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PR01331

Identifier
KIR61CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01331
No. of Motifs
9
Creation Date
12-MAY-2000
Title
Kir6.1 inward rectifier K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01320 KIRCHANNEL
PRODOM; PD019998; PD024037
Literature References
1. MINOR, D.L., JR., MASSELLING, S.J., JAN, Y.N. AND JAN, L.Y.
Transmembrane structure of an inwardly rectifying potassium channel.
CELL 96 879-891 (1999).
 
2. DOUPNIK, C.A., DAVIDSON, N. AND LESTER, H.A.
The inward rectifier potassium channel family.
CURR.OPIN.NEUROBIOL. 5 268-277 (1995).
 
3. REIMANN, F. AND ASHCROFT, F.M.
Inwardly rectifying potassium channels.
CURR.OPIN.CELL BIOL. 11 503-508 (1999).
 
4. RUSS, U., HAMBROCK, A., ARTUNC, F., LOFFLER-WALTZ, C., HORIO, Y.,
KURACHI, Y. AND QUAST, Q.
Coexpression with the inward rectifier K(+) channel Kir6.1 increases the
affinity of the vascular sulfonylurea receptor SUR2B for glibenclamide. 
MOL.PHARMACOL. 56 955-961 (1999).

Documentation
Potassium channels are found in virtually all cell types. Their pore-
forming subunits fall into three structural families, i.e. those possessing
six, four and two transmembrane (TM) domains. The six-TM domain K+ channels
can be further subdivided into six families: the voltage-gated K+ channels
(Kv), the KCNQ channels, the eag-like K+ channels, and three Ca2+-activated
K+ channels. Inwardly-rectifying K+ channels (Kir) are the principal class
of two-TM domain K+ channels, and the recently-discovered two-pore domain
K+ channels, make up a family of four-TM domain K+ channels.
 
Inwardly rectifying potassium channels (Kir) are responsible for regulating
diverse processes including: cellular excitability, vascular tone, heart
rate, renal salt flow, and insulin release [1]. To date, around twenty
members of this superfamily have been cloned, which can be grouped into six
families by sequence similarity, and these are designated Kir1.x-7.x [2,3].
 
Cloned Kir channel cDNAs encode proteins of between ~370-500 residues,
containing two predicted TM domains, with the characteristic K+ channel
pore-forming domain located between them. Both N- and C-termini are thought
to be cytoplasmic, and the N-terminus lacks a signal sequence. It is thought
that four Kir subunits assemble to form a tetrameric channel complex, which
may be hetero- or homomeric [1].
 
Kir6.x inward rectifier K+ channel family subunits associate with
sulphonylurea receptors (SURs) to form ATP-sensitive K+ (KATP) channels.
These are octameric complexes containing equal numbers of Kir and SUR
subunits. The resulting channels are regulated by cytosolic nucleotides,
such as ATP, and thus link cell metabolism to electrical activity, and K+
fluxes. They are important in the regulation of insulin secretion, the
control of vascular and smooth muscle tone, and the response of the heart
(and possibly the brain) to ischaemia.
 
Channels formed by the association of Kir6.1 (also known as u-KATP-1) with
the SUR2B receptor are sensitive to K+ channel-opening drugs such as
diazoxide and pinacidil, closely resembling the properties of native
channels found in vascular tissues [4].
 
KIR61CHANNEL is a 9-element fingerprint that provides a signature for the
Kir6.1 inward rectifier K+ channel. The fingerprint was derived from an
initial alignment of 3 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise Kir6.1 but distinguish it from other subtypes -
motif 1 resides within the putative cytoplasmic N-terminus; motif 2 lies
between the first TM domain and the pore-forming (H5) domain; and motifs
4-9 encode portions of the cytoplasmic C-terminus. A single iteration on
SPTR37_10f was required to reach convergence, no further sequences being
identified beyond the starting set.
Summary Information
3 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9333333333
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
IRK8_HUMAN    IRK8_MOUSE    IRK8_RAT      
Sequence Titles
IRK8_HUMAN  ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 8 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 8) (UKATP-1) (ATP-SENSITIVE INWARDLY RECTIFYING K+ CHANNEL KIR6.1) - HOMO SAPIENS (HUMAN). 
IRK8_MOUSE ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 8 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 8) (UKATP-1) (ATP-SENSITIVE INWARDLY RECTIFYING K+ CHANNEL KIR6.1) - MUS MUSCULUS (MOUSE).
IRK8_RAT ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 8 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 8) (UKATP-1) (ATP-SENSITIVE INWARDLY RECTIFYING K+ CHANNEL KIR6.1) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
AENLRKPRIRD IRK8_MOUSE 19 19 -
AENLRKPRIRD IRK8_RAT 19 19 -
AENLRKPRIRD IRK8_HUMAN 19 19 -

Motif 2 width=14
Element Seqn Id St Int Rpt
IYAYMEKGTMEKSG IRK8_MOUSE 101 71 -
IYAYMEKGITEKSG IRK8_RAT 101 71 -
IYAYMEKSGMEKSG IRK8_HUMAN 101 71 -

Motif 3 width=12
Element Seqn Id St Int Rpt
LESAVCVTNVRS IRK8_MOUSE 115 0 -
LESAVCVTNVRS IRK8_RAT 115 0 -
LESTVCVTNVRS IRK8_HUMAN 115 0 -

Motif 4 width=9
Element Seqn Id St Int Rpt
ISATDLANQ IRK8_MOUSE 280 153 -
ISATDLVNQ IRK8_RAT 280 153 -
ISATDLANQ IRK8_HUMAN 280 153 -

Motif 5 width=11
Element Seqn Id St Int Rpt
RVAAPRCSARE IRK8_MOUSE 347 58 -
RVAAPRCSARE IRK8_RAT 347 58 -
KVAAPRCSARE IRK8_HUMAN 347 58 -

Motif 6 width=12
Element Seqn Id St Int Rpt
KPSILIQTLQKS IRK8_MOUSE 361 3 -
KPSILIQTLQKS IRK8_RAT 361 3 -
KPSILIQTLQKS IRK8_HUMAN 361 3 -

Motif 7 width=9
Element Seqn Id St Int Rpt
ELSHQNSLR IRK8_MOUSE 373 0 -
ELSHQNSLR IRK8_RAT 373 0 -
ELSHQNSLR IRK8_HUMAN 373 0 -

Motif 8 width=10
Element Seqn Id St Int Rpt
SSLMVPKVQF IRK8_MOUSE 403 21 -
SSLMVPKVQF IRK8_RAT 403 21 -
SSLMVPKVQF IRK8_HUMAN 403 21 -

Motif 9 width=12
Element Seqn Id St Int Rpt
MTPEGNQCPSES IRK8_MOUSE 413 0 -
MTPEGNQCPSES IRK8_RAT 413 0 -
MTPEGNQNTSES IRK8_HUMAN 413 0 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
AENLRKPRIRD IRK8_MOUSE 19 19 -
AENLRKPRIRD IRK8_RAT 19 19 -
AENLRKPRIRD IRK8_HUMAN 19 19 -

Motif 2 width=14
Element Seqn Id St Int Rpt
IYAYMEKGTMEKSG IRK8_MOUSE 101 71 -
IYAYMEKGITEKSG IRK8_RAT 101 71 -
IYAYMEKSGMEKSG IRK8_HUMAN 101 71 -

Motif 3 width=12
Element Seqn Id St Int Rpt
LESAVCVTNVRS IRK8_MOUSE 115 0 -
LESAVCVTNVRS IRK8_RAT 115 0 -
LESTVCVTNVRS IRK8_HUMAN 115 0 -

Motif 4 width=9
Element Seqn Id St Int Rpt
ISATDLANQ IRK8_MOUSE 280 153 -
ISATDLVNQ IRK8_RAT 280 153 -
ISATDLANQ IRK8_HUMAN 280 153 -

Motif 5 width=11
Element Seqn Id St Int Rpt
RVAAPRCSARE IRK8_MOUSE 347 58 -
RVAAPRCSARE IRK8_RAT 347 58 -
KVAAPRCSARE IRK8_HUMAN 347 58 -

Motif 6 width=12
Element Seqn Id St Int Rpt
KPSILIQTLQKS IRK8_MOUSE 361 3 -
KPSILIQTLQKS IRK8_RAT 361 3 -
KPSILIQTLQKS IRK8_HUMAN 361 3 -

Motif 7 width=9
Element Seqn Id St Int Rpt
ELSHQNSLR IRK8_MOUSE 373 0 -
ELSHQNSLR IRK8_RAT 373 0 -
ELSHQNSLR IRK8_HUMAN 373 0 -

Motif 8 width=10
Element Seqn Id St Int Rpt
SSLMVPKVQF IRK8_MOUSE 403 21 -
SSLMVPKVQF IRK8_RAT 403 21 -
SSLMVPKVQF IRK8_HUMAN 403 21 -

Motif 9 width=12
Element Seqn Id St Int Rpt
MTPEGNQCPSES IRK8_MOUSE 413 0 -
MTPEGNQCPSES IRK8_RAT 413 0 -
MTPEGNQNTSES IRK8_HUMAN 413 0 -