Q9QYW5 109 7 -
AAVFSGALIY O88333 109 7 -
VAVFTGALIY EMP3_HUMAN 109 7 -
LFVLLALAIY LMIP_RAT 110 7 -
LFVVLALAIY LMIP_HUMAN 110 7 -
FFVLLALAIY LMIP_BOVIN 110 7 -

Motif 4 width=8
Element Seqn Id St Int Rpt
YSFILAWI EMP1_RABIT 135 18 -
FAYILAWV PM22_HUMAN 134 16 -
FAYILAWV PM22_MOUSE 134 16 -
FAYILAWV PM22_RAT 134 16 -
YSFILAWV O88662 146 24 -
YSYILAWV EMP2_HUMAN 141 24 -
YSYILGWI EMP1_HUMAN 132 15 -
YCFILTWI EMP1_RAT 135 18 -
YCFILTWI EMP1_MOUSE 135 18 -
YCFALAWV EMP3_MOUSE 137 18 -
YCFALAWV Q9QYW5 137 18 -
YCFALAWV O88333 137 18 -
YCFALAWV EMP3_HUMAN 137 18 -
WSYILGWV LMIP_RAT 138 18 -
WSYILGWV LMIP_HUMAN 138 18 -
WSYILGWV LMIP_BOVIN 138 18 -