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PR01313

Identifier
P2X6RECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01313
No. of Motifs
9
Creation Date
23-MAR-2000
Title
P2X6 purinoceptor signature
Database References
PRINTS; PR00252 NRIONCHANNEL; PR01307 P2XRECEPTOR
PRODOM; PD188453
Literature References
1. NORTH, R.A.
Families of ion channels with two hydrophobic segments.
CURR.OPIN.CELL BIOL. 8 474-483 (1996).
 
2. MACKENZIE, A.B., SURPRENANT, A. AND NORTH, R.A.
Functional and molecular diversity of purinergic ion channel receptors.
ANN.N.Y.ACAD.SCI. 868 716-729 (1999).

Documentation
P2X purinoceptors are cell membrane ion channels, gated by adenosine
5'-triphosphate (ATP) and other nucleotides. They have been found to be
widely expressed on mammalian cells, and, by means of their functional 
properties, can be differentiated into three sub-groups. The first group is
almost equally well activated by ATP and its analogue alphabetamethyleneATP,
whereas, the second group is not activated by the latter compound. A third
type of receptor (also called P2Z) is distinguished by the fact that
repeated or prolonged agonist application leads to the opening of much
larger pores, allowing large molecules to traverse the cell membrane. This
increased permeability rapidly leads to cell death, and lysis.
 
Molecular cloning studies have identified seven P2X receptor subtypes,
designated P2X1-P2X7. These receptors are proteins that share 35-48% amino
acid identity, and possess two putative transmembrane (TM) domains,
separated by a long (~270 residues) intervening sequence, which is
thought to form an extracellular loop. Around 1/4 of the residues within
the loop are invariant between the cloned subtypes, including 10
characteristic cysteines.
 
Studies of the functional properties of heterologously expressed P2X
receptors, together with the examination of their distribution in native
tissues, suggests they likely occur as both homo- and heteromultimers
in vivo [1,2].
 
The P2X6 receptor (along with P2X2, P2X4 and P2X5) falls into a group of
receptors that are sensitive to ATP, but not alphabetamethyleneATP. There
is some evidence that P2X6 may heteropolymerise with P2X4, since they are
often found together in native tissues, and can be co-immunoprecipitated. 
 
P2X6RECEPTOR is a 9-element fingerprint that provides a signature for the
P2X purinoceptor isoform 6. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections
that characterise the P2X purinoceptor isoform 6 but distinguish it from
others - motifs 1-2 reside within the putative cytoplasmic N-terminus;
motifs 3-6 lie within the exoplasmic loop connecting the two putative TM
domains; motif 7 lies in the centre of the second TM domain; and motifs
9-10 encode portions of the cytoplasmic C-terminus. A single iteration on
SPTR37_10f was required to reach convergence, no further sequences being
identified beyond the starting set.
Summary Information
3 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9333333333
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
P2X6_HUMAN    P2X6_MOUSE    P2X6_RAT      
Sequence Titles
P2X6_HUMAN  P2X PURINOCEPTOR 6 (ATP RECEPTOR) (P2X6) (PURINERGIC RECEPTOR) (P2XM) - HOMO SAPIENS (HUMAN). 
P2X6_MOUSE P2X PURINOCEPTOR 6 (ATP RECEPTOR) (P2X6) (PURINERGIC RECEPTOR) (P2XM) - MUS MUSCULUS (MOUSE).
P2X6_RAT P2X PURINOCEPTOR 6 (ATP RECEPTOR) (P2X6) (PURINERGIC RECEPTOR) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 1  75   NSINGLE    
Initial Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
SWGFLDYKT P2X6_MOUSE 11 11 -
SWGFLDYKT P2X6_RAT 11 11 -
GWGLLDYKT P2X6_HUMAN 9 9 -

Motif 2 width=8
Element Seqn Id St Int Rpt
YVLTRNCR P2X6_MOUSE 22 2 -
YVMTRNCW P2X6_RAT 22 2 -
YVMTRNWR P2X6_HUMAN 20 2 -

Motif 3 width=12
Element Seqn Id St Int Rpt
EHPSVPLANCWA P2X6_MOUSE 121 91 -
EHPSVPLANCWA P2X6_RAT 121 91 -
EHPSVPLANCWV P2X6_HUMAN 119 91 -

Motif 4 width=8
Element Seqn Id St Int Rpt
TVTFSKFN P2X6_MOUSE 195 62 -
TVTFNKFN P2X6_RAT 195 62 -
TVTFSKFN P2X6_HUMAN 193 62 -

Motif 5 width=12
Element Seqn Id St Int Rpt
SNALLTWDNTYF P2X6_MOUSE 206 3 -
TNALDTWDNTYF P2X6_RAT 206 3 -
SNALETWDPTYF P2X6_HUMAN 204 3 -

Motif 6 width=10
Element Seqn Id St Int Rpt
GGDFEDLALL P2X6_MOUSE 243 25 -
GGDFEDLALL P2X6_RAT 243 25 -
GGTFEDLALL P2X6_HUMAN 241 25 -

Motif 7 width=11
Element Seqn Id St Int Rpt
TGAAWLGMVTF P2X6_MOUSE 335 82 -
TGAAWLGMVTF P2X6_RAT 335 82 -
TGAAWLGVVTF P2X6_HUMAN 333 82 -

Motif 8 width=11
Element Seqn Id St Int Rpt
VDREAGFYWRT P2X6_MOUSE 354 8 -
VDREAGFYWRT P2X6_RAT 354 8 -
VDREAHFYWRT P2X6_HUMAN 352 8 -

Motif 9 width=13
Element Seqn Id St Int Rpt
YEEARAPKTTTNS P2X6_MOUSE 366 1 -
YEEARAPKATTNS P2X6_RAT 366 1 -
YEEAKAPKATANS P2X6_HUMAN 364 1 -
Final Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
SWGFLDYKT P2X6_MOUSE 11 11 -
SWGFLDYKT P2X6_RAT 11 11 -
GWGLLDYKT P2X6_HUMAN 9 9 -

Motif 2 width=8
Element Seqn Id St Int Rpt
YVLTRNCR P2X6_MOUSE 22 2 -
YVMTRNCW P2X6_RAT 22 2 -
YVMTRNWR P2X6_HUMAN 20 2 -

Motif 3 width=12
Element Seqn Id St Int Rpt
EHPSVPLANCWA P2X6_MOUSE 121 91 -
EHPSVPLANCWA P2X6_RAT 121 91 -
EHPSVPLANCWV P2X6_HUMAN 119 91 -

Motif 4 width=8
Element Seqn Id St Int Rpt
TVTFSKFN P2X6_MOUSE 195 62 -
TVTFNKFN P2X6_RAT 195 62 -
TVTFSKFN P2X6_HUMAN 193 62 -

Motif 5 width=12
Element Seqn Id St Int Rpt
SNALLTWDNTYF P2X6_MOUSE 206 3 -
TNALDTWDNTYF P2X6_RAT 206 3 -
SNALETWDPTYF P2X6_HUMAN 204 3 -

Motif 6 width=10
Element Seqn Id St Int Rpt
GGDFEDLALL P2X6_MOUSE 243 25 -
GGDFEDLALL P2X6_RAT 243 25 -
GGTFEDLALL P2X6_HUMAN 241 25 -

Motif 7 width=11
Element Seqn Id St Int Rpt
TGAAWLGMVTF P2X6_MOUSE 335 82 -
TGAAWLGMVTF P2X6_RAT 335 82 -
TGAAWLGVVTF P2X6_HUMAN 333 82 -

Motif 8 width=11
Element Seqn Id St Int Rpt
VDREAGFYWRT P2X6_MOUSE 354 8 -
VDREAGFYWRT P2X6_RAT 354 8 -
VDREAHFYWRT P2X6_HUMAN 352 8 -

Motif 9 width=13
Element Seqn Id St Int Rpt
YEEARAPKTTTNS P2X6_MOUSE 366 1 -
YEEARAPKATTNS P2X6_RAT 366 1 -
YEEAKAPKATANS P2X6_HUMAN 364 1 -