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PR01323

Identifier
KIR13CHANNEL  [View Relations]  [View Alignment]  
Accession
PR01323
No. of Motifs
8
Creation Date
12-MAY-2000
Title
Kir1.3 inward rectifer K+ channel signature
Database References
PRINTS; PR00169 KCHANNEL; PR01320 KIRCHANNEL
PRODOM; PD063373; PD063374
Literature References
1. MINOR, D.L., JR., MASSELLING, S.J., JAN, Y.N. AND JAN, L.Y.
Transmembrane structure of an inwardly rectifying potassium channel.
CELL 96 879-891 (1999).
 
2. DOUPNIK, C.A., DAVIDSON, N. AND LESTER, H.A.
The inward rectifier potassium channel family.
CURR.OPIN.NEUROBIOL. 5 268-277 (1995).
 
3. REIMANN, F. AND ASHCROFT, F.M.
Inwardly rectifying potassium channels.
CURR.OPIN.CELL BIOL. 11 503-508 (1999).
 
4. SHUCK, M.E., PISER, T.M., BOCK, J.H., SLIGHTOM, J.L., LEE, K.S. AND
BIENKOWSKI, M.J. 
Cloning and characterization of two K+ inward rectifier (Kir) 1.1 potassium
channel homologs from human kidney (Kir1.2 and Kir1.3). 
J.BIOL.CHEM. 272 586-593 (1997).

Documentation
Potassium channels are found in virtually all cell types. Their pore-
forming subunits fall into three structural families, i.e. those possessing
six, four and two transmembrane (TM) domains. The six-TM domain K+ channels
can be further subdivided into six families: the voltage-gated K+ channels
(Kv), the KCNQ channels, the eag-like K+ channels, and three Ca2+-activated
K+ channels. Inwardly-rectifying K+ channels (Kir) are the principal class
of two-TM domain K+ channels, and the recently-discovered two-pore domain
K+ channels, make up a family of four-TM domain K+ channels.
 
Inwardly rectifying potassium channels (Kir) are responsible for regulating
diverse processes including: cellular excitability, vascular tone, heart
rate, renal salt flow, and insulin release [1]. To date, around twenty
members of this superfamily have been cloned, which can be grouped into six
families by sequence similarity, and these are designated Kir1.x-7.x [2,3].
 
Cloned Kir channel cDNAs encode proteins of between ~370-500 residues,
containing two predicted TM domains, with the characteristic K+ channel
pore-forming domain located between them. Both N- and C-termini are thought
to be cytoplasmic, and the N-terminus lacks a signal sequence. It is thought
that four Kir subunits assemble to form a tetrameric channel complex, which
may be hetero- or homomeric [1].
 
Kir1.3 (also known as Kir4.2) was cloned from human kidney, where it appears
to be found most abundantly. At lower levels, it has also been detected in
the pancreas and lung. It is most closely related to Kir1.2, to which it is
62% identical. Unlike the other Kir1.x family members, in the Kir1.3
sequence, the Walker Type A consensus motif (for nucleotide binding) is
not conserved. Heterologous expression of Kir1.3 in Xenopus oocytes did not
lead to detectable channel activity. Indeed, co-expression of Kir1.3 with
either Kir1.1 or Kir1.2 reduced currents resulting from expression of these
inward rectifier subunits alone, consistent with a dominant negative
influence of Kir1.3 on Kir1.1 and Kir1.2 expression [4].
 
KIR13CHANNEL is an 8-element fingerprint that provides a signature for the
Kir1.3 inward rectifer K+ channel. The fingerprint was derived from an
initial alignment of 3 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise Kir1.3 but distinguish if from other subtypes -
motifs 1-3 lie within the putative cytoplasmic N-terminus; and motifs 4-8
encode portions of the cytoplasmic C-terminus. A single iteration on
SPTR37_10f was required to reach convergence, no further sequences being
identified beyond the starting set.
Summary Information
3 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
833333333
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
IRKF_HUMAN    IRKF_MOUSE    O70339        
Sequence Titles
IRKF_HUMAN  ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 15 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 15) (INWARD RECTIFIER POTASSIUM CHANNEL KIR 4.2) (KIR1.3) - HOMO SAPIENS (HUMAN). 
IRKF_MOUSE ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 15 (POTASSIUM CHANNEL, INWARDLY RECTIFYING, SUBFAMILY J, MEMBER 15) (INWARD RECTIFIER POTASSIUM CHANNEL KIR 4.2) - MUS MUSCULUS (MOUSE).
O70339 INWARDLY RECTIFYING POTASSIUM CHANNEL KIR1.3 - CAVIA PORCELLUS (GUINEA PIG).
Scan History
SPTR37_10f 1  50   NSINGLE    
Initial Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
MDAIHIGMSS IRKF_HUMAN 1 1 -
MDAIHLGMSS IRKF_MOUSE 1 1 -
MDTIHMSVTR O70339 1 1 -

Motif 2 width=10
Element Seqn Id St Int Rpt
KHTAGAGLKA IRKF_HUMAN 15 4 -
KHTNGVGLKA IRKF_MOUSE 15 4 -
KHMAGPGLKT O70339 15 4 -

Motif 3 width=13
Element Seqn Id St Int Rpt
IDKVDGIYLLYLQ IRKF_HUMAN 40 15 -
IDKVDGIYLLYLQ IRKF_MOUSE 40 15 -
IDKVDGIYLLYLQ O70339 40 15 -

Motif 4 width=11
Element Seqn Id St Int Rpt
SHCAVITKQNG IRKF_HUMAN 181 128 -
SHCAVISKQNG IRKF_MOUSE 181 128 -
SHCAVITKQNG O70339 181 128 -

Motif 5 width=11
Element Seqn Id St Int Rpt
ILLNQATVKFH IRKF_HUMAN 228 36 -
ILLNQATVKFH IRKF_MOUSE 228 36 -
ILLNQATVKFH O70339 228 36 -

Motif 6 width=10
Element Seqn Id St Int Rpt
LTPQNLKEKE IRKF_HUMAN 267 28 -
LTPQNLKEKE IRKF_MOUSE 267 28 -
LTPQNLKEKE O70339 267 28 -

Motif 7 width=12
Element Seqn Id St Int Rpt
IRKSPDCTFYCA IRKF_HUMAN 333 56 -
IRKSPDCTFYCA IRKF_MOUSE 333 56 -
IRKSSDCTFYCA O70339 333 56 -

Motif 8 width=20
Element Seqn Id St Int Rpt
RQEDQRERELRTLLLQQSNV IRKF_HUMAN 356 11 -
RQEDQRERELRSLLLQQSNV IRKF_MOUSE 356 11 -
RQEDQRERELRTLLLHQSNV O70339 356 11 -
Final Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
MDAIHIGMSS IRKF_HUMAN 1 1 -
MDAIHLGMSS IRKF_MOUSE 1 1 -
MDTIHMSVTR O70339 1 1 -

Motif 2 width=10
Element Seqn Id St Int Rpt
KHTAGAGLKA IRKF_HUMAN 15 4 -
KHTNGVGLKA IRKF_MOUSE 15 4 -
KHMAGPGLKT O70339 15 4 -

Motif 3 width=13
Element Seqn Id St Int Rpt
IDKVDGIYLLYLQ IRKF_HUMAN 40 15 -
IDKVDGIYLLYLQ IRKF_MOUSE 40 15 -
IDKVDGIYLLYLQ O70339 40 15 -

Motif 4 width=11
Element Seqn Id St Int Rpt
SHCAVITKQNG IRKF_HUMAN 181 128 -
SHCAVISKQNG IRKF_MOUSE 181 128 -
SHCAVITKQNG O70339 181 128 -

Motif 5 width=11
Element Seqn Id St Int Rpt
ILLNQATVKFH IRKF_HUMAN 228 36 -
ILLNQATVKFH IRKF_MOUSE 228 36 -
ILLNQATVKFH O70339 228 36 -

Motif 6 width=10
Element Seqn Id St Int Rpt
LTPQNLKEKE IRKF_HUMAN 267 28 -
LTPQNLKEKE IRKF_MOUSE 267 28 -
LTPQNLKEKE O70339 267 28 -

Motif 7 width=12
Element Seqn Id St Int Rpt
IRKSPDCTFYCA IRKF_HUMAN 333 56 -
IRKSPDCTFYCA IRKF_MOUSE 333 56 -
IRKSSDCTFYCA O70339 333 56 -

Motif 8 width=20
Element Seqn Id St Int Rpt
RQEDQRERELRTLLLQQSNV IRKF_HUMAN 356 11 -
RQEDQRERELRSLLLQQSNV IRKF_MOUSE 356 11 -
RQEDQRERELRTLLLHQSNV O70339 356 11 -