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PR01232

Identifier
NAHCO3TRSPRT  [View Relations]  [View Alignment]  
Accession
PR01232
No. of Motifs
7
Creation Date
06-DEC-1999
Title
Na+/HCO3- co-transporter signature
Database References

PRODOM; PD018437; PD018439
INTERPRO; IPR003024
Literature References
1. BORON, W.F., FONG, P., HEDIGER, M.A., BOULPAEP, E.L. AND ROMERO, M.F.
The electrogenic Na/HCO3 cotransporter.
WIEN.KLIN.WOCHENSCHR. 109 445-456 (1997).
 
2. ROMERO, M.F., HEDIGER, M.A., BOULPAEP, E.L. AND BORON W.F.
Expression cloning and characterization of a renal eletrogenic Na+/HCO3-
cotransporter.
NATURE 387 409-413 (1997).
 
3. BURNHAM, C.E., HASSANE, A., WANG, Z., SHULL, G.E. AND SOLEIMANI, M.
Cloning and functional expression of a human kidney Na+:HCO3-
cotransporter.
J.BIOL.CHEM. 272 19111-19114 (1997).
 
4. ABULADZE, N., LEE, I., NEWMAN, D., HWANG, J., BOORER, K., PUSHKIN, A.
AND KURTZ, I.
Molecular cloning, chromosomal localization, tissue distribution, and
functional expression of the human pancreatic sodium bicarbonate
cotransporter.
J.BIOL.CHEM. 273 17689-17695 (1998).

Documentation
Na+/HCO3- co-transport proteins are involved in cellular HCO3- absorption
and secretion, and also with intracellular pH regulation. They mediate the
coupled movement of Na+ and HCO3- across plasma membranes in most of the
cell types so far investigated. A single HCO3- is transported together with
one to three Na+; this transport mode is therefore often electrogenic. In
the kidney, an electrogenic Na+/HCO3- co-transporter is the principal HCO3-
transporter of the renal proximal tubule, and is responsible for
reabsorption of more than 85% of the filtered load of HCO3- [1]. Until
recently, the molecular nature of these Na+/HCO3- co-transporters had
remained undiscovered, as initial attempts to clone them based on presumed
homology to Cl-/HCO3- (anion) exchangers had proved unsuccessful. Instead,
an expression cloning strategy was successfully utilised to identify the
Na+/HCO3- co-transporter from salamander kidney, an organ previously found
to possess electrogenic Na+/HCO3- co-transport activity [2]. The cloned co-
transporter consists of 1035 amino acids, and shows ~25-30% sequence
identity to anion exchanger proteins, which is distributed along the entire
sequence length. They also share very similar hydropathy plots, suggesting
they have ~10 transmembrane (TM) domains.
 
At least 3 mammalian Na+/HCO3- co-transporters have since been cloned, with
similar primary sequence lengths and putative membrance topologies. One of
these has been found to be a kidney-specific isoform [3], which is near-
identical (except for a varying N-terminal region) to a more widely-
distributed co-transporter cloned from pancreatic tissue [4].
 
NAHCO3TRSPRT is a 7-element fingerprint that provides a signature for
Na+/HCO3- co-transporters. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved regions 
spanning the N-terminal half of the alignment; motifs 1-5 lie in the
putative cytoplasmic N-terminus; motif 6 spans the end of the second
putative TM domain, through the start of the third TM domain; and motif
7 lies at the end of the fourth TM domain. Two iterations on SPTR37_10f
were required to reach convergence, at which point a true set comprising
10 sequences was identified.
Summary Information
10 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
710101010101010
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
O13134        O15153        O35422        O54815        
O60350 O61304 O88343 O95233
O97915 Q20642
Sequence Titles
O13134      ELECTROGENIC NA+ BICARBONATE COTRANSPORTER - AMBYSTOMA TIGRINUM (TIGER SALAMANDE 
O15153 SODIUM BICARBONATE COTRANSPORTER - HOMO SAPIENS (HUMAN).
O35422 SODIUM BICARBONATE COTRANSPORTER - RATTUS NORVEGICUS (RAT).
O54815 ELECTROGENIC NA+ BICARBONATE COTRANSPORTER - RATTUS NORVEGICUS (RAT).
O60350 SODIUM BICARBONATE COTRANSPORTER2 - HOMO SAPIENS (HUMAN).
O61304 HCO3 TRANSPORTER - CAENORHABDITIS ELEGANS.
O88343 PANCREAS SODIUM BICARBONATE COTRANSPORTER - MUS MUSCULUS (MOUSE).
O95233 SODIUM BICARBONATE COTRANSPORTER - HOMO SAPIENS (HUMAN).
O97915 SODIUM BICARBONATE COTRANSPORTER - ORYCTOLAGUS CUNICULUS (RABBIT).
Q20642 F52B5.1 PROTEIN - CAENORHABDITIS ELEGANS.
Scan History
SPTR37_10f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=7
Element Seqn Id St Int Rpt
VEQGGER O15153 94 94 -
VEQGGER O88343 138 138 -
VEQGGER O54815 94 94 -
VEQGGER O35422 94 94 -
VEQGGER O13134 95 95 -
VEDGGDR O60350 52 52 -
VEQGGNR O61304 68 68 -

Motif 2 width=9
Element Seqn Id St Int Rpt
LGPKGKAKS O15153 284 183 -
LGPKGKAKS O88343 328 183 -
LGPKGKAKS O54815 284 183 -
LGPKGKAKS O35422 284 183 -
LGPKGKAKS O13134 285 183 -
LGPAGKAPQ O60350 258 199 -
LGPTGHGQQ O61304 285 210 -

Motif 3 width=9
Element Seqn Id St Int Rpt
GIDEFLDEV O15153 325 32 -
GIDEFLDEV O88343 369 32 -
GIDEFLDEV O54815 325 32 -
GIDEFLDEV O35422 325 32 -
GIDEFLDEV O13134 326 32 -
GIDEFLDQV O60350 299 32 -
GIDEFLDQV O61304 326 32 -

Motif 4 width=9
Element Seqn Id St Int Rpt
VLPPGEWDP O15153 335 1 -
VLPPGEWDP O88343 379 1 -
VLPPGEWDP O54815 335 1 -
VLPPGEWDP O35422 335 1 -
VLPLGEWDP O13134 336 1 -
VLPPGEWDP O60350 309 1 -
VLPPGEWDP O61304 336 1 -

Motif 5 width=13
Element Seqn Id St Int Rpt
IRIEPPKSLPSSD O15153 345 1 -
IRIEPPKSLPSSD O88343 389 1 -
IRIEPPKSLPSSD O54815 345 1 -
IRIEPPKSLPSSD O35422 345 1 -
IRIEPPKSLPSSD O13134 346 1 -
IRIEPPKSVPSQE O60350 319 1 -
IRIEPPSKLPSQE O61304 346 1 -

Motif 6 width=8
Element Seqn Id St Int Rpt
LTILSSTG O15153 479 121 -
LTILSSTG O88343 523 121 -
LTILSSTG O54815 479 121 -
LTILSSTG O35422 479 121 -
LTILSSTG O13134 478 119 -
LTILGSTG O60350 448 116 -
LTIVGSTG O61304 482 123 -

Motif 7 width=8
Element Seqn Id St Int Rpt
ATDASFLV O15153 526 39 -
ATDASFLV O88343 570 39 -
ATDASFLV O54815 526 39 -
ATDASFLV O35422 526 39 -
ATDASFLV O13134 525 39 -
ATDASSLV O60350 495 39 -
VTDASALV O61304 529 39 -
Final Motifs
Motif 1  width=7
Element Seqn Id St Int Rpt
VEQGGER O15153 94 94 -
VEQGGER O97915 94 94 -
VEQGGER O88343 138 138 -
VEQGGER O54815 94 94 -
VEQGGER O35422 94 94 -
VEQGGER O13134 95 95 -
VEDGGER O95233 146 146 -
VEDGGDR O60350 52 52 -
VEQGGNR O61304 68 68 -
VEQGGNR Q20642 110 110 -

Motif 2 width=9
Element Seqn Id St Int Rpt
LGPKGKAKS O15153 284 183 -
LGPKGKAKS O97915 284 183 -
LGPKGKAKS O88343 328 183 -
LGPKGKAKS O54815 284 183 -
LGPKGKAKS O35422 284 183 -
LGPKGKAKS O13134 285 183 -
LGPVGKGQQ O95233 344 191 -
LGPAGKAPQ O60350 258 199 -
LGPTGHGQQ O61304 285 210 -
LGPTGHGQQ Q20642 327 210 -

Motif 3 width=9
Element Seqn Id St Int Rpt
GIDEFLDEV O15153 325 32 -
GIDEFLDEV O97915 325 32 -
GIDEFLDEV O88343 369 32 -
GIDEFLDEV O54815 325 32 -
GIDEFLDEV O35422 325 32 -
GIDEFLDEV O13134 326 32 -
GIDEFLDQV O95233 385 32 -
GIDEFLDQV O60350 299 32 -
GIDEFLDQV O61304 326 32 -
GIDEFLDQV Q20642 368 32 -

Motif 4 width=9
Element Seqn Id St Int Rpt
VLPPGEWDP O15153 335 1 -
VLPPGEWDP O97915 335 1 -
VLPPGEWDP O88343 379 1 -
VLPPGEWDP O54815 335 1 -
VLPPGEWDP O35422 335 1 -
VLPLGEWDP O13134 336 1 -
VLPPGEWDP O95233 395 1 -
VLPPGEWDP O60350 309 1 -
VLPPGEWDP O61304 336 1 -
VLPPGEWDP Q20642 378 1 -

Motif 5 width=13
Element Seqn Id St Int Rpt
IRIEPPKSLPSSD O15153 345 1 -
IRIEPPKSLPSSD O97915 345 1 -
IRIEPPKSLPSSD O88343 389 1 -
IRIEPPKSLPSSD O54815 345 1 -
IRIEPPKSLPSSD O35422 345 1 -
IRIEPPKSLPSSD O13134 346 1 -
IRIEPPKNVPSQE O95233 405 1 -
IRIEPPKSVPSQE O60350 319 1 -
IRIEPPSKLPSQE O61304 346 1 -
IRIEPPSKLPSQE Q20642 388 1 -

Motif 6 width=8
Element Seqn Id St Int Rpt
LTILSSTG O15153 479 121 -
LTILSSTG O97915 479 121 -
LTILSSTG O88343 523 121 -
LTILSSTG O54815 479 121 -
LTILSSTG O35422 479 121 -
LTILSSTG O13134 478 119 -
LTILGSTG O95233 532 114 -
LTILGSTG O60350 448 116 -
LTIVGSTG O61304 482 123 -
LTIVGSTG Q20642 524 123 -

Motif 7 width=8
Element Seqn Id St Int Rpt
ATDASFLV O15153 526 39 -
ATDASFLV O97915 526 39 -
ATDASFLV O88343 570 39 -
ATDASFLV O54815 526 39 -
ATDASFLV O35422 526 39 -
ATDASFLV O13134 525 39 -
ATDASSLV O95233 579 39 -
ATDASSLV O60350 495 39 -
VTDASALV O61304 529 39 -
VTDASALV Q20642 571 39 -