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PR01213

Identifier
BSYNUCLEIN  [View Relations]  [View Alignment]  
Accession
PR01213
No. of Motifs
4
Creation Date
02-OCT-1999
Title
Beta-synuclein signature
Database References
PRINTS; PR01211 SYNUCLEIN
INTERPRO; IPR002461
Literature References
1. LAVEDAN, C.
The synuclein family.
GENOME RES. 8 871-880 (1998).
 
2. TOBE, T., NAKAJO, S., TANAKA, A., MITOYA, A., OMATA, K., NAKAYA, K.,
TOMITA, M. AND NAKAMURA, Y.
Cloning and characterization of the cDNA encoding a novel brain-specific
14kDa protein.
J.NEUROCHEM. 59 1624-1629 (1992).
 
3. GEORGE, J.M., JIN, H., WOODS, W.S. AND CLAYTON, D.F.
Characterization of a novel protein regulated during the critical period
for song learning in the zebra finch.
NEURON 15 361-372 (1995).
 
4. NAKAJO, S., TSUKADA, K., OMATA, K., NAKAMURA, Y. AND NAKAYA, K.
A new brain-specific 14kDa protein is a phosphoprotein. Its complete amino 
acid sequence and evidence for phosphorylation.
EUR.J.BIOCHEMISTRY 217 1057-1063 (1993). 

Documentation
Human synuclein has been shown to belong to a gene family [1]. To date, 
three different proteins, termed alpha, beta and gamma, have been 
characterised. The amino acid sequences of synucleins share a similar
overall structure: hydropathy analysis reveals a central hydrophobic domain
of ~30 amino acids, flanked by highly hydrophilic stretches; the N-terminal
flanking region contains four repeating motifs (EKTKEGV); and the C-terminal
domain is rich in glutamic acid and proline [2]
 
Synelfin, a homologue of synuclein, is a soluble presynaptic protein that 
is predicted to form an amphipathic alpha-helical structure typical of the
lipid-binding domain in apolipoproteins [3]. This protein is believed to
serve a novel function critical to the regulation of vertebrate neural
plasticity [3]. 
 
Beta-synuclein is expressed in the brain, specifically in synapses around
neurons, but not in glial cells [4]. The protein, which has been designated
a phosphoneuroprotein, has been found to be phosphorylated in vitro and in
vivo [4]. It is believed that the physiological functions of beta-synuclein
may be controlled by the phosphorylation reaction. 
 
BSYNUCLEIN is a 4-element fingerprint that provides a signature for
beta-synucleins. The fingerprint was derived from an initial alignment of
3 sequences: the motifs were drawn from short conserved regions spanning
virtually the full alignment length, focusing on those sections that
characterise the beta-synucleins but distinguish them from the rest of the
synuclein family - motif 1 lies in the N-terminal region of the hydrophobic
domain; and motifs 2-4 span the Glu-Pro-rich C-terminal domain. A single
iteration on SPTR37_10f was required to reach convergence, no further
sequences being identified beyond the starting set.
Summary Information
3 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
43333
30000
20000
1234
True Positives
SYUB_BOVIN    SYUB_HUMAN    SYUB_RAT      
Sequence Titles
SYUB_BOVIN  BETA-SYNUCLEIN (PHOSPHONEUROPROTEIN 14) (PNP 14) (14 KD BRAIN- SPECIFIC PROTEIN) 
SYUB_HUMAN BETA-SYNUCLEIN - HOMO SAPIENS (HUMAN).
SYUB_RAT BETA-SYNUCLEIN (PHOSPHONEUROPROTEIN 14) (PNP 14) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_10f 1  30   NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
ASHLGGAVFSGAG SYUB_RAT 63 63 -
ASHLGGAVFSGAG SYUB_HUMAN 63 63 -
ASHLGGAVFSGAG SYUB_BOVIN 63 63 -

Motif 2 width=16
Element Seqn Id St Int Rpt
KEEFPTDLKPEEVAQE SYUB_RAT 86 10 -
REEFPTDLKPEEVAQE SYUB_HUMAN 86 10 -
KEEFPTDLKPEEVAQE SYUB_BOVIN 86 10 -

Motif 3 width=14
Element Seqn Id St Int Rpt
EAAEEPLIEPLMEP SYUB_RAT 101 -1 -
EAAEEPLIEPLMEP SYUB_HUMAN 101 -1 -
EAAEEPLIEPLMEP SYUB_BOVIN 101 -1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
PEGESYEDSPQEEY SYUB_RAT 114 -1 -
PEGESYEDPPQEEY SYUB_HUMAN 114 -1 -
PEGESYEEQPQEEY SYUB_BOVIN 114 -1 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
ASHLGGAVFSGAG SYUB_RAT 63 63 -
ASHLGGAVFSGAG SYUB_HUMAN 63 63 -
ASHLGGAVFSGAG SYUB_BOVIN 63 63 -

Motif 2 width=16
Element Seqn Id St Int Rpt
KEEFPTDLKPEEVAQE SYUB_RAT 86 10 -
REEFPTDLKPEEVAQE SYUB_HUMAN 86 10 -
KEEFPTDLKPEEVAQE SYUB_BOVIN 86 10 -

Motif 3 width=14
Element Seqn Id St Int Rpt
EAAEEPLIEPLMEP SYUB_RAT 101 -1 -
EAAEEPLIEPLMEP SYUB_HUMAN 101 -1 -
EAAEEPLIEPLMEP SYUB_BOVIN 101 -1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
PEGESYEDSPQEEY SYUB_RAT 114 -1 -
PEGESYEDPPQEEY SYUB_HUMAN 114 -1 -
PEGESYEEQPQEEY SYUB_BOVIN 114 -1 -