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PR01308

Identifier
P2X1RECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01308
No. of Motifs
10
Creation Date
16-MAR-2000
Title
P2X1 purinoceptor signature
Database References
PRINTS; PR00252 NRIONCHANNEL; PR01307 P2XRECEPTOR
PRODOM; PD153085
Literature References
1. NORTH, R.A.
Families of ion channels with two hydrophobic segments.
CURR.OPIN.CELL BIOL. 8 474-483 (1996).
 
2. MACKENZIE, A.B., SURPRENANT, A. AND NORTH, R.A.
Functional and molecular diversity of purinergic ion channel receptors.
ANN.N.Y.ACAD.SCI. 868 716-729 (1999).

Documentation
P2X purinoceptors are cell membrane ion channels, gated by adenosine
5'-triphosphate (ATP) and other nucleotides. They have been found to be
widely expressed on mammalian cells, and, by means of their functional 
properties, can be differentiated into three sub-groups. The first group is
almost equally well activated by ATP and its analogue alphabetamethyleneATP,
whereas, the second group is not activated by the latter compound. A third
type of receptor (also called P2Z) is distinguished by the fact that
repeated or prolonged agonist application leads to the opening of much
larger pores, allowing large molecules to traverse the cell membrane. This
increased permeability rapidly leads to cell death, and lysis.
 
Molecular cloning studies have identified seven P2X receptor subtypes,
designated P2X1-P2X7. These receptors are proteins that share 35-48% amino
acid identity, and possess two putative transmembrane (TM) domains,
separated by a long (~270 residues) intervening sequence, which is
thought to form an extracellular loop. Around 1/4 of the residues within
the loop are invariant between the cloned subtypes, including 10
characteristic cysteines.
 
Studies of the functional properties of heterologously expressed P2X
receptors, together with the examination of their distribution in native
tissues, suggests they likely occur as both homo- and heteromultimers
in vivo [1,2].
 
Expression of P2X1 receptors produces receptors that are equally well
activated by alphabetamethyleneATP and ATP. The properties of the currents
so generated are quite comparable to those determined for native P2X
receptors, such as those found to be present in smooth muscle [2].
 
P2X1RECEPTOR is a 10-element fingerprint that provides a signature for the
P2X purinoceptor isoform 1. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections
that characterise the P2X purinoceptor isoform 1 but distinguish it from
others - motif 1 resides within the putative cytoplasmic N-terminus; motifs
2-7 lie within the exoplasmic loop connecting the two putative TM domains; 
motif 8 spans the end of the exoplasmic loop, and the start of the
cytoplasmic C-terminus; and motifs 9-10 encode portions of the cytoplasmic
C-terminus. A single iteration on SPTR37_10f was required to reach
convergence, no further sequences being identified beyond the starting set.
Summary Information
3 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
103333333333
90000000000
80000000000
70000000000
60000000000
50000000000
40000000000
30000000000
20000000000
12345678910
True Positives
P2X1_HUMAN    P2X1_MOUSE    P2X1_RAT      
Sequence Titles
P2X1_HUMAN  P2X PURINOCEPTOR 1 (ATP RECEPTOR) (P2X1) (PURINERGIC RECEPTOR) - HOMO SAPIENS (HUMAN). 
P2X1_MOUSE P2X PURINOCEPTOR 1 (ATP RECEPTOR) (P2X1) (PURINERGIC RECEPTOR) - MUS MUSCULUS (MOUSE).
P2X1_RAT P2X PURINOCEPTOR 1 (ATP RECEPTOR) (P2X1) (PURINERGIC RECEPTOR) (RP-2 PROTEIN) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 1  50   NSINGLE    
Initial Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
QDELSAFFF P2X1_MOUSE 6 6 -
QDELSAFFF P2X1_RAT 6 6 -
QEELAAFLF P2X1_HUMAN 6 6 -

Motif 2 width=10
Element Seqn Id St Int Rpt
SVKLKGLAVT P2X1_MOUSE 66 51 -
SVKLKGLAVT P2X1_RAT 66 51 -
SVKLKGLAVT P2X1_HUMAN 66 51 -

Motif 3 width=12
Element Seqn Id St Int Rpt
QGHCAENPEGGI P2X1_MOUSE 114 38 -
QGHCAENPEGGI P2X1_RAT 114 38 -
QGYCAEHPEGGI P2X1_HUMAN 114 38 -

Motif 4 width=10
Element Seqn Id St Int Rpt
GKAERKAQGI P2X1_MOUSE 135 9 -
GKAERKAQGI P2X1_RAT 135 9 -
GKAKRKAQGI P2X1_HUMAN 135 9 -

Motif 5 width=11
Element Seqn Id St Int Rpt
KVNRRNLVEEV P2X1_MOUSE 199 54 -
KVNRRNLVEEV P2X1_RAT 199 54 -
KVNRRNLVEEV P2X1_HUMAN 199 54 -

Motif 6 width=11
Element Seqn Id St Int Rpt
SLGYVVRESGQ P2X1_MOUSE 231 21 -
NLGYVVRESGQ P2X1_RAT 231 21 -
QLGYVVQESGQ P2X1_HUMAN 231 21 -

Motif 7 width=9
Element Seqn Id St Int Rpt
TNRRHLFKV P2X1_MOUSE 302 60 -
TNRRHLFKV P2X1_RAT 302 60 -
TNYRHLFKV P2X1_HUMAN 302 60 -

Motif 8 width=12
Element Seqn Id St Int Rpt
LHILPKRHYYKQ P2X1_MOUSE 354 43 -
LHILPKRHYYKQ P2X1_RAT 354 43 -
LHILPKRHYYKQ P2X1_HUMAN 354 43 -

Motif 9 width=15
Element Seqn Id St Int Rpt
FKYAEDMGPGEGERD P2X1_MOUSE 368 2 -
FKYAEDMGPGEGEHD P2X1_RAT 368 2 -
FKYAEDMGPGAAERD P2X1_HUMAN 368 2 -

Motif 10 width=16
Element Seqn Id St Int Rpt
AATSSTLGLQENMRTS P2X1_MOUSE 384 1 -
VATSSTLGLQENMRTS P2X1_RAT 384 1 -
AATSSTLGLQENMRTS P2X1_HUMAN 384 1 -
Final Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
QDELSAFFF P2X1_MOUSE 6 6 -
QDELSAFFF P2X1_RAT 6 6 -
QEELAAFLF P2X1_HUMAN 6 6 -

Motif 2 width=10
Element Seqn Id St Int Rpt
SVKLKGLAVT P2X1_MOUSE 66 51 -
SVKLKGLAVT P2X1_RAT 66 51 -
SVKLKGLAVT P2X1_HUMAN 66 51 -

Motif 3 width=12
Element Seqn Id St Int Rpt
QGHCAENPEGGI P2X1_MOUSE 114 38 -
QGHCAENPEGGI P2X1_RAT 114 38 -
QGYCAEHPEGGI P2X1_HUMAN 114 38 -

Motif 4 width=10
Element Seqn Id St Int Rpt
GKAERKAQGI P2X1_MOUSE 135 9 -
GKAERKAQGI P2X1_RAT 135 9 -
GKAKRKAQGI P2X1_HUMAN 135 9 -

Motif 5 width=11
Element Seqn Id St Int Rpt
KVNRRNLVEEV P2X1_MOUSE 199 54 -
KVNRRNLVEEV P2X1_RAT 199 54 -
KVNRRNLVEEV P2X1_HUMAN 199 54 -

Motif 6 width=11
Element Seqn Id St Int Rpt
SLGYVVRESGQ P2X1_MOUSE 231 21 -
NLGYVVRESGQ P2X1_RAT 231 21 -
QLGYVVQESGQ P2X1_HUMAN 231 21 -

Motif 7 width=9
Element Seqn Id St Int Rpt
TNRRHLFKV P2X1_MOUSE 302 60 -
TNRRHLFKV P2X1_RAT 302 60 -
TNYRHLFKV P2X1_HUMAN 302 60 -

Motif 8 width=12
Element Seqn Id St Int Rpt
LHILPKRHYYKQ P2X1_MOUSE 354 43 -
LHILPKRHYYKQ P2X1_RAT 354 43 -
LHILPKRHYYKQ P2X1_HUMAN 354 43 -

Motif 9 width=15
Element Seqn Id St Int Rpt
FKYAEDMGPGEGERD P2X1_MOUSE 368 2 -
FKYAEDMGPGEGEHD P2X1_RAT 368 2 -
FKYAEDMGPGAAERD P2X1_HUMAN 368 2 -

Motif 10 width=16
Element Seqn Id St Int Rpt
AATSSTLGLQENMRTS P2X1_MOUSE 384 1 -
VATSSTLGLQENMRTS P2X1_RAT 384 1 -
AATSSTLGLQENMRTS P2X1_HUMAN 384 1 -