SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01388

Identifier
CDTOXINB  [View Relations]  [View Alignment]  
Accession
PR01388
No. of Motifs
6
Creation Date
07-JUN-2000
Title
Cytolethal distending toxin B signature
Database References
Literature References
1. PICKETT, C.L., COTTLE, D.L., PESCI, E.C. AND BIKAH, G.
Cloning, sequencing, and expression of the Escherichia coli cytolethal
distending toxin genes.
INFECT.IMMUN. 62 1046-1051 (1994).
 
2. EYIGOR, I., DAWSON, K.A., LANGLOIS, B.E. AND PICKETT, C.L.
Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter 
coli isolates; detection and analysis by PCR.
J.CLIN.MICROBIOL. 37 1646-1650 (1999).
 
3. DE RYCKE, J., SERT, V., COMAYRAS, C. AND TASCA, C.
Sequence of lethal events in HeLa cells exposed to the G2 blocking 
cytolethal distending toxin.
EUR.J.CELL BIOL. 29 192-201 (2000).

Documentation
Escherichia coli, Haemophilus spp. and Campylobacter spp. all produce 
a toxin that is seen to cause distension in certain cell lines [1,2],
which eventually disintegrate and die. This novel toxin, termed cytolethal 
distending toxin (cdt), has three subunits: A, B, and C. Their sizes are 
approx. 27.7, 29.5 and 19.9kDa respectively [1], and they appear to be 
entirely novel [2]. 
 
Further research on the complete toxin has revealed that it blocks the cell
cycle at stage G2, through inactivation of the cyclin-dependent kinase Cdk1
[3], and without induction of DNA breaks. This leads to multipolar abortive
mitosis and micronucleation, associated with centrosomal amplification [3].
The roles of each subunit are unclear, but it is believed that they have
separate roles in pathogenicity.
 
CDTOXINB is a 6-element fingerprint that provides a signature for cytolethal
distending toxin B proteins. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved regions
spanning the full alignment length (~270 amino acids). Two iterations on
SPTR37_10f were required to reach convergence, at which point a true set
comprising 7 sequences was identified. 
Summary Information
7 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6777777
5000000
4000000
3000000
2000000
123456
True Positives
O06523        O32586        O87121        Q46101        
Q46669 Q47089 Q9ZAB7
Sequence Titles
O06523      CYTOLETHAL DISTENDING TOXIN PROTEIN B - HAEMOPHILUS DUCREYI. 
O32586 CYTOLETHAL DISTENDING TOXIN-IIIB - ESCHERICHIA COLI.
O87121 CYTOLETHAL DISTENDING TOXIN B - ACTINOBACILLUS SP., AND ACTINOBACILLUS ACTINOMYCETEMCOMITANS (HAEMOPHILUS ACTINOMYCETEMCOMITANS).
Q46101 CDTB - CAMPYLOBACTER JEJUNI.
Q46669 CDTB - ESCHERICHIA COLI.
Q47089 CDTB - ESCHERICHIA COLI.
Q9ZAB7 CYTOLETHAL DISTENDING TOXIN PROTEIN B - ACTINOBACILLUS ACTINOMYCETEMCOMITANS (HAEMOPHILUS ACTINOMYCETEMCOMITANS).
Scan History
SPTR37_10f 2  25   NSINGLE    
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
LSDFKVATWNLQGSSAVNESKW Q9ZAB7 24 24 -
LTDFRVATWNLQGASATTESKW Q46669 20 20 -

Motif 2 width=21
Element Seqn Id St Int Rpt
QEAGSLPSSAVRTSRVIQHGG Q9ZAB7 65 19 -
QEAGSPPSTAVDTGTLIPSPG Q46669 61 19 -

Motif 3 width=19
Element Seqn Id St Int Rpt
SRPNMVYIYYSRLDVGANR Q9ZAB7 99 13 -
SRPQQVYIYFSAVDALGGR Q46669 95 13 -

Motif 4 width=20
Element Seqn Id St Int Rpt
RPAVGIRIGTDVFFTVHALA Q9ZAB7 144 26 -
RPLLGIRIGNDAFFTAHAIA Q46669 138 24 -

Motif 5 width=16
Element Seqn Id St Int Rpt
SWMVVGDFNRAPVNLE Q9ZAB7 193 29 -
NWMILGDFNREPADLE Q46669 186 28 -

Motif 6 width=17
Element Seqn Id St Int Rpt
LMLNQLRSQITSDHFPV Q9ZAB7 261 52 -
IVYGARRTQISSDHFPV Q46669 248 46 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
LSDFKVATWNLQGSSAVNESKW O06523 24 24 -
LSDFKVATWNLQGSSAVNESKW O87121 24 24 -
LSDFKVATWNLQGSSAVNESKW Q9ZAB7 24 24 -
LTDFRVATWNLQGASATTESKW Q46669 20 20 -
LTDFRVATWNLQGASATTESKW O32586 20 20 -
LSDFKVATWNLQGSNAPTENKW Q47089 19 19 -
LENFNVGTWNLQGSSAATESKW Q46101 18 18 -

Motif 2 width=21
Element Seqn Id St Int Rpt
QEAGSLPSSAVRTSRVIQHGG O06523 65 19 -
QEAGSLPSSAVRTSRVIQHGG O87121 65 19 -
QEAGSLPSSAVRTSRVIQHGG Q9ZAB7 65 19 -
QEAGSPPSTAVDTGTLIPSPG Q46669 61 19 -
QEAGSPPSTAVDTGRVIPSPG O32586 61 19 -
QEAGAVPASATLTEREFSTPG Q47089 60 19 -
QEAGTLPRTATPTGRHVQQGG Q46101 59 19 -

Motif 3 width=19
Element Seqn Id St Int Rpt
SRPNMVYIYYSRLDVGANR O06523 99 13 -
SRPNMVYIYYSRLDVGANR O87121 99 13 -
SRPNMVYIYYSRLDVGANR Q9ZAB7 99 13 -
SRPQQVYIYFSAVDALGGR Q46669 95 13 -
SRPQQVYIYFSAVDAFGGR O32586 95 13 -
SRPQELFIYFSRVDAFANR Q47089 94 13 -
SRPDRVFIYYSRVDVGANR Q46101 93 13 -

Motif 4 width=20
Element Seqn Id St Int Rpt
RPAVGIRIGTDVFFTVHALA O06523 144 26 -
RPAVGIRIGTDVFFTVHALA O87121 144 26 -
RPAVGIRIGTDVFFTVHALA Q9ZAB7 144 26 -
RPLLGIRIGNDAFFTAHAIA Q46669 138 24 -
RPLLGIRIGNDAFFTAHAIA O32586 138 24 -
RPIIGIRIGNDVFFSTHALA Q47089 137 24 -
RPIIGIRNGNDAFFNIHALA Q46101 136 24 -

Motif 5 width=16
Element Seqn Id St Int Rpt
SWMVVGDFNRAPANLE O06523 193 29 -
SWMVVGDFNRAPVNLE O87121 193 29 -
SWMVVGDFNRAPVNLE Q9ZAB7 193 29 -
NWMILGDFNREPADLE Q46669 186 28 -
NWMILGDFNREPDDLE O32586 186 28 -
NWMIAGDFNRSPATLF Q47089 185 28 -
NWMIAGDFNRDPSTIT Q46101 179 23 -

Motif 6 width=17
Element Seqn Id St Int Rpt
LMLNQLRSQITSDHFPV O06523 261 52 -
LMLNQLRSQITSDHFPV O87121 261 52 -
LMLNQLRSQITSDHFPV Q9ZAB7 261 52 -
IVYGARRTQISSDHFPV Q46669 248 46 -
IVYGARRTQMSSDHYPV O32586 248 46 -
LLFGLLRGQIASDHFPV Q47089 247 46 -
LMLASLRSHIVSDHFPV Q46101 244 49 -