SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01156

Identifier
PACAPRECEPTR  [View Relations]  [View Alignment]  
Accession
PR01156
No. of Motifs
10
Creation Date
23-MAY-1999
Title
Pituitary adenylate cyclase activating polypeptide type 1 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00249 GPCRSECRETIN
PRINTS; PR00491 VASOACTVEIPR
INTERPRO; IPR002285
GCRDB; GCR_0397; GCR_0652; GCR_0774; GCR_0904; GCR_1888; GCR_1565

GCRDB; GCR_0369
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.   
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.  
Molecular cloning and expression of a cDNA encoding the secretin receptor.
EMBO J. 10 1635-1641 (1991).
 
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H.,
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor.
SCIENCE 254 1022-1024 (1991). 
 
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F.,
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
 
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
 
6. WATSON, S. AND ARKINSTALL, S.
Vasoactive intestinal polypeptide family.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.278-283.
 
7. WEI, Y. AND MOJSOV, S.
Tissue specific expression of different human receptor types for pituitary
adenylate cyclase activating polypeptide and vasoactive intestinal 
polypeptide: implications for their role in human physiology.
J.NEUROENDOCRINOL. 8 811-817 (1996). 
 
8. PISEGNA, J.R. AND WANK, S.A.
Molecular cloning and functional expression of the pituitary adenylate
cyclase-activating polypeptide type I receptor.
PROC.NATL.ACAD.SCI.U.S.A. 90 6345-6349 (1993). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that   
encompasses a wide range of functions (including various autocrine, para-   
crine and endocrine processes). They show considerable diversity at the   
sequence level, on the basis of which they can be separated into distinct   
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,   
but between which there is no statistically significant similarity in   
sequence [1]. The currently known clan members include the rhodopsin-like   
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating   
pheromone receptors, and the metabotropic glutamate receptor family.       
 
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid   
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-   
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a manner
reminiscent of the rhodopsins and other receptors believed to interact with
G proteins. However, while a similar 3D framework has been proposed to
account for this, there is no significant sequence identity between these
families: the secretin-like receptors thus bear their own unique `7TM'
signature.      
 
Vasoactive intestinal polypeptide (VIP) has a wide physiological profile.
In the periphery, it induces relaxation in smooth muscle; inhibits
secretion in certain tissues, but stimulates secretion in others; and
modulates activity of cells in the immune system [6]. In the CNS, it has a
range of both excitatory and inhibitory actions. VIP receptors are
distributed widely in the periphery, and occur throughout the gastro-
intestinal tract and genitourinary system, other smooth muscles and
secretory glands. In the CNS, they are found abundantly in, e.g. the cortex,
hippocampus and thalamus [6]. All VIP receptors activate adenylyl cyclase.
 
There are two structurally distinct receptors that recognise pituitary
adenylate cyclase activating polypeptide (PACAP) and VIP peptides with
similar affinities (PACAP/VIPR-1, PACAP/VIPR-2), as well as a specific
receptor for the PACAP peptide (PACAP-1) [7]. PACAP type I receptors are
present in the hypothalamus and anterior pituitary, where they regulate the 
release of adrenocorticotropin, luteinising hormone, growth hormone and
prolactin, and in the adrenal medulla, where they regulate the release of
epinephrine [8]. The receptors are also found in high concentrations in 
testicular germ cells, where they may regulate spermatogenesis, and in some
transformed cell lines, such as the rat pancreatic acinar carcinoma cell
AR4-2J [8].
 
PACAPRECEPTR is a 10-element fingerprint that provides a signature for 
pituitary adenylate cyclase activating polypeptide receptors. The 
fingerprint was derived from an initial alignment of 4 sequences: the motifs
were drawn from conserved regions spanning the full alignment length,
focusing on those areas that characterise the type 1 PACAP receptors but
distinguish them from the rest of the VIP receptor family - motifs 1-5 lie
in the N-terminal region immediately preceding TM domain 1; motif 6 lies in
the loop between TM domains 2 and 3; motif 7 spans the loop between TM
domains 6 and 7; and motifs 8-10 reside at the C-terminus. A single
iteration on SPTR37_9f was required to reach convergence, no further 
sequences being identified beyond the starting set. Two partial matches
were found: O73769 is a goldfish PACAP type 1 receptor that matches motifs
6-10; and VIPR_CARAU is a goldfish type 1 VIP receptor that matches motifs
7 and 9.
Summary Information
   4 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
104444444444
90000000000
80000000000
70000000000
60000000000
50000011111
40000000000
30000000000
20000001010
12345678910
True Positives
PACR_BOVIN    PACR_HUMAN    PACR_MOUSE    PACR_RAT      
True Positive Partials
Codes involving 5 elements
O73769
Codes involving 2 elements
VIPR_CARAU
Sequence Titles
PACR_BOVIN  PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE I RECEPTOR PRECURSOR (PACAP TYPE I RECEPTOR) (PACAP-R-1) - BOS TAURUS (BOVINE). 
PACR_HUMAN PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE I RECEPTOR PRECURSOR (PACAP TYPE I RECEPTOR) (PACAP-R-1) - HOMO SAPIENS (HUMAN).
PACR_MOUSE PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE I RECEPTOR PRECURSOR (PACAP TYPE I RECEPTOR) (PACAP-R-1) - MUS MUSCULUS (MOUSE).
PACR_RAT PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE IA RECEPTOR PRECURSOR (PACAP TYPE IA RECEPTOR) (PACAP-R-1) - RATTUS NORVEGICUS (RAT).

O73769 PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE 1 RECEPTOR PRECURSOR - CARASSIUS AURATUS (GOLDFISH).

VIPR_CARAU VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR (VIP-R) (VIP RECEPTOR) - CARASSIUS AURATUS (GOLDFISH).
Scan History
SPTR37_9f  1  50   NSINGLE    
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
PMAIAMHSDCIFKKEQAMCLER PACR_MOUSE 16 16 -
PVAIAMHSDCIFKKEQAMCLER PACR_RAT 15 15 -
PMATAMHSDCIFKKEQAMCLEK PACR_BOVIN 33 33 -
PMAPAMHSDCIFKKEQAMCLEK PACR_HUMAN 16 16 -

Motif 2 width=18
Element Seqn Id St Int Rpt
QRANDLMGLNESSPGCPG PACR_MOUSE 39 1 -
QRANDLMGLNESSPGCPG PACR_RAT 38 1 -
QRVNDLMGLNDSSPGCPG PACR_BOVIN 56 1 -
QRANELMGFNDSSPGCPG PACR_HUMAN 39 1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
EVFRIFNPDQVWMTETIGD PACR_MOUSE 79 22 -
EVFRIFNPDQVWMTETIGD PACR_RAT 78 22 -
ELFRIFNPDQVWETETIGE PACR_BOVIN 96 22 -
ELFRIFNPDQVWETETIGE PACR_HUMAN 79 22 -

Motif 4 width=18
Element Seqn Id St Int Rpt
FADSNSLEITDMGVVGRN PACR_MOUSE 100 2 -
FADSNSLEITDMGVVGRN PACR_RAT 99 2 -
FADSKSLDLSDMRVVSRN PACR_BOVIN 117 2 -
FGDSNSLDLSDMGVVSRN PACR_HUMAN 100 2 -

Motif 5 width=20
Element Seqn Id St Int Rpt
GFDDYEPESGDQDYYYLSVK PACR_MOUSE 135 17 -
GFDDYEPESGDQDYYYLSVK PACR_RAT 134 17 -
GFEEYESETGDQDYYYLSVK PACR_BOVIN 152 17 -
GFDEYESETGDQDYYYLSVK PACR_HUMAN 135 17 -

Motif 6 width=17
Element Seqn Id St Int Rpt
YAEQDSSHCFVSTVECK PACR_MOUSE 211 56 -
YAEQDSSHCFVSTVECK PACR_RAT 210 56 -
YAEQDSNHCFVSTVECK PACR_BOVIN 228 56 -
YAEQDSNHCFISTVECK PACR_HUMAN 211 56 -

Motif 7 width=15
Element Seqn Id St Int Rpt
FSPENVSKRERLVFE PACR_MOUSE 399 171 -
FSPENVSKRERLVFE PACR_RAT 426 199 -
FSPENVSKRERLVFE PACR_BOVIN 416 171 -
FSPENVSKRERLVFE PACR_HUMAN 371 143 -

Motif 8 width=13
Element Seqn Id St Int Rpt
WRSWKVNRYFTMD PACR_MOUSE 443 29 -
WRSWKVNRYFTMD PACR_RAT 470 29 -
WRSWKVNRYFTMD PACR_BOVIN 460 29 -
WRSWKVNRYFAVD PACR_HUMAN 415 29 -

Motif 9 width=17
Element Seqn Id St Int Rpt
HRHPSLASSGVNGGTQL PACR_MOUSE 458 2 -
HRHPSLASSGVNGGTQL PACR_RAT 485 2 -
HRHPSLASSGVNGGTQL PACR_BOVIN 475 2 -
HRHPSLASSGVNGGTQL PACR_HUMAN 430 2 -

Motif 10 width=22
Element Seqn Id St Int Rpt
LSILSKSSSQLRMSSLPADNLA PACR_MOUSE 474 -1 -
LSILSKSSSQLRMSSLPADNLA PACR_RAT 501 -1 -
LSILSKSSSQIRMSGLPADNLA PACR_BOVIN 491 -1 -
LSILSKSSSQIRMSGLPADNLA PACR_HUMAN 446 -1 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
PMAIAMHSDCIFKKEQAMCLER PACR_MOUSE 16 16 -
PVAIAMHSDCIFKKEQAMCLER PACR_RAT 15 15 -
PMATAMHSDCIFKKEQAMCLEK PACR_BOVIN 33 33 -
PMAPAMHSDCIFKKEQAMCLEK PACR_HUMAN 16 16 -

Motif 2 width=18
Element Seqn Id St Int Rpt
QRANDLMGLNESSPGCPG PACR_MOUSE 39 1 -
QRANDLMGLNESSPGCPG PACR_RAT 38 1 -
QRVNDLMGLNDSSPGCPG PACR_BOVIN 56 1 -
QRANELMGFNDSSPGCPG PACR_HUMAN 39 1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
EVFRIFNPDQVWMTETIGD PACR_MOUSE 79 22 -
EVFRIFNPDQVWMTETIGD PACR_RAT 78 22 -
ELFRIFNPDQVWETETIGE PACR_BOVIN 96 22 -
ELFRIFNPDQVWETETIGE PACR_HUMAN 79 22 -

Motif 4 width=18
Element Seqn Id St Int Rpt
FADSNSLEITDMGVVGRN PACR_MOUSE 100 2 -
FADSNSLEITDMGVVGRN PACR_RAT 99 2 -
FADSKSLDLSDMRVVSRN PACR_BOVIN 117 2 -
FGDSNSLDLSDMGVVSRN PACR_HUMAN 100 2 -

Motif 5 width=20
Element Seqn Id St Int Rpt
GFDDYEPESGDQDYYYLSVK PACR_MOUSE 135 17 -
GFDDYEPESGDQDYYYLSVK PACR_RAT 134 17 -
GFEEYESETGDQDYYYLSVK PACR_BOVIN 152 17 -
GFDEYESETGDQDYYYLSVK PACR_HUMAN 135 17 -

Motif 6 width=17
Element Seqn Id St Int Rpt
YAEQDSSHCFVSTVECK PACR_MOUSE 211 56 -
YAEQDSSHCFVSTVECK PACR_RAT 210 56 -
YAEQDSNHCFVSTVECK PACR_BOVIN 228 56 -
YAEQDSNHCFISTVECK PACR_HUMAN 211 56 -

Motif 7 width=15
Element Seqn Id St Int Rpt
FSPENVSKRERLVFE PACR_MOUSE 399 171 -
FSPENVSKRERLVFE PACR_RAT 426 199 -
FSPENVSKRERLVFE PACR_BOVIN 416 171 -
FSPENVSKRERLVFE PACR_HUMAN 371 143 -

Motif 8 width=13
Element Seqn Id St Int Rpt
WRSWKVNRYFTMD PACR_MOUSE 443 29 -
WRSWKVNRYFTMD PACR_RAT 470 29 -
WRSWKVNRYFTMD PACR_BOVIN 460 29 -
WRSWKVNRYFAVD PACR_HUMAN 415 29 -

Motif 9 width=17
Element Seqn Id St Int Rpt
HRHPSLASSGVNGGTQL PACR_MOUSE 458 2 -
HRHPSLASSGVNGGTQL PACR_RAT 485 2 -
HRHPSLASSGVNGGTQL PACR_BOVIN 475 2 -
HRHPSLASSGVNGGTQL PACR_HUMAN 430 2 -

Motif 10 width=22
Element Seqn Id St Int Rpt
LSILSKSSSQLRMSSLPADNLA PACR_MOUSE 474 -1 -
LSILSKSSSQLRMSSLPADNLA PACR_RAT 501 -1 -
LSILSKSSSQIRMSGLPADNLA PACR_BOVIN 491 -1 -
LSILSKSSSQIRMSGLPADNLA PACR_HUMAN 446 -1 -