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PR01259

Identifier
NACAEXCHNGR  [View Relations]  [View Alignment]  
Accession
PR01259
No. of Motifs
4
Creation Date
06-DEC-1999
Title
Na+/Ca2+ exchanger signature
Database References

PFAM; PF01699 Na_Ca_Ex
PRODOM; PD004396; PD003955; PD004181; PD149743
INTERPRO; IPR002613
Literature References
1. NICOLL, D.A., LONGONI, S. AND PHILIPSON, K.D.
Molecular cloning and functional expression of the cardiac sarcolemmal
Na+-Ca2+ exchanger.
SCIENCE 250 562-565 (1990).
 
2. LI, Z., MATSUOKA, S., HRYSHKO, L.V., NICOLL, D.A., BERSOHN, M.M.,
BURKE, E.P., LIFTON, R.P. AND PHILIPSON, K.D.
Cloning of the NCX2 isoform of the plasma membrane Na+/Ca2+ exchanger.
J.BIOL.CHEM. 269 17434-17439 (1994).
 
3. NICOLL, D.A., QUEDNAU, B.D., QUI, Z., XIA, Y-R., LUSIS, A.J. AND
PHILIPSON, K.D.
Cloning of a third mammalian Na+-Ca2+ exchanger, NCX3.
J.BIOL.CHEM. 271 24914-24921 (1996).

Documentation
Na+/Ca2+ exchange proteins are involved in maintaining Ca2+ homeostasis in
a wide variety of cell types. They are found in both the plasma membrane
and intracellular organellar membranes, where they exchange Na+ for Ca2+ in
an electrogenic manner. When located in the plasma membrane, they generally
utilise the transmembrane (TM) Na+ concentration gradient in order to
extrude Ca2+ from cells. Three mammalian isoforms have been cloned to date
(NCX1-3), which consist of 920-970 amino acid residues that are predicted
to possess 11 or 12 TM domains. Interestingly, they possess a short motif 
(~30 residues) that is similar to the Na+/K+-ATPase, although its function
is unknown [1-3].
 
NCX1 has been found to be predominantly expressed in the heart, where it
plays an important role in excitation-contraction coupling, but it is also
abundant in a variety of other tissues [1]. NCX2 and NCX3 transcripts have
been detected in the brain and skeletal muscle [2,3]. Homologous Na+/Ca2+
exchange proteins have also been found in C.elegans, D.melanogaster and
squid.
 
NACAEXCHNGR is a 4-element fingerprint that provides a signature for
Na+/Ca2+ exchangers. The fingerprint was derived from an initial alignment
of 9 sequences: the motifs were drawn from the C-terminal hydrophobic
region of the alignment - motif 1 encodes most of the seventh putative
TM domain; motif 2 spans the end of the seventh TM domain, through the 
start of the eighth TM domain; motif 3 lies within the eighth putative TM
region; and motif 4 encodes ~half the extracellular loop between TM domains
8 and 9. Two iterations on SPTR37_10f were required to reach convergence,
at which point a true set comprising 19 sequences was identified.
Summary Information
19 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
419191919
30000
20000
1234
True Positives
NAC1_BOVIN    NAC1_CANFA    NAC1_CAVPO    NAC1_FELCA    
NAC1_HUMAN NAC1_MOUSE NAC1_RAT NAC2_RAT
NAC3_RAT O02196 O18367 O35157
O45630 O97801 Q21609 Q21895
Q24413 Q28662 Q94161
Sequence Titles
NAC1_BOVIN  SODIUM/CALCIUM EXCHANGER 1 PRECURSOR (NA+/CA2+-EXCHANGE PROTEIN 1) - BOS TAURUS  
NAC1_CANFA SODIUM/CALCIUM EXCHANGER 1 PRECURSOR (NA+/CA2+-EXCHANGE PROTEIN 1) - CANIS FAMIL
NAC1_CAVPO SODIUM/CALCIUM EXCHANGER 1 PRECURSOR (NA+/CA2+-EXCHANGE PROTEIN 1) - CAVIA PORCE
NAC1_FELCA SODIUM/CALCIUM EXCHANGER 1 PRECURSOR (NA+/CA2+-EXCHANGE PROTEIN 1) - FELIS SILVE
NAC1_HUMAN SODIUM/CALCIUM EXCHANGER 1 PRECURSOR (NA+/CA2+-EXCHANGE PROTEIN 1) - HOMO SAPIEN
NAC1_MOUSE SODIUM/CALCIUM EXCHANGER 1 PRECURSOR (NA+/CA2+-EXCHANGE PROTEIN 1) - MUS MUSCULU
NAC1_RAT SODIUM/CALCIUM EXCHANGER 1 PRECURSOR (NA+/CA2+-EXCHANGE PROTEIN 1) - RATTUS NORV
NAC2_RAT SODIUM/CALCIUM EXCHANGER 2 PRECURSOR (NA+/CA2+-EXCHANGE PROTEIN 2) - RATTUS NORV
NAC3_RAT SODIUM/CALCIUM EXCHANGER 3 PRECURSOR (NA+/CA2+-EXCHANGE PROTEIN 3) - RATTUS NORV
O02196 NA/CA EXCHANGER - LOLIGO OPALESCENS (CALIFORNIA MARKET SQUID).
O18367 CALX PRECURSOR - DROSOPHILA MELANOGASTER (FRUIT FLY).
O35157 SOLUTE CARRIER FAMILY 8 (SODIUM/CALCIUM EXCHANGER), MEMBER 1 - MUS MUSCULUS (MOU
O45630 SODIUM-CALCIUM EXCHANGER - CAENORHABDITIS ELEGANS.
O97801 SODIUM-CALCIUM EXCHANGER ISOFORM NCX1.3 - MACACA MULATTA (RHESUS MACAQUE).
Q21609 SODIUM-CALCIUM EXCHANGER - CAENORHABDITIS ELEGANS.
Q21895 ZC168.1 PROTEIN - CAENORHABDITIS ELEGANS.
Q24413 NA/CA EXCHANGE PROTEIN - DROSOPHILA MELANOGASTER (FRUIT FLY).
Q28662 RENAL NA/CA EXCHANGER NACA-2 - ORYCTOLAGUS CUNICULUS (RABBIT).
Q94161 SIMILAR TO SODIUM/CALCIUM EXCHANGER PROTEIN 1 (SODIUM-CALCIUM EXCHANGER) - CAENO
Scan History
SPTR37_10f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
IVSILMIGLLTAF NAC1_CAVPO 802 802 -
IVSILMIGLLTAF Q28662 773 773 -
IVSILMIGLLTAF O35157 802 802 -
IVSILMIGLLTAF NAC1_RAT 803 803 -
IVSILMIGLLTAF NAC1_MOUSE 802 802 -
IVSILMIGLLTAF NAC1_HUMAN 802 802 -
IVSILMIGLLTAF NAC1_BOVIN 802 802 -
VVSILIIGMLTAI NAC3_RAT 759 759 -
GVCILVIGLLTAL NAC2_RAT 753 753 -

Motif 2 width=12
Element Seqn Id St Int Rpt
IGDLASHFGCTI NAC1_CAVPO 815 0 -
IGDLASHFGCTI Q28662 786 0 -
IGDLASHFGCTI O35157 815 0 -
IGDLASHFGCTI NAC1_RAT 816 0 -
IGDLASHFGCTI NAC1_MOUSE 815 0 -
IGDLASHFGCTI NAC1_HUMAN 815 0 -
IGDLASHFACTI NAC1_BOVIN 815 0 -
IGDLASHFGCTI NAC3_RAT 772 0 -
IGDLASHFGCTV NAC2_RAT 766 0 -

Motif 3 width=13
Element Seqn Id St Int Rpt
LKDSVTAVVFVAL NAC1_CAVPO 828 1 -
LKDSVTAVVFVAL Q28662 799 1 -
LKDSVTAVVFVAL O35157 828 1 -
LKDSVTAVVFVAL NAC1_RAT 829 1 -
LKDSVTAVVFVAL NAC1_MOUSE 828 1 -
LKDSVTAVVFVAL NAC1_HUMAN 828 1 -
LKDSVTAVVFVAL NAC1_BOVIN 828 1 -
LKDSVTAVVFVAF NAC3_RAT 785 1 -
LKDSVNAVVFVAL NAC2_RAT 779 1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
GTSVPDTFASKVAA NAC1_CAVPO 841 0 -
GTSVPDTFASKVAA Q28662 812 0 -
GTSVPDTFASKVAA O35157 841 0 -
GTSVPDTFASKVAA NAC1_RAT 842 0 -
GTSVPDTFASKVAA NAC1_MOUSE 841 0 -
GTSVPDTFASKVAA NAC1_HUMAN 841 0 -
GTSVPDTFASKVAA NAC1_BOVIN 841 0 -
GTSVPDTFASKAAA NAC3_RAT 798 0 -
GTSIPDTFASKVAA NAC2_RAT 792 0 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
IVSILMIGLLTAF NAC1_CAVPO 802 802 -
IVSILMIGLLTAF Q28662 773 773 -
IVSILMIGLLTAF O97801 766 766 -
IVSILMIGLLTAF O35157 802 802 -
IVSILMIGLLTAF NAC1_RAT 803 803 -
IVSILMIGLLTAF NAC1_MOUSE 802 802 -
IVSILMIGLLTAF NAC1_HUMAN 802 802 -
IVSILMIGILTAF NAC1_CANFA 802 802 -
IVSILMIGILTAF NAC1_FELCA 802 802 -
IVSILMIGLLTAF NAC1_BOVIN 802 802 -
VVSILIIGMLTAI NAC3_RAT 759 759 -
GVCILVIGLLTAL NAC2_RAT 753 753 -
TVSIILIGVLTAF O02196 724 724 -
VVAIFMIGVLTAV O45630 757 757 -
VVAIFMIGVLTAV Q94161 742 742 -
VVAIAMIGLLTAF Q21609 711 711 -
VVSIFVIGVITAI O18367 782 782 -
VVSIFVIGVITAI Q24413 782 782 -
VVALIGIGLVTAV Q21895 638 638 -

Motif 2 width=12
Element Seqn Id St Int Rpt
IGDLASHFGCTI NAC1_CAVPO 815 0 -
IGDLASHFGCTI Q28662 786 0 -
IGDLASHFGCTI O97801 779 0 -
IGDLASHFGCTI O35157 815 0 -
IGDLASHFGCTI NAC1_RAT 816 0 -
IGDLASHFGCTI NAC1_MOUSE 815 0 -
IGDLASHFGCTI NAC1_HUMAN 815 0 -
IGDLASHFGCTI NAC1_CANFA 815 0 -
IGDLASHFGCTI NAC1_FELCA 815 0 -
IGDLASHFACTI NAC1_BOVIN 815 0 -
IGDLASHFGCTI NAC3_RAT 772 0 -
IGDLASHFGCTV NAC2_RAT 766 0 -
IGDLATYFGCTI O02196 737 0 -
VGDLASQFGCWV O45630 770 0 -
VGDLASQFGCWV Q94161 755 0 -
IGDIAAAFGCTV Q21609 724 0 -
IGDAASYFGCAL O18367 795 0 -
IGDAASYFGCAL Q24413 795 0 -
VGDVASIFGCMV Q21895 651 0 -

Motif 3 width=13
Element Seqn Id St Int Rpt
LKDSVTAVVFVAL NAC1_CAVPO 828 1 -
LKDSVTAVVFVAL Q28662 799 1 -
LKDSVTAVVFVAL O97801 792 1 -
LKDSVTAVVFVAL O35157 828 1 -
LKDSVTAVVFVAL NAC1_RAT 829 1 -
LKDSVTAVVFVAL NAC1_MOUSE 828 1 -
LKDSVTAVVFVAL NAC1_HUMAN 828 1 -
LKDSVTAVVFVAL NAC1_CANFA 828 1 -
LKDSVTAVVFVAL NAC1_FELCA 828 1 -
LKDSVTAVVFVAL NAC1_BOVIN 828 1 -
LKDSVTAVVFVAF NAC3_RAT 785 1 -
LKDSVNAVVFVAL NAC2_RAT 779 1 -
LKDAVTAVSFVAL O02196 750 1 -
LKDAVTAISFVAL O45630 783 1 -
LKDAVTAISFVAL Q94161 768 1 -
LKDSVTALTLVAM Q21609 737 1 -
IKDSVTAILFVAL O18367 808 1 -
IKDSVTAILFVAL Q24413 808 1 -
LKDAVTAITLVAL Q21895 664 1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
GTSVPDTFASKVAA NAC1_CAVPO 841 0 -
GTSVPDTFASKVAA Q28662 812 0 -
GTSVPDTFASKVAA O97801 805 0 -
GTSVPDTFASKVAA O35157 841 0 -
GTSVPDTFASKVAA NAC1_RAT 842 0 -
GTSVPDTFASKVAA NAC1_MOUSE 841 0 -
GTSVPDTFASKVAA NAC1_HUMAN 841 0 -
GTSVPDTFASKVAA NAC1_CANFA 841 0 -
GTSVPDTFASKVAA NAC1_FELCA 841 0 -
GTSVPDTFASKVAA NAC1_BOVIN 841 0 -
GTSVPDTFASKAAA NAC3_RAT 798 0 -
GTSIPDTFASKVAA NAC2_RAT 792 0 -
GTSVPDTFASKVAA O02196 763 0 -
GTSVPDTFASKVSA O45630 796 0 -
GTSVPDTFASKVSA Q94161 781 0 -
GTSLPDTFASRTAA Q21609 750 0 -
GTSIPDTFASMIAA O18367 821 0 -
GTSIPDTFASMIAA Q24413 821 0 -
GTSLPDTFASKIAA Q21895 677 0 -