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PR01408

Identifier
MACSCAVRCPTR  [View Relations]  [View Alignment]  
Accession
PR01408
No. of Motifs
8
Creation Date
27-MAR-2000
Title
Macrophage scavenger receptor signature
Database References
PRINTS; PR00258 SPERACTRCPTR
Literature References
1. DANGOTT, L.J., JORDAN, J.E., BELLET, R.A. AND GARBERS, D.L.
Cloning of the messenger RNA for the protein that cross-links to
egg peptide speract.
PROC.NATL.ACAD.SCI.U.S.A. 86 2128-2132 (1989).
 
2. FREEMAN, M., ASHKENAS, J., REES, D.J., KINGSLEY, D.M., COPELAND, N.G.,
JENKINS, N.A. AND KRIEGER, M.
An ancient, highly conserved family of cysteine-rich protein domains
revealed by cloning type-I and type-II murine macrophage scavenger
receptors.
PROC.NATL.ACAD.SCI.U.S.A. 87 8810-8814 (1990).
 
3. MATSUMOTO, A., NAITO, M., ITAKURA, H., IKEMOTO, S., ASAOKA, H.,
HAYAKAWA, I., KANAMORI, H., ABURATANI, H., TAKAKU, F., SUZUKI, H.
Human macrophage scavenger receptors: primary structure, expression, and
localization in atherosclerotic lesions.
PROC.NATL.ACAD.SCI.U.S.A. 87 9133-9137 (1990).
 
4. KODAMA, T., FREEMAN, M., ROHRER, L., ZABRECKY, J., MATSUDAIRA, P.
AND KRIEGER, M.
Type I macrophage scavenger receptor contains alpha-helical and collagen-
like coiled coils.
NATURE 343 531-535 (1990). 

Documentation
The egg peptide speract receptor is a transmembrane glycoprotein of about
500 amino acids [1]. Topologically, it comprises a large extracellular
domain of about 450 residues, followed by a transmembrane domain and a
short cytoplasmic region of about 12 amino acids. The extracellular
domain contains 4 repeats of a well-conserved region, which spans 115
amino acids and contains 6 conserved cysteines. A similar domain is also
found towards the C-terminus of macrophage scavenger receptor type I [2],
a membrane glycoprotein implicated in the pathologic deposition of
cholesterol in arterial walls during artherogenesis, and in the CD5
glycoprotein, which acts as a receptor in regulating T-cell proliferation.
 
The type I and type II human scavenger receptors are similar to their  
bovine, lapine and murine counterparts. They consist of 6 domains:
cytoplasmic (I); membrane-spanning (II); spacer (III); alpha-helical coiled-
coil (IV); collagen-like (V); and a type-specific C-terminal (VI) [3]. 
Immunohistochemical studies have indicated the presence of scavenger
receptors in the macrophages of lipid-rich atherosclerotic lesions,
suggesting the involvement of these receptors in atherogenesis [3]. 
 
The macrophage scavenger receptor is trimeric and has unusual ligand-binding
properties [4]. The trimeric structure of the bovine type I scavenger 
receptor contains 3 extracellular C-terminal cysteine-rich domains connected
to the transmembrane domain by a long fibrous stalk. The stalk structure,
which consists of an alpha-helical coiled coil and a collagen-like triple
helix, has not previously been observed in an integral membrane protein [4]. 
 
MACSCAVRCPTR is an 8-element fingerprint that provides a signature for
macrophage scavenger receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved regions 
spanning virtually the full alignment length, focusing on those sections
that characterise the macrophage scavenger receptors but distuinguish them
from the speract receptor family - motif 1 lies in the cytoplasmic domain; 
motif 2 includes the transmembrane domain; and motifs 3-8 span the alpha-
helical coiled-coil domain. Two iterations on SPTR37_10f were required to
reach convergence, at which point a true set comprising 5 sequences was
identified. 
Summary Information
5 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
855555555
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
MSRE_BOVIN    MSRE_HUMAN    MSRE_MOUSE    MSRE_RABIT    
O60505
Sequence Titles
MSRE_BOVIN  MACROPHAGE SCAVENGER RECEPTOR TYPES I AND II (MACROPHAGE ACETYLATED LDL RECEPTOR I AND II) - BOS TAURUS (BOVINE). 
MSRE_HUMAN MACROPHAGE SCAVENGER RECEPTOR TYPES I AND II (MACROPHAGE ACETYLATED LDL RECEPTOR I AND II) - HOMO SAPIENS (HUMAN).
MSRE_MOUSE MACROPHAGE SCAVENGER RECEPTOR TYPES I AND II (MACROPHAGE ACETYLATED LDL RECEPTOR I AND II) - MUS MUSCULUS (MOUSE).
MSRE_RABIT MACROPHAGE SCAVENGER RECEPTOR TYPES I AND II (MACROPHAGE ACETYLATED LDL RECEPTOR I AND II) - ORYCTOLAGUS CUNICULUS (RABBIT).
O60505 MACROPHAGE SCAVENGER RECEPTOR TYPE III - HOMO SAPIENS (HUMAN).
Scan History
SPTR37_10f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
ESVKFDARSVTALLPPHPKN MSRE_BOVIN 19 19 -
ESVKFDARSMTASLPHSTKN MSRE_MOUSE 24 24 -
ESVKFDARSNTALLPPNPKN MSRE_RABIT 19 19 -
ESVKFDARSMTALLPPNPKN MSRE_HUMAN 19 19 -

Motif 2 width=26
Element Seqn Id St Int Rpt
YKTALITLYLIVFVVLVPIIGIVAAQ MSRE_BOVIN 49 10 -
FKAALIALYLLVFAVLIPVVGIVTAQ MSRE_MOUSE 54 10 -
FKAALIALYLLVFAVLIPIIAIMAAQ MSRE_RABIT 49 10 -
FKAALIALYLLVFAVLIPLIGIVAAQ MSRE_HUMAN 49 10 -

Motif 3 width=17
Element Seqn Id St Int Rpt
MEKRIQHILDMEANLMD MSRE_HUMAN 121 46 -
MEKRIQYISDTEENLVD MSRE_RABIT 121 46 -
MEERIQSISNSKADLID MSRE_MOUSE 125 45 -
MESRIQYLSDNEANLLD MSRE_BOVIN 120 45 -

Motif 4 width=21
Element Seqn Id St Int Rpt
DQRFNDVLFQLNSLLSSIQEH MSRE_BOVIN 148 11 -
DQRFNDILLQLSTLFSSVQGH MSRE_HUMAN 149 11 -
DQRFADVLLQLSTLVPTVQGH MSRE_RABIT 149 11 -
DQRLNDILLQLNSLILSVQEH MSRE_MOUSE 153 11 -

Motif 5 width=19
Element Seqn Id St Int Rpt
NAIDEISKSLISLNTTLLD MSRE_HUMAN 171 1 -
NAVDEITRSLISLNTTLLD MSRE_RABIT 171 1 -
NSLDAISKSLQSLNMTLLD MSRE_MOUSE 175 1 -
NIIGDISKSLVGLNTTVLD MSRE_BOVIN 170 1 -

Motif 6 width=25
Element Seqn Id St Int Rpt
QEEISKLEERVYNVSAEIMAMKEEQ MSRE_HUMAN 209 19 -
QEEISKLKERVHNASAEIMSMKEEQ MSRE_RABIT 209 19 -
QEDISKLEERVYKVSAEVQSVKEEQ MSRE_MOUSE 213 19 -
QEEMRKLEERIYNASAEIKSLDEKQ MSRE_BOVIN 208 19 -

Motif 7 width=17
Element Seqn Id St Int Rpt
EQEIKGEVKVLNNITND MSRE_HUMAN 237 3 -
EQEIKGEMKLLNNITND MSRE_BOVIN 236 3 -
EQEVKQEVRVLNNITND MSRE_MOUSE 241 3 -
EQEIKREVKVLNNITND MSRE_RABIT 237 3 -

Motif 8 width=20
Element Seqn Id St Int Rpt
LRLKDWEHSQTLKNITLLQG MSRE_BOVIN 253 0 -
LRLKDWEHSQTLKNITFIQG MSRE_MOUSE 258 0 -
LRLKDWEHSQTLRNITLIQG MSRE_RABIT 254 0 -
LRLKDWEHSQTLRNITLIQG MSRE_HUMAN 254 0 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
ESVKFDARSVTALLPPHPKN MSRE_BOVIN 19 19 -
ESVKFDARSMTASLPHSTKN MSRE_MOUSE 24 24 -
ESVKFDARSNTALLPPNPKN MSRE_RABIT 19 19 -
ESVKFDARSMTALLPPNPKN O60505 19 19 -
ESVKFDARSMTALLPPNPKN MSRE_HUMAN 19 19 -

Motif 2 width=26
Element Seqn Id St Int Rpt
YKTALITLYLIVFVVLVPIIGIVAAQ MSRE_BOVIN 49 10 -
FKAALIALYLLVFAVLIPVVGIVTAQ MSRE_MOUSE 54 10 -
FKAALIALYLLVFAVLIPIIAIMAAQ MSRE_RABIT 49 10 -
FKAALIALYLLVFAVLIPLIGIVAAQ O60505 49 10 -
FKAALIALYLLVFAVLIPLIGIVAAQ MSRE_HUMAN 49 10 -

Motif 3 width=17
Element Seqn Id St Int Rpt
MEKRIQHILDMEANLMD MSRE_HUMAN 121 46 -
MEKRIQHILDMEANLMD O60505 121 46 -
MEKRIQYISDTEENLVD MSRE_RABIT 121 46 -
MEERIQSISNSKADLID MSRE_MOUSE 125 45 -
MESRIQYLSDNEANLLD MSRE_BOVIN 120 45 -

Motif 4 width=21
Element Seqn Id St Int Rpt
DQRFNDVLFQLNSLLSSIQEH MSRE_BOVIN 148 11 -
DQRFNDILLQLSTLFSSVQGH MSRE_HUMAN 149 11 -
DQRFNDILLQLSTLFSSVQGH O60505 149 11 -
DQRFADVLLQLSTLVPTVQGH MSRE_RABIT 149 11 -
DQRLNDILLQLNSLILSVQEH MSRE_MOUSE 153 11 -

Motif 5 width=19
Element Seqn Id St Int Rpt
NAIDEISKSLISLNTTLLD MSRE_HUMAN 171 1 -
NAIDEISKSLISLNTTLLD O60505 171 1 -
NAVDEITRSLISLNTTLLD MSRE_RABIT 171 1 -
NSLDAISKSLQSLNMTLLD MSRE_MOUSE 175 1 -
NIIGDISKSLVGLNTTVLD MSRE_BOVIN 170 1 -

Motif 6 width=25
Element Seqn Id St Int Rpt
QEEISKLEERVYNVSAEIMAMKEEQ MSRE_HUMAN 209 19 -
QEEISKLEERVYNVSAEIMAMKEEQ O60505 209 19 -
QEEISKLKERVHNASAEIMSMKEEQ MSRE_RABIT 209 19 -
QEDISKLEERVYKVSAEVQSVKEEQ MSRE_MOUSE 213 19 -
QEEMRKLEERIYNASAEIKSLDEKQ MSRE_BOVIN 208 19 -

Motif 7 width=17
Element Seqn Id St Int Rpt
EQEIKGEVKVLNNITND MSRE_HUMAN 237 3 -
EQEIKGEMKLLNNITND MSRE_BOVIN 236 3 -
EQEVKQEVRVLNNITND MSRE_MOUSE 241 3 -
EQEIKREVKVLNNITND MSRE_RABIT 237 3 -
EQEIKGEVKVLNNITND O60505 237 3 -

Motif 8 width=20
Element Seqn Id St Int Rpt
LRLKDWEHSQTLKNITLLQG MSRE_BOVIN 253 0 -
LRLKDWEHSQTLKNITFIQG MSRE_MOUSE 258 0 -
LRLKDWEHSQTLRNITLIQG MSRE_RABIT 254 0 -
LRLKDWEHSQTLRNITLIQG O60505 254 0 -
LRLKDWEHSQTLRNITLIQG MSRE_HUMAN 254 0 -