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PR01427

Identifier
TGFBETA4  [View Relations]  [View Alignment]  
Accession
PR01427
No. of Motifs
14
Creation Date
01-NOV-2000
Title
Transforming growth factor beta 4 precursor signature
Database References
PRINTS; PR01423 TGFBETA
MIM; 601877
Literature References
1. KOTHAPALLI, R., BUYUKSAL, I., WU, S.-Q., CHEGINI, N. AND TABIBZADEH, S. 
Detection of ebaf, a novel human gene of the 
transforming growth factor beta superfamily association of gene expression 
with endometrial bleeding. 
J.CLIN.INVEST. 99 2342-2350 (1997).
 
2. MENO, C., SAIJOH, Y., FUJII, H., IKEDA, M., YOKOYAMA, T., YOKOYAMA, M.,
TOYODA, Y. AND HAMADA, H.
Left-right asymmetric expression of the TGF beta-family member lefty in 
mouse embryos.
NATURE 381 151-155 (1996). 

Documentation
Abnormal endometrial bleeding is a common manifestation of gynaecological
diseases. A human gene, termed endometrial bleeding associated factor (ebaf),
which is expressed strongly in endometrium, has been shown to be associated
with abnormal endometrial bleeding [1]. The predicted protein product of 
ebaf shares similarity with members of TGF-beta superfamily. Thus ebaf is a
novel member of the TGF-beta superfamily and an endometrial tissue factor
whose expression is associated with normal menstrual and abnormal 
endometrial bleeding [1]. 
 
Examples of lateral asymmetry are often found in vertebrates (e.g., the 
heart being on the left side), but the molecular mechanisms governing 
establishment of left-right (L-R) handedness are unknown [2]. It is now 
thought that the gene lefty, a member of the transforming growth factor-beta
family, may encode a morphogen for L-R determination [2]. Lefty protein,
which contains the cysteine-knot motif characteristic of this superfamily,
is expressed in the left half of gastrulating mouse embryos. This asymmetric
expression is transient and occurs just before the first sign of lateral
asymmetry appears [2]. Thus lefty may be involved in establishing L-R 
asymmetry in the organ systems of mammals [2]. 
 
TGFBETA4 is a 14-element fingerprint that provides a signature for the 
transforming growth factor beta 4 precursor proteins. The fingerprint was
derived from an initial alignment of 2 sequences: the motifs were drawn from
conserved regions spanning virtually the full alignment length, focusing on
those sections that characterise TGF-beta 4 proteins but distinguish them
from the rest of the TGF-beta superfamily. Two iterations on SPTR39_14f were
required to reach convergence, at which point a true set comprising 4 
sequences was identified. Several partial matches were also found (Q9PVN4, 
Q9W6I7, Q9PW55, Q9W6I6), all antivin or Lefty proteins that match 2-4 motifs.
Summary Information
   4 codes involving 14 elements
0 codes involving 13 elements
0 codes involving 12 elements
0 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
1 codes involving 4 elements
1 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
1444444444444444
1300000000000000
1200000000000000
1100000000000000
1000000000000000
900000000000000
800000000000000
700000000000000
600000000000000
500000000000000
401000001001100
300001001001000
200200002000000
1234567891011121314
True Positives
O75610        O75611        TGF4_HUMAN    TGF4_MOUSE    
True Positive Partials
Codes involving 4 elements
Q9PVN4
Codes involving 3 elements
Q9W6I7
Codes involving 2 elements
Q9PW55 Q9W6I6
Sequence Titles
O75610      SIGNALING MOLECULE LEFTY-B - Homo sapiens (Human). 
O75611 SIGNALING MOLECULE LEFTY-A - Homo sapiens (Human).
TGF4_HUMAN TRANSFORMING GROWTH FACTOR BETA 4 PRECURSOR (TGF-BETA 4) (ENDOMETRIAL BLEEDING-ASSOCIATED FACTOR) - Homo sapiens (Human).
TGF4_MOUSE TRANSFORMING GROWTH FACTOR BETA 4 PRECURSOR (TGF-BETA 4) (LEFTY PROTEIN) (STRA3 PROTEIN) - Mus musculus (Mouse).

Q9PVN4 LEFTY - Gallus gallus (Chicken).

Q9W6I7 SIGNALING MOLECULE LEFTY2 - Brachydanio rerio (Zebrafish) (Zebra danio).

Q9PW55 ANTIVIN - Brachydanio rerio (Zebrafish) (Zebra danio).
Q9W6I6 SIGNALING MOLECULE LEFTY1 - Brachydanio rerio (Zebrafish) (Zebra danio).
Scan History
SPTR39_14f 2  50   NSINGLE    
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
LWLCWALWVLPLAGPGAALTEEQ TGF4_HUMAN 4 4 -
LWLCWALWALSLVSLREALTGEQ TGF4_MOUSE 4 4 -

Motif 2 width=22
Element Seqn Id St Int Rpt
QLLASLLRQLQLSEVPVLDRAD TGF4_HUMAN 26 -1 -
QILGSLLQQLQLDQPPVLDKAD TGF4_MOUSE 26 -1 -

Motif 3 width=17
Element Seqn Id St Int Rpt
EKLVIPAHVRAQYVVLL TGF4_HUMAN 49 1 -
EGMVIPSHVRTQYVALL TGF4_MOUSE 49 1 -

Motif 4 width=19
Element Seqn Id St Int Rpt
RSRGKRFSQSFREVAGRFL TGF4_HUMAN 71 5 -
RSRGKRFSQNLREVAGRFL TGF4_MOUSE 72 6 -

Motif 5 width=16
Element Seqn Id St Int Rpt
HLLVFGMEQRLPPNSE TGF4_HUMAN 96 6 -
HLLVFGMEQRLPPNSE TGF4_MOUSE 97 6 -

Motif 6 width=21
Element Seqn Id St Int Rpt
ELVQAVLRLFQEPVPQGALHR TGF4_HUMAN 111 -1 -
ELVQAVLRLFQEPVPRTALRR TGF4_MOUSE 112 -1 -

Motif 7 width=19
Element Seqn Id St Int Rpt
RHGRLSPAAPKARVTVEWL TGF4_HUMAN 131 -1 -
RQKRLSPHSARARVTIEWL TGF4_MOUSE 132 -1 -

Motif 8 width=22
Element Seqn Id St Int Rpt
RDDGSNRTSLIDSRLVSVHESG TGF4_HUMAN 151 1 -
RDDGSNRTALIDSRLVSIHESG TGF4_MOUSE 153 2 -

Motif 9 width=24
Element Seqn Id St Int Rpt
WQQLSRPPEPLLVQVSVQREHLGP TGF4_HUMAN 185 12 -
WQQLSRPRQPLLLQVSVQREHLGP TGF4_MOUSE 187 12 -

Motif 10 width=16
Element Seqn Id St Int Rpt
HKLVRFASQGAPAGLG TGF4_HUMAN 214 5 -
HKLVRFAAQGTPDGKG TGF4_MOUSE 216 5 -

Motif 11 width=26
Element Seqn Id St Int Rpt
QLELHTLDLRDYGAQGDCDPEAPMTE TGF4_HUMAN 232 2 -
QLELHTLDLKDYGAQGNCDPEAPVTE TGF4_MOUSE 236 4 -

Motif 12 width=22
Element Seqn Id St Int Rpt
RQEMYIDLQGMKWAKNWVLEPP TGF4_HUMAN 263 5 -
RQEMYLDLQGMKWAENWILEPP TGF4_MOUSE 267 5 -

Motif 13 width=21
Element Seqn Id St Int Rpt
QPPEALAFNWPFLGPRQCIAS TGF4_HUMAN 297 12 -
QLPESLTSRWPFLGPRQCVAS TGF4_MOUSE 301 12 -

Motif 14 width=20
Element Seqn Id St Int Rpt
PMIVSIKEGGRTRPQVVSLP TGF4_HUMAN 323 5 -
PMIVSVKEGGRTRPQVVSLP TGF4_MOUSE 327 5 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
LWLCWALWVLPLAGPGAALTEEQ O75611 4 4 -
LWLCWALWVLPLASPGAALTGEQ O75610 4 4 -
LWLCWALWVLPLAGPGAALTEEQ TGF4_HUMAN 4 4 -
LWLCWALWALSLVSLREALTGEQ TGF4_MOUSE 4 4 -

Motif 2 width=22
Element Seqn Id St Int Rpt
QLLGSLLRQLQLSEVPVLDRAD O75611 26 -1 -
QLLGSLLRQLQLKEVPTLDRAD O75610 26 -1 -
QLLASLLRQLQLSEVPVLDRAD TGF4_HUMAN 26 -1 -
QILGSLLQQLQLDQPPVLDKAD TGF4_MOUSE 26 -1 -

Motif 3 width=17
Element Seqn Id St Int Rpt
EKLVIPAHVRAQYVVLL O75611 49 1 -
EELVIPTHVRAQYVALL O75610 49 1 -
EKLVIPAHVRAQYVVLL TGF4_HUMAN 49 1 -
EGMVIPSHVRTQYVALL TGF4_MOUSE 49 1 -

Motif 4 width=19
Element Seqn Id St Int Rpt
RSRGKRFSQSFREVAGRFL O75611 72 6 -
RSRGKRFSQSFREVAGRFL O75610 72 6 -
RSRGKRFSQSFREVAGRFL TGF4_HUMAN 71 5 -
RSRGKRFSQNLREVAGRFL TGF4_MOUSE 72 6 -

Motif 5 width=16
Element Seqn Id St Int Rpt
HLLVFGMEQRLPPNSE O75611 97 6 -
HLLVFGMEQRLPPNSE O75610 97 6 -
HLLVFGMEQRLPPNSE TGF4_HUMAN 96 6 -
HLLVFGMEQRLPPNSE TGF4_MOUSE 97 6 -

Motif 6 width=21
Element Seqn Id St Int Rpt
ELVQAVLRLFQEPVPKAALHR O75611 112 -1 -
ELVQAVLRLFQEPVPKAALHR O75610 112 -1 -
ELVQAVLRLFQEPVPQGALHR TGF4_HUMAN 111 -1 -
ELVQAVLRLFQEPVPRTALRR TGF4_MOUSE 112 -1 -

Motif 7 width=19
Element Seqn Id St Int Rpt
RHGRLSPRSAQARVTVEWL O75611 132 -1 -
RHGRLSPRSARARVTVEWL O75610 132 -1 -
RHGRLSPAAPKARVTVEWL TGF4_HUMAN 131 -1 -
RQKRLSPHSARARVTIEWL TGF4_MOUSE 132 -1 -

Motif 8 width=22
Element Seqn Id St Int Rpt
RDDGSNRTSLIDSRLVSVHESG O75611 153 2 -
RDDGSNRTSLIDSRLVSVHESG O75610 153 2 -
RDDGSNRTSLIDSRLVSVHESG TGF4_HUMAN 151 1 -
RDDGSNRTALIDSRLVSIHESG TGF4_MOUSE 153 2 -

Motif 9 width=24
Element Seqn Id St Int Rpt
WQQLSRPRQPLLLQVSVQREHLGP O75611 187 12 -
WQQLSRPRQPLLLQVSVQREHLGP O75610 187 12 -
WQQLSRPPEPLLVQVSVQREHLGP TGF4_HUMAN 185 12 -
WQQLSRPRQPLLLQVSVQREHLGP TGF4_MOUSE 187 12 -

Motif 10 width=16
Element Seqn Id St Int Rpt
HKLVRFASQGAPAGLG O75611 216 5 -
HKLVRFASQGAPAGLG O75610 216 5 -
HKLVRFASQGAPAGLG TGF4_HUMAN 214 5 -
HKLVRFAAQGTPDGKG TGF4_MOUSE 216 5 -

Motif 11 width=26
Element Seqn Id St Int Rpt
QLELHTLDLRDYGAQGDCDPEAPMTE O75611 234 2 -
QLELHTLDLGDYGAQGDCDPEAPMTE O75610 234 2 -
QLELHTLDLRDYGAQGDCDPEAPMTE TGF4_HUMAN 232 2 -
QLELHTLDLKDYGAQGNCDPEAPVTE TGF4_MOUSE 236 4 -

Motif 12 width=22
Element Seqn Id St Int Rpt
RQEMYIDLQGMKWAKNWVLEPP O75611 265 5 -
RQEMYIDLQGMKWAENWVLEPP O75610 265 5 -
RQEMYIDLQGMKWAKNWVLEPP TGF4_HUMAN 263 5 -
RQEMYLDLQGMKWAENWILEPP TGF4_MOUSE 267 5 -

Motif 13 width=21
Element Seqn Id St Int Rpt
QPPEALAFNWPFLGPRQCIAS O75611 299 12 -
QPPEALAFKWPFLGPRQCIAS O75610 299 12 -
QPPEALAFNWPFLGPRQCIAS TGF4_HUMAN 297 12 -
QLPESLTSRWPFLGPRQCVAS TGF4_MOUSE 301 12 -

Motif 14 width=20
Element Seqn Id St Int Rpt
PMIVSIKEGGRTRPQVVSLP O75611 325 5 -
PMIVSIKEGGRTRPQVVSLP O75610 325 5 -
PMIVSIKEGGRTRPQVVSLP TGF4_HUMAN 323 5 -
PMIVSVKEGGRTRPQVVSLP TGF4_MOUSE 327 5 -